AIM: The method of the double qualitative similarities and the double quantitative similarities was carried out for the evaluation of the chromatographic fingerprints and applied to HPLC fingerprints of Rhizoma anemarrhenae. METHODS: The chromatographic fingerprints were obtained by injecting 5 ?L of the sample solution each time on a CenturySIL C_(18) BDS column(20 cm?4.6 mm,5 ?m) with the gradient elution solvent system composed of 1% acetic acid-water and 1% acetic acid-acetonitrile.The column temperature was maintained at(30?0.15) ℃ and the detection wavelength was set at 265 nm.The chromatographic fingerprints were evaluated by the normalized similarity S_F and normalizing similarity S′_F and the projecting similarity C% and quautitative similarity P%, changes in characteristics were investigated in the cases of big peaks-free and small peaks-free respectively. RESULTS: 21 co-possessing peaks were selected as the fingerprint peaks of Rhizoma anernarrhenae by choosing rutin peak as the reference peak.S_F could clearly reflect the chemical constituent distribution,and C% could reflect the total contents of the sample,but both of them were seriously influenced by the big peaks and could not reflect the missing of small peaks.S′_F and P% were equivalent weight to all fingerprint peaks,and they could reflect sensitively the small peaks drop-out. CONCLUSION: The double qualitative similarities and the double quantitative similarities are composed of S_F,S′_F,C% and P%,so the changes or lacks of both big peaks and small peaks can be punctually simultaneously monitored to be a novel method for evaluating fingerprints.The HPLC fingerprints can be useful in the quality control of Rhizoma anemarrhenae.

ObjectiveTo observe effects of modulated medium frequency low frequency current hydroelectric bath therapy on sequela of ankle fracture.Methods32 sequela patients of ankle fracture were treated with ZM-C type intellectual faculties intermediate frequency therapeutics instrument. Modulated medium frequency square all wave,the frequency of carry wave was 4 KHz,and the frequency of modulated wave was 100 Hz. The water temperature was 40℃. 30 minutes was a time,12 times was a course. Before and after treatment,results were contrasted themselves.ResultsAfter treatment,32 patients all got satisfactory effects,the shorter of the course,the better of the effect.ConclusionThe modulated medium frequency low frequency current hydroelectric bath therapy is a simple,safe and better effect method for sequela of ankle fracture.

ObjectiveTo investigate the short-term effect of health education intervention on the recovery of new schizophrenia patients. Methods82 patients were randomly divided into observation group, in which patients accepted routine antipsychotic medication, general nursing and system health education intervention, and control group, in which patients accepted antipsychotic medication and general nursing. Brief psychiatric Rating Scale (BPRS) and Positive And Negative Syndrome Scale (PANSS) were used to assess the effects. ResultsThere was no difference in the score of every factor before intervention (P>0.05), but it became different after intervention (P<0.01 or P<0.05). ConclusionHealth education intervention can improve the effect on schizophrenia.

Adhesives ; Anti-Bacterial Agents ; Dental Cements ; Dental Materials ; Humans

Objective:To observe the effect of the proteininhibitor of activated STAT 3 (PIAS3) on the proliferation and apopto-sis of U251 glioma cells after PIAS3 expression was inhibited by RNAi. Methods: Three RNAi expression vectorstargeting PIAS3 were constructed and transfected into CHG-5 cells by liposomein vitro. The most efficient RNAi vector was subsequently selected by examiningthe mRNA expressions of PIAS3 in the transfected cells by semi-quantitativeRT-PCR. The selected RNAi vector was then transfected into U251 cells. After 48h of transfection, the mRNA and protein expressions of PIAS3 in glioma cellswere examined by semi-quantitative RT-PCR and western blot. Apoptosis wasobserved by flow cytometry using a double-staining method with FITC-con-jugatedannexin V and PI. Flow cytometry was also applied in cell cycle assay. Results:RNAidownregulated the mRNA (P<0.01) and protein (P<0.01) expressionsof PIAS3 in transfected cells.RNAi promoted the resistance of U251 cells to apoptosisand subsequently al-tered the cell cycle. A high percentage of G2 phaseand a low percentage of Sphase were observed in U251 cells. Conclusion:The down-regulation of PIAS3arrested U251 cells in the G2 phase andinduced the resistance of U251 cells to apoptosis.

Arteriovenous Malformations ; therapy ; Child ; Female ; Humans ; Pulmonary Artery ; abnormalities ; Pulmonary Veins ; abnormalities ; Septal Occluder Device

OBJECTIVE: To establish an HPLC method for the content determination of prostaglandin A1 and prostaglandin B1 in Alprostadil for injection.METHODS: The determination was performed on Alltech Alltima C18 column with mobile phase consisted of phosphate puffer(pH=6.3)-acetonitrile-methanol(70 ∶ 25 ∶ 5) at a flow rate of 1.5 mL? min-1.The detection wavelength was set at 196 nm.The column temperature was set at room temperature and the injection volume was 20 ?L.RESULTS: The prostaglandin A1 and prostaglandin B1 were well separated from main component and other impurities.The linear range of prostaglandin A1 and prostaglandin B1 were 0.175～19.00 ?g?mL-1(r=0.999 7) and 0.23～19.90 ?g?mL-1(r=0.999 2).The contents of prostaglandin A1 in 3 batches of samples were 4.7%,4.9% and 4.3%,and the contents of prostaglandin B1 in 3 batches of samples were 0.6%,0.8% and 0.5% respectively.CONCLUSIONS: This method is proved to be simple,specific and suitable for the content determination of related substances in Alprostadil for injection.

Acupuncture Therapy ; Adult ; Aged ; Female ; Gastroesophageal Reflux ; therapy ; Humans ; Male ; Middle Aged ; Treatment Outcome

Objective To investigate epidemiological and clinical features of patients with clonorchiasis sinensis infection. Methods The epidemiological, clinical, laboratory and treatment data of clonorchiasis patients in Department of Infectious Diseases, Second Affiliated Hospital, Harbin Medical University from 2002 to 2004 were retrospectively analyzed. Results Sixty-eight point two percent patients had a history of eating raw freshwater fish and shrimp. Patients with occupations as cook, fish stock man, fishing man and fishmonger accounted for 22.6%. The transmission route was not clear in 9.1% of patients. The common clinical manifestations were abdominal pain (60.3%), fatigue (52.3%), diarrhea (33.0%), anorexia (69.3%), jaundice (23.9%), hepatomegaly (59.1%), splenomegaly (8.0%), dizziness (20.9%), fever (5.7%) and biliary colic (14.8%). Twenty-eight point four percent were asymptomatic. Other findings included liver dysfunction (70.4 %) and eosinophile granulocyteosis(69.3 %). Negative rate of stool egg of clonorchis sinensis after treatment with praziquantel or albendazole was 91.9% or 86.5%, respectively. There was no significant difference between the two groups ( χ2 = 0.561, P = 0.454). Conclusions Clinical manifestations of patients with clonorchiasis sinensis are complicated and often accompanied with liver dysfunction. Clonorchiasis sinensis is often misdiagnosed and should be paid much attention.

OBJECTIVE: To investigate the clinical effect of acupuncture combined with repetitive transcranial magnetic stimulation in the treatment of children with spastic cerebral palsy with spleen-kidney deficiency, as well as its effect in improving cerebral hemodynamics. METHODS: A total of 220 children with spastic cerebral palsy were divided into observation group and control group using a random number table, with 110 children in each group. The children in the control group were given rehabilitation training and repetitive transcranial magnetic stimulation, and those in the observation group were given acupuncture in addition to the treatment in the control group. Acupuncture was performed at Zusanli (ST36), Xuanzhong (GB39), Sanyinjiao (SP6), Pishu (BL20), Shenshu (BL23), Qihai (CV6), Quchi (LI11), Neiguan (PC6), Hegu (LI4) and Tianshu (ST25) once every other day, three times a week for 3 consecutive months. The two groups were compared in terms of Gross Motor Function Measure (GMFM), Fine Motor Function Measure (FMFM), comprehensive function score for children with cerebral palsy, clinical outcome, and related cerebral hemodynamic parameters (mean blood flow velocity [Vm], systolic peak velocity [Vs], and resistance index [RI] of the cerebral artery). RESULTS: After treatment, both groups had significant increases in the scores of GMFM, FMFM and comprehensive function (cognitive function, speech function, motor ability, self-care, and social adaptability,P<0.01), and the observation group had significantly better improvements in the scores of GMFM (domains A, B and C), FMFM (domains B, C, D and E), and comprehensive function than those of the control group (P<0.01). The therapeutic effect of the observation group (93/110, 84.55%)was superior to that of the control group (80/110, 72.73%, P<0.05). The observation group had significantly higher Vs and Vm and a significantly lower RI than the control group (P<0.01). CONCLUSION: In the treatment of children with spasmodic cerebral palsy with spleen-kidney deficiency, acupuncture combined with repeated transcranial magnetic stimulation can significantly improve their motor function, comprehensive function, and clinical outcome, which may be associated with the regulation of cerebral hemodynamics.