Animals ; Avoidance Learning ; physiology ; Cues ; Extinction, Psychological ; physiology ; Fear ; physiology ; Male ; Mental Recall ; physiology ; Rats ; Sleep Deprivation ; physiopathology ; Sleep, REM ; physiology

This study is to establish the gastric hot model of rats. After gastric feeding with ethanol solution for 3 weeks and feeding with extra capsaicin and ethanol solution for another 2 weeks, model group show distinct physical sign of gastric hot syndrome. The pathology of gastrics reveals gastricism of model group, while treatment group (treat with Zuojin Wan) shows mild lesion. Elisa detection of model group show that the solution of interleukin-2 (IL-2) is higher than the blank group. The obvious difference among model group, treatment group and blank group reveals the success of the establishment of gastric hot model.
Animals ; Disease Models, Animal ; Drugs, Chinese Herbal ; administration & dosage ; Eating ; Female ; Humans ; Male ; Rats ; Rats, Wistar ; Stomach Diseases ; drug therapy ; pathology ; physiopathology

This study is to establish the gastric cold model of rats. After gastric feeding with cold water for 5 weeks and extra iced water bath in the last 2 weeks, model group show distinct physical sign of gastric cold syndrome. The pathology of gastrics reveals gastricism of model group, while treatment group(treated with Fanzuojin Wan) show mild lesion. Elisa detection of model group show that the solution of interleukin-2 (IL-2) is higher than blank group. The difference with significance among model group, treatment group and blank group reveals the success of the establishment of gastric cold syndrome.
Animals ; Cold Temperature ; Disease Models, Animal ; Female ; Humans ; Male ; Rats ; Rats, Wistar ; Stomach ; chemistry ; metabolism ; pathology ; physiopathology ; Stomach Diseases ; metabolism ; pathology ; physiopathology

Objective To evaluate the surgical effects of laparoscopic pancreaticojejunostomy in pigs and investigate the learning curve. Methods A total of 40 porcine cases of laparoscopic pancreaticojejunostomy were performed by a single surgical team from September 2012 to February 2013.According to the operation sequence, pigs were divided into 4 groups with 10 in each group.For analyzing the efficacy of surgery in different stages, the operation time and efficacy of anastomoses were compared between these groups. Results The operation was finished successfully in all the 40 cases.There were statistical significances in operation time among the four groups.The group Ⅳ had significant shorter time (112.7 ±8.3) min than those in the group Ⅰ [(145.1 ± 16.6) min, q=9.154, P<0.05], the groupⅡ[(133.0 ±8.7) min, q=5.735, P<0.05], and the group Ⅲ[(137.2 ±9.0) min, q=6.922, P<0.05], respectively.There was no difference between the group Ⅱ and the group Ⅰ in operation time (q=3.419, P>0.05), so as to other groups.The results of anastomoses among the four groups were not significantly different (χ2 =0.586,P=0.900). Conclusions Laparoscopic pancreaticojejunostomy in pigs is feasible.The learning curve of the operation is about 30 cases.

Objective To analyze the risk factors of intracranial infection after neurosurgery and the effect of vancomycin.Methods 28 cases of craniotomy in this study, The First Hospital of Ningbo City from January 2011 to July 2014 during the years after the occurrence of intracranial infection patients as the research object, a retrospective analysis of the age, gender, emergency, basic diseases, mastoid airroom, ventricle drainage, posterior fossa surgery, reoperation and operation time (more than 4h).The clinical data, summarize the risk factors of postoperative intracranial infection in department of neurosurgery and to observe the clinical effect of vancomycin in the treatment of such patients.Result The high risk factors of intracranial infection include: Open mastoid gas room during operation, ireoperation, ventricular drainage and operative time (more than 4h).In 28 cases with intracranial infection after craniotomy, the total effective rate of vancomycin treatment was 100.00%.Conclusion The high risk factors of intracranial infection after operation include open surgery, mastoid room, reoperation, ventricular drainage and longer operation time.Vancomycin is an effective drug in the treatment of intracranial infection in the department of neurosurgery.

Objective To investigate the clinic value of enhanced CT-guided percutaneously transthoracic lung biopsy. Methods A total of 423 patients who underwent CT-guided percutaneously transthoracic lung biopsy were retrospectively analyzed. The percentage of specimen are satisfactory,the rate of sample with pathological findings,the number of cases with complications,the average goes of unsuccessful biopsy and the times of changing puncture point were compared between the enhanced CT group and the plain CT group. Results Compare parameters in patients whose procedure were guided with the enhanced CT group and with plain CT group, the average goes of unsuccessful biopsy, the times of changing puncture point in patients, the percentage of specimen that were satisfactory, the rate of sample with positive pathological findings and the rate that complicated with pneumothorax or hemorrhage show statistical significance.(3.08±1.05 vs 3.75±1.34, 8.52%vs 18.54%,90.13%vs 73.60%, 76.62%vs 63.36%, 15.51%vs 27.53%, 7.76%vs 14.04%, P<0.05). Conclusion In patient whose biopsys were guided by enhanced CT, the percentage of samples that are satisfactory is higher, the incidence of complication is fewer and the sample with pathological finding is higher compared to those in patients whose biopsys were guided by plain CT. Therefore, enhanced CT-guided percutaneous transthoracic biopsy is more valuable than plane CT.

Objective To explore the clinical prognostic value of rapid detection for plasma NT-proBNP levels on admission in patients with acute myocardial infarction.Methods 56 patients with AMI were measured plasma NT-proBNP imediately in hospital,and then they were divided into A,B and C group according to NT-proBNP levels (A group:＜ 500ng/L,B group:500-2 000ng/L,C group:＞ 2 000ng/L).The incidence of major adverse cardiac events (MACE including congestive heart failure,malignant arrhythmia,cardiogenic shock and sudden cardiac death) in subjects were observed respectively during hospitalisation and 30 days.Results The three group subjects with different NT-proBNP levels presented different incidence of MACE(A group:1,0;B group:3,1;C group:8,6) at duration of hospital stay,30days (x2 =6.705,P =0.035 ; x2 =7.957,P =0.008).With the NT-proBNP levels rising in AMI paitents,the inciedence of MACE increased.The incidence of MACE in A,B and C group were 6.6％,18.18％ and 73.69％ respectively.Conclusion In AMI patients,plasma NT-proBNP levels could predict early MACE incidence,which has an important value to evaluate the early clinical prognosis.

BACKGROUND:Single hematology analysis can only reflect the body injury at a certain time point after radiation damage, but cannot reflect the longer-term cumulative status after radiation damage. OBJECTIVE:To identify the biomarkers in blood and urine in mice after radiation damage with metabolomics method based on nuclear magnetic resonance hydrogen spectrum. METHODS:Forty-eight mice were randomly divided into four groups and received 0 (sham radiation), 3, 9 and 27 Gy radiation. The blood samples were col ected at 24 hours and 5 days after radiation. Another 36 mice were col ected and divided into three groups and received 0 (sham radiation), 9 and 27 Gy radiation, then the urine samples were col ected at 2 days before radiation and 5 days after radiation for 24 hours. The blood and urine samples were analyzed with proton nuclear magnetic resonance spectroscopy.
RESULTS AND CONCLUSION:The content of aspartic aminotransferase and alanine aminotransferase in blood plasma maintained a stable level after 3-or 9-Gy X-ray radiation, but the level of alkaline phosphatase in blood plasma was increased significantly after 9-Gy radiation, which indicating that low-dose head radiation may cause increased radiation damage and repair. The level of total superoxide dismutase in blood plasma was significantly decreased at 5 days after radiation which indicating that head radiation in mice could cause systemic oxidative stress. Meanwhile, the N-hexamolglycine and-thymidine level in the urine samples was significantly increased after different doses X-ray radiation, which can be used as the radiation damage markers in urine samples after head radiation. The 3-hydroxy-2-methylbenzoic acid 3-O-sulfate level in urine samples was increased by 2.5 times after received 9-Gy radiation, which can be used as the specific markers of middle-and low-dose radiation damage;the level of taurine in the urine samples was increased by 20%after 27-Gy radiation, which can be used as the specific markers of high-dose radiation damage.

This paper introduced the position setup and initial recruitment initiated in publicfunded institutions in Beijing, i.e. Beijing Stomatological Hospital. It stated that position setup should fit actual needs and recruitment standards should comply with long-term development strategy. In addition, the paper highlighted such principles as transparency and all-staff involvement, along with the supervising function of departments and experts of the hospital. Thus, position setup and initial recruitment can be smoothly accomplished, forming a long-standing incentive for medical staff.

Child ; Child, Preschool ; Complement C3 ; Disease Progression ; Female ; Humans ; Intercellular Signaling Peptides and Proteins ; blood ; Lipid Metabolism ; Male ; Obesity ; blood