3.Research Progress on Insect-resistant Genes From Plants
Hong-Mei CHEN ; Kun-Zhi LI ; Li-Mei CHEN ;
China Biotechnology 2006;0(11):-
Plant insect-resistantgenetic engineering hasopened a new way to prevent and cure against the insect pest incrop and wood productions. With the progresses instudies, many insect-resistantgeneswere cloned and characterized. The classification of most insect-resistantgenes from plant, the functionalmechanism and the applications of theplantinsect-resistantgenes, the problems and prospectsforusing the plant insect-resistant genes in genetic engineering was described.
5.Feasibility of different xenogeneic (porcine) acellular dermal matrices in the repair of deep burn wounds:a 12-month follow-up
Ming ZENG ; Dinan WEI ; Yan ZHI ; Hong WANG ; Zonghua CHEN
Chinese Journal of Tissue Engineering Research 2016;20(11):1551-1557
BACKGROUND:Skin grafting is crucial for patients with deep burns, but limited source of autologous skin grafts is an existing difficulty.
OBJECTIVE:To investigate the effect of xenogeneic (porcine) acelular dermal matrix in the treatment of deep burn wounds and the feasibility of its application.
METHODS:Forty-one patients with deep burn were divided into two groups according to the intention of the patients, 21 cases in control group and 20 cases in observational group, folowed by autologous split-thickness skin grafting alone or combined with different (porcine) acelular dermal matrix, respectively. After 12 months of folow-up, the graft success rate at postoperative 1, 2, 3, 4 weeks and skin graft contraction rate and wound repair at postoperative 3, 6, 9, 12 months were observed and compared between two groups. Moreover, levels of inflammatory factors were detected and compared between two groups at postoperative 1, 2, 3 months.
RESULTS AND CONCLUSION: The skin graft success rates showed no difference between two groups at postoperative 1, 2, 3, 4 weeks (P > 0.05). The skin graft contraction rates also showed no difference between two groups at postoperative 3, 6, 9, 12 months (P > 0.05). After 12 months of folow-up, no serious scar hyperplasia, but soft texture appeared in the control group. In the observational group, three cases presented with local pigmentation at the early stage, but it gradualy subsided with time; no obvious scar, but only smal, point-like scar, was visible, and the repaired wound exhibited soft touch. No adverse events and death occurred in both two groups. Experimental results show that the treatment of deep burns with autologous split-thickness skin grafting combined with xenogeneic (porcine) acelular dermal matrix is safe and effective, which can improve the quality of wound healing.
6.Application of combination transfer pump to continuous blood purification
Hongjun YANG ; Zhi CHEN ; Lidan GAO ; Hong ZHANG ; Lu KANG
Chinese Medical Equipment Journal 1989;0(04):-
Objective To evaluate the effect of combination transfer pump and single blood pump to 32 cases of renal failure patient who need blood purification therapy.Methods The combination transfer pump and single blood pump were used.According to the clinical requirement,the parameters of transfer pump were set such as therapy time,replacement liquid flow rate,dialysate flow rate,temperature of warmer and filtrate flow rate,etc.Results 32 patients with varying degrees of illness have been alleviated.Patients' acute left heart failure,water and electrolyte turbulences,acid intoxication and azotemia caused by liquid retention were corrected,which ensured a role for further treatment.Conclusion It is the functions of adjustable time and transfusion volume that are used in the combination transfer pump,the task of which is accomplished by several transfer pumps together.This method is easy to implement and can meet the clinical requirement largely without special CBP instrument.
7.Studies on a selective cyclooxygenase-2 inhibitor celecoxib inhibiting growth of human hepatocellular cancer SMMC-7721 cells lines and inducing apoptosis
Guohao XIA ; Hong CHEN ; Zhi WANG ; Liangxi PAN ; Jifeng FENG
China Oncology 2006;0(09):-
Background and purpose:The incidence of hepatoma is high. The outcome of treatment on hepatoma is poor.So we investigated the effect and mechanism of a selective cyclooxygenase-2 inhibitor celecoxib on the proliferation and apoptosis of SMMC-7721 hepatoma cell line. Methods:MTT assay was used to study the inhibitive effect of celecoxib on the growth of SMMC-7721 hepatoma cell. The effect of celecoxib on cell cycle and apoptosis on cells was studied by flow cytometry(FCM).Transmission electron microscopy (TEM) was used to display the morphological change of the SMMC-7721 hepatoma cell . The biochemical character of apoptosis was viewed on the agarose gel electrophoresis.The expression of bax gene and bcl-2 gene were measured by immunohistochemistry.Results:The SMMC-7721 cells were cultured in media that contained 25,50,75,100 ?mol/L celecoxib,by means of MTT, the inhibition rate was(15?3)%,(34.6?2.4)%,56.8?1.0)%,(86.2?0.4)% respectively after 24 hours; but the inhibition rate was (33.4?0.7)%,(66.7?1.8)%,(76.1?2.4)%,(97.3?0.8)% respectively after 48 hours(P
8.Efficacy of blood purification on acute trichloropropane poisoning.
Hong-Jun YANG ; Zhi CHEN ; Li-Dan GAO
Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(1):56-57
Adult
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Female
;
Hemofiltration
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Humans
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Hydrocarbons, Chlorinated
;
poisoning
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Male
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Middle Aged
;
Poisoning
;
therapy
;
Treatment Outcome
10.Clinical observation of chemotherapy combined with yiqi xiaozheng granule in treating advanced malignant tumor.
Hong SUN ; Ping-ping LI ; Yan-zhi CHEN
Chinese Journal of Integrated Traditional and Western Medicine 2003;23(5):382-383
Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Cisplatin
;
administration & dosage
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Drug Therapy, Combination
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Drugs, Chinese Herbal
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therapeutic use
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Female
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Fluorouracil
;
administration & dosage
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Humans
;
Intestinal Neoplasms
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drug therapy
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Lung Neoplasms
;
drug therapy
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Male
;
Phytotherapy
;
Tetrahydrofolates
;
administration & dosage