No abstract available.
Aged* ; Diabetes Mellitus* ; Humans

Frequent anomalies causing a left-sided appendix are situs inversus and malrotation. The ectopic position of the appendix frequently causes a delay or error in diagnosis because of atypical symptoms. A physical examination is of limited value unless dextrocardia is present. A left-sided appendix is a rare anomaly, but when other diseases are ruled out, it must be considered especially at the emergency department.
Appendicitis* ; Appendix ; Dextrocardia ; Diagnosis ; Emergency Service, Hospital ; Physical Examination ; Situs Inversus

BACKGROUND: The proliferation of vascular smooth muscle cell(VSMC) is a part of the major pathogenic mechanism for atheroscle- rosis. It has been reported that L-type calcium channel plays a role in the VSMC proliferation in diabetic rats. But there is a little study results about the association between L-type calcium channel and VSMC proliferation by glucose concentrations in culture media. So we examined the association between voltage-dependent L-type calcium channel of VSMCs and the proliferative activity of vascular smooth muscle cells. METHODS: Rat aortic VSMCs were isolated from the aorta of OLETF rat by enzyme method. VSMCs were cultured in various concentrations of glucose(5.5, 25 mM). The VSMCs(1x104 cells in 24-well plates) were incubated in the presence of Bay K 8644 (10-6M) with/without verapamil(10-6M) for 48 hours. Then the proliferation was assessed by MTT(methylthiazole tetrazolium) assay and expression of L-type calcium channel mRNA was measured by RT-PCR. RESULTS: The proliferative ability and the expression of L-type calcium channel of cultured VSMCs were increased dose-dependently by the glucose concentrations(p<0.05). Bay K 8644 enhanced the proliferation of VSMC and verapamil blocked the incremental effects induced by Bay K 8644. CONCLUSION: These results suggest that L-type calcium channel may play a role in VSMC proliferation of OLETF rat.
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester ; Animals ; Aorta ; Calcium Channels, L-Type* ; Cell Proliferation* ; Culture Media ; Glucose* ; Muscle, Smooth, Vascular* ; Rats ; Rats, Inbred OLETF* ; RNA, Messenger* ; Verapamil

No abstract available.
Acute Kidney Injury*

BACKGROUND: Some research viewed that effective dietary therapy was enough to control proper blood glucose level, but in the most patient, dietary therapy was not practiced and the most difficult part of managing their diabetes. The purpose of this research study was to investigate dietary practice adherence and perceived barriers among the elderly diabetes. METHODS: The survey was mailed to 852 persons with diabetes member via diabetic educator of 156 hospitals or clinics and 24 health centers. Questionnaire had background information of patients, meal regularity, food intake as a dietary practice adherence, barriers of 36 items which have 3 areas such as motive/attitude, knowledge, authority/resource. We asked the person with diabetes to rate barrier to dietary practice adherence. 432 questionnaire were returned the response. we selected 69 persons who were over 65 year old. RESULTS: 1) meal regularity was more satisfactory than food intake. 2) deficit of meal regularity were evening snack and resonable spacing between evening meal and evening snack. 3) deficit of food intake was serving of milk. 4) main barrier to dietary practice adherence was that of knowledge. CONCLUSION: For practice effectively dietary therapy to elderly diabetes, individual consultation or small group education must be pursued and more easily educational skills should be required.
Aged* ; Blood Glucose ; Eating ; Education ; Humans ; Meals ; Milk ; Postal Service ; Surveys and Questionnaires ; Snacks

Authors presented the clinical review and evaluated the therapeutic response to 20 eyes of filamentary keratitis observed at the Department of Ophthalmology, St. Mary's Hospital for last 3 years. 1. The filaments disappeared within 2 days in 11 eyes by mechanical debriment with 0.5% C-M eyedrops, tear substitutes and prednisolone eye drops. 2. 4 of 20 eyes showed disappearance of the filaments within 3 days by the subconjunctival tathion injection combined with instillation of tathion eye drops. 3. For one eye which was resistant to the above therapy treated with continuous wearing of the B and L soft contact lens. In this case the filaments reduced in number from one day and completely disappeared after 6 days' wearing of soft contact lens. Patient discontinued the wearing of soft contact lens one week after. No epithelial filaments have appeared after discontinuance in wearing of the soft contact lens one week later.
Contact Lenses, Hydrophilic ; Humans ; Keratitis* ; Ophthalmic Solutions ; Ophthalmology ; Prednisolone

No abstract available.
Pemphigoid, Bullous*

Human epidermal cells were obtained from suction blisters of 14 healthy individuals, and were cultured for 24-96 hours st a concentration of 1x 10(7)/ml, 5 x 10(6)/ml, 1 x 10(6)/ml, 5 x 10(5)/ml. Cells were also cultured with or without stimulants such as phorbol myristic acetate(PMA), muramyl dipeptide(MDP), and endotoxin. Then, cell-free supernatants of cultured epidermal cells were tested for ETAF by a thymocyte prolifera.tiom assay. The results were as follows : 1, The highest activity of ETAF was produced by fresh epidermal cells(EC) at a concentration of 1 x10(7)ml. Its highest 3H-TdR was 4928+/-2480cpm. The highest activity of ETAF was produced by cultured EC at a concentration of 5 x10(6)/ml. Its highest 3H-TdR was 13983+/-8045 cpm. 2. The highest activity of ETAF was produced by fresh EC with n culture time of 24 hours. Its highest 3H-TdR was 5357+/-3760cpm. The highest activity of ETAF was produced by cultured EC with a culture time of 72 hours. Its highest 3H-TdR was 11905+/-5327cpm. 3. The highest activity of ETAF was produced by both fresh and cultured EC at a titer of 1: 8 dilution of cell-free supernatants. 1ts highest 3H-TdR was 4928 +/-2480cpm in the fresh EC, and 11905+/-5327cpm in the cultured EC. 4. Alhen fresh EC was stimulated with PMA, MDP and endotoxin, higher activity of ETAF was found in the group stimulated with PMA or MDP compared with its control group. But lower activity of ETAF was found in the group stimulated with endotoxin compared with its control group. The 3H-TdR was 6000+/-1936 cpm in the group stimulated with PMA, 6945+/-3182 cpm in the group stimulated with MDP, and 36943+/-36861cpm in the group stimulated with endotoxin.
Blister ; Humans* ; Suction ; Thymocytes

No abstract available.
Breast Feeding* ; Breast* ; Statistics as Topic*

It is known that the activated peripheral T lymphocytes are increased in patients with autoimmune disorders such as systemic lupus erythematosis, rheumatoid arthritis, multiple sclerosis and autoimmune thyroiditis, but not in the patients with myasthenia gravis on previous studies. We investigated the subsets of T cells in peripheral blood of the patients with myasthenia gravis using flow cytometric analysis. Forty-three patients of myasthenia gravis who were not on steroid or other immunosuppressants were chosen, and thirty-six age-matched healthy persons were evaluated as controls. The peripheral blood mononuclear cells from patients and controls were stained with FITC or PE-conjugated monoclonal antibodies to several surface molecules expressed on T cells within 6 hours after collection. The samples were analysed by flow cytometry within 24 hours. In patients with myasthenia gravis, the expressions of DR and CD25 molecules on the T lymphocytes were increased significantly compared to those of the control group. The expression of CD25 was increased on CD4+ T cells, but not on CD8+ T cells . The expression of DR molecule was increased on CD8+ T cells, but not on CD4+ T cells. Therefore we suggest that the activated T cells are increased in myasthenia gravis and CD25 surface markers on CD4+ T cells may be a more sensitive indicator of immune status.
Antibodies, Monoclonal ; Arthritis, Rheumatoid ; Flow Cytometry ; Fluorescein-5-isothiocyanate ; Humans ; Immunosuppressive Agents ; Multiple Sclerosis ; Myasthenia Gravis* ; Receptors, Interleukin-2 ; T-Lymphocyte Subsets* ; T-Lymphocytes ; Thyroiditis, Autoimmune