OBJECTIVE:To optimize the extraction conditions of volatile oil from Chrysanthemum indicum and the processing techniques of its ?-yclodextrin(?-CD) inclusion compound.METHODS:The extraction conditions of volatile oil from Chrysanthemum indicum was optimized taking soaking time,ultrasonic treatment time,water amount as influencing factors and with the yield of the volatile oil as index.The processing techniques of the ?-yclodextrin (?-CD) inclusion compound was optimized taking the ratio of ?-CD to volatile oil,the time and temperature of the inclusion process as indexes and with the yield of the inclusion compound and the inclusion rate of the volatile oil as indexes. RESULTS:The optimal process for oil extraction was as follows:the soaking time=1.5 h,the ultrasonic treatment time= 40 min,heating time=6 h,water amount=600 mL.The optimal processing conditions for(?-CD) inclusion compound of Chrysanthemum indicum were as follows: the ratio of ?-CD to volatile oil=8∶1,the inclusion time of the compound was 2 hours at 40 ℃.CONCLUSION:High extraction yield of vlatile oil and stable inclusion compound were obtained from the above method.

Objective To investigate the quality of marriage and sexual function of cervical cancer patients following radical hysterectomy and vaginal extension.Methods Case-control and questionnairebased methods were used in this study.Forty patients of early-stage (Ⅰ b 1 ～ Ⅰ b2) cervical cancer who had undergone vaginal extension following classic radical hysterectomy were included in the observation group,while 40 patients with matching factors and radical hysterectomy only during the same period were included in the control group.The quality of marriage and sexual function were compared with OLSON marriage prolapse and incontinence sexual function questionnaire Shon Fonn (PISQ-12).Results The operation time and postoperative vaginal length in the observation group were higher than those in the control group (P ＜ 0.05 or P ＜ 0.01).The emotional,physical,and total scores during postoperation were lower than those during preoperation (P ＜ 0.01).The emotional,physical,and total scores in the control group were lower than those in the observation group (P ＜ 0.01).The marital satisfaction,husband and wife communication,conflict resolution methods,extracurricular activities and sex scores in the observation group were higher than those in the control group (P ＜ 0.05 or P ＜ 0.01).Conclusions Patients with peritoneovaginoplasty following radical hysterectomy had much longer vagina and less self-perceived short vagina.Vaginal extension following radical hysterectomy does not worsen the pelvic floor symptoms.

Most neonatal diabetes mellitus (NDM) is caused by genetic abnormality. Sulfonylurea (SU) has been successfully applied in NDM patients;The pathogenesis of NDM and mechanism of sulfonylurea on molecular level have been illuminated, and recent studies revealed that glycated albumin is a useful glycemic indicator. In this review, the research progress of NDM is summarized.

Objective To investigate the effect of transfusion of allogeneic apoptotic splenocytes on immunological tolerance to pancreatic islets transplantation in rat. Method Recipient SD rats were rendered diabetic mellitus by a single intraperitoneal injection of streptozotocin (STZ). The apoptosis of donor Wistar rat splenocytes was induced by X-irradiation with electron linear accelerator. Pancreatic islets were isolated by intraductal collagenase P, and were purified by Ficoll-400 discontinuous density gradient after culturing for 1-2 days from donor Wistar rat. The diabetic SD rats were randomly divided into 4 groups, and respectively received infusion of Hanks solution, donor normal splenocytes, donor apoptotic splenocytes, and donor necrotic splenocytes via dorsal veins of penis, and then they received pancreatic islets from the same donor rat underneath their renal subcapsular space 7 days later. Mixed lymphocyte reaction (MLR) was used as immunological parameter of transplantation tolerance besides the observation of blood glucose level and allograft survival time. Islet graft failure was defined as the presence of a plasma glucose concentration of at least 11.0mmol/L on 2 successive days. Results The pre-infusion of donor apoptotic splenocytes significantly prolonged the survival time of allograft as shown by the median survival time (MST) of graft of 31days, and the longest survival time of 42 day, and it apparently decreased the rejection response in recipient SD rat in one-way mixed lymphocyte culture. Conclusion Transfusion of donor apoptotic splenocytes could induce immunological tolerance of allogenic islets transplantation in rat.

Objective To determine the planning target volume margins of head and neck cancers treated by image guided radiotherapy (IGRT).Methods 464 sets cone beam computed tomography (CBCT) images before setup correction and 126 sets CBCT images after correction were obtained from 51 head and neck cancer patients treated by IGRT in our department.The systematic and random errors were evaluated by either online or offline correction through registering the CBCT images to the planning CT.The data was divided into 3 groups according to the online correction times.Results The isocenter shift were 0.37 mm±2.37 mm, -0.43 mm±2.30 mm and 0.47 mm±2.65 mm in right-left (RL), anterior-posterior (AP) and superior-inferior (SI) directions respectively before correction, and it reduced to 0.08 mm±0.68 mm, -0.03 mm±0.74 mm and 0.03 mm±0.80 mm when evaluated by 126 sets corrected CBCT images.The planning target volume (PTV) margin from clinical target volume (CTV) before correction were:6.41 mm,6.15 mm and 7.10 mm based on two parameter model, and it reduced to 1.78 mm,1.80 mm and 1.97 mm after correction.The PTV margins were 3.8 mm,3.8 mm,4.0 mm;4.0 mm,4.0 mm,5.0 mm and 5.4 mm,5.2 mm,6.1 mm in RL, AP and SI respectively when online-correction times were more than 15 times, 11-15 times,5-10 times.Conclusions CBCT-based on online correction reduce the PTV margin for head and neck cancers treated by IGRT and ensure more precise dose delivery and less normal tissue complications.

BACKGROUND:The large watercress molded piece is longer than that of the normal molded piece, which is suitable for dental restoration of elderly patients with gingival recession and relatively long crown.
OBJECTIVE:To test and verify the effect of large watercress molded piece on the auxiliary repair of approximal caries lesions in molars of the elderly.
METHODS:Eighty-seven senile patients with molar approximal caries were randomly divided into two groups: those were repaired by large watercress molded piece as experimental group, and those repaired by traditional amalgam molded piece ascontrol group. Then we compared the comfortable satisfaction rate, the excelent and good rate of repair and the gingival index between the two groups immediately, 1, 3, 6 and 12 months after repair.
RESULTS AND CONCLUSION:The comfortable satisfaction rate of the experimental group was significantly higher than that of the control group at 1, 3, 6 and 12 months after repair (P< 0.05), and the gingival index was significantly lower than that of the control group (P< 0.05). The excelent and good rate of the experimental group was significantly higher than that of the control group at different time points after repair (P< 0.05). No loss or fracture of the restorations occurred in the two groups. These results demonstrate that the large watercress molded piece for repairing approximal caries lesions in molars of the elderly can better restore the anatomical shape and approximal points of teeth, and reduce food impaction and gingival inflammation.

Objective To investigate the clinical efficacy of mini-plate fixation for ulnar coronoid process fracture via anteromedial approach.Methods A retrospective review was made on 28 cases of ulnar coronoid process fracture treated with open reduction and mini-plate fixation from April 2010 to December 2014.There were 18 males and 10 females,with age range of 21-60 years (mean,34.3 years).Causes of injury were falls in 21 cases and traffic accidents in 7 cases.According to the O' Driscoll classification,there were 14 cases of type Ⅰ,11 cases of type Ⅱ and 3 cases of type Ⅲ.Time from injury to operation was 2-10 d (mean,3.7 d).Reduction loss,fracture healing time and complications were documented.Elbow function was evaluated at the last follow-up by Mayo elbow performance score (MEPS) and Broberg & Morrey score.Results All cases were followed up for 12-25 months (mean,15.1 months).Restricted elbow activity was initiated one week after operation.Bone united in mean 10.6 weeks (range,8-14 weeks).No nerve injury or fracture redisplacement occurred.At the final follow-up,mean flexion was (124 ± 15)° (range,90°-140°),mean extension loss was (18 ± 20) °(range,0°-50°),mean pronation was (65 ± 18)°(range,40°-85°),and mean supination was (63 ±16)°(range,35°-85°),showing significant difference in comparison with the preoperative measure (P＜0.05).According to the MEPS,the results were excellent in 17 cases,good in 8,fair in 2 and poor in 1.According to the Broberg & Morrey score,the results were excellent in 16 cases,good in 8,fair in 3 and poor in 1.Conclusion Through anteromedial approach,mini-plate fixation of the ulnar coronoid process fracture provides rigid fixation,which benefits joint stability and bone union,and allows early functional exercise.

Objective To study the role of nicorandil in protecting myocardium of very old patients with non-ST segment elevation myocardial infarction.Methods Ninety-six very old patients with non-ST segment elevation myocardial infarction admitted to our hospital from March 2012 to July 2016 were randomly divided into experimental group (n=48) and control group (n=48).After 4 weeks of comprehensive treatment,the patients in experimental group were given oral nicorandil (5 mg,3 times a day).The serum cTnI level was measured in 2 groups before treatment and on days 1,3,7 and 10 after treatment.The ECG of 2 groups was observed before and after treatment.Results The serum cTnI level was significantly lower in experimental group than in control group on days 3,7 and 10 after treatment (3.24±4.31 μg/L vs 10.47±t4.72 μg/L,P＜0.05;0.53±0.31 μg/L vs 2.50±1.45 μg/L,P＜0.01;0.09±0.07 μg/L vs O.41±0.26 μg/L,P＜0.01).The ECG was significantly better in experimental group than in control group after treatment (P＜0.05).Conclution Oral nicorandil can protect the myocardium of very old patients with non-ST segment elevation myocardial infarction.