Objective:To study the clinical value of susceptibility weighted imaging(SWI)in brain diseases.Methods:37 cases with blood vessel pathological change of brain diseases were collected and analyzed,including 8 example of angioma cavernosum,10 cerebral infarction,15 brain tumor,2 venous malformation and 2 venous sinus thrombus.Conventional T1WI,T2WI,DWI,the SWI and enhanced T1WI,MRA images were performed to compare the superiority of the SWI images to the others in demonstrating the small hemorrhagic focuses,the small vein,hemosiderin,calcification and other paramagnetic materials.Results:SWI could distinguish the hemorrhage from the blood vessel and discover the smaller hemorrhagic focuses of the angioma cavernosum.It could discover more tiny veins adjacent to the dilated draining vein.SWI could detect small hemorrhages in acute cerebral infarction and small draining veins in brain tumor.And draining veins in deep part and expanded rete venosum in the surfaces of brains could be obviously demonstrated when sinus venosus thrombus occurred.Conclusion:SWI is a new pulse sequence that can demonstrate the abnormal low current capacity blood vessels,the structure of small vein,the tiny hemorrhages as well as ferrugination and calcium deposition sensitively.So it can be applied in the diagnosis and antidiastole of the central nervous system diseases as an important supplement to the MRI conventional sequences.

10 kinds of normal and immunodifi-cient mice were used and they were found to have different levels ofNK activity.The NK activity toHuman to Human,Human to Mouseand Mouse to Mouse hybridomacells was much lower thanthat to YAC-1 cells in all mice andthere were no significant differencesbetween the two kinds of human Bcell hybridomas in susceptibility toNK cells.The results showed thatNK cells had no obvious effects onxenografts of human B cell hybri-domas.

The red blood cell sorbitol levels of 23 healthy subjects and 66 diabetic patients were measured enzymatically. Results showed that erythrocyte sorbitol levels of both IDDM (17) and NIDDM (38) patients with poor control were significantly higher than that of the controls and positively correlated with the coincident plasma glucose concentration, 24-hour urinary glucose excretion and HbA1c. There was no significant difference between the red cell sorbitol concentration of NIDDM with normal blood glucose level and that of the normal group; moreover, red cell sorbitol levels did not sig-nificantly correlated with FBG in both groups. Although there was no significant difference between the FBG of poorly controlled IDDM and NIDDM groups, red blood cell sorbitol levels were significantly different in both groups. This suggested that red blood cell sorbitol level could be used as an indicator of diabetic control.

The present paper is an analysis of the pattern reversal visual evoked potentials (P-VEP) of macular disease (40 cases, 57 eyes), in contrast to normal persons (40 cases, 80 eyes) of similar age. The group consists of 9 different kinds of disease-central serous chorioretinopathy macular hemorrhage, macular dege-neration, etc. The authors analyzed the latency and amplitude of P-VEP in different macular disease with different course and central vision. The results showed that the latency of P-VEP in the group was relatively prolonged. In contrast to normal person P 100 was prolonged by 9.0, 26, 40 ms at 1?2′, 30′, 16′ visual angles respectively. The amplitude was obviously lowered, especially at the visual angle of 16′ and P-VEP extinguished in all the eases with visual acuity less than 0.1.The latency and the amp-litude were not related to the type and the course of the maeular disease, but were closely related to the central vision.

Objective To study the effect of acid burn on rat corneal endothelial damage and its repair.Methods The model of mild,moderate and severe acid burn in cornea was established in 90 Long-Evans rats(n=30 for each degree of acid burn) with 4 rats served as normal controls.Corneal edema,opacity and the healing of epithelium were observed with a slit lamp microscope.The corneal endothelium from half rats of each degree acid burn was stained with trypan blue and alizarin red,and that from the other half with hematoxylin and eosin(HE) immediately,30 min,1,3,7,14,21,28 d after cautery.The endothelial damage and its repair were observed and the number of the leucocytes in cornea was calculated under light microscope.Results No damage was seen in the corneal endothelial cells of mild,moderate acid burn,only a few leucocytes could be seen in the cornea,and the healing of epithelium,the regression of edema were within 7 d.The endothelial cells necrosed and shedded in the wound area by severe acid burn.The endothelial cells in the margin were deformed,elongated and slided to the damaged area during repair,and a great number of leucocytes infiltrated,the healing of corneal epithelium was delayed,the regression of edema was up to 28 d.Conclusion The corneal endothelial cells were damaged in severe acid burn.The secondary inflammatory reaction took part in the damage of corneal endothelial cells after severe acid burn.The damage of endothelial cells delayed the healing of corneal ulcer and edema.

Objective: To investigate the effect of two modes of one-lung ventilation(OLV) on lung lobectomy in pediatric patients.Metheds: Twenty ASA Ⅰ-Ⅱ patients(8 male,12 female) aged 2~14 year,weighing 10~30 kg,and undergoing lung lobectomy were enrolled in this study.The patients were premedicated with intramuscular Sod Luminal 3~5 mg/kg and Atropine 0.01 mg/kg im,30 minutes before induction.Induction was performed with Dorcum 0.1 mg/kg,Fentanyl 5 ?g/kg and Vecuronium 0.1 mg/kg,maintained with Vecuronium 70 ?g/(kg?h) and Propofol 5mg/(kg?h) iv boluses.Ordinary tracheal tube was inserted into bronchus(14right,6 left) during OLV.First,OLV with volume-control mode maintained 25 min,and then,OLV with pressure-control mode was performed.In first step Vt was set at 8~10 ml/kg and respiratory rate was adjusted to maintain PetCO2 between 30~40 mmHg.In second step the inspiratory pressure was set according to the plateau pressure during step 1,hemodynamics and PaO2 were measured and recorded.Results : During OLV with pressure-control mode PaO2 was significantly increased,as compared with that during volume-control mode(P

Objective: To study the expression of Echinococcus multilocularis 14-3-3 antigen encoding gene in Bacillus Calmette-Guerin(BCG).Methods: The recombinant plasmid pBCG-Em14-3-3 was introduced into BCG by electroporation and the bacteria with pBCG-Em14-3-3 were cultured to logarithm growth period,then induced daily by heating at 45℃ for 30min in the last three days.The expression products were analyzed by SDS-PAGE and Western blot.Results: The Em14-3-3 recombinant protein was successfully expressed by pBCG-Em14-3-3 in BCG as an obvious band of 27kDa by SDS-PAGE,which accounted for 11% of total bacterial protein in BCG.Conclusion: The pBCG-Em14-3-3 could be expressed efficiently in BCG and be combined with specific antibody in mice sera,which suggested that the Em14-3-3 recombinant protein expressed by rBCG-Em14-3-3 vaccine was of specific antigenicity.

Objective:To summarize the anesthesia experience of open heart surgery in 43 infants(weigh less than 5kg) and to explore the related issues with myocardioprotection and brain protection.Methods:Conventional induction of anesthesia,nasotracheal intubation,arteriovenous puncture,cardiopulmonary bypass were established,according to the needs for maintaining the body low temperature(deep,middle,superficial);haemodynamics was maintained with vasoactive agent after aorta was opened.Results:Two infants with transposition of conducting arteries succumbed to stoma leak.One infant with tetralogy of Fallot succumbed to low cardiac output syndrome,bellows complication appeared in 8 cases,Ⅲ.atrial ventricular block 1,and the rest infants recovered well.Mortality was 6.98%in this group.Conclusion:Cardiac anesthesia control in infants with low weight is difficult,death rate is hiqh and complication is complex.Butas long as control is done well,mortality omd complitation can be devreased.

Objective:To study the status quo of quality of life and its influencing factors among Junior High School Students of Three-Gorges Reservoir and Non-Three-Gorges Reservoir in Chongqing,and to provide basis for students' QOL improvement.Methods:672 Three-Gorges Reservoir students and 630 Non-Three-Gorges Reservoir students were tested by using the Quality of Life Scale for Junior High School Students(JHSS-QOLS).Multiple stepwise regression analysis was utilized to study the major factors affecting QOL of students in Chongqing.Results:The quality of life of junior high school students in Three-Gorges Reservoir was higher than that of students in Non-Three-Gorges Reservoir.Physical exercises,sleep,chronic disease,parental rearing style were important influencing factors in quality of life of students.Conclusions:The key steps of improving the quality of life of Junior High School Students should include reducing the learning load of students,the prevention and control of chronic disease and the improvement of parental educational level.

Artificial hip replacement is an effective therapy to eliminate hip pain,correct deformity and restore function.With population aging and improving of people life quality,artificial hip replacement has been widely conducted.Over the past 40 years,artificial hip replacement technique has rapidly developed,and minimally invasive technology presents development direction of future artificial hip replacement.Skilled operation experience and instrument application are two key factors to ensure surgery success.The minimally invasive technology arouses more attention from clinical doctors and patients due to minimal invasion,little blood loss and fast recovery.However,not all patients can undergo minimally invasive hip replacement.This paper summarized related studies on minimally invasive hip replacement in domestic,and discussed the indications,surgical approach,complications and prevention.