Objective To investigate the changes of myocardial combination enzyme in the early stages of gunshot wound of dogs in seawater for exploring early treatment protocol. Methods Fourteen conventional Beagles models undergoing gunshot wound in seawater were used along with another 3 dogs receiving the wound on land to serve as control. After the dogs were rescued from the seawater, the origin of blood coming from central venous pressure tube was measured (33.75?25.29) min before and after injured for indexes such as alkaline aminotransferase (ALT) , aspartate aminotransferase (AST) , creatine kinase (CK) , creatine kinase isoenzymes - MB (CK -MB) , lactate dehydrogenase (LDH) and adenosine monophosphate (AMY) in the early stages of wound, followed by statistical analysis of the results. Results There was an increase in blood CK (P0.05). As compared with the control, the experimental group in seawater was observed to have significant difference in blood AMY (P 0.05). But it was noted that CK, CK - MB , LDH , AST and ALT contents tended to increase. Conclusion The functions of respiratory, heart, liver and kidney were injured and restrained in Beagle dogs undergoing the firearms wound in natural seawater. The immediate diagnosis and treatment of the organism as well as the out - of - wound - tract injury shoud do while the firearms primal wound tract were treated in the early stages of gunshot wound.

To investigate the efficacy of the formula composed of the herbal drugs to soothe the liver, regulate qi, clarify the heart and eliminate vexation plus acupuncture for general anxiety disorder. Methods: Forty cases in the treatment group were treated with acupuncture plus herbal formula and 38 cases in the control group were treated with oral Doxepin. The assessment was conducted by Self-rating Anxiety Scale (SAS) and the Treatment Emergent Symptom Scale (TESS) before, during and after treatment. Results: The total effective rate was respectively 82.50% in the treatment group and 84.21% in the control group, with a significant difference in SAS assessment (P＜0.01) between the two groups before and after the treatment.There were no significant differences in the curative and remarkable effective rate, total effective rate and SAS assessment between the two groups (P＞0.05), but the side effect was higher in the control group than in the treatment group (P＜0.01). Conclusion: Acupuncture plus herbal formula can have a precise effective effect for extensive anxiety neurosis with mild toxic side effects.

Bacteremia ; etiology ; Catheterization ; adverse effects ; Child ; Coagulase ; metabolism ; Cross Infection ; etiology ; Drug Resistance, Bacterial ; drug effects ; Endocarditis, Bacterial ; etiology ; Humans ; Methicillin ; pharmacology ; Quinolones ; pharmacology ; Staphylococcal Infections ; complications ; drug therapy ; microbiology ; Staphylococcus ; classification ; drug effects ; pathogenicity ; Urinary Tract Infections ; etiology ; Vancomycin ; pharmacology

Colitis, Ulcerative ; prevention & control ; therapy ; Humans ; Integrative Medicine ; Remission Induction

According to the requirement of simulated actual combat,based on the traditional contents of sanitary reconnaissance of water source,this paper introduced some practical knowledge and skills in drinking water under emergency,including water source search,utilization,drinking principles and some measures to reduce the loss of body water.These practices enriched and consum- mated the subject of sanitary reconnaissance of water source.

Objective To research on the applicational value of quantitative immunofluorescence assay of high-sensitivity C- reactive protein(HS-CRP) detection in surveillance of ankylosing spondylitis(AS) and Rheumatoid arthritis(RA).Methods Using quantitative immunofluorescence technique to detect the serum HS-CRP among 43 patients with AS and 62 patients with RA and 30 healthy controls.Results Within-run CV% of repeatability were 4.98% and3.68% for lower and higher concentration of HS- CRP,respectively;between-run CV% of the stability were 4.57% and 5.42% for lower and higher concentration of HS-CRP, respectively;There was a good correlation between results of quantitative immunofluorescence assay and BNP special protein analyzer method(r=0.997);Both the level and the positive rate of HS-CRP of patients with AS and patients with RA were higher than those of the controls in the active phase.The level and the positive rate of HS-CRP in patients with AS and patients with RA were obviously higher in the active phase than in the non-active phase(P0.05).Conclusion There were significant correlations between HS-CRP and the activity of AS and RA,So HS-CRP was useful tools for monitoring the changes of patient's condition.It doesn't have much significance in differential diagnosis of AS and RA.

Occupational Medicine ; education

OBJECTIVEThe clinical effect of the Shi's cervical reduction technique for cervical spondylosis and related disorders has confirmed, however, there were few studies on the body motion during manipulation in vivo study. This study is to summary the law of motion and the motion characteristics of the right operation shoulder, elbow, knee and ankle joints by data acquisition and analysis with the 3D motion capture system.

METHODSThe markers were pasted on the head, trunk, left and right acromion, elbow joint, wrist joint inner side and the outer side of the inner and the outer side and the lateral upper arm, forearm lateral, anterior superior iliac spine, posterior superior iliac spine, trochanter, femoral and tibial tubercle, inner and outer side of knee, ankle, fibular head, medial and lateral in first, 2,5 metatarsal head, heel and dual lateral thigh the calf, lateral tibia of one manipulation practioner, and the subject accepted a complete cycle of cervical "Jin Chu Cao and Gu Cuo Feng" manipulation which was repeated five times. The movement trajectory of the practioner's four markers of operation joints were captured, recorded, calculated and analyzed.

RESULTSThe movement trajectories of four joints were consistent, while the elbow joint had the biggest discrete degree. The 3D activities of the shoulder and elbow were more obvious than other two joints, but the degree of flexion and extension in the knee was significantly greater than the rotation and lateral bending.

CONCLUSIONThe flexibility of upper limb joint and stability of lower limb joint are the important guarantees for the Shi's cervical reduction technique, and the right knee facilitated the exerting force of upper limb by the flexion and extension activities. The 3D model built by the motion capture system would provide a new idea for manipulation teaching and further basic biomechanical research.

Objective To compare stability between the traditional endoscopic technique and thetibial inlay in treatment of posterior cruciate ligament (PCL) reconstruction.Methods Between April 2005 and December 2009,135 patients underwent surgery for PCL in multiple-ligament injuries,and only 88 (65.2％) patients were followed up longer than 2 years.The follow-up time ranged 24 to 77 months (mean,45.9 months).Fifty-seven cases (64.8％) were treated with endoscopic transtibial PCL reconstructions,and 31(35.2％) received tibial inlay.Each patient was evaluated using the Tegner,Lysholm,and American Academy of Orthopaedic Surgeons (AAOS) knee-rating scales,KT-1000 arthrometry and Telos.Results The preoperative KT-1000 measurement was (13.5±4.8) mm (transtibial) and (13.7±5.2) mm (inlay).The postoperative KT-1000 measurement was (2.4±3.4) mm (transtibial) and (2.2±3.6) mm (inlay).The preoperative Telos measurement was (14.9±7.1) mm (transtibial) and (14.9±5.9) mm (inlay).The postoperative Telos measurement was (4.6±4.0) mm (transtibial) and (4.3±3.9) mm (inlay).There were significant differences (P＜0.01) in KT-1000 and Telos between pre-and post-operative measurement,while it does not reach statistical significance (P=0.880,0.956,0.744,0.647) in KT-1000 and Telos when comparing transtibial and inlay technique.There were no significant differences in sex,age,time from injury to surgery,combined injuries or Lysholm,Tegner,AAOS knee score between two groups.Conclusion There was no important advantage of one technique over the other.Transtibial or inlay technique for PCL reconstruction can restore anteroposterior knee stability.

Objective To investigate the clinical features and molecular characteristics of bacteremia caused by K. pneumoniae and study on the differences between classical and hypervirulent K. pneumonia( hvKP) . Methods The clinical features of 70 cases of K. pneumoniae bacteremia collected from Beijing Chaoyang Hospital from 2008 to 2012 were retrospectively analyzed. The patients were identified according to the records from the Clinical Microbiology Department. Patients′ data were obtained from medical records. The molecular characteristics of the K. pneumoniae strains were also studied using PCR, multilocus sequence typing and pulsed field gel electrophoresis( PFGE) methods. Data were analyzed using the statistical package SPSS for windows version 17. 0. For categorical data, different groups were compared using the Chi-square test to analyze the quantitative variables. P≤0. 05 was considered to be statistically significant. All susceptibility data were analyzed using Whonet, version 5. 6. Results The hvKP was identified from 31. 4% of the patients with K. pneumoniae bacteremia, which displayed 4 serotypes ( K1, K2, K20, and K57). About 40. 9% (9/22) hypervirulent strains were isolated from patients without underlying diseases, while 95. 8% (46/48) of the classic K. pneumoniae infections were found in patients with the presence of one or more underlying diseases. More hvKP positive patients (95. 5%, 21/22) had community-acquired infections compared with classical K. pneumonia (cKP) infected patients (35. 4%, 17/48). There was a significant difference between these two groups (χ2 =21. 912,P <0. 001). Two ESBLs-producing hvKP strains were found. The 22 hvKP isolates had 14 different PFGE patterns, among which 6 isolates ( patients with primary liver abscess) shared similar PFGE patterns. Conclusions The
emerging hvKP was prevalent in patients with severe community-acquired infections in healthy individuals. Two ESBLs-producing hvKP strains were identified. Drug resistance had a rising trend, which called for more attention in clinical settings.