No abstract available.
Chromosome Deletion*

BACKGROUND: Plant extracts have been widely used as important therapeutic drugs for many centuries all over the world. There have been many reports that natural products have various kinds of biological activities such as anti-allergy, anti-inflammatory and antimicrobial activities. Recently, the screening for the efficacy and safety of natural products has been extensively performed. OBJECTIVE: This study was carried out to find a beneficial plant extract possessing excellent antioxidative and anti-melanogenic activities. We have found that the leaf of Robinia pseudo-acacia L. has active substances which are involved in those activities. METHODS: To confirm the antioxidative activity of the extract obtained from the leaves of Robinia pseudo-acacia L., scavenging ability of the extract on DPPH free radicals and its inhibitory effects on lipid autoxidation and peroxidation were investigated. In addition, inhibitory effects of the extract on mushroom tyrosinase as well as melanin biosynthesis in cultured B16 melanoma cells were evaluated. RESULTS: The acacia extract showed not only powerful antioxidative activity but also antimelanogenic acitivity as strong as that of arbutin which is a well known inhibitor of melanogenesis. CONCLUSION: These resulis suggest that the extract from the leaves of Robinia pseudo-acacia L. could be used as a 4ghtening and antioxidative agent for the skin.
Acacia ; Agaricales ; Arbutin ; Biological Products ; Free Radicals ; Mass Screening ; Melanins ; Melanoma, Experimental ; Monophenol Monooxygenase ; Plant Extracts ; Plants ; Robinia* ; Skin

The characterization of genetic variation at the level of DNA has generated significant advances in gene mapping and disease diagnosis, and forensic identification of individuals. It is now possible to identify individual DNA from various tissue specimens, like hair, using the PCR and oligonucleotide probes. To date, however, the number of hairs needed, the preservation conditions, and the kinds of hair suitable for DNA extraction have not been well known. We performed DNA extraction using hairs from different body sites, using different numbers of hairs, under various different preservation conditions to investigate the acquisition conditions of DNA data from hair using PCR and specific HLA-DQA probe. HLA-DQA genotyping of DNA extracted from peripheral blood was performed to compare the results of hair and blood HLA-DQA genotyping from individuals. The results are as follows: 1) The concentration of DNA extracted from a single strand of hair is 5.23+/-0.54 g/ml. It is possible to extract sufficient DNA for HLA-DQA genotyping from a single strand of hair. 2) DNA concentration is different according to body site. Concentrations are 7.01+/-0.33 g/ml in scalp hair, 6.28+/-0.29 g/ml in axillary hair, and 6.10+0.24 microgram/ml in pubic hair. 3) There is no difference between the electrophortic bands resulting from DNA extracted from the hair of an individual preserved under different conditions, such as room temperature, exposure to sunlight, exposure to low temperature (+4degrees C), or exposure to moisture. 4) There is no difference between the electrophoretic bands resulting from DNA extracted from hair of a single individual preserved for different lengths of time. 5) In an individual, the HLA-DQA genotype obtained from peripheral blood is identical to that obtained from hair. Even though the amout of DNA obtained from hair is limited, it is possible to identify the HLA-DQA genotype of an individual using a single strand of hair. This requires adequate extraction of DNA for PCR analysis using an allele specific probe. We believe that HLA-DQA genotyping using the PCR method on DNA extracted from hair is useful for disease diagnosis and forensic science.
Alleles ; Chromosome Mapping ; Diagnosis ; DNA* ; Forensic Sciences ; Genetic Variation ; Genotype ; Hair* ; Oligonucleotide Probes ; Polymerase Chain Reaction* ; Scalp ; Sunlight

No abstract available.
Hyperthyroidism*

To Evaluate the clinical characteristics of childhood-onset systemic lupus erythemoatosus (SLE) and analyse the factors related to outcome of renal function in lupus nephritis, we reviewed medical records of 18 cases of SLF diagnosed at the Department of Pediatrics. Pusan National University Hospital from January 1981 to December 1990. The results were as follow: 1)Male to female ratio was 1:2.6. 2) Nephropathy was the most common initial impression, and 8 cases were diagnosed as SLE at first. 3) The clinical menifestation. was ANA (+), malar rash, renal disorder, hematologic disorder in frequency. 4) Diffuse proliferative lupus nephritis was the most common in renal histopathologic studies. 5) Among the follow-up cases, active lupus was 39%, remission was 28%. 6) As factors related to outcome of lupus nephrits were, hypertension and proteinuria were siginificant statistically.
Busan ; Exanthema ; Female ; Follow-Up Studies ; Humans ; Hypertension ; Lupus Erythematosus, Systemic* ; Lupus Nephritis ; Medical Records ; Pediatrics ; Proteinuria

No abstract available.

BACKGROUND: Stress is found to have influence on physical and mental disorders, and also to healthy life habits related to physical health mental stress. Job holders are groups that have suffered from chronic stressful condition. Consider medical people of job holders to received exess stress. So, we tested this investigation to know the relationship between health habits and stress amounts about nurse. METHODS: This study was performed on nurses who worked on one hosptal in Pusan Metropolitan city for one months(from April, 1, to April, 30, 2000). We used 128 results. Health habits were investigated about 5 articles out of "Breslow's 7 health habits" and stress amounts were measured by Korean translated BEPSI(Brief Encounter Psychosocial Instrument) and Bae Jong Myun's 30 items SRRQ(Social Readjustment Rating Questionnaire). RESULTS: There were differences in amounts of stress of subjects according to marital state(P<0.05), job department(P<0.05), BMI(Body Mass Index)(P<0.05), sleeping time(P<0.05). CONCLUSION: This investigation has showed that having excess stress in nurses. Body Mass Index, sleeping time appeared to be health habits related to amounts of stress.
Body Mass Index ; Busan ; Mental Disorders

Systemic lupus erythematosus (SLE), an autoimmune disease with multisystemic involvement, has been reported to be associated with a number of gastrointestinal complications. But pancreatitis is an unusual complication of SLE, occuring in only 3-4% of lupus cohort. Multiple mechanisms contributing to pancreatitis associated with SLE have been demonstrated which include vasculitis, necrotizing pancreatitis, corticosteroid administration, and vascular thrombosis. We experienced a case of pancreatitis associated with SLE in a 14-year-old girl. The cause of pancreatitis may be related secondarily to vascular thrombosis, since there were several clinical and laboratory findings suggesting thromboembolic phenomena, including false-positive test for syphilis, prologation of PT/aPTT, and pulmonary embolism.
Adolescent ; Autoimmune Diseases ; Cohort Studies ; Female ; Humans ; Lupus Erythematosus, Systemic* ; Pancreatitis* ; Pulmonary Embolism ; Syphilis ; Thrombosis ; Vasculitis

Systemic lupus erythematosus (SLE), an autoimmune disease with multisystemic involvement, has been reported to be associated with a number of gastrointestinal complications. But pancreatitis is an unusual complication of SLE, occuring in only 3-4% of lupus cohort. Multiple mechanisms contributing to pancreatitis associated with SLE have been demonstrated which include vasculitis, necrotizing pancreatitis, corticosteroid administration, and vascular thrombosis. We experienced a case of pancreatitis associated with SLE in a 14-year-old girl. The cause of pancreatitis may be related secondarily to vascular thrombosis, since there were several clinical and laboratory findings suggesting thromboembolic phenomena, including false-positive test for syphilis, prologation of PT/aPTT, and pulmonary embolism.
Adolescent ; Autoimmune Diseases ; Cohort Studies ; Female ; Humans ; Lupus Erythematosus, Systemic* ; Pancreatitis* ; Pulmonary Embolism ; Syphilis ; Thrombosis ; Vasculitis

Ischemic colitis is a common gastrointestinal disease not in childhood but in sixties decade. It's developed due to ischemic injury of colon and classified to transient reversible form, chronic form and acute fulminant form. Uniquely Authors had experienced a case of ischemic colitis diagnosed as barium enema in childhood. This patient was brought to our hospital due to abdominal pain, abdominal distension and bloody diarrhea. He was received barium enema study which showed stricture of splenic flexure of descending colon. He had severe clinical course of ischemic colitis, resulting in sepsis and acute renal failure. He was recovered from previous symptom after hemodialysis, antibiotic medication and supportive care. We saw recovery of stricture of splenic flexure by barium enema study.
Abdominal Pain ; Acute Kidney Injury ; Barium ; Child ; Colitis, Ischemic* ; Colon ; Colon, Descending ; Colon, Transverse ; Constriction, Pathologic ; Diarrhea ; Enema ; Gastrointestinal Diseases ; Humans ; Renal Dialysis ; Sepsis