1.Pharmacological intervention for contact granuloma of the larynx.
Jin-Rang LI ; Hong-Guang GUO ; Xi CHEN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2008;43(5):387-388
Adult
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Aged
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Aged, 80 and over
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Granuloma
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drug therapy
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Humans
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Laryngeal Diseases
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drug therapy
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Male
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Middle Aged
2.Studies on twelve cytokines in serum of coronary heart disease patients
Xing-Wang JIA ; Ya-Ping TIAN ; Guang-Hong GUO ;
Chinese Journal of Laboratory Medicine 2003;0(09):-
Objective The significance of serum cytokine profile in coronary heart disease patients have been evaluated by observing the variation cytokines Methods The serum IL-1 a,IL-1 ?,IL-2,IL-4, IL-6 ,IL-8,IL-10,VEGF,IFN-?,,TNF-?,MCP-1 and EGF in 76 patients and 26 matched healthy volunteers have been observed.Serum cytokines had been measured by protein chips methods in EVIDENCE 180 automatic biochips analyzer and cytokine profile were got by bioinformatics.Results The serum level of IL-1?,IL-6,IL-8,and TNF-? were (4.32?8.14) ng/L ,(13.16?16.81) rig/L,(375.53?292.14) ng/L,(15.46?15.38) ng/L respectively,which increased obviously in patients (P
3.Application of Scaffolds in Construction of Random Peptide Libraries and Targeted Molecules Selection
Hong-Guang GUO ; Hai-Jiang WU ; Can-Quan MAO ;
China Biotechnology 2006;0(09):-
Nature is abundant in protein scaffolds.By selecting suitable protein scaffold,display and screening methods,the rational and constrained random peptide library(RPL)can be constructed.Compared with the non-constrained RPL,it offered more opportunities for obtaining novel protein structures and more higher affinity ligands against the target molecules.At present,the protein scaffold constrained RPLs have been shown great potential in applications such as target selection,basic research,clinical diagnosis,medical therapy and so on.It is systematically introduced the structure bases,classification and construction of constrained RPL based on scaffolds,as well the recent great advances of application in selection against target molecules with S-S constrained scaffolds,antibodies,Zinc finger protein,Z domain,FN3 domain as important examples.
4.Epithelioid angiosarcoma after radical mastectomy for breast carcinoma: a case report.
Hong-ji DING ; Guo-guang WEI ; Li-qun YU ; Qi-song WU ; Yan-guang DONG
Chinese Journal of Pathology 2006;35(10):637-638
Aged
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Antigens, CD34
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metabolism
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Breast Neoplasms
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metabolism
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pathology
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therapy
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Chemotherapy, Adjuvant
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Combined Modality Therapy
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Epithelioid Cells
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pathology
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Female
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Follow-Up Studies
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Hemangiosarcoma
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metabolism
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pathology
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Humans
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Immunohistochemistry
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Mastectomy, Radical
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Neoplasms, Second Primary
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metabolism
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pathology
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Radiotherapy, Adjuvant
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Vimentin
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metabolism
5.Construction of the Expression Vector of Viruslike Particles Containing FMDV IRES RNA
Min DOU ; GuoGuang ZHANG ; GuangFu YU ; HongXin ZHANG ; MingShan SHEN ; Liang CHEN ;
China Biotechnology 2006;0(09):-
The Coat protein and Maturase gene of E.coli bacteriophage MS2 was amplified by PCR,then the gene was cloned into pET32a to construct the intermediate vector pET32aCP.The conservative sequence of FMDV internal ribosome entry site(IRES) was cloned into the downstream of pET32aCP bacteriophage gene to construct the prokaryotic expression vector pCPES.The recombinant plasmid pCPES transformed into E.coli strain BL21(DE3) was induced to express with 1mmol/L IPTG.The expression products were purified by sucrose density gradient centrifugation.The expression products observed by TEM were circular viruslike particles,and the diameter of these particles was about 26nm.The stability of viruslike particles was detected,and the viruslike particles was identified by RTPCR.The results showed that the viruslike particles contain the FMDV IRES RNA and have good stability.The viruslike particles have great prospect as the standard and quality control in the area of RNA virus detection.
6.Study on Rapid Methods for Quantitative Analysis Rhamnolipid and Its Influence Factors
Guo-Man LU ; Hong-Yu LIU ; Guang-Ming ZENG ; Guo-He HUANG ; Hui ZHANG ;
Microbiology 1992;0(04):-
In order to find an easy and rapid quantitative analytical method to detect rhamnolipid produced by Pseudomonas aeruginosa, three methods, H_ 2 SO_ 4 -anthrone analysis method, L-cysteine-H_ 2 SO_ 4 method and phenol-H_ 2 SO_ 4 method, were compared in the present paper, and the influence factors were also considered.The results showed that H_ 2 SO_ 4 -Anthrone analysis method was better than the others and its optimal reaction condition was obtained.The influence to the quantitative analysis of rhamnolipid from the residual glucose and the top clean liquid layer in the ferment solution could be ignored.But the influence from the bacterial body and the middle layer of the ferment solution reached a certain degree.Thus, the bacterial body should be removed before measuring.However, the influence from the middle layer of the ferment solution could be avoided by making a standard curve which was made by using a rhamnose mixed with the middle layer ferment solution.
7.Determination of clonidine in rabbit plasma by HPLC-MS.
Guang-ming KE ; En-hong ZHANG ; Li WANG ; Qiang ZHANG ; Hong-guang DU ; Hong-you GUO
Acta Pharmaceutica Sinica 2004;39(5):367-369
AIMTo determine clonidine in rabbit plasma by LC-MS.
METHODSThe LC-MS system consisted of Waters Alliance 2790 HPLC and Micromass ZQ-4000 MS. The HPLC was performed by using XTerra C18 (150 mm x 2.1 mm ID, 5 microm). The mobile phase, consisting of acetonitrile/ammonium hydrogen carbonate solution, was maintained to a flow-rate of 0.2 mL x min(-1) and the linear gradient elution was adopted. Mass spectrum was obtained by using electrospray ionization interface and the m/z of SIM was 230.
RESULTSThe average recovery was high and the method was reproducible. The calibration curve showed good linearity in the range of 1 - 80 microg x L(-1), the lowest limit of detection was 0.05 microg x L(-1). The Cmax, AUC0-t, and Tmax value of the pharmacokinetics parameter were (27 +/- 9) microg x L(-1), (5,352 +/- 1,121) microg x L(-1), (79 +/- 17) h.
CONCLUSIONThe results demonstrated that the method had high sensitivity, good selectivity, accuracy and precision. It is used to determine the clonidine concentration in plasma. The transdermal patch can deliver clonidine to the surface of rabbit skin stably for periods of up to 1 week after a single application.
Administration, Cutaneous ; Animals ; Antihypertensive Agents ; administration & dosage ; blood ; pharmacokinetics ; Area Under Curve ; Chromatography, High Pressure Liquid ; methods ; Clonidine ; administration & dosage ; blood ; pharmacokinetics ; Rabbits ; Spectrometry, Mass, Electrospray Ionization ; methods
8.Prudent use price controls in Chinese medicines market: based on statistical data analysis.
Guang YANG ; Nuo WANG ; Lu-Qi HUANG ; Hong-Yan QIU ; Lan-Ping GUO
China Journal of Chinese Materia Medica 2014;39(1):144-148
A dispute about the decreasing-price problem of traditional Chinese medicine (TCM) has recently arisen. This article analyzes the statistical data of 1995-2011 in China, the results showed that the main responsibility of expensive health care has no direct relationship with the drug price. The price index of TCM rose significantly slower than the medicine prices, the production margins of TCM affected by the material prices has been diminishing since 1995, continuous price reduction will further depress profits of the TCM industry. Considering the pros and cons of raw materials vary greatly in price, decreasing medicine price behavior will force enterprises to use inferior materials in order to maintain corporate profits. The results have the guiding meaning to medicine price management.
Commerce
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economics
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Data Interpretation, Statistical
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Health Care Sector
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economics
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Humans
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Medicine, Chinese Traditional
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economics
9.Application of "disharmony of stomach leads to insomnia" theory in acupuncture treatment for insomnia.
Wen-Li WANG ; Jian-Wu LIU ; Li-Guo CHEN ; Chen-Guang WANG ; Yan-Hong ZHANG
Chinese Acupuncture & Moxibustion 2014;34(12):1228-1230
When looking back to the literature regarding insomnia, it is believed that "disharmony of stomach leads to insomnia" is an important mechanism for the attack of insomnia. This theory is widely accepted in clinical practices by ancient and modern acupuncturists, thus the method treating insomnia from the stomach is developed. But deeper research on its mechanism is still lacking. Through correlation between stomach meridian and heart meridian, spleen and stomach being the pivot of rise and decline of qi movement and yin-yang and being the pivot of five zang-viscera housing spirit, modern abdomen-brain theory and experimental research, the mechanism of treating insomnia from the stomach is discussed in this paper, so as to be better to guide clinical acupoint-selection and treatment.
Acupuncture Therapy
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history
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China
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History, Ancient
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Humans
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Medicine in Literature
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Sleep Initiation and Maintenance Disorders
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physiopathology
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therapy
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Stomach
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physiopathology
10.The significance of galectin-3 and matrix metalloproteinase-2 expression in patients with glioma
Guihua GUO ; Yajuan ZHUANG ; Hong QIU ; Yunchao LI ; Guang CHEN ; Xiangdong YU ; Yujue WANG
Clinical Medicine of China 2014;(7):735-738
Objective To investigate the significance of galactose galectin-3(Galectin-3)and matrix metalloproteinase-2(MMP-2)expression in gliomas patients and thire role in process of gliomas' malignancy development. Methods Immunohistochemistry was used to detect Galectin-3 and MMP-2 protein expression in 5 normal brain tissue and 40 patients with different grade gliomas. According to positive cells number of Galectin-3,MMP-2 in tumor cells under a microscope,to determine the expression,and the positive index(LI)which came from the percentage of the positive cell number out of the total cell number was to expressed the number of positive cell. Results Galectin-3 and MMP-2 protein expression in normal brain tissue were negative. In glioma tissues,Galectin-3 was mainly expressed in the cytoplasm and membrane of tumor cells. In 23 glioma tissue withⅠor Ⅱ grade,9 cases(39. 13% )was positive and the LI values was(5. 65 ± 3. 47)% in terms of Galectin-3 expression. In Ⅲ,Ⅳ grade glioma specimens,the positive rate of Galectin-3 expression was 76. 47%(13 / 17), and LI value was(27. 88 ± 22. 13)% . The difference of Galectin-3 expression and LI value were significant between specimens with Ⅰ,Ⅱ grade and Ⅲ,Ⅳ significant( χ2 = 4. 101,t = 4. 105;P < 0. 05). In human gliomas,MMP-2 expression protein was mainly expressed in tumor cells and vascular basement membrane of the endothelial cell cytoplasm. In 23 glioma tissue with Ⅰor Ⅱ grade,9 cases(39. 13% )was positive and the LI values was(5. 91 ± 4. 78)% in terms of MMP-2 expression. In Ⅲ,Ⅳ grade glioma specimens,the positive rate of MMP-2 expression was 88. 24%(15 / 17),and LI value was(30. 06 ± 22. 94)% . The difference of MMP-2 expression was significant between specimens with Ⅰ,Ⅱ grade or Ⅲ,Ⅳ grade( χ2 = 7. 882,t = 4. 271;P< 0. 05). The linear correlation analysis showed that there was positively correlation between Galectin-3 and MMP-2 positive cells(r = 0. 800,P < 0. 05). Conclusion Galectin-3 and MMP-2 protein expression in Ⅰ,Ⅱgrade gliomas is significantly lower than those inⅢ,Ⅳ grade glioma,and they are positively related with the progress of malignant gliomas. Galectin-3 and MMP-2 protein can be used to evaluate or judge the malignant stage of human brain glioma.