Objective: To investigate the effect of two modes of one-lung ventilation(OLV) on lung lobectomy in pediatric patients.Metheds: Twenty ASA Ⅰ-Ⅱ patients(8 male,12 female) aged 2~14 year,weighing 10~30 kg,and undergoing lung lobectomy were enrolled in this study.The patients were premedicated with intramuscular Sod Luminal 3~5 mg/kg and Atropine 0.01 mg/kg im,30 minutes before induction.Induction was performed with Dorcum 0.1 mg/kg,Fentanyl 5 ?g/kg and Vecuronium 0.1 mg/kg,maintained with Vecuronium 70 ?g/(kg?h) and Propofol 5mg/(kg?h) iv boluses.Ordinary tracheal tube was inserted into bronchus(14right,6 left) during OLV.First,OLV with volume-control mode maintained 25 min,and then,OLV with pressure-control mode was performed.In first step Vt was set at 8~10 ml/kg and respiratory rate was adjusted to maintain PetCO2 between 30~40 mmHg.In second step the inspiratory pressure was set according to the plateau pressure during step 1,hemodynamics and PaO2 were measured and recorded.Results : During OLV with pressure-control mode PaO2 was significantly increased,as compared with that during volume-control mode(P

Objective: To study the expression of Echinococcus multilocularis 14-3-3 antigen encoding gene in Bacillus Calmette-Guerin(BCG).Methods: The recombinant plasmid pBCG-Em14-3-3 was introduced into BCG by electroporation and the bacteria with pBCG-Em14-3-3 were cultured to logarithm growth period,then induced daily by heating at 45℃ for 30min in the last three days.The expression products were analyzed by SDS-PAGE and Western blot.Results: The Em14-3-3 recombinant protein was successfully expressed by pBCG-Em14-3-3 in BCG as an obvious band of 27kDa by SDS-PAGE,which accounted for 11% of total bacterial protein in BCG.Conclusion: The pBCG-Em14-3-3 could be expressed efficiently in BCG and be combined with specific antibody in mice sera,which suggested that the Em14-3-3 recombinant protein expressed by rBCG-Em14-3-3 vaccine was of specific antigenicity.

Objective:To summarize the anesthesia experience of open heart surgery in 43 infants(weigh less than 5kg) and to explore the related issues with myocardioprotection and brain protection.Methods:Conventional induction of anesthesia,nasotracheal intubation,arteriovenous puncture,cardiopulmonary bypass were established,according to the needs for maintaining the body low temperature(deep,middle,superficial);haemodynamics was maintained with vasoactive agent after aorta was opened.Results:Two infants with transposition of conducting arteries succumbed to stoma leak.One infant with tetralogy of Fallot succumbed to low cardiac output syndrome,bellows complication appeared in 8 cases,Ⅲ.atrial ventricular block 1,and the rest infants recovered well.Mortality was 6.98%in this group.Conclusion:Cardiac anesthesia control in infants with low weight is difficult,death rate is hiqh and complication is complex.Butas long as control is done well,mortality omd complitation can be devreased.

Objective:To study the clinical value of susceptibility weighted imaging(SWI)in brain diseases.Methods:37 cases with blood vessel pathological change of brain diseases were collected and analyzed,including 8 example of angioma cavernosum,10 cerebral infarction,15 brain tumor,2 venous malformation and 2 venous sinus thrombus.Conventional T1WI,T2WI,DWI,the SWI and enhanced T1WI,MRA images were performed to compare the superiority of the SWI images to the others in demonstrating the small hemorrhagic focuses,the small vein,hemosiderin,calcification and other paramagnetic materials.Results:SWI could distinguish the hemorrhage from the blood vessel and discover the smaller hemorrhagic focuses of the angioma cavernosum.It could discover more tiny veins adjacent to the dilated draining vein.SWI could detect small hemorrhages in acute cerebral infarction and small draining veins in brain tumor.And draining veins in deep part and expanded rete venosum in the surfaces of brains could be obviously demonstrated when sinus venosus thrombus occurred.Conclusion:SWI is a new pulse sequence that can demonstrate the abnormal low current capacity blood vessels,the structure of small vein,the tiny hemorrhages as well as ferrugination and calcium deposition sensitively.So it can be applied in the diagnosis and antidiastole of the central nervous system diseases as an important supplement to the MRI conventional sequences.

AIM: To study the mechanism and protective effect of rosmarinic acid from Rosmarinus officinalis extract on acute hepatic injury induced by carbon tetrachloride in mice. METHODS: Mouse model of acute hepatic injury was set up by giving CCl_4 abdominally.The effects on ALT,AST avtivities,TG level in serum and MDA level in liver tissue were examined.Histological change and pathological change were observed by means of hematoxylin and eosin staining.The body weight,liver weight of each mice were measured. RESULTS: Rosmarinic acid significantly reduced the activities of ALT,AST,level of TG in serum and level of MDA in liver tissue,restrained the enlargement of liver and reduced the necrosis in pathological observation. CONCLUSION: Rosmarinic acid shows significant inhibitory effects on liver injury induced by CCl_4 in mice.The mechanisms may be related to its effects of reducing lipid peroxidation product.

Objective:To investigate the influences of different OD value and centrifugal parameters on the ultrastructure of Escherichia coli in order to find out the best OD value,speed and time of centrifugation.Methods:According to orthogonal experimental designing principle,the E.coli with different OD600 values are divided into 9 different groups of centrifuge speed and time.The changes of E.coli morphology in each group at different OD600 value,centrifuge time and speed were compared under transmission electron microscope.Results:The different OD600 values and centrifugal parameters have little influence on the ultrastructure of E.coli,but when the OD600 value at 0.8,centrifuge time at 15 min,the cross-section ratio was significantly higher than other groups and was more suitable for observation.Conclusion:The best parameter to prepare the TEM samples of E.coli is under the OD600 value at 0.8 and centrifugation time at 8 minutes and speed at 5 000～12 000 r/min.

This paper introduces general guidelines for designing and constructing a magnetic stimulator (MS). These guidelines cover the theoretical concepts, hardware aspects and necessary components. RLC model producing the impulses in MS is focused on and the way to calculate the parameters of the model is presented. Some factors that influence induced magnetic field are discussed. Experiments are carried through with self-developed round coil and 8-shaped, and Tesla meter is used to detect the magnetic fields. The experiments data show that the distribution of the induced magnetic fields by the two coils are tailored to those of theoretical calculation adopted now. A simulation experiment to measure magnetic field in brain has been completed.

This paper introduces primarily the design of hardware and software systems in infrared automatic handwash basin including the working process of the electric circuit. At the same time, in order not to act mistakenly suffering from exterior interference, the anti-interference function is added to the software, which is also the design technique of this software.

Artificial hip replacement is an effective therapy to eliminate hip pain,correct deformity and restore function.With population aging and improving of people life quality,artificial hip replacement has been widely conducted.Over the past 40 years,artificial hip replacement technique has rapidly developed,and minimally invasive technology presents development direction of future artificial hip replacement.Skilled operation experience and instrument application are two key factors to ensure surgery success.The minimally invasive technology arouses more attention from clinical doctors and patients due to minimal invasion,little blood loss and fast recovery.However,not all patients can undergo minimally invasive hip replacement.This paper summarized related studies on minimally invasive hip replacement in domestic,and discussed the indications,surgical approach,complications and prevention.

Objective To compare the sensitivity of body mass index (BMI),waist-hip ratio (WHR)and percentage body fat (PBF)in the evaluation of the obese adolescents population,and find a more sensitive evaluation method.Methods In this study,2 045 adolescents were recruited in the city of Chongqing by using multiple-stage random cluster sampling,and they were identified as overweight or obesity on the base of BMI,WHR and PBF.Results There was significant difference among the detection rate of the three methods (P<0.05),the detection rate of PBF was the highest (12.9%),then BMI (7.2%)and WHR (6.0%).consistency test showed that the concordance of three methods was poor and Kappa (BMI and WHR)=0.199,Kappa (BMI and PBF )=0.441,Kappa (WHR and PBF)=0.187;comprehensive evaluation was chosen as the gold standard and compared with three meth-ods respectively by ROC curve,the analysis showed that area under curve (AVC):PBF(0.954)>BMI(0.918)>WHR (0.694). Conclusion The sensitivity of PBF and BMI was higher.Although the WHR sensitivity was lower,WHR is a good predictor asso-ciated for chronic diseases,which suggests that the development of appropriate adolescent WHR standard is necessary.