Humans ; Membrane Proteins ; metabolism ; Myelodysplastic Syndromes ; metabolism ; Receptors, Colony-Stimulating Factor ; metabolism ; Signal Transduction

In observing temperature changes in 100 cases of art ificial joint replacement,we discovered that the temperature curves p resented a “double peak” phenomenon.Accordingly,we analyzed the reasons for th is phenomenon and dealt with them,improving our nursing quality.

Adult ; Female ; Humans ; Lung Neoplasms ; complications ; microbiology ; Lymphoma ; complications ; microbiology ; Tuberculosis, Pulmonary ; complications

0.05). The level of serum IL-8 and expression of TNF ? in the colonic mucosa decreased at the 7th day ( P

Acupuncture Therapy ; Adult ; Aged ; Female ; Gastroesophageal Reflux ; therapy ; Humans ; Male ; Middle Aged ; Treatment Outcome

Dust ; Humans ; Occupational Exposure ; prevention & control ; Pneumoconiosis ; prevention & control

Adult ; Anemia, Hemolytic ; Female ; Humans

Objective To study the killing effect of focused ultrasound activated-hematoporphyrin on H-22 and its optimum exposure time.Methods The distribution of hematoporphyrin in H-22 cells was measured by a fluorescence photometer,the uhrastructure changes were evaluated at different time with a scanning electron micro- scope after treatment with focused ultrasound at the frequency of 1.43 MHz and intensity of 1.0 W/cm~2, 2.0 W/cm~2,3.0 W/cm~2,respectively,in combination with HpD.Results The concentration of HpD in the H-22 cells reached its peak after being added to H-22 tumor cells for 45 minutes,which will produce the hest anti-tumor effect when activated by ultrasound.Morphological observation showed that HpD alone had a slight influence on H-22 cells;ULtrasound alone showed an anti-tumor effect on tumor cells,which was dosage-dependent.Ultrasound dia- thermy in combination with HpD has more effective in terms of its antitumor effect when compared with uhrasound dia- thermy alone.Conclusion The killing effect on H-22 tumor cells of sonodynamic treatment was dependent on the intensity of uhrasound and the content of HpD in the cells,as well as on the time of action of both uhrasound and HpD.

Background Fractalkine is involved in the impairment of endothelium by mediating inflammatory cell chemotaxis.Aspirin inhibites many kinds of cytokine expression.Objective To investigate the effect of aspirin on tumor necrosis factor ?(TNF-?) stimulated fractalkine expression in human umbilical vein endothelial cells (HUVEC) and its mechanism.Methods HUVEC were grouped as follows:control group;TNF-?-stimu- lated;TNF-?+NS-398;TNF-?+PDTC and TNF plus various concentration of aspirin (0.02,0.2,1.5 mmol/L). The level of mRNA and protein expression of fractalkine and nuclear factor(NF)-kB p65 was determined by RT- PCR and Western blot.Results 1)Fractalkine mRNA and protein level was increased after 4 ?g/L TNF-? stim- ulation in HUVEC(both P

It is important to pay more attention to students’ basic skills training and their comprehensive abilities cultivating in microbiology experiment teaching. Explorations and reforms in enforcing the students’ base and cultivating their abilities were carried out in order to improve teaching quality and train specialized talents with high quality.