1.Effects of Mizoribine on Renal Tubular Epithelial-mesenchymal Transition in Unilateral Ureteral Obstruction Mice
Fang YU ; Haiyue DENG ; Hong JIANG
Journal of China Medical University 2016;45(8):728-732,738
Objective To observe the effects of mizoribine(MZR)on renal tubular epithelial?mesenchymal transition(EMT)of mice which have been performed unilateral ureteral obstruction(UUO),and study the mechanism of its anti?fibrosis of renal interstitial. Methods A total of 24 CD1 mice were randomly divided into sham group,UUO model group and MZR treatment group,with 8 mice in each group. The day before op?eration,mice of MZR treatment group had been given MZR 10 mg/kg/d lavage,those of sham group and UUO model group had been given equal saline lavage. Fourteen days after the operation ,blood was collected and serum creatinine and blood urea nitrogen were measured;the obstruction kidneys were harvested for section,HE staining and Masson staining were employed to observe the changes of kidney pathological;the expression ofα?SMA and E?Cad in kidney with detected by immunohistochemical and Western blot method. Results Compared with sham group,serum creatinine and blood urea nitrogen of mice in UUO model group and MZR treatment group were significantly elevated ,kidney pathological chang?es and the expression ofα?SMA in renal tissue were increased and that of E?Cad was reduced ,the differences were all statistically significant(P<0.05);compared with UUO model group,mice in MZR treatment group had different degree of improvements in serum creatinine,blood urea ni?trogen and kidney pathological changes ,the expression ofα?SMA in renal tissue was inhibited and that of E?Cad was increases ,and the differences were statistically significant(P<0.05). Conclusion MZR may inhibit the development of renal tubular EMT in UUO mice ,thereby reduce the level of renal tubule interstitial fibrosis and improve renal function.
2.Isolation, culture and identification of endothelial cells from rat glomeruli.
Yu ZENG ; Hong DENG ; Xiao-jun ZHOU ; Yun WANG
Chinese Journal of Pathology 2005;34(4):233-234
Animals
;
Antigens, CD34
;
metabolism
;
Cell Culture Techniques
;
methods
;
Cells, Cultured
;
Endothelial Cells
;
cytology
;
metabolism
;
Female
;
Kidney Glomerulus
;
cytology
;
metabolism
;
Male
;
Rats
;
Rats, Sprague-Dawley
;
Vimentin
;
metabolism
3.Therapeutic effects analysis of patients with stage Ⅲ gastric carcinoma administrated fluorouracil implants during radical gastrectomy
Hongzhi LUO ; Zonghai HUANG ; Yuaulong YU ; Hong CHEN ; Huizhou DENG
Cancer Research and Clinic 2009;21(5):338-340
Objective To investigate the therapeutic effects of fluorouracil implants in Stage Ⅲgastric carcinoma patients after radical gastreetomy. Methods The general state of health and cumulative survival of 200 stage Ⅲ gastric carcinoma patients undergone radical gastrectomy from January, 1999 to, December 2002 in the People's Hospital of Zhongshan City were analyzed, in which only 120 patients administrated fluorouracil implants during radical gastreetomy. The therapeutic effects of fluorouracil implants in stage Ⅲ gastric carcinoma patients after radical gastrectomy were observed. Results The differences of weight (P = 0.041), HGB(P =0.024), Plt(P =0.017), CEA(P =0.001), CA19-9(P =0.003) were significant between two groups. The incidence rate of nausea/emesis in group T (28 %) was higher than that in group O(17 %, P = 0.006), and incidence rates of myelosuppression(P =0.81), diarrhea(P =0.72), hepatic function lesion(P =0.97) and renal function lesion(P =0.20) were no significant. The cumulative 5-year survival rate of group T(45 %) was higher than that in group O (15 %, P =0.002). Conclusion Fluorouracil implants can improve the therapeutic effects on gastric carcinoma patients undergone radical gastrectomy.
4.Effect of glomerular intercellular interaction under high glucose concentration on production of ROS and TGF-?_1 in co-cultured ECV304 cells and intervention with tea polyphenols
Hong DENG ; Hailang LI ; Kai WANG ; Yu ZENG ; Bicheng LIU
Chinese Journal of Pathophysiology 1986;0(02):-
AIM: To investigate the effect of glomerular intercellular interaction under high glucose concentration on the production of reactive oxygen species (ROS) and transforming growth factor ?_1 (TGF-?_1) in co-cultured human ECV304 cells, and to study the intervention with tea polyphenols (TPs). METHODS: The endothelial cells were cultured alone or co-cultured with mesangial cells in high glucose media with or without TPs for 0 h, 12 h and 36 h, respectively. The activity of SOD and the content of MDA in the media of the system were detected by spectrophotometry. The expression of TGF-?_1 mRNA in the endothelial cells was measured by using semi-quantitative reverse transcription PCR (RT-PCR). RESULTS: High glucose decreased the activity of SOD, increased the content of MDA and up-regulated the expression of TGF-?_1 mRNA in co-cultured ECV304 cells and the effect became more prominent than the single-cultured cells. TPs interrupted it more effectively. CONCLUSION: These data suggest that there is interaction between mesangial cells and ECV304 cells under high glucose concentration. The interaction may markedly up-regulate the production of ROS and the expression of TGF-?_1 in co-cultured ECV304 cells. TPs may protect ECV304 cells by intervening intercellular interaction.
5.Expression and significance of Bcl-2, Bax, Fas and caspase-3 in different phases of human hemangioma.
Hong, YANG ; Chenguo, DENG ; Shengguo, SHEN ; Duanlian, ZHANG ; Ying, YU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2006;26(4):402-4
The relationship between Bcl-2, Bax, Fas, caspase-3 and development of hemangioma and the molecular mechanism was investigated. By using immunohistochemical S-P method, proliferating cell nuclear antigen was detected. According to the classification of Mulliken in combination with PCNA expression, 27 cases were identified as proliferating hemangioma and 22 cases as involutive hemangioma. Five normal skin tissues around the tumor tissue served as controls. By using immunohistochemical technique, the expression of Bcl-2, Bax, Fax and Caspase-3 was detected. The cells expressing Bcl-2, Bax, Fax and cappase-3 were identified as hemangioma endothelia by immunohistochemical staining of VIII factor. The average absorbance (A) and average positive area rate of Bcl-2, Bax, Fas and caspase-3 expression were measured by using HPIAS-2000 imaging analysis system. The results showed that the expression of Bcl-2 in the endothelia of proliferating hemangioma was significantly higher that in involutive degenerative hemangioma endothelia and vascular endothelia of normal skin tissue (P < 0.01). The expression of Bax, Fas and Caspase-3 in the endothelia of involutive hemangioma was obviously higher than in the endothelia of proliferating hemangioma and normal skin tissue (P < 0.01). The expression of BAx and Fas in endothelia of proliferating hemangioma was higher than in those of normal skin tissue (P < 0.05). It was suggested that Bcl-2, Bax, Fas and caspase-3 might be involved in the development and involution of hemangioma. Bcl-2 could promote the growth of hemangioma by inhibiting apoptosis of endothelia. Bax, Fas and caspase-3 promote the switch of hemangioma from proliferation to involution by inducing the apoptosis of hemangioma endothelia.
6.Antisense phosphorothioate oligonucleotide targeting bFGF enhances chemosensitivity of human laryngeal squamous carcinoma cells
Hong-Liang HUANG ; Hong WANG ; Jun-Jian XIANG ; Yong TANG ; Ning DENG ; Hong-Yu YANG ;
Chinese Journal of Cancer Biotherapy 2006;0(05):-
Objective:To study the enhancing effect of bFGF-targeted antisense phosphorothioate oligonucleotide (APO)on the chemosensitivity of human laryngeal squamous carcinoma cell line Hep2 to Doxorubicin,5-Fluorouracil, and Cisplatin.Methods:bFGF-specific APO was designed,constructed and transfected into Hep2 cells with jetPEI (polyethyleneimine).Expression of bFGF mRNA was evaluated by semi-quantitative RT-PCR after transfection;immuno- cytochemical method was used to examine the expression of bEGF expression before and after transfection of Hep2;the in- duction of cell apoptosis was analyzed by flow cytometry;cell proliferation was then analYzed by MTT assay after treatment with bFGF-specific APO or chemotherapeutic drugs,or a combination of both.Results:bFGF-specific APO inhibited the growth of Hep2 cells in a dose-and time-dependent manner,with the peak inhibitory rate being 25.5%.The expression of bFGF mRNA and protein decreased by 52.0% and 41.1%,respectively.The apoptosis rate of Hep2 cells was 20.5% after transfection,bFGF-specifie APO reduced the 50% inhibitory concentration of Doxorubicin,5-Fluorouracil,and Cis- platin in Hep2 cells by 75.5%,83.5% and 65.4%,respectively.Conclusion:bFGF-specific APO can enhance the chemosensitivity of Hep2 cells,which paves a new way for potential biologic chemotherapy of laryngeal squamous carcino- ma.
7.Baicalin inhibits PDK1 to mediate glucose metabolism reprogramming and intervene rheumatoid arthritis synovial inflammation
Yu-long ZHU ; Yan-hong BU ; Ran DENG ; Yan WANG ; Pei-rong GAN ; Hong WU
Acta Pharmaceutica Sinica 2023;58(5):1165-1172
This study started from the effect of baicalin (BC), the main active component of the labiaceae plant
8.Mesenchymal chondrosarcoma of thoracic spine: report of a case.
Xiang-lei HE ; Deng PAN ; Yu ZHOU ; Yong-li GAN ; Hong-bin ZHANG
Chinese Journal of Pathology 2006;35(12):766-767
12E7 Antigen
;
Antigens, CD
;
metabolism
;
Cell Adhesion Molecules
;
metabolism
;
Child, Preschool
;
Chondrosarcoma, Mesenchymal
;
metabolism
;
pathology
;
surgery
;
Diagnosis, Differential
;
Humans
;
Immunohistochemistry
;
Male
;
Neuroblastoma
;
metabolism
;
pathology
;
Sarcoma, Ewing
;
metabolism
;
pathology
;
Spinal Cord Neoplasms
;
metabolism
;
pathology
;
surgery
;
Spinal Neoplasms
;
metabolism
;
pathology
;
Thoracic Vertebrae
;
Vimentin
;
metabolism
10.Clinical observation of growth hormone intrauterine perfusion combined with replacement cycle in the treatment of thin endometrium
Hong YU ; Huizhen TANG ; Zhaohua LIU ; Huilian CHEN ; Zhaohui DENG ; Tingting TANG ; Xiaoli WU
Journal of Chinese Physician 2017;19(3):371-375
Objective To investigate the effects of the frozen embryo transplantation for patients with poor outcome of endometrial growth by using growth hormone (GH) intrauterine perfusion combined with replacement cycle in the treatment of thin endometrium.Methods This was a prospective study and study participants were consecutively recruited between Jun 2014 and September 2015.A total of 88 frozen thawed embryo transfer cycles was divided into two groups from the Reproductive Center of Hunan Provincial Maternal and Child Health Hospital.Group A were 63 hormone replacement therapy (HRT) cycles and Group B were 25 GH intrauterine perfusion combined HRT cycles.Results The endometrial thickness of 22 thin endometrium patients from Group B were increased above 7 mm on progesterone day.The endometrial thickness on transplant day of Group A was (9.28 ± 1.64) mm,which was significantly higher than Group B (7.9 ± 0.86) mm (P < 0.05).The clinical pregnancy rate (50.79% vs 52.0%),implantation rate 31.1% vs 47.17%),miscarriage rate (9.38% vs 15.38%) had no significant difference between Groups A and B.The endometrial thickness from 7 mm to 7.9 mm on transplant day,the clinical pregnancy rate (30.76% vs 54.54%) had no significant difference in two groups (P >0.05),but the implantation rate of group A was significantly lower than that of group B (20% vs 52.17%) (P <0.05).When the endometrial thickness was above 8 mm on transplant day,the clinical pregnancy rate (58.33% vs 63.63%),implantation rate (36.36% vs 50%) had no significant difference between groups A and B (P > 0.05).Conclusions GH uterine cavity perfusion was a useful method for treatment of thin endometrium,and was helpful for improvement of endometrial thickness and receptivity,improved embryo implantation environment by assistance for HRT under the high estrogen levels.