2.Survey of the relationship between pathogen strains distribution and nusocomial infection
Xia GAO ; Hong ZHANG ; Xiaoping XU ; Chao QIN ; Chongyou WANG
Chinese Journal of Primary Medicine and Pharmacy 2009;16(1):51-53
Objective To ostimate the distribution of microorganism strains isolated from hospital ward cir-curmstanee and clinical specimens, and then proceed to inquire into their interrelation to nosocomial infection, provi-ding evidence for preventing and reducing nosocomial infection. Methods Specimens were collected from hospital circumstance and each clinical laboratory, and then made a bacterial identification and pathogen strains survey. Re-sults There was a closed correlation between the microorganism strain isolated from hospital circumstance and clini-cal infection pathogen(86%). Conclusion A series of disinfection management measures should be made and for-mulated so as to reduce the hospital infection rate as far as possible.
5.Juxtaglomerular cell tumor with malignant features: report of a case.
Min YAO ; Yang XIA ; Shi-fan CHEN ; Hong-wen GAO
Chinese Journal of Pathology 2012;41(7):485-486
Actins
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metabolism
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Adult
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Antigens, CD34
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metabolism
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Diagnosis, Differential
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Female
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Follow-Up Studies
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Humans
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Hypertension
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etiology
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Juxtaglomerular Apparatus
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pathology
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surgery
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ultrastructure
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Kidney Neoplasms
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complications
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metabolism
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pathology
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surgery
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ultrastructure
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Nephrectomy
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Vimentin
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metabolism
6.Practice and Experience on the Comprehensive Experiment Teaching of Physiology
Xingji YOU ; Xin NI ; Wenyuan GAO ; Jinhui XIA ; Hong HAN
Chinese Journal of Medical Education Research 2006;0(08):-
Comprehensive and designed experiment is beneficial to improving and developing students' comprehensive quality and innovation ability.In the last two years,we have carried out the comprehensive and designed experiment of physiology among the seven-year program students,which combine book knowledge and clinics.It has proved that this teaching mode can not only help students master the knowledge systematically but also lay a good foundation for them to analyze the clinical problems.
7.Migration and Survival of Rat Neural Stem Cells Marked by Green Fluorescent Protein after Transplanted to Injured Spinal Cord
Chuanchuan GAO ; Lei XIA ; Shuyu HAO ; Junhua LI ; Hong WAN
Chinese Journal of Rehabilitation Theory and Practice 2008;14(4):341-342
Objective To investigate the migration and survival of neural stem cells(NSCs)in vivo.Methods NSCs cultured in vitro were transfected by lentiviral vectors expressing green fluorescent protein(GFP)to construct GFP-NSCs,then trans-seeded into lactide-co-glycolide(PLGA)scaffold and implanted into the injured site of T9 spinal cord in rat.One month after transplantation,the migration of NSCs in spinal cord was examined by fluorescence microscope,and the survival rate of NSCs was counted out.Results NSCs labeled GFP had strong expression of green fluorescence.One month after transplanting,part of NSCs expressing GFP could be seen in PLGA scaffolds and rostral,caudal spinal core.The survival rate counted out was 1.4911±0.0313%.Conclusion NSCs marked by GFP and transplanted to rat injured spinal cord could migrate into the spinal cord tissues and the minority of them could survive.
8.Epidemiological study of chronic kidney disease with children of gender difference
Mindong GAO ; Xia GAO ; Hong XU ; Yipa SAI ; Xiaofeng CAO ; Wenping YOU ; Xiaoyan LEI
International Journal of Pediatrics 2015;42(2):207-209
Objective To investigate the positive ratio of urine and gender difference of chronic kidney disease(CKD) with children in Lanzhou,a heavy pollution and underdeveloped city in northwest of China.Methods Through the Multistix 10 SG,the morning urine of the children were detected,which aged from 12 to 13 years old.Results The positive ratio of urine was 14.9% in subjects,18.2% in girls and 12.8 % in boys respectively.Furthermore,the positive ratio in girls showed statistically significant higher than boy's.(x2 =21.77,P < 0.01).Espcially,the ratios of hematuria and pyuria significantly increased in girls (x2 =17.52,P < 0.01;x2=7.95,P < 0.01).Conclusion The gender difference of the positive rate of urine was existed in our large samples.And there is a higher prositive rate of hematuria and pyuria in the girls.This investigation will enrich the epidemiological data of CKD in children(12 ~ 13 years old)in Lanzhou city.
9.Effects of ischemic pre- and postconditioning on cerebral glycogen synthase kinase-3 beta activity in a rat model of cerebral ischemia-reperfusion
Bo ZHAO ; Zhongyuan XIA ; Wenwei GAO ; Jiabao HOU ; Yang WU ; Hong GAO ; Changjian WU
Chinese Journal of Anesthesiology 2010;30(11):1391-1393
Objective To investigate the effects of ischemic pre- and postconditioning on cerebral glycogen synthase kinase-3 beta (GSK-3β) activity in a rat model of global cerebral ischemia-reperfusion (I/R).Methods Forty male Wistar rats weighing 200-230 g were randomly allocated into 4 groups (n =10 each) : Ⅰ group sham operation (group S); Ⅱ group I/R; Ⅲ group ischemic preconditioning (group IPR) and Ⅳ group ischemic postconditioning (group IPO). The animals were anesthetized with intraperitoneal 10% chloral hydrate 0.4 ml/100 g. Global cerebral ischemia was induced by four-vessel-occlusion in group Ⅱ , Ⅲ and Ⅳ. Bilateral vertebral arteries were cauterized and bilateral carotid arteries were occluded for 10 min. In group IPR cerebral ischemia was preceded by 3 cycles of 10 s ischemia followed by 30 s reperfusion. The group IPO received 3 cycles of 30 s reperfusion followed by 10 s ischemia at the end of 10 min cerebral ischemia. The animals were killed 2 days later. The brains were immediately removed for determination of neuronal apoptosis in the cortex (by TUNEL), the infarct size (by TTC), p-GSK-3β activity (by spectrum assay) and the expression of Bcl-2, Bax and Caspase-3 (by SP). Linear correlation of p-GSK-3β activity with the number of apoptotic neurons in the cortex and cerebral infarct size was analyzed. Results Cerebral I/R significantly increased the number of apoptotic neurons in the cortex and infarct size, decreased p-GSK-3β activity, down-regulated Bcl-2 expression and up-regulated Bax and Caspase-3 expression in group I/R as compared with group S. Ischemic pre- and postconditioning significantly attenuated these cerebral I/R-induced changes. The p-GSK-3β activity was negatively correlated with the number of apoptotic neurons in the cortex and cerebral infarct size. Conclusion Ischemic pre- and postconditioning reduces cerebral I/R injury through inhibiting the activity of GSK-3β.
10.~1H MR spectroscopy of C6 glioma after tumor necrosis factor-?therapy
Hong YIN ; Yuangui GAO ; Xiang ZHANG ; Dakuan GAO ; Xia LI ; Haitao ZHAO ;
Chinese Journal of Radiology 2001;0(03):-
Objective To observe the therapeutic effect of tumor necrosis factor ?(TNF ?) on C6 glioma using 1?H MR spectroscopy, and to evaluate the practical value of 1?H MR spectroscopy in observing therapeutic effect Methods C6 cell mixed with low boiling point argarous were inoculated in caudate nuclear in 33 rats by using sterotatic method By using high magnetic field spectrometer, high resolution 1?H MR spectroscopy was carried at a 3 day interval until 15 days after treatment with TNF ? The relative choline containing compound (Cho),N acetyl aspartate (NAA),creatine (Cr), Cho/Cr,NAA /Cr,and NAA/Cho were analyzed with statistic method Results The Cho decreased obviously 3 days after TNF ? treatment( P