Consensus Development Conferences as Topic ; Endoscopy ; Europe ; Humans ; Nose Neoplasms ; diagnosis ; surgery ; Skull Base Neoplasms ; diagnosis ; surgery

Diagnosis, Differential ; Humans ; Medicine, Chinese Traditional

Perforator stroke(PS)is one of peri-procedural complications associated with stenting of intracranial arterial stenosis.A lot of perforator arteries are derived from intracranial arteries with difference from coronary arteries,because once the intracranial perforator arteries of crucial regions are occluded,may lead to fatal prognosis.Three hypothesis have been used to explain PS including snow-plowing,stent jailing effect and in-stent neointimal growth.In vivo models,investigators have already found these phenomenas.After much attention been paid on PS,authors have published a report covered with a large amount of cases in 2006. The author figured out that patients with perforator infarct adjacent to the stenotie segment(PIAS)under MRI beforehand would have higher chance occurring PS after stenting of intracranial stenosis.The patients with priority of PS easily are affected by the morphology,location,etiology,sites,the type and equipments.Many yet unsolved problems including the prevention would arouse us to go deeper into for the exploration of the mechanism.(J Intervent Radiol,2007,16:858-862)

0.05].After 12 week treatment,the YGTSS total score in study group was significantly lower [(27.88?14.24) vs(36.53?17.45) scores] and the reduction rate of YGTSS score was significantly higher [(43.12?18.42)% vs(33.49?14.38)%] compared with those in control group(Pa

Universities are cradles of leadership and full-developed specific professional.Besides specific courses,reasonable sexual health education should be set up to make the students succeed when they grow up.

Based on the gene sequence of AIV matrix protein 2(M2) and two cytotoxic T-lymphocyte epitopes derived from nucleoprotein,the prokaryotic expression vector pET-3M2e-NP1-NP2 was constructed.The target gene was expressed in the solvable form in BL21(DE3) when induced with 1.0 mmol/L IPTG and Western blot analysis showed that the expression product had good immunogencity.Purified fusion protein was mixed with various amount of adjuvant,including Freund's,Vash oil and chitosan,and then immunized 20-day-old chicken by intramuscular injection and boosted 3weeks later.Blood samples were collected weekly following the primary vaccination.The anti-M2e antibody was detected with ELISA method with the synthesized peptide as coated antigen;the neutralizing ability of anti-serum was evaluated on MDCK cell line and chick embryo,the CD4+ and CD8+ T lymphocyte amounts in peripheral blood of immunized chicken was measured by flow cytometry.Results showed that the fusion protein can induce immunological reaction,and the antibody can bind with the viral M2 protein that expressed on the surface of MDCK cells.Flow cytometry result showed that CD4+ and CD8+ T lymphocyte in peripheral blood increased obviously following immunization(P

M2e gene of three copies for H5N1 subtype AIV was synthesized and fused with human mycobacterium tuberculosis hsp70 gene.The fused gene was cloned into the prokaryotic expression vector to get pET-3M2e and pET-3M2e-hsp70.Recombinant protein r3M2e and r3M2e-hsp70 were successfully expressed induced with IPTG and purified with Ni2+-NTA collumn.Following that,the immunity of the recombinant protein was analysized with Western blot.20-day-old AIV non-immunized chickens were vaccined with r3M2e and r3M2e hsp70,at the same time,Trx and KLH-M2e inoculated chickens were served as vector and positive controls.Two weeks after the primary vaccination,every group was boosted with the same vaccine as in the primary vaccination.The humoral immunity of the vaccined chickens was evaluated with antibody detection against M2e,cytopathic suppression test,and indirect fluorescence assay.The cellular immunity was estimated according to lymphocyte subtype analysis with flow cytometry and M2e specific cytokine detection.Four weeks after the boost vaccine,all groups were challenged with 100EID50 AIV of H9N2 subtype,and the virus from swabs was detected with Real-time PCR.Results indicated that r3M2e hsp70 vaccined chicken developed the better humoral and cellular immune response,also,made a better performance compared with r3M2e vaccined group in virus challenge.

Objective To build the experimental basement for the clinical use of cytokines induced killer(CIK)cells from the cord blood mononuclear cells(CBMNC)in tumor adoptive cellular immunotherapy, an effective protocol for their proliferation in vitro and cytotoxicity of CIK cells was established.Methods The lymphocytes from umbilical cord blood were isolated by density gradient centrifugation and suspended in medium with CD_3 mAb,rIL-2,rIL-1 and IFN-? as inducing agents to prepare CIK cells.At the same time, the lymphokine activated killer(LAK)and CBMNC were set as controls,which were only added IL-2 and not any cytokines during the whole culture.The changes of CIK cells before and after induction were observed with microscope and the phenotypes of the cells were analyzed by using flow cytometry.The proliferation of CIK cells were determined by trypan blue exclusion assay and the cytotoxic activity to lung cancer cell were tested with MTF method.Results According to the experiment,combining use of four types of cytokines could generate a great deal of CIK cells possessing highly cytotoxicity.From day 5 CIK cells became to prolif- erate and reached the peak at day 14.During the whole period,the relative percentage of CD_3~+ CD_(56)~+ cells in- creased significantly.Compared with LAK cells,which reached the proliferation peak at day 7 and then showed no evident proliferation.The control cells(CBMNC)showed no evident change of phenotypes and proliferation.CIK cells showed a higher antitumor activity on the tumor cells than LAK cells and CBMNC in vitro.Conclusion Umbilical cord blood can generate a great deal of CIK cells combining used with cy- tokines.Compared with classic LAK cells,umbilical cord blood CIK cells have the advantages of rapid prolif- eration speed and powerful cytotoxicity.CIK cells will be promising as a new strategy for the adoptive cellular immunotherapy of tumor.

Objective To discuss the application of prospective risk nursing measures in reducing the occurrence of postoperative local hematoma at femoral artery puncture site. Methods A total of 605 patients receiving femoral artery puncture for interventional management were collected as the control group , and 322 patients were used as the intervention group. The risk factors causing postoperative local hematoma at femoral artery puncture site in the control group were recorded, and based on which risk estimation survey table was designed. According to risk estimation survey, the prospective intervention measures, including risk assessment, standardization of professional training, individual health education, the improvement of care appliance, etc. were carried out for the patients of the intervention group. The occurrences of postoperative local hematoma at femoral artery puncture site were recorded and the results were compared between the two groups. Results No statistically significant differences in demographic and clinical data existed between the two groups. However, the incidence of hematoma in the intervention group was significantly lower than that in the control group (χ2 = 4.652, P < 0.05), although the difference in the severity of hematoma was not significant between the two groups. Conclusion The use of prospective risk nursing measures can effectively reduce the incidence of postoperative hematoma at femoral artery puncture site.

Objective Adsorption effect of dietary fibers from seaweeds on extrinsic estrogens was studied.Methods The methods included the tests in vivo and in vitro which simulated environments of stomach and small intestine of rat. Results The adsorption effect of four kinds of dietary fibers on estradiol in simulated environments of small intestine was better than that in simulated environments of stomach. The effect of Laminaria japonica was the best. And the rate of adsorption on estradiol was 108 g?g~ -1.It is 1.5,1.8, 3.1, and 3.0 times that of Sargassum,wheat blan,Gracilaria and Eucheuma,respectively. The rates of adsorption on estradiol of four kinds of dietary fibers from Gracilaria, Eucheuma, Sargassum and laminaria japonica was 11.1%,13.6%,27.5% and 38.7%, respectively. Adsorption rate of Sargassum and laminaria japonica was 1.9 and 1.3 times than that of wheat bran, respectiveiy which was 20.4%. While for Gracilaria and Eucheuma, adsorption rate was lower than that of wheat bran. Conclusion The dietary fibers from four seaweeds showed adsorption effect on extrinsic estrogens. The dietary fibers from brown seaweeds (Sargassum and laminaria japonica) had more adsorption effect than that of red seaweeds (Gracilaria and Eucheuma).