Scientific research fund is an important component of scientific research management,and an important precondition for scientific research activity. This paper explores the existing problems in the scientific research fund management of medical university, and suggests that only standardization of the fund management can guarantee the efficiency the fund use, and realize the scientificity, normalization, rationality of the research fund management, which will all contribute the improvement of research quality.

Objectives:To observe the effects of enteral nutrition on cell immune in patients with acute renal failure. Methods:The changes of cell immune were compared in 22 patients with acute renal failure before and after EN treatment. Results:There were significant improvements in cell immune after 10 days of EN. CD3,CD4,CD4/CD8 and NK acitivity( P

At present, too much importance has been attached to avian influenza globally. The reasons for this and the possibility of contagion from human to human were discussed . As to prevention and control of avian influenza, suggestions were made to find early human cases in local hospitals and local CDC (Center for Disease Control and Prevention), to make the laboratory staff follow the bio-safety principle for diagnosis of avian influenza.

Objective To develop a convenient and effective molecular authentication method for identifying Dendrobium nobile from other species of Dendrobium Sw.Methods Based on rDNA ITS sequences of D.nobile and other species of Dendrobium Sw.of Huangcaos and Fengdous,unusual sequence sites were confirmed and an allele-specific diagnostic primer pair was designed for D.nobile. Diagnostic PCR was performed using the primers with the total DNA of D.nobile and other species of Dendrobium Sw.as templates to establish the positive PCR condition for D.nobile.Results An about 300 bp DNA positive fragment was amplified from D.nobile with anneal temperature at 66 ℃ and anneal time at 40 s,whereas no any DNA fragment was amplified from the rest 39 species of Dendrobium Sw.Conclusion D.nobile could be effectively distinguished from other species of Dendrobium Sw.by diagnostic PCR with allele-specific diagnostic PCR,which is not only effective and specific,but also simple and timesaving,and has a comprehensive application prospects.

OBJECTIVE:To provide the reference for the set-up of precautions against flu pandemic in China.METHO_DS:RNA of influenza viruses isolated from flu children in flu seasons between 2003 and 2005 in Beijing was extracted and amplification of M2 gene fragments associated with ion channel were performed by one step reverse transcription–polymerase chain reaction(RT-PCR).The sequencing of amino acid was determined,and its bioinformation was analyzed with software,then the positions of amino acid for resistance to amantadine were detected.RESULTS:20 of 31 isolated strains were resistant to amantadine,the percentage of which was 66.7%.CONCLUSION:The monitoring of drug resistance to flu viruses should be strengthened in China.

Objective:To explore the relations between freshmen's self-identity status,personality characteristics and mental health status.Methods:188 new students were asked to complete the questionnaires of mental health scale and personality scale from the China college students mental health evaluation system,and eollnge students self- identity status scale.Results:①the most status of identity is achievement(43.1%),then the identity diffusion (30.9%);②male students in compare with female,were more likely in achievement status(60.7?13.8/53.9?11.4,P0.406);③the achieve- ment status had obvious negative correlation with depression,dependency(r=-0.17～-0.24),the diffusion status had obvious positive correlation with depression,social-withdrawal,and impulsion(r=0.15～0.34);④active,ac- commodating,tough-minded personality characteristics had obviously negative correlation with mental symptoms(r= -0.16～-0.56).Conclusions:encourage college students to form the achievement ego-identity status,to be ac- tive,accommodating and tough-minded,will be helpful for their psychological health.

AIM: To evaluate and analyze the morphological property and physiological activity of cartilaginous loose bodies in the knee joint, to research the changeable mechanism of histological morphous and physiological function. METHODS: The experiment was conducted at the Department of Pathology of the First Affiliated Hospital of Anhui Medical University, the Physical Testing & Chemical Analysis Center of Anhui Medical University, and the Electron Microscope Center of Anhui Provincial Hospital Affiliated to Anhui Medical University from January 2005 to January 2006. ①Experimental samples were 30 cartilaginous loose bodies all through arthroscopy as experimental group in which Group A (less than 6 months) had 14 samples and group B (between 6 and 12 months) 10, group C (more than 12 months) 6. Another 30 control samples were originated from non-weight loading side of articular cartilage through special equipment by arthroscopy. ②All samples were carried out the morphological measurement, light and transmission electron microscope exam, Ⅱ-collagen immunohistochemical stain, the measurement of collagen, calcium and fosforus. RESULTS: ①To compare the samples in the group A and control group, they had the identical or similar morphosis, microscopic structure of chondrocyte and distribution of extracellular matrix, the statistical treatments of the content of calcium, fosforus and collagen showed that the differences of scores between two groups were not statistically significant [calcium:(3.120?0.224),(3.090?0.231) mg/g;fosforus:(0.432?0.045),(0.455?0.067) mg/g;collagen:(462.43?17.12)(466.71?14.59) mg/g]. General speaking, comparing with the control articular cartilage, the loose bodies in group A had the normal histological property and physiological activity. ②Compared with control group, the samples in group B and C had the tendency of fibrous degeneration: necrotic or degenerative chondrocytes increasing, the contents of proteoglycan and Ⅱ-collagen decreasing, the tendency in group C more than group B, but the cartilaginous loose bodies in group B and C had many active chondrocytes mainly. Total content of collagen [(441.20?16.49),(417.33?8.38) mg/g] and Ⅱ-collagen decreased, while the contents of calcium and fosforus increased [calcium: (3.696?0.442),( 6.469?0.555) mg/g;fosforus:(0.523?0.068),(0.683?0.056) mg/g]. Generally, compared with the control articular cartilage, the histological property and physiological activity of the loose bodies in groups B and C had changed. CONCLUSION: The cartilaginous loose bodies can remain its histological property and physiological activity in the knee articular cavity for a long time, but it has the tendency of disorganization and physiological activity descending as time prolongs.

There are some common problems such as recurrent symptoms and poor compliance among patients with chronic diseases. Although scientific knowledge and skills have been learned from traditional health education, patients with chronic diseases cannot improve their life style continuously. At present, many researches abroad have proved that health coaching contributes to setting feasible goals and encourages continuously behavior change of patients. In China, health coaching is in the stage of becoming familiar with its scientific definition, intervention effect and application characteristics. And health coaching also needs evidences from high-level evidence-based studies. Along with continuous improvement of intervention techniques on health management, health coaching will get further development and standardization.

Dry eye is a most common external eye disease in clinic and it can cause ocular discomfort and visual disturbance. The conventional examinations for dry eye have some shortcomings, for example, high variability and insufficient association with symptoms. Recently, routine ophthalmic technologies play more extending roles in evaluation of dry eye, including corneal topography, wavefront aberrometer, optical coherence tomography (OCT) and confocal microscope. Corneal topography and wavefront aberrometer could be used to evaluate the stability of tear film and visual function. OCT could image tear meniscus directly, and confocal microscope could observe histopathology of ocular surface in vivo. The studies on ophthalmic technologies in dry eye make us deepen the understanding of pathophysiology of dry eye and also provide new diagnostic approaches.

AIM:To observe the effects of advanced glycation end-products (AGEs) on thioltransferase (TTase) expression and activity in human lens epithelial cells.
METHODS: Human lens epithelial cells B3 ( HLE B3 ) were treated with 1. 5mg/mL AGEs - BSA as the experimental groups cultured by fetal bovine serum of volume fraction 10% dulbecco modified eagle medium ( DMEM) and bovine serum albumin ( BSA) was added at the same concentrations as the negative control. The level of reactive oxygen species ( ROS ) was evaluated. Cells were collected at 0, 1, 2, 3, 4d and total RNA or protein was extracted. TTase mRNA levels were detected by qRT-RCR. TTase expression was detected by Western blot and its activity was measured.
RESULTS: Compared with the control group, AGEs-BSA up-regulated the expression of ROS (P<0. 01), ROS content increased in a time-dependent manner. BSA had no effects on ROS expression. The expression of TTase increased after treatment with AGEs-BSA for 1d, peaked at 2d (nearly 5. 06-fold increase, P<0. 01), then decreased gradually. No change was observed between BSA and control group (P>0. 05). Similarly, TTase activity peaked at 3d (nearly 2. 01-fold increase, P<0. 01). Western blot test found that TTase protein expression was increased
gradually, starting from the 3d TTase expression was reflected that there was statistically significant difference compared with control group (P<0. 05).
CONCLUSION:AGEs-BSA significantly increases the production of ROS in human lens epithelial cells, and it then induces the oxidative stress which may promote the expression of TTase and enhances the activity of TTase.