Vertigo is a kind of patients' subjective feelings. The severity of vertigo is closely related to many factors. But we are short of a well accepted quantitative evaluation method capable of accurately and comprehensively evaluating vertigo in clinics. Reducing the onset of vertigo, enhancing the re- covery of equilibrium function, and improving the quality of life of vertigo patients should be taken as the focus of evaluating therapeutic effects. As for establishing a Chinese medical effectiveness evaluation system for vertigo, we believe we should distinguish different "diseases". We could roughly identify it as systemic vertigo and non-systemic vertigo. For systemic vertigo, the efficacy of vertigo could be comprehensively evaluated by UCLA vertigo questionnaire or dizziness handicap inventory combined with equilibrium function testing indices. But for non-systemic vertigo, the efficacy of vertigo could be comprehensively evaluated by taking UCLA vertigo questionnaire or dizziness handicap inventory as main efficacy indices. Secondly, we should analyze different reasons for vertigo, choose symptoms and signs in line with vertigo features as well as with Chinese medical theories, and formulate corresponding syndrome effectiveness standards according to different diseases. We should not simply take syndrome diagnosis standards as efficacy evaluation standards.
China ; Dizziness ; diagnosis ; Humans ; Quality of Life ; Surveys and Questionnaires ; Vertigo ; diagnosis

Animals ; Hippocampus ; metabolism ; Long-Term Potentiation ; Male ; Neurogranin ; biosynthesis ; Rats ; Rats, Wistar ; Sleep Deprivation ; metabolism

With the increasing incidence and prevalence of male sexual dysfunction, andrologists are more and more in need of accurate and efficient tools to assess therapeutic efficacy and patients' satisfaction and to help patients achieve satisfactory treatment results. This article summarizes some of the most commonly used questionnaires for the diagnosis and assessment of the treatment of male sexual dysfunction, including International Index of Erectile Function (IIEF), Erection Hardness Score (EHS), Quality of Erection Questionnaire (QEQ), Erectile Dysfunction Inventory of Treatment Satisfaction (EDITS), Treatment Satisfaction Scale (TSS), Self-Esteem and Relationship (SEAR), Premature Ejaculation Profile (PEP), Premature Ejaculation Diagnostic Tool (PEDT), Index of Premature Ejaculation (IPE), Arabic Index of Premature Ejaculation (AIPE), Aging Male Symptoms Scale (AMS), Androgen Deficiency in the Aging Male (ADAM), and Symptomatic Inventory for Screening Late-Onset Hypogonadism in Males (SILOH), and presents an overview on their clinical application.
Erectile Dysfunction ; Humans ; Male ; Surveys and Questionnaires

Objective To prepare monoclonal antibody specifically against carcinoembryonic antigen glypican-3 (GPC3) and its preliminary application. Methods GPC3 was cloned with PCR to pET16b vector and expressed in E. co-liBL21. Spleen cells were obtained from Balb/c mice embedding immunized with purified antigen intraperitoneally, and fused with Sp2/0 cells. Hybridoma cells were screened by indirect ELISA, and identified by Western blot assay using puri-fied protein after the cell fusion. The indirect immunofluorescence method was used to detect the GPC3 expression in HepG2 cell line. Results The prokaryotic expression vector of GPC3 was successfully constructed, and GPC3 was stably expressed in E. coliBL21. A mouse hybridoma cell line secreting monoclonal antibody to GPC3 was obtained. Western blot analysis showed that monoclonal antibody specifically recognized recombinant protein. Monoclonal antibody could be used to detect GPC3 protein expression in HepG2 cell line by indirect immunofluorescence. Conclusion The monoclonal antibody against GPC3 was successfully obtained.

Objective Investigation of the clearance rate of RBC in cerebral spinal fluid(CSF)after lumber subarachnoid space continuous drainage following subarachnoid hemorrhage(SAH)and the relationship with cerebral vasospasm(CVS) were carried out. Methods An experimental SAH was achieved in dogs by twice injecting fresh autologous arterial blood into cisterna magna of each animal.RBC counts in CSF were measured in the drainage group, puncture group and control group respectively.The degrees of CVS were analyzed through cerebral angiography(%reduction of basilar artery diameter,%RBAD). Results Comparing with puncture group and control group, the drainage group showed a significantly higher clearance rate of RBC with lesser occurrance and degree of CVS. Conclusions RBC and its degeneratives in CSF may be involed in the process of CVS following experimental SAH. Continuous lumber subarachnoid space drainage may prevent CVS.

Objective To study the angiographic anatomic characteristics of renal arteries in Chinese.Methods Selective renal angiography was performed immediately after coronary artery angiography in(1?057) Chinese patients,including 702 males and 355 females.The mean age of them were 59.3?8.3 years.Six French JR4 catheters were used via transfemoral approach.Three to five milliliters nonionic contrast media were injected manually into each renal artery and cine pictures were taken at an anterior-posterior view.Results A single-renal-artery was found in most of the patients(87.23% at the left side and 89.97% at the right side).In the others,two or even more independent renal arteries were found at one or both sides.Most of the renal arteries (87.79% at the left side and 91.20% at the right side) originated between the upper third of the first lumbar vertebra and the upper third of the second lumbar vertebra.The right renal artery ostium was located superior to the left one in 45.13% of the cases.In the other 45.41% of them,renal artery ostia of both sides were at a same level.Conclusion Anatomic variations in the number and origin of renal arteries are not nare in Chinese.Understanding the angiographic anatomic characteristics of renal artery would be useful for renal artery interventions.

Objective To explore the clinical and coronary angiography features of female patients suffering from premature coronary heart disease by analyzing retrospectively the clinical data of those women who undertook coronary angiography before 55 years old in a period of 5 years. Methods Female patients under 55 years were enrolled. Data such as risk factors (including hypertension, diabetes, hyperlipidemia, stroke, and metabolic syndrome), clinical findings (including blood pressure, serum lipid test on admission), and coronary angiographic images were collected to find the relationship between risk factors and coronary heart disease. Results The study had enrolled 166 cases with 71 cases (42.8%) of them diagnosed as coronary heart disease. In this group, the most important risk factors were diabetes, metabolic syndrome, history of hypertension, high level of serum TG and decreased HDL. At the same time, we found that the body mass index, LDLC level, TG level and blood glucose in CHD patients were significantly higher than those in non-CHD ones. Single vessel disease was the most popular pattern of these CHD patients. Conclusion History of diabetes, metabolic syndrome and hypertension were the most vital risk factors of CHD in this relative young female population.

Objective To study the mechanism of CKLF1-plasmid transfer on the myocardial repair in rat AMI models.Methods Eighteen male SD rats were randomly divided into 3 groups and in each separate group,the rats were injected intramuscalary with plasmid DNA encoding CKLF1 gene(n=6),emptyplasmid(n=6)and saline(n=6)with in vivo electroporation respectively.Rats were subjected to left coronary artery ligation on the 6th day after gene transfer and were killed on the 21st day.The expressions of BrdU/?-actin,Ki67/?-actin were assessed by immunohistochemistry.Results BrdU-positive cells in CKLF1 group were more than those in the saline group and the empty plasmid group(cells/HP)(33.11?2.10 vs.14.16?1.63 & 18.46?2.77,P0.05).Conclusion Intramuscular injection with in vivo electroporation of CKLF1 may cause an enhanced myocardial proliferation of acute myocardial infarction tissue in experimental rat.

Objective To investigate the guidance of a new classification on the treatment methods selection for cystic dilation of bile duct (CDBD).Methods The clinical data of 213 patients with CDBD who received treatment according to the Todani 2003 classification at the Peking Union medical College Hospital from September 1968 to July 2013 were retrospectively analyzed.The CDBD was reclassified with a new classification proposed by Dong Jiahong et al,and the guidance of the new classification on the treatment methods selection for CDBD was analyzed.Patients were followed up via out-patient examination and telephone interview till August 2013.Results Of the 213 patients,139 were with Todani type Ⅰ CDBD (type C CDBD of the new classification) ; 3 were with Todani type Ⅱ CDBD (type C1 CDBD of the new classification) ; 1 was with Todani type Ⅲ CDBD (type E CDBD of the new classification); 52 were with Todani type Ⅳa CDBD (35 with type D1 and 17 with type D2 CDBD of the new classification) ; 1 was with Todani type Ⅳb CDBD (type C CDBD of the new classification) ; 8 were with Todani typeⅤ-Ⅰ CDBD (type B CDBD of the new classification) ; 9 were with Todani type Ⅴ-Ⅱ CDBD (type A CDBD of the new classification).Eighteen patients did not receive the surgical treatment.Of the 195 patients who received surgical treatment,patients with type C and D CDBD of the new classification took a large proportion.Patients with type C CDBD of the new classification received cystectomy,biliary cyst resection,Rouxen-Y cholangiojejunostomy or internal drainage.Patients with type D CDBD of the new classification received extrahepatic biliary cyst resection,Roux-en-Y cholangiojejunostomy.Patients with severe intrahepatic disease and with type D1 CDBD of the new classification received concomitant left hemihepatectomy or pancreaticoduodenectomy.Patients with type A1 CDBD of the new classification received right hemihepatectomy.Patients with type A2 CDBD of the new classification were cured by conservative treatment after split liver transplantation.Patients with type B1 CDBD of the new classification received left hemihepatectomy and Roux-en-Y cholangiojejunostomy.Patients with type B2 CDBD of the new classification received bile duct stone extraction.There was 1 patient with type E CDBD,and partial resection of the CDBD ± bile duct reconstruction was carried out.Pancreatic fistula,biliary fistula,reflux cholangitis,cholangitis and anastomotic stricture were detected on 74 patients,and they were cured by conservative treatment or lithotomy.A total of 187 patients were followed up with the median time of 85 months (range,1-432 months).One hundred of seventy-five patients recovered well,and 12 patients with canceration of the bile duct died of tumor metastasis at postoperative 1-282 months.Conclusion This new classification simplifies the typing of extrahepatic bile duct dilation,refines the typing of intrahepatic bile duct dilation,and has better guidance for surgical treatment.

The status quo of American informatics nurses investigated 4 times by US HIMSS from 2004 to 2014 was analyzed.The following characteristics of American informatics nurses were summarized in terms of their educa-tion level, income, qualification authentication, occupation satisfaction, work experience and duties, including high education level and interdisciplinary background, high income and occupation satisfaction, necessary clinical background, and systematic professional development, which may provide reference for nursing staff in China to have a better understanding of informatics nurse.