Humans ; Mycoplasma pneumoniae ; Pneumonia, Mycoplasma ; microbiology

Objective To investigate the recent prognosis of stress hyperglycemia for the emergency percuta- neous coronary intervention(PCI)with ST-segment elevation acute myocardial infarction(AMI).Methods 410 pa- tients treated by emergency PCI were chosen.According to a history of diabetes and blood glucose levels,they were randomly divided into four groups:group 1(n = 288):random blood glucose normal and non-diabetic patients;group 2(n = 30):random normal blood glucose in diabetic patients;group 3(n = 54):random plasma glucose level and non- diabetic patients;group 4(n = 38):high random plasma glucose level in diabetic patients.Age,gender,hospital within 24 hours with random glucose,glycated hemoglobin(HbA1c),peak creatine kinase,TIMI flow recovery and 30-day mortality of the patients were compared.Results Mortality of four groups were 4.2%,3.4%,7.5% and 5.9% re- spectively(P

Objective:To optimize the extraction technology of volatile oil in myrrh. Methods: Using the extraction quantity of volatile oil as the index, the volatile oil was extracted by an essential oil extractor, and U6 (64 ) uniform design was used to investigate the influencing factors such as the immersion time ( A) , the mesh number ( B) , the extraction time ( C) and the additive water amount ( D) . Results:The optimized extraction technology was as follows: myrrh was crushed to obtain 10 mesh powder, soaked for 10 mi-nutes with 8-fold amount of water, and then extracted 3 hours. Conclusion:The quantity of volatile oil extracted from myrrh is accu-rate, the technology is reasonable, and the research provides scientific basis for the extraction of volatile oil in myrrh.

Objective To investigate effect of tacrolimus on renal function, blood lipids, cytokines and peripheral HMGB-1 and NF-κB in nephrotic syndrome patients.Methods From January 2012 to January 2014 in the Fifth Affiliated Hospital of Zhengzhou University, 127 cases of patients with nephrotic syndrome were included based on random number table, and divided into two groups (n=65) and control group (n=62).Control group with conventional symptomatic treatment, observation group in combination with tacrolimus treatment on the basis of control group, which were treated for a total of two courses, eight weeks for a course of treatment.Serum creatinine and blood urea nitrogen levels, TG, TC, HDL-C, LDL-C levels, serum IL-1, TNF-αlevels and serum HMGB-1 and NF-κB levels were evaluated.Results The serum creatinine and blood urea nitrogen levels after treatment was significantly lower than that of control group, respectively ( P<0.05 ) .TG, TC and LDL-C levels after treatment in observation group was significantly lower than that of control group respectively (P<0.05).Serum IL-1 and TNF-αlevels after treatment in observation group was significantly lower than that of control group respectively (P<0.05).Serum HMGB-1 and NF-κB levels after treatment in observation group was significantly lower than that of control group respectively (P<0.05).Conclusion Tacrolimus can reduce blood lipid levels, inflammatory reaction and serum HMGB-1, NF-κB levels in nephrotic syndrome patients, and significantly improve renal function.

Objective To investigate effect of alprostadil on serum levels of inflammatory cytokines and nutritional biochemical indexes in patients with chronic renal failure.Methods According to the random number table, 129 patients with chronic renal failure patients were included in this group and randomly divided into observation groups (n=67) and control group (n=62).Control group were given conventional symptomatic treatment, and the observation group were given alprostadil treatment on the basis of control group with a course of four weeks of both groups.The Scr, BUN, IL-6, TNF-α, CRP, Ca2 +, ALB, P3 +, Hb, RBC, hemorheology changes were compared before and after treatment.Results The Scr, BUN levels after treatment was significantly lower than before treatment (P<0.05), and Scr, BUN levels in observation group after treatment was significantly lower than control group (P<0.05).Serum levels of IL-6, TNF-α, CRP after treatment significantly decreased than before treatment (P<0.05), serum IL-6, TNF-α, CRP after treatment was significantly lower than control group ( P<0.05 ).After treatment, Ca2 +, ALB levels of observation group was significantly higher than control group and P3 +was significantly lower than control group (P<0.05), while there were no significant differences of Hb, RBC.The hemorheology of observation group before treatment was significantly lower than control group ( P<0.05 ) .Conclusion Alprostadil can significantly improve renal function, and the possible mechanism may be associated with reducing serum levels of inflammatory factors and improving blood rheology, which delay progression of chronic renal failure effectively.

Objective To find the cells in myocardium controlling in cardiomyogenic differentiation of marrow derived-mesenchymal stem cells(MSCs).Methods Rat marrow derived-MSCs,cardiomyocytes(CM)and endothelial cells(EC)were isolated and cultured,then MSCs were incubated with the CM or EC or cultured alone after labeled by 5-Bromo-2-deoxyuridine(BrdU)for 72 hours.The cells were identified by morphology,immunocytochemistry and double immunocytochemistry.Results More than 95% of the cells isolated and cultured belonged to MSCs,CM or EC.Incubated with CM,most MSCs labeled by BrdU showed cardiomyocyte-like morphology.After 4 weeks of cultivation,positive staining of double immunocytochemistry of BrdU and Sarcomeric Actin or Connexin-43(CX-43)were observed in 44% cells.However,there was no obvious change in morphology and no positive staining of double immunocytochemistry was recorded in MSCs incubated with EC or cultured alone after 1、2、3 and 4 weeks of cultivation.Conclusion MSCs can differentiate into cardiomyogenic cells in cardiomyocyte microenvironment and cardiomyocytes may play a crucial role in the differentiation.

Objective To explore the effect of JiTT teaching-mode based on the Moodle on the undergraduate teaching of surgical nursing,and to reform the traditional undergraduate nurse teaching mode.Methods Totally 84 nursing undergraduates of Grade 2008 were randomized into the control group and the experimental group.The control group was given traditional teaching-oriented method,while the experimental group was applied JiTT teaching-mode based on the Moodle.Results In the experimental group,83.6％ recognized the JiTT teaching-mode,and 76.4％ felt satisfactory with the new teaching method.They thought the integrated check-up evaluation method and records distribution was more appropiiate.The nursing examination suggested that the average grade of the experimental group was significantly higher than the control group.In the experimental group,71.5％ thought the examination were less hard,though more study time was spending.6.7％ refused the new method because the higher requirement on the online study.78.4％ thought the in and out classroom study was more appropriate,it helped to cultivate the ability of the team work cooperation and the communication.Conclusions The JiTT teaching-mode based on the Moodle platform applying in the surgical nursing can construct an effective,communicative online course,stimulate the learning interest,cultivate the ability of exploration and cooperation,and also promote the learning revolution and high quality nurses cultivation.

Objective To construct eukaryotic expressing vector of the mouse NMNAT1 (nicotinamide mononucleotide adenylyl-transferase) gene and examine its ability to express the NMNAT1 gene in Hela cells.Methods The full-length NMNAT1 cDNA sequence was amplified by PCR and cloned into the plasmid of T-vector and then to pcDNA3.1 construct.The recombinant plasmid pcDNA3.1-NMNAT1 was identified by DNA sequencing and then transfected with Lipofectamine2000 into Hela cells.The expression of NMNAT1 was detected by real time quantitative PCR (qPCR) and Western blot after 48 h transfection.Results The recombinant eukaryotic vector carrying NMNAT1 gene was constructed successfully in a match with database and this vector could up-regulate the expression of the NMNAT1 gene both in mRNA and protein levels in Hela cells.Conclusions The eukaryotic vector carrying NMNAT1 gene (pcDNA3.1-NMNAT1) enhances the expression of NMNAT1 gene.

Objective To retrospectivly analyze the value of Pap smear,liquid-based cells, colposcopy and colposcopic cervical biopsy in the diagnosis of cervical cancer. Methods 116 cases of cervical cancer were retrospectively analyzed, and the value of Pap smear,liquid-based cells ,colposcopy,and colposcopic cervical biopsy in the diagnosis of cervical cancer were investigated. Result 116 patients were pathologically diagnosed with cervical cancer before and after surgery. Pap smear was performed for 30 patients (25.9%) ,and 10 patients (33.3%) were detected with HSIL. Liquid-based cells was performed for47 patients (40.5%) ,and 25 patients (53.1%) were detected with HSIL. Colposcopy was performed for 99 patients (85.3%), and 75 patients (75.8 % ) were detected with HSIL or invasive cancer. Preoperative colposcopic biopsy was performed for 99 patients, and the pathological coincidence rate was 94.9%. There was no significant difference statistically between two cytology screening ( P ＞ 0.05 ). There was significant difference statistically between liquid-based cells and colposcopy ( P ＜ 0.01 ). There were significant differences statistically among liquid-based cells,colposcopy ,and colposcopic cervical biopsy ( P ＜0.01 ). Conclusion Cytology, colposcopy, colposcopic cervical biopsy could not be individually more complete and accurate detection of cervical cancer. Colposcopy,colposcopic biopsy than cytology had higher accurate rate of diagnosis of cervical cancer. A reasonable combination of multiple detection methods could improve the diagnostic accuracy of cervical cancer.

Aim To observe the protection effect of Tribulus terrestris saponin monomer B (TTSMB) on cadiocytes impaired by hypoxia-reoxygenation (H/R).Methods Cadiocytes of neonate rat were cultivated for 72 hours and divided into normal control group, H/R group,GSTT 100 mg·L~(-1) group and TTSMB 10,1,0.1 nmol·L~(-1) group.Morphocytology change of cadiocytes was observed after the treatment.Cadiocyte survival rate was detected with MTT colorimetric method.Levels of creatine kinase (CK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), malondialdehyde (MDA), and activity of superoxide dismutase (SOD) were determined. Apoptosis rate was detected with flow cytometry.Expression of caspase-3 was examined with western blot.Results Compared with the control group, the survival cell number in H/R group decreased obviously, content of CK,LDH, AST, MAD increased, and activity of SOD decreased (P<0.01).Compared with the model group, the survival cell population increased in TTSMB 10,1,0.1 nmol·L~(-1) group (P<0.05 and P<0.01).Contents of CK, LDH, AST, MAD decreased, whereas the activity of SOD increased (P<0.05 and P<0.01).Apoptosis rate and expression of caspase-3 also reduced in TTSMB 10,1,0.1 nmol·L~(-1) group.Conclusion TTSMB has significant cadiocytes protective effect against H/R and inhibits cadiocyte apoptosis,and the mechanism depends on the effect against oxygen free radical.