This study aims to develop an abdominal acupoint localization system based on computer vision and convolutional neural networks (CNNs). To address the challenge of abdominal acupoint localization, a multi-task CNNs architecture was constructed and trained to locate the Shenque (CV8) and human body boundaries. Based on the identified Shenque (CV8), the system further deduces key characteristics of four acupoints: Shangwan (CV13), Qugu (CV2), and bilateral Daheng (SP15). An affine transformation matrix is applied to accurately map image coordinates to an acupoint template space, achieving precise localization of abdominal acupoints. Testing has verified that this system can accurately identify and locate abdominal acupoints in images. The development of this localization system provides technical support for TCM remote education, diagnostic assistance, and advanced TCM equipment, such as intelligent acupuncture robots, facilitating the standardization and intelligent advancement of acupuncture.
Acupuncture Points ; Humans ; Deep Learning ; Abdomen/diagnostic imaging* ; Neural Networks, Computer ; Acupuncture Therapy ; Image Processing, Computer-Assisted

Metachromatic leukodystrophy (MLD) is an inherited disease caused by a deficiency of the enzyme arylsulfatase A (ARSA). Lentivirus-modified autologous hematopoietic stem cell gene therapy (HSCGT) has recently been approved for clinical use in pre and early symptomatic children with MLD to increase ARSA activity. Unfortunately, this advanced therapy is not available for most patients with MLD who have progressed to more advanced symptomatic stages at diagnosis. Patients with late-onset juvenile MLD typically present with a slower neurological progression of symptoms and represent a significant burden to the economy and healthcare system, whereas those with early onset infantile MLD die within a few years of symptom onset. We conducted a pilot study to determine the safety and benefit of HSCGT in patients with postsymptomatic juvenile MLD and report preliminary results. The safety profile of HSCGT was favorable in this long-term follow-up over 9 years. The most common adverse events (AEs) within 2 months of HSCGT were related to busulfan conditioning, and all AEs resolved. No HSCGT-related AEs and no evidence of distorted hematopoietic differentiation during long-term follow-up for up to 9.6 years. Importantly, to date, patients have maintained remarkably improved ARSA activity with a stable disease state, including increased Functional Independence Measure (FIM) score and decreased magnetic resonance imaging (MRI) lesion score. This long-term follow-up pilot study suggests that HSCGT is safe and provides clinical benefit to patients with postsymptomatic juvenile MLD.
Humans ; Leukodystrophy, Metachromatic/genetics* ; Pilot Projects ; Genetic Therapy/methods* ; Hematopoietic Stem Cell Transplantation ; Male ; Follow-Up Studies ; Female ; Lentivirus/genetics* ; Child ; Child, Preschool ; Hematopoietic Stem Cells/metabolism* ; Cerebroside-Sulfatase/metabolism* ; Adolescent

Objective: This study is the first to adopt a cultural and contextual coping model to examine the impact of forbearance coping on compliance and psychological health and unravel the psychosocial mediator and moderator among community-dwelling residents in the post-pandemic era in China. Methods: A multistage cluster convenience sampling strategy was conducted to recruit 402 community-dwelling residents of ten cities in Guangdong province who completed an online survey measuring forbearance coping, anti-pandemic compliance behaviours, family resilience, community resilience and psychological distress. Results: The study results showed an unexpected negative influence of forbearance coping on psychological distress. Nevertheless, forbearance coping facilitated personal compliance with coronavirus disease-2019 mitigation measures and improved psychological health. More importantly, meaning-making of adversity within families partially mediated the negative effect of forbearance on mental health only when respondents perceived higher levels of community resilience. Conclusion This study is the first to apply the cultural and contextual model of coping to Chinese individuals during a significant public health crisis. It expands the model by uncovering mechanisms like behavioural compliance and family meaning-making, and highlights the moderating role of community resilience. The findings emphasize the importance of building resilient communities and supporting mental health, providing evidence for future policy interventions and pandemic/disaster prevention measures in China.

Objective: This study is the first to adopt a cultural and contextual coping model to examine the impact of forbearance coping on compliance and psychological health and unravel the psychosocial mediator and moderator among community-dwelling residents in the post-pandemic era in China. Methods: A multistage cluster convenience sampling strategy was conducted to recruit 402 community-dwelling residents of ten cities in Guangdong province who completed an online survey measuring forbearance coping, anti-pandemic compliance behaviours, family resilience, community resilience and psychological distress. Results: The study results showed an unexpected negative influence of forbearance coping on psychological distress. Nevertheless, forbearance coping facilitated personal compliance with coronavirus disease-2019 mitigation measures and improved psychological health. More importantly, meaning-making of adversity within families partially mediated the negative effect of forbearance on mental health only when respondents perceived higher levels of community resilience. Conclusion This study is the first to apply the cultural and contextual model of coping to Chinese individuals during a significant public health crisis. It expands the model by uncovering mechanisms like behavioural compliance and family meaning-making, and highlights the moderating role of community resilience. The findings emphasize the importance of building resilient communities and supporting mental health, providing evidence for future policy interventions and pandemic/disaster prevention measures in China.

Objective: This study is the first to adopt a cultural and contextual coping model to examine the impact of forbearance coping on compliance and psychological health and unravel the psychosocial mediator and moderator among community-dwelling residents in the post-pandemic era in China. Methods: A multistage cluster convenience sampling strategy was conducted to recruit 402 community-dwelling residents of ten cities in Guangdong province who completed an online survey measuring forbearance coping, anti-pandemic compliance behaviours, family resilience, community resilience and psychological distress. Results: The study results showed an unexpected negative influence of forbearance coping on psychological distress. Nevertheless, forbearance coping facilitated personal compliance with coronavirus disease-2019 mitigation measures and improved psychological health. More importantly, meaning-making of adversity within families partially mediated the negative effect of forbearance on mental health only when respondents perceived higher levels of community resilience. Conclusion This study is the first to apply the cultural and contextual model of coping to Chinese individuals during a significant public health crisis. It expands the model by uncovering mechanisms like behavioural compliance and family meaning-making, and highlights the moderating role of community resilience. The findings emphasize the importance of building resilient communities and supporting mental health, providing evidence for future policy interventions and pandemic/disaster prevention measures in China.

Objective: This study is the first to adopt a cultural and contextual coping model to examine the impact of forbearance coping on compliance and psychological health and unravel the psychosocial mediator and moderator among community-dwelling residents in the post-pandemic era in China. Methods: A multistage cluster convenience sampling strategy was conducted to recruit 402 community-dwelling residents of ten cities in Guangdong province who completed an online survey measuring forbearance coping, anti-pandemic compliance behaviours, family resilience, community resilience and psychological distress. Results: The study results showed an unexpected negative influence of forbearance coping on psychological distress. Nevertheless, forbearance coping facilitated personal compliance with coronavirus disease-2019 mitigation measures and improved psychological health. More importantly, meaning-making of adversity within families partially mediated the negative effect of forbearance on mental health only when respondents perceived higher levels of community resilience. Conclusion This study is the first to apply the cultural and contextual model of coping to Chinese individuals during a significant public health crisis. It expands the model by uncovering mechanisms like behavioural compliance and family meaning-making, and highlights the moderating role of community resilience. The findings emphasize the importance of building resilient communities and supporting mental health, providing evidence for future policy interventions and pandemic/disaster prevention measures in China.

Objective: This study is the first to adopt a cultural and contextual coping model to examine the impact of forbearance coping on compliance and psychological health and unravel the psychosocial mediator and moderator among community-dwelling residents in the post-pandemic era in China. Methods: A multistage cluster convenience sampling strategy was conducted to recruit 402 community-dwelling residents of ten cities in Guangdong province who completed an online survey measuring forbearance coping, anti-pandemic compliance behaviours, family resilience, community resilience and psychological distress. Results: The study results showed an unexpected negative influence of forbearance coping on psychological distress. Nevertheless, forbearance coping facilitated personal compliance with coronavirus disease-2019 mitigation measures and improved psychological health. More importantly, meaning-making of adversity within families partially mediated the negative effect of forbearance on mental health only when respondents perceived higher levels of community resilience. Conclusion This study is the first to apply the cultural and contextual model of coping to Chinese individuals during a significant public health crisis. It expands the model by uncovering mechanisms like behavioural compliance and family meaning-making, and highlights the moderating role of community resilience. The findings emphasize the importance of building resilient communities and supporting mental health, providing evidence for future policy interventions and pandemic/disaster prevention measures in China.

Cancer stands as one of the predominant causes of mortality globally, necessitating ongoing efforts to develop innovative therapeutics. Historically, natural products have been foundational in the quest for anticancer agents. Bulbocodin D (BD) and Bulbocodin C (BC), two bibenzyls derived from Pleione bulbocodioides (Franch.) Rolfe, have demonstrated notable in vitro anticancer activity. In human lung cancer A549 cells, the IC_50s for BD and BC were 11.63 and 11.71 μmol·L^-1, respectively. BD triggered apoptosis, as evidenced by an upsurge in Annexin V-positive cells and elevated protein expression of cleaved-PARP in cancer cells. Furthermore, BD and BC markedly inhibited the migratory and invasive potentials of A549 cells. The altered genes identified through RNA-sequencing analysis were integrated into the CMap dataset, suggesting BD's role as a potential signal transducer and activator of transcription 3 (STAT3) inhibitor. SwissDock and MOE analyses further revealed that both BD and BC exhibited a commendable binding affinity with STAT3. Additionally, a surface plasmon resonance assay confirmed the direct binding affinity between these compounds and STAT3. Notably, treatment with either BD or BC led to a significant reduction in p-STAT3 (Tyr 705) protein levels, regardless of interleukin-6 stimulation in A549 cells. In addition, the extracellular signal-regulated kinase (ERK) was activated after BD or BC treatment. An enhancement in cancer cell mortality was observed upon combined treatment of BD and U0126, the MEK1/2 inhibitor. In conclusion, BD and BC emerge as promising novel STAT3 inhibitors with potential implications in cancer therapy.
Humans ; Lung Neoplasms/metabolism* ; STAT3 Transcription Factor/metabolism* ; Antineoplastic Agents/chemistry* ; A549 Cells ; Apoptosis ; Cell Line, Tumor ; Cell Proliferation

OBJECTIVE: To explore the relationship between drug treatment and outcomes in patients with late-onset severe pneumonia (LOSP) after allogeneic stem cell transplantation (allo-SCT). METHODS: We retrospectively analyzed the effects of the initiation time of treatment drugs, especially antiviral drugs and glucocorticoids on the clinical outcomes in 82 patients between January 2016 and August 2021 who developed LOSP after allo-SCT in Peking University People's Hospital. Univariate analysis was performed by Mann-Whitney U test and χ² test, and multivariate analysis was performed by Logistic regression. When multiple groups (n>2) were involved in the χ² test, Bonferroni correction was used for the level of significance test. RESULTS: Of all 82 patients in this study, the median onset time of LOSP was 220 d (93-813 d) after transplantation, and the 60-day survival rate was 58.5% (48/82). The median improvement time of the survival patients was 18 d (7-44 d), while the median death time of the died patients was 22 d (2-53 d). Multivariate analysis showed that the initiation time of antiviral drugs from the onset of LOSP (< 10 d vs. ≥10 d, P=0.012), and the initiation time of glucocorticoids from antiviral drugs (< 10 d vs. ≥10 d, P=0.027) were the factors affecting the final outcome of the patients with LOSP at the end of 60 d. According to the above results, LOSP patients were divided into four subgroups: group A (antiviral drugs < 10 d, glucocorticoids ≥10 d), group B (antiviral drugs < 10 d, glucocorticoids < 10 d), group C (antiviral drugs ≥10 d, glucocorticoids ≥10 d) and group D (antiviral drugs ≥10 d, glucocorticoids < 10 d), the 60-day survival rates were 91.7%, 56.8%, 50.0% and 21.4%, respectively. CONCLUSION Our study demonstrated that in patients who developed LOSP after allo-SCT, the initiation time of antiviral drugs and glucocorticoids were associated with the prognosis of LOSP, and the survival rate was highest in patients who received antiviral drugs early and glucocorticoids later. It suggested that for patients with LOSP of unknown etiology should be highly suspicious of the possibility of a secondary hyperimmune response to viral infection.
Antiviral Agents/therapeutic use* ; Glucocorticoids/therapeutic use* ; Hematopoietic Stem Cell Transplantation/methods* ; Humans ; Pneumonia/etiology* ; Prognosis ; Retrospective Studies ; Transplantation, Homologous/adverse effects*

OBJECTIVE: In order to conduct high-throughput genome-wide translocation sequencing based on CRISPR/Cas9, Nalm6-cas9 monoclonal cell line expressing Cas9 protein was constructed by lentivirus transduction. METHODS: Lentiviral vectors LentiCas9-Blast, pSPAX2, and pMD2.G were used to co-transfect HEK293T cells to obtain recombinant lentivirus. After Nalm6 cells were infected with the recombinant lentivirus, the cells were screened by Blasticidin, and multiple monoclonal cell lines expressing Cas9 protein were obtained by limited dilution. Western blot was used to detect the expression level of Cas9 protein in monoclonal cell lines, and cell count analysis was used to detect the proliferation activity of monoclonal cell lines. LentiCRISPRV2GFP-Δcas9, LentiCRISPRV2GFP-Δcas9-AF4, LentiCRISPRV2GFP-Δ cas9-MLL plasmids were constructed, and transfected with pSPAX2 and pMD2.G, respectively. T vector cloning was used to detect the function of Cas9 protein in Nalm6-Cas9 monoclonal cell line infected with virus. RESULTS: Western blot showed that Nalm6-Cas9_1-6 monoclonal cell line had high expression of Cas9 protein. Cell count analysis showed that high expression of Cas9 protein in Nalm6-Cas9_1-6 monoclonal cell line did not affect cell proliferation activity. The Nalm6-Cas9_1-6 monoclonal cell line had high cleavage activity, and the editing efficiency of AF4 and MLL genes was more than 90% which was determined by T vector cloning. CONCLUSION Nalm6-Cas9_1-6 monoclonal cell line stably expressing highly active Cas9 protein was obtained, which provided a basis for exploring the translocation of MLL in therapy-related leukemias based on CRISPR/Cas9 genome-wide high-throughput genome-wide translocation sequencing.
CRISPR-Associated Protein 9/genetics* ; CRISPR-Cas Systems ; Genetic Vectors ; HEK293 Cells ; Humans ; Lentivirus/genetics* ; Plasmids