Objective To develop a method for nou-invasive risk model that can be used in health management system and primary healthcare centers. Methods Based on systematic phenotype measurement and modified superviser-based machine learning, an applicable and low-cost method for non-invasive disease risk prediction model was developed. Invasive risk assessment model for Chinese isehemic cardiovascular diseases (ICVD) was used as a supervised standard reference to provide evidence based non-invasive model with skin resistance on 24 acupeints. Results A total of 4606 cases from a physical examination system were collected to develop the model. For those reasonable evaluation indeies, the coincidence of non-invasive with invasive risk prediction model was > 90% on average level. Conclusions This method could be used to develop non-invasive risk assessment model for the common diseases.

Taking Liaoning Medical University as an example , the paper introduces the Problem -based learning ( PBL) teaching mode into the teaching of the medical information retrieval course for graduate students .It introduces the overall design and arrangement of PBL teaching and the implementation process of teaching .By questionnaire surveys , analysis of individual retrieval reports and assess-ment of PBL teaching effects , it points out existing problems and proposes several measures for optimizing PBL teaching .

Primary hepatic carcinoma (PHC) is one of the most common cancers worldwide and is the leading cause of death in China. Multidisciplinary treatment is widely accepted as the way to improve the prognosis of PHC, and non-surgical therapy now plays a more and more important role. The purpose of this article is to review the current status and progress of non-surgical treatments of PHC, such as TACE, local ablation, radiotherapy and systemic therapy.

Objective To study the effect of chitosan on the growth of the in vitro cultured retinal nerve cells. Methods The retinal nerve cells of SD baby rats were cultured in vitro. The effects of chitosan at 0.065%,1.25%,2.5%,5%,10%,15% on the growth of the retinal nerve cells were measured by MTT method. The blank control group was created. Results 1.25%,2.5%,5%,and 10% chitosan could obviously promote the growth of retinal nerve cells,and 10% chitosan was the optimal concentration for the growth of nerve cells. Conclusion Chitosan can effectively promote the growth of retinal nerve cells,and it is necessary to undertake the further study of Chitosan.

Technology and technique of coronary artery stent have been developed to a certain degree, restenosis rate of metal stent reduces to 15%, but coronary artery stent is still difficult for long-term effect of interventional therapy on coronary atherosclerotic heart disease. Drug-eluting stent is newly discovered during recent years, while it is prospect for preventing restenosis postoperatively. Although clinical efficacy of drug-eluting stent is encouraged, indication is still simple. In addition, some events related to drug-eluting stent remain poorly unknown. This study was designed to compare security between drug-eluting stent and metal bared stent, analyze major factors correlated with security of drug-eluting stent, and investigate the development of drug-eluting stent.

Objective To investigate health behavior level and self-efficacy in patients underwent coronary artery stent implantation,and to discuss the relationship between health behavior and self-efficacy and to offer proof to the intervention strategy of health behavior.Methods HPLP Ⅱ,self-efficacy questionnaire were used as main tools.106 patients underwent coronary artery stent implantation were investigated.Statistics analyses were performed using the Statistical Package for Social Science(SPSS)13.0.Results (1)The score of total HPLPⅡwas 130.08±21.25,the range of that was 84～180.The health behavior level of47.2%patients was general or worse,and that of 52.8%patients was good or better.(2)The score of self-efficacy was 71.37±16.32,the range of thatwas 38～110.(3)There were significant positive relationship in the scores of total HPLP Ⅱ and the six elements,and self-efficacy.The correlation coefficient was 0.389～0.916(P＜0.01).Conclusion The health behavior level of patients underwent coronary artery stent implantation was not in an ideal state.Self-efficacy has a positive effect on health behavior.The importance motivating factor should be attached to nursing practice and education by medical staff,and medical staff should establish and supervise to carry out corresponding intervention measure.

OBJECTIVE To investigate the inhibitory effect of lead acetate on transient receptor potential A1(TRPA1)channel. METHODS TRPA1-mediated calcium influx in mice dorsal root ganglion(DRG) neurons and HEK293 cells expressing nouse TRP1 (mTRPA1) and human TRPA1 (hTRPA1) was recorded by intracellular calcium imaging. TRPA1-mediated currents were detected by two-electrode voltage clamp. RESULTS Lead acetate 3.0 and 10.0μmol·L-1 inhibited external calcium influx in DRG neurons by(36.7 ± 4.1)% and(79.4 ± 3.1)%(n=5),respectively. The inhibitory effect of lead acetate on hTRPA1-mediated current was concentration-dependent. Lead acetate 0.3, 1.0, 3.0, 10.0 and 30.0μmol · L-1 inhibited the amplitudes of currents by(1.0 ± 0.7)%,(11.6 ± 0.8)%,(57.7 ± 3.2)%,(93.6 ± 2.6)%and(91.2±2.0)%(n≥4),respectively,with the IC50 2.4μmol·L-1. CONCLUSION TRPA1 channel may be an endogenous target of lead. Lead acetate inhibits TRPA1 channel at a very low concentration.

Beginning with the principle of justice, this article analyzed the reasons of medical disputes due to the lack of the principle of justice from four aspects including patients, social media, medical institutions, and medical staff. Corresponding countermeasures were put forward based on the causes of the disputes: fostering cor-rect consciousness of medical treatment, persisting on objective propaganda and creating a favorable environment, strengthening the construction of medical ethics and increasing health investment, strengthening the professional competency education for medical staff, and setting up medical dispute mediation organizations dominated by the third party.

Objective To investigate the expressions of amyloid precursor protein and β-microtubule protein,and to study their clinical significance in patients with cerebral infarction.Methods 108 cases of acute cere-bral infarction as the observation group and 60 normal adults as the control group were collected in the study.The expressions of amyloid precursor protein and β-microtubule protein in different clinical characters were detected by western blot.Results The results of observation group [APP:(168.78 ±13.64)μg/L,β-tubulin:(124.64 ± 27.08)μg/L]were significantly higher than those in control group (t =22.81,P <0.01;t =21.08,P <0.01).Their expressions were correlated with CT scores and prognosis.Conclusion The higher expressions of amyloid precursor protein and β-microtubule protein may promote the occurrence and development in patients with cerebral infarction. The joint detection should be helpful to predict the prognosis and direct the treatment.

Objective To explore the efficacy and side effect of CAG (G-CSF, aclarubicin and cytarabine) priming chemotherapy for patients with acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS). Methods 54 patients with AML at diagnosis and relapse or MDS were'enrolled for the initial treatment with CAG regimen. Patients who have achieved complete remission (CR) were treated with various regimens. Results The total effective rate was 72.2 %, complete remission rate was 48.1% and partial remission rate was 24.1%. The incidence of granulocyte deficiency was 40.7 %(22/54). The severe infection rate was 24.1%(13/54). One case died of function damage in liver. The study includes 36 patients below 60 years, 18 patients above 60 years, and overall effective cases are 28 (77.8 %), 11 (61.1%),respectively. There was significant difference (P ＜0.05). Conclusion CAG regimen is effective and well tolerated in remission for AML and MDS-RAEB.