The coronary collateral circulation is an alternative source of blood supply to the myocardium jeopardized by failure of the original vessel to provide adequate flow. Angiogenesis growth factors induce angiogenesis to promote collateral circulation, been demonstrated in a number of different animal test and phase Ⅰ clinical experience to induce collateralization in the ischemic area and improve cardiac function. Although there are several unsolved questions, the pharmacologic use of angiogenesis growth factors will represent a viable therapeutic alternative for the patients with ischemic heart disease.

By generalizing the integrity crisis of college students,the article analyzes the causes of this phenomenon,and proposes a series of measures aiming at strengthening integrity education,establishing the favorable environment,completing the mechanism of rewards and punishments and building the credit system,in the hope of securing the deepening and full completion of good-faith education at college,both ideologically and systematically.

OBJECTIVE:To research the correlation between per capita disposable income,investment of pharmaceutical advertisement,people’s healthy level,pharmaceutical price and the demand of drugs in China METHODS:Based on the economical theory,the methods of the econometrics and the Eviews were adopted to establish the model of demand function RESULTS & CONCLUSION:Under the background of the non-full competition,the improvement of people’s living conditions can lead to the demand of drugs The changes of drug price have little influence on the demand of drugs

Objective To investigate the serum levels of anti-BRAF antibody in patients with rheumatoid arthritis (RA) and its clinical significance.Methods Blood samples from 129 patients with RA,71 patients with other rheumatic diseases and 68 healthy controls were measured by enzyme-linked immunosorbent assays (ELISA).The serum levels of anti-BRAF antibody in patients with RA were divided into high,medium and low groups,each group had 43 cases.The clinical symptoms,immunological findings and related imaging data of all cases were recorded.We investigated the relationship between the serum levels of anti-BRAF antibody and the clinical symptoms,immunological findings and related imaging changes of the RA group.Chi-square test,Mann-Whitney U test,Spearman's correlation test were used for statistical analysis.Results ① The serum levels of anti-BRAF antibody were significantly higher in RA group [A:1.093 (0.150-3.210)] than in other rheumatic diseases group [A:0.278 (0.093-0.455)] and healthy controls [A:0.202 (0.121-0.443)] (Z=-11.223,P=0.021 ; Z=-7.839,P=0.008 7).The serum levels of anti-BRAF antibody was not significantly different between patients with other rheumatic diseases group and healthy controls (Z=-0.905,P=0.445).② The difference in positive rate of rheumatoid factor (RF) in different groups of anti-BRAF antibody levels was statistically significant (λx2=10.14,P=0.006),namely the positive rate was significantlyhigher in the low-level group (88％) than in the high-level group (58％) (x2=10.03,P=0.002).Thepositive rate of anti-cyclic citrullinated peptide antibody and anti-keratin antibody of the three groups was not significantly different (x2=0.721,P=0.697; x2=1.735,P=0.188).③ There was significant negative correlation between the levels of anti-BRAF antibody and the course of disease (r=-0.233,P=0.047),RF(r=-2.84,P=0.039),IgM (r=-0.234,P=0.038),and hemoglobin (r=-0.287,P=0.032).There was no significant correlation between the level of anti-BRAF antibody and the joint swelling index (r=-0.133,P=0.230),joint pain index (r=-0.173,P=0.100),other clinical and laboratory parameters.There was significantly negative correlation between the ESR and hemoglobin level.④ There was no significant difference of the level of anti-BRAF antibody between the normal group and patients with bone destruction in the RA group (Z=-0.642,P=0.521).⑤ There was no significant difference in the level of anti-BRAF antibody between the normal group and patients with interstitial pulmonary fibrosis group in the RA group (Z=-0.121,P=0.904).Conclusion The anti-BRAF antibody can be used for the diagnosis of RA as a new autoantibody,and it can also be used in the diagnosis of early or RF-negative RA.There is no correlation between the level of anti-BRAF antibody and bone destruction and interstitial pulmonary fibrosis.

Objective To study on the correlation of hepatic fibrosis with angiotensin Ⅱ (Ang Ⅱ ) in the hepatitis B patients . Methods The electrochemical luminescence and the rate method were adopted to detect hyaluronidase (HA) and Ang Ⅱ levels in 92 cases of acute hepatitis B (acute group) ,108 cases of chronic hepatitis B(chronic group) and 50 healthy people(control group) , and the detected results were performed the correlation analysis .Results Compared with the acute group and the control group ,the HA and Ang Ⅱ levels in the chronic group were increased significantly (P<0 .05) .The HA and Ang Ⅱ levels had statistical differ-ence between the acute group and the control group (P<0 .05) .Further analysis showed that there was a positive correlation be-tween HA and Ang Ⅱ levels(r=0 .629 ,P<0 .05) .Conclusion There the HA level is closely related with the Ang Ⅱ level ,and both of them participate in the process of liver fibrosis ,their levels have the guiding significance in evaluation of severity of liver fi-brosis in the patients with hepatitis B .

Objective To find the inpact(s) of Losartan on myocardial apoptosis in rats with heart failure. Methods Eight-week-old male SD rats were divided into control group (group C) , heart failure (group HF) and treatment group (group LS). Use of doxorubicin injection model, group LS at the same time in a row to the oral losartan, myocardial ultrastructure was examined by TEM. DNA-situ terminal labeling (TUNEL method) was used to detect myocardial apoptosis and myocardial BAX, BCL-2 protein expression was detected by immunohistochemistry. Results Group HF showed significant myocardial cell injury, and apoptotic bodies were found, serum CPK, CK-MB and LDH were increased. In LS group, myocardial apoptosis index was lower (P <0. 01) , myocardial cells BCL-2 expression, BAX expression reduced as compared with those in HF group. Conclusion Losartan can effectively inhibit the process of heart failure occurred in cardiomyocyte apoptosis, reverse myocardial damage and improve the prognosis of heart failure.

Lysyal oxidase (LOX),a cooper dependent monoamine oxidase,can stabilize the extracellular matrix and is closely related to the tumor cell differentiation,vicious transformation and invasiveness.LOX is secreted into extracellular,and it is resolved into a small fragment by sol protease,which is lysyal oxidase propeptide (LOX-PP).According to research,LOX-PP has its own structure and physiological functions,which can inhibit the growth of tumor cells.LOX-PP also plays a significant role in the generation and progression of tumor,which is likely to be a new therapeutic target.

Objective To investigate the characteristics of P3a and P3b which are the subcomponents of P300 event-related potential in patients with vascular cognitive impairment (VCI) ,and to investigate the relations between P3a,P3b and the changes of cognitive function and cerebral blood flow. Methods Thirty patients with VCI and twenty two normal subjects were tested for P3a and P3b cognitive function ischemic focus and cerebral blood flow and then analyzed for correlation. Results (1) The latency of P3a ((446 ± 71) ms, (429 ± 67) ms, (446±61)ms,(428±66)ms)and P3b((496 ±73)ms,(478 ±75)ms,(498 ±77)ms,(483 ±77)ms) was significantly prolonged in patients with VCI compared with that in normal subjects in all recorded regions(P<0.01) . The amplitude of P3a in patients with VCI ((3.3 ±2.6)μV,(3.3 ± 1.8)μV) was significantly lower than that in normal subjects in Fz and Pz (P<0.01) ,and the amplitude of P3b in patients with VCI((4.5 ±2.5)μV,(6.4 ± 3.8)μV,(5.3 ±3.8)μV,(5. 3 ±3.0)μV) was significantly lower than that in normal subjects in all recorded regions (P < 0.01 or P<0.05) . (2) The latency of P3a and P3b in patients with VCI was negatively related to the scores of sub items of attention and MMSE total scores (P < 0.05 or P < 0.01), the amplitude of P3a was positively related to the scores of MMSE total scores(P<0.05) ,the amplitude of P3b was positively related to the scores of attention and MMSE total scores(P<0.05). (3) The latency of P3a in patients with VCI was positively related to frontal lobe(P<0.05) ,and the latency of P3b was positively related to parietal lobe(P<0.05). (4) The latency of P3a in patients with VCI was positively related to RILMCA,RILACA and RIRACA(P<0.05 orP<0.01) ,the latency of P3b was positively related to RILMCA,RIRMCA and RIRACA(P<0.05 orP<0.01) ,and the amplitude of P3a was positively related to RILACA(P<0.01). Conclusion P3a and P3b are good objective indexes for testing the descent of cognitive function in patients with VCI,which can provide reference basis for the clinical diagnosis.

Fetuin A is a kind of glycoprotein that is synthesized and secreted by the liver.It is a natural inhibitor of insulin receptor tyrosine kinase,which is involved in the formation of insulin resistance.It is closely related to the nonalcoholic fatty liver disease.This paper reviews the related research on effect of fetuin A in the occurrence and development of non-alcoholic fatty liver disease.

To explore the serum hepatocyte growth factor (HGF) lesion severity level and diabetic nephropathy (DN).A total of 207 patients with type 2 diabetes mellitus (T2DM) were recruited from Second Municipal Hospital.According to the level of urinary albumin excretion rate (UAER), they were divided into non-DN group (group A, n =56) : UAER ＜ 20 μg/min;early DN (group B, n =55) : UAER: 20-200 μg/min;clinical stage DN with normal renal function (group C, n =52):UAER ＞ 200 μg/min;DN in clinical renal dysfunction (group D, n =44) : UAER ＞ 200 μmol/min, decompensated chronic renal failure (serum creatinine: 133-177 μmol/L).And 55 healthy subjects were selected as controls.The serum levels of HGF, fasting plasma glucose , plasma glucose at 2 hour , blood urea nitrogen, creatinine and glycosylated hemoglobin were determined.HGF value : control group : (256.4 ± 30.4) ng/L,group A: (400.1 ±39.3)ng/L, group B: (689.4 ±55.6) ng/L, group C:(967.3 ±68.5) ng/L and group D: (150.7 ±23.2) ng/L.There are differences of serum HGF in different degree of DN.