Adolescent ; Adult ; Child ; Chronic Disease ; Female ; Humans ; Male ; Middle Aged ; Mucin-5B ; biosynthesis ; Nasal Mucosa ; metabolism ; Sinusitis ; metabolism ; Young Adult

Objective To explore the relationship between respiratory viruses infection and the episode of steroid responsive simple nephrotic syndrome(SRSNS).Methods Thirty-eight children with SRSNS were selected,including the active stage group(n=28)and the remission group(n=10).The urine from 18 cases of nephritic nephrosis,16 cases of bronchiolitis,15 cases of secondary glomerular diseases and 15 cases of healthy children were served as controls.By using the method of reverse transcriptase-polymerase chain reaction(RT-PCR)and alkaline phosphoesterase-anti alkaline phosphoesterase enzyme-linked assay(APAAP),viral genes and antigens in urine were assayed,respectively.Results The positive rate of detecting viruses in the active stage group of SRSNS children was higher than that in the remission group and the other control groups(Pa

Objective To explore the effects of physical exercises on recurrent seizures-induced long-term learning and memory deficits and on the expression of zinc transporter-3(ZnT-3)in hippocampuses of developmental period rats.Methods Sprague-Dawley rats aged 21 d were randomly divided into blank control group,exercises control group,seizure group and seizure plus exercises group.Abdominal cavity injections of penicillin or saline were used to induce recurrent seizure or as a control in the corresponding groups.During the postnatal(P)39-44 d and P61-65 d periods,Morris water-maze tests were administered to evaluate spatial learning and memory capacity.During the P48-53 d period,the rats in exercises control and seizure plus exercises groups were subjected to a 30-min daily aerobic exercises program for 6 d.The real-time reverse transcription-polymerase chain reaction(RT-PCR)method was used to detect the expression of ZuT-3 mRNA in hippocampuses of all rats at P66 d. Results Searching strategy:There was a decreasing incidence of marginal strategy and an increasing trend in the use of taxis and straight line strategy in all four groups.The scores on d 1 and 4 were significantly higher in two control groups than in two seizure groups in water-maze test(all P＜0.05).By d 2 the exercises control and seizure plus exercises groups were scoring significantly higher than the bland control and seizure groups(P＜0.05).Memory test:The frequency of passing through the platform quadrant decreased significantly in the two seizure groups compared to the two control groups in both probe tests(all P＜0.05).RT-PCR test:ZnT-3 mRNA expressions in hippocampuses were significantly higher in seizure plus exercises group than in any other groups. Conclusions Penicillin-induced recurrent seizures can induce long-term damage on learning and memory capacity in developmerital period rats.Physical exercises can improve learning capacity.It's mechanism may be related to the up-regulation of ZnT-3 expression in hippocampus of rats.

Humans ; Hyoid Bone ; surgery ; Sleep Apnea, Obstructive ; surgery

Objective To evaluate the mechanism of external branch of the superior laryngeal nerve (EBSLN) injury during thyroid surgery as showed by intraoperative neuromonitoring.Methods 70 patients with 109 nerves at risk were enrolled in this study from March 2011 to October 2011.A positive signal is determined by observing contractions of the cricothyroid muscle.The relationship between EBSLN and the upper pole of the thyroid or the inferior constrictor muscle was studied.Results 108 nerves (99.1％) were located successfully,42 of which were visualized (38.9％).55 nerves (50.9％) crossed the superior thyroid artery more than 1 cm apart,while the other 53 nerves (49.1％) went less than 1 cm including 24 nerves(more than 0.5 cm,less than 1 cm) and 29 nerves (less than 0.5 cm) or coursed below the superior pole of the thyroid.The rate of the latter type was significantly elevated in patients with the top to botton diameter of the gland more than 5 cm.One patient suffered from impairing in the production of high tones postoperatively.Conclusions Intraoperative neuromonitoring is useful and helpful in providing instructive information for operations by locating EBSLN.

Intervertebral disc degeneration is considered as a primary cause of clinical low back pain, however the molecular mechanism is not clear yet. Recently, researches on the molecular basis of intervertebral disc degeneration have become a hotspot. The special structure and biomechanics properties of the disc contribute to its propensity toward degeneration. Intervertebral disc degeneration is associated with the changes of the cytological behavior,including the increase in cell death and the degradation of extracellular matrix. However, the mechanism of cell death including cell apoptosis and autophagy in intervertebral disc degeneration remains unclear. Further study on the molecular mechanism of intervertebral disc degeneration is the foundation of improving and treating the intervertebral disc degeneration in the future. Although some progresses are made in the aspect of biological study, the biological environment of intervertebral disc itself is still a challenge for the development of biological treatment. This article is to review the latest advance on the biological characteristics of normal intervertebral disc and the cell death in the process of the intervertebral disc degeneration.
Animals ; Apoptosis ; Cell Death ; Extracellular Matrix ; metabolism ; Humans ; Intervertebral Disc ; cytology ; metabolism ; Intervertebral Disc Degeneration ; metabolism ; physiopathology

Adult ; Antigens, CD20 ; metabolism ; Carcinoma ; metabolism ; pathology ; DNA Nucleotidylexotransferase ; metabolism ; Diagnosis, Differential ; Female ; Follow-Up Studies ; Humans ; Keratins ; metabolism ; Lymphoma ; metabolism ; pathology ; Male ; Middle Aged ; Necrosis ; Seminoma ; metabolism ; pathology ; Thymoma ; metabolism ; pathology ; surgery ; Thymus Neoplasms ; metabolism ; pathology ; surgery ; Tuberculosis ; pathology

OBJECTIVETo explore the therapeutic effect of Chinese medicinal massage with nourishing-Shen and activating-blood manipulation in treating women with climacteric syndrome and its influence on endocrinal function.

METHODSSixty patients were assigned to two groups, the 40 patients in the treated group were treated by Chinese medicinal massage for 20 min once every other day; the 20 patients in the control group were treated by hormone replacement therapy with Premarin 0.625 g, once daily by oral taking. The therapeutic efficacy was evaluated after two-month treatment by changes of serum levels of estradiol (E2), follicular stimulating hormone (FSH), luteinizing hormone (LH), and Kupperman index before and after treatment.

RESULTSKupperman index showed that the total symptom score in the treated group was improved from 30.71 +/- 8.43 scores before treatment to 8.21 +/- 5.14 scores after treatment, with a decrement of 22.50 +/- 8.14 scores, which was higher than that in the control group, from 24.32 +/- 5.44 scores to 5.92 +/- 3.58 scores, with a decrement of 18.40 +/- 4.50 scores, the difference between them was statistically significant (t = 2.52, P = 0.014). The serum level of E2 increased in both groups significantly after treatment, from 20.23 +/- 20.78 ng/L to 54.34 +/- 24.26 ng/L in the treated group (t= -2.73, P = 0.006), and from 16.15 +/- 24.40 ng/L to 40.61 +/- 81.54 ng/L in the control group (t = -1.72, P = 0.086), but the difference between groups was statistical insignificant (t= -1.120, P = 0.263). As for levels of FSH and LH, their decrements in the control group (13.16 +/- 11.29 mlU/mL and 10.37 +/- 9.21 mlU/mL) were larger than those in the treated group (4.92 +/- 4.26 mlU/mL and 0.17 +/- 2.42 mlU/mL), respectively (t = - 2.49, P = 0.013; t = - 2.38, P = 0.017).

CONCLUSIONChinese medicinal massage manipulation could improve the Kupperman index of all the 13 symptoms in women with climacteric syndrome, and increase the E2 level in serum.

Adult ; Estradiol ; blood ; Estrogens, Conjugated (USP) ; therapeutic use ; Female ; Follicle Stimulating Hormone ; blood ; Humans ; Luteinizing Hormone ; blood ; Massage ; Menopause ; Middle Aged

Purpose To analyze the gait of patients following reconstruction of posterior cruciate ligament(PCL) and posterolateral corner(PLC).Methods Between March 2007 and April 2008,16 patients with combined PCL and PLC injuries-deficient knee underwent the reconstruction with allograft.Dial test were performed before surgery,and gait analysis and dial test were assessed 1 year after reconstruction.Sixteen healthy volunteers were used as controls.Results There are no differences in the time-distance factors and kinematics between patients and controls(P>0.05).The torque of knee extension revealed significant difference between patients and controls(P<0.01).Mean abtorsion angle of the patients increased 16.5°±6.2° before surgery;whereas the angle increased-4.4°±7.8° 1 year after surgery(P<0.01).The maximal mean abtorsion angnlation from gait analysis was 14.1°±15.7° and from dial test was 29.7°±1 5.2°,respectively.There was a strong linear correlation(r=0.9671,P<0.05)between the outcomes from dial test and gait analysis.Conclusion After reconstruction of the PCL and PLC,the gait of patients became almost normal 1 year after operation,except the torque of knee extension.

OBJECTIVE: To explore the relationship between polymorphism of apolipoprotein E (ApoE) exon 4 gene and different syndromes in patients with coronary heart disease (CHD). METHODS: Two hundred patients with CHD were divided into four groups according to syndrome differentiation, including syndrome of phlegm (PS), syndrome of blood stasis (BSS), syndrome of phlegm-blood stasis blocking (PBBS) and syndrome of non-phlegm and non-blood stasis (NPNBS). One hundred healthy volunteers were included in control group. Blood lipids were measured by routine examination. Total DNA of peripheral blood was extracted. ApoE genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. All data were analyzed by SAS software. RESULTS: (1)The occurrence rate of epsilon4 allele of ApoE in patients with CHD was 19.5%, significantly higher than 9.5% in the control group (P<0.05), and the E 3/4 genotype was especially more frequent (P<0.01). (2) The levels of total cholesterol (TC), total triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C) in patients with epsilon4 were higher than those in patients without epsilon4 (P<0.01). (3) The frequencies of epsilon4 allele and E3/4 genotype in patients with PS were significantly higher than those in patients with BSS (P<0.05). CONCLUSION: ApoE epsilon4 allele, especially E3/4 genotype, is the risk factor of CHD. There is a relatively close relationship between patients with ApoE epsilon4 allele and PS. It may be one of the main susceptible genes in CHD patients with PS.