1.Silibinin Enhances Ultraviolet B-Induced Apoptosis in MCF-7 Human Breast Cancer Cells.
Eun Mi NOH ; Mi Suk YI ; Hyun Jo YOUN ; Byoung Kil LEE ; Young Rae LEE ; Ji Hey HAN ; Hong Nu YU ; Jong Suk KIM ; Sung Hoo JUNG
Journal of Breast Cancer 2011;14(1):8-13
PURPOSE: Chemotherapies for breast cancer generally have strong cellular cytotoxicity and severe side effects. Thus, significant emphasis has been placed on combinations of naturally occurring chemopreventive agents. Silibinin is a major bioactive flavonolignan extracted from milk thistle with chemopreventive activity in various organs including the skin, prostate, and breast. However, the mechanism underlying the inhibitory action of silibinin in breast cancer has not been completely elucidated. Therefore, we investigated the effect of silibinin in MCF-7 human breast cancer cells and determined whether silibinin enhances ultraviolet (UV) B-induced apoptosis. METHODS: The effects of silibinin on MCF-7 cell viability were determined using the MTT assay. The effect of silibinin on PARP cleavage, as the hallmark of apoptotic cell death, and p53 protein expression in MCF-7 cells was analyzed using Western blot. The effect of silibinin on UVB-induced apoptosis in MCF-7 cells was analyzed by flow cytometry. RESULTS: A dose- and time-dependent reduction in viability was observed in MCF-7 cells treated with silibinin. Silibinin strongly induced apoptotic cell death in MCF-7 cells, and induction of apoptosis was associated with increased p53 expression. Moreover, silibinin enhanced UVB-induced apoptosis in MCF-7 cells. CONCLUSION: Silibinin induced a loss of cell viability and apoptotic cell death in MCF-7 cells. Furthermore, the combination of silibinin and UVB resulted in an additive effect on apoptosis in MCF-7 cells. These results suggest that silibinin might be an important supplemental agent for treating patients with breast cancer.
Apoptosis
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Blotting, Western
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Breast
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Breast Neoplasms
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Cell Death
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Cell Survival
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Humans
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MCF-7 Cells
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Milk Thistle
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Prostate
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Silymarin
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Skin
2.TNF-alpha upregulates PTEN via NF-kappaB signaling pathways in human leukemic cells.
Young Rae LEE ; Hong Nu YU ; Eun Mi NOH ; Hyun Jo YOUN ; Eun Kyung SONG ; Myung Kwan HAN ; Chang Sik PARK ; Byung Soo KIM ; Young Seok PARK ; Byung Kwon PARK ; Sung Ho LEE ; Jong Suk KIM
Experimental & Molecular Medicine 2007;39(1):121-127
TNF-alpha plays a variety of biological functions such as apoptosis, inflammation and immunity. PTEN also has various cellular function including cell growth, proliferation, migration and differentiation. Thus, possible relationships between the two molecules are suggested. TNF-alpha has been known to downregulate PTEN via NF-kappaB pathway in the human colon cell line, HT-29. However, here we show the opposite finding that TNF-alpha upregulates PTEN via activation of NF-kappaB in human leukemic cells. TNF-alpha increased PTEN expression at HL-60 cells in a time- and dose-dependent manner, but the response was abolished by disruption of NF-kappaB with p65 anisense phosphorothioate oligonucleotide or pyrrolidine dithiocarbamate. We found that TNF-alpha activated the NF-kappaB pathways, evidenced by the translocation of p65 to the nucleus in TNF-alpha-treated cells. We conclude that TNF-alpha induces upregulation of PTEN expression through NF-kappaB activation in human leukemic cells.
Up-Regulation/*drug effects
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Tumor Necrosis Factor-alpha/*pharmacology
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Signal Transduction/*drug effects
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PTEN Phosphohydrolase/genetics/*metabolism
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NF-kappa B/genetics/*metabolism
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Leukemia/genetics/*metabolism
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Humans
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Gene Expression
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Cell Line, Tumor
3.PET-CT Manifestation and Clinical Analysis of Waldenstrom Macroglobulinemia.
Chong-Yang DING ; Hong-Yu LIU ; Zhe GUO ; Tian-Nu LI
Journal of Experimental Hematology 2016;24(6):1782-1786
OBJECTIVETo investigate the PET-CT manifestation and clinical features of patients with Waldenstrom macroglobulinemia(WM) .
METHODSThe clinical features, laboratorial examination results and PET-CT manifestation of 12 patients with WM were analyzed retrospectively.
RESULTSThe average age of 12 patients with WM was 63.5 years old, the most common incipient symptoms were fatigue and hyperviscosity syndrome. The median SUVof bone marrow was 4.9 (range of 2.1 to 21.9), with diffusely increasedF-FDG uptake in all the patients. In 6 patients, the median SUVof splenomegaly and lymphadenectasis was 3.2 (range of 2.3 to 5.2) and 5.2 (range of 3.6 to 11.2), respectively. The SUVof bone marrow, splenomegaly and lymphadenectasis did not related with white blood cell count, hemoglobin level, blood platelet count, β2- microglobulin level and serum monoclonal IgM of such patients, respectively (both P>0.5). In univariate analysis, serum monoclonal IgM and the SUVof lymphadenectasis were found to be the prognostic factors (both P<0.5).
CONCLUSIONTheF-FDG PET-CT feature of Waldenstrom macroglobulinemia has some characteristics which are helpful for setting up the clinical therapeutic schedule.
4. Adolescent stress increases depression-like behaviors and alters the excitatory-inhibitory balance in aged mice
Hong-Li WANG ; Ya-Xin SUN ; Xiao LIU ; Han WANG ; Yu-Nu MA ; Yun-Ai SU ; Ji-Tao LI ; Tian-Mei SI
Chinese Medical Journal 2019;132(14):1689-1699
Background:
Depression affects approximately 5% of elderly people and its etiology might be related to chronic stress exposure during neurodevelopmental periods. In this study, we examined the effects of adolescent chronic social stress in aged mice on depressive behaviors and the excitatory-inhibitory (E/I) balance in stress-sensitive regions of the brain.
Methods:
Sixty-four adolescent, male C57BL/6 mice were randomly assigned to either the 7-week (from post-natal days 29 to 77) social instability stress (stress group,
5.Expression Level and Target Gene Prediction of miR-181b in Patients with Chronic Lymphocytic Leukemia.
Zhen KOU ; Hong LIU ; Yi-Chun WANG ; Qin HUANG ; Zeng-Sheng WANG ; Zai-Li Nu Er GU ; Tao LANG ; Yu-Ling NIE ; Li AN ; Zi-Gu Li A ; He-Ta Bai Er MU ; Xiao-Yan ZHANG ; Ling FU ; He-Mai Jiang AI ; Min MAO ; Xiao-Min WANG ; Yan LI
Journal of Experimental Hematology 2020;28(3):808-814
OBJECTIVE:
To investigate the expression level of miR-181b in CD19+ B lymphocytes of patients with chronic lymphocytic leukemia (CLL), to analyze the relationship between its expression and the prognosis of CLL patients, and to predict the potential target gene of miR-181b in CLL by using bioinformatics.
METHODS:
Eight-four patients with CLL treated in People's Hospital of Xinjiang Uygur Autonomous Region from June 2013 to June 2018 were selected. and 20 healthy people were selected as control group. RNA was extracted from CD19+B lymphocytes of peripheral blood by magnetic bead sorting, the expression level of miR-181b was detected, and it's expression differences in different IPI groups were analyzed. The correlation between the expression level of miR-181b and PFS of CLL patients also was analyzed. miR-181b target genes were predicted by online database and literatures, and gene annotation analysis and relevant signal pathway analysis were performed for candidate target genes.
RESULTS:
The expression level of miR-181b in CLL patients was significantly lower than that in control group (P<0.01); The expression level of miR-181b in the low-risk group was higher than that in high-risk group and extremely high-risk group (P<0.05), but there was no statistical difference between low-risk group and medium-risk group (P=1.00). The expression level of miR-181b in medium-risk group was higher than that in high-risk group and extremely high-risk group (P<0.05), but there was no difference between high-risk group and extremely high-risk group (P=1.00). ROC curve results showed that the area under the curve (AUC) was 0.792 (P<0.01).When the expression level of miR-181b was at the threshold value of 0.279, it showed a better sensitivity (62.9%) and specificity (91.8%). Survival analysis results suggested that compared with the high expression group, the miR-181b low expression group had poor PFS (log rank: P=0.047). Prediction of miR-181b by using the starBase, targetscan and picTar database and its combination with literature reports indicated that CARD11, ZFP36L1, RUNX1, NR4A3, ATP1B1, PUM1 and PLAG1 related with blood diseases, and up-regulated CARD11 and ZFP36L1 participated in lymphoid tumor formation by promoting cell proliferation and inhibiting cell aging.
CONCLUSION
The expression level of miR-181b in CLL group are significantly lower than that in the controls group, and the low expression of miR-181b relates with poor prognosis of CLL patients. Through bioinformatics prediction and combined with literature reports, it is speculated that CARD11 and ZFP36L1 as target genes of miR-181b may be participated in the occurrence and development of CLL. Further experiments are needed to verify this result.
Apoptosis Regulatory Proteins
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Cell Proliferation
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Humans
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Leukemia, Lymphocytic, Chronic, B-Cell
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genetics
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MicroRNAs
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Prognosis
6.The Nucleus Accumbens CRH-CRHR1 System Mediates Early-Life Stress-Induced Sleep Disturbance and Dendritic Atrophy in the Adult Mouse.
Ting WANG ; Yu-Nu MA ; Chen-Chen ZHANG ; Xiao LIU ; Ya-Xin SUN ; Hong-Li WANG ; Han WANG ; Yu-Heng ZHONG ; Yun-Ai SU ; Ji-Tao LI ; Tian-Mei SI
Neuroscience Bulletin 2023;39(1):41-56
Adverse experiences in early life have long-lasting negative impacts on behavior and the brain in adulthood, one of which is sleep disturbance. As the corticotropin-releasing hormone (CRH)-corticotropin-releasing hormone receptor 1 (CRHR1) system and nucleus accumbens (NAc) play important roles in both stress responses and sleep-wake regulation, in this study we investigated whether the NAc CRH-CRHR1 system mediates early-life stress-induced abnormalities in sleep-wake behavior in adult mice. Using the limited nesting and bedding material paradigm from postnatal days 2 to 9, we found that early-life stress disrupted sleep-wake behaviors during adulthood, including increased wakefulness and decreased non-rapid eye movement (NREM) sleep time during the dark period and increased rapid eye movement (REM) sleep time during the light period. The stress-induced sleep disturbances were accompanied by dendritic atrophy in the NAc and both were largely reversed by daily systemic administration of the CRHR1 antagonist antalarmin during stress exposure. Importantly, Crh overexpression in the NAc reproduced the effects of early-life stress on sleep-wake behavior and NAc morphology, whereas NAc Crhr1 knockdown reversed these effects (including increased wakefulness and reduced NREM sleep in the dark period and NAc dendritic atrophy). Together, our findings demonstrate the negative influence of early-life stress on sleep architecture and the structural plasticity of the NAc, and highlight the critical role of the NAc CRH-CRHR1 system in modulating these negative outcomes evoked by early-life stress.
Animals
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Mice
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Corticotropin-Releasing Hormone/metabolism*
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Nucleus Accumbens/metabolism*
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Receptors, Corticotropin-Releasing Hormone/metabolism*
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Sleep
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Sleep Wake Disorders
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Stress, Psychological/complications*