Adult ; Dry Eye Syndromes ; Female ; Humans ; Male ; Occupational Diseases ; Welding ; Young Adult

Objective　To show the condition that the NOSIE (Nurses' Observation Scale for Inpatient Evaluation) used in schizophrenia patients. Method　 To evaluate the male and female schizophrenia patients (paranoid type) by NOSIE. Results　 The total cases were 100 patients, male and female were both 50 cases. The result of evaluation showed that there was significant difference in 5 factors such as social competence, irritability, retardation, psychosis and depressive between male and female patients. As well, it matched with clinical information. The result of the consistency test for survey indicated that its Pearson coefficient of correlation for all of 7 factors in NOSIE were 0.617～0.843. Conclusion NOSICE can provided some objective data to clinical works of nurses. But, in other hand, its identity is lower.

BACKGROUND: We can investigate mechanism of endogenous neuroprotection in rat cerebral hypoxic tolerance trial. OBJECTIVE: To observe the characteristics of cerebral hypoxic tolerance in rat models with cerebral hypoxic preconditioning. DESIGN: Randomized controlled observation. SETTING: Department of Neurology, China-Japanese Friendship Hospital, Jilin University. MATERIALS: The experiment was performed in the Basic Animal Experimental Center, China-Japan Friendship Hospital, Jilin University from April 2003 to April 2004. Inbred line healthy Wistar rats, of either sex, with the body mass of 200-300 g, were randomly assigned into normal control group (n=6), sham operation group (n=6), ischemic control group (n=20), hypoxic preconditioning (3 hours, 8% O2 and 92% N2) plus ischemic group (n=60) (according to different hypoxic phases, there were 5 time phases: 30 minutes, 1, 3, 5 and 6 hours with 12 rats in each time phase), hypoxic preconditioning group (n=18) [according to different hypoxic phases, there were 3 time phases: 1, 3 and 5 hours with 6 rats in each time phase, 3 rats received TTC staining and 3 rats received hematoxylin and eosin (HE) staining]. METHODS: ①Hypoxic preconditioning: Firstly, natrica calx was put into closed glass container to absorb CO2 and O2, secondly, mixed gas of 8% O2 and 92% N2 was input, and then animals were put into the container, 3 rats each time. Temperature and humidity were kept steadily. ②Permanent ischemic middle cerebral artery rat models were established. ③The models were determined with a series in procedures: neurological score, infarcted volume evaluation, pathological sample preparation, immunohistochemical staining, imaging analysis and so on. ④The data were compared in groups with variance analysis.MAIN OUTCOME MEASURES: Changes in cerebral infarcted volume, neurological score and pathological morphology in rats of experimental group and control group. RESULTS: Neurological score in the hypoxic preconditioning (8% O2, at hours 1, 3 and 5) plus ischemic group was lower than in the ischemic control group(P＜0.01). Neurological score at minute 30 and hour 6 after hypoxia (8% O2) had insignificant difference in the ischemic control group. Mean cerebral infarcted volume ratio in the hypoxic preconditioning (8% O2, at hours 1, 3 and 5) plus ischemic group was lower than in the ischemic control group(P＜0.01). Mean cerebral infarcted volume ratio after hypoxia (8% O2, at minute 30 and hour 6) had insignificant difference with ischemic control group (P＞0.05). CONCLUSION: Hypoxic preconditioning in rats can effectively release nerve injury induced by focal cerebral ischemia, suggesting that it has protective effect on brain. The procedure of establishing cerebral ischemic tolerance models with hypoxic preconditioning, which is simple and stable, with little injury on experimental animals, is a useful tool for studying cerebral ischemic tolerance.

Adolescent ; Dentigerous Cyst ; pathology ; Humans ; Jaw Cysts ; pathology ; Male

Catheter Ablation ; methods ; Child ; Epicardial Mapping ; Female ; Heart Aneurysm ; complications ; surgery ; Humans ; Tachycardia, Ventricular ; complications ; surgery

Streptozotocin-induced diabetic rats were treated with GSH for 12 weeks.The results showed that GSH significantly improved the expressions of NF-KB and inducible nitrie oxide synthase and ameliorated the myocardial tissue injury.

With the development of functional genomics, high throughput analysis of genes’ function has been the mainstream of research, and exogenous gene's over expression via Agrobacterium-mediated transformation is the most commonly used method in gene functional analysis.The versatile plant expression vector cassette named pBHT-5 was constructed by the method of site-specific mutagenesis based on pBI121. First of all, the restriction enzyme SfiI recognition site in trfA gene (X00713) which was relevant to plasmid replication and stability was replaced without changing its amino acid composition. And then the SfiIA,SfiIB sites were added between promoter CaMV35s and terminator NOS. The versatile plant expression vector cassette can be directly used to construct plant expression vector containing the full-length genes cloned by Clontech SMARTTM technology, which will raise the efficiency of vector construction. The result will provide basis of new genes’ high throughput screening and functional analysis, then get the new genes functioning in plant osmotic stress resistance.

Objective To evaluate the value of diffusion-weighted imaging (DWI)in diagnosing the acute centrum ovale infarction, and also to investigate the pathogenesis of the infarction. Methods All 58 patients underwent conventional MRI and DWI scanning after symptoms’ onset. DWI findings were compared to the findings of T_1WI and T_2WI. Results The sensitivity and specificity in diagnosing the ischemia stroke were 96.4% and 98.8% within 7 days after onset. Of all the cases, 62.1% were associated with the cerebral large-vessel disease and emboligenic heart disease. Only 36.2% had a classic lacunar syndrome but 69.0% had more frequently an abrupt onset of symptoms. Conclusion DWI is of high accuracy for diagnosing centrum ovale infarction and detecting early infarction lesions which are difficult to be displayed in conventional MRI, and very helpful in differentiating the acute from non-acute lesions; symptomatic centrum ovale infarction is suggested to be associated with large-vessel and heart disease which should be distinguished from the lacunar infarcts.

Objective To prepare a 10-hydroxycamptothecin (10-HCPT) loaded folate-receptor targeted phase-change contrast agent (FR-HCPT-PNPCA),and to study the general characteristics including drug loading,phase changing and targeting capability in vitro.Methods Using a method of two-step emulsification,the phase-change nanoparticles loading anticancer drug (10-HCPT) with lipids shell and liquid pefluorocarbon core were prepared.The entrapment efficiency and the drug-loading amounts were studied by high performance liquid chromatography,and the phase transition of the nanoparticles after heating was observed.The targeting ability was evaluated on liver cancer cell line 7721 in vitro.Results The FR-HCPT-PNPCA,with a drug encapsulation rate of about 70.42 ％ and drug loading amounts of about 20.05 ％,was prepared successfully.When being heated to 70℃,obvious phase changing and microbubbles generating could be observed under microscope.In addition,a large amount of FR-HCPT-PNPCA particles could adhere specifically around the 7721 cells.Conclusion The prepared FR-HCPT-PNPCA,which has a stable characteristic and high performance of drug loading and tumor targeting,is expected to become a promising multifunctional molecular ultrasound probe for diagnosis and treatment of tumor.

Abstract? AlM: To observe the effect of combining partial reduction orthokeratology ( Ortho-K ) and spectacles on slowing myopic progressionin high myopic adolescent.? METHODS: Sixty - nine eyes of 36 high myopic adolescent ( aged 9 ~15 years ) with spherical equivalent refraction ≧-6. 00 diopters ( D) ( spherical component≧-5. 50D) were fitted with custom-made four-zone/five-curve Ortho-K lenses. The target of reduction was to achieve -5. 00D for both eyes. The residual refractive errors after at least one month of Ortho-K wear were corrected with single-vision spectacles for clear vision in the daytime. The unaided visual acuity ( UVA) , refractive error ( RE ) , axial length ( AL ) , and ocular health were assessed before the Ortho-K lens wear, and followed up for 2a after Ortho-K.?RESULTS: ( 1 ) Changes in UVA: The mean UVA was 0. 09±0. 05 at baseline before Ortho-K;the mean UVA was 0. 27 ± 0. 14, 0. 54 ± 0. 18, 0. 78 ± 0. 24, and 0. 81 ± 0. 19, respectively after Ortho-K wear for l night, 1wk, 1, and 3mo. The differences of UVA were significant with baseline (P<0. 05), and became stable 1mo after the treatment. (2) Changes in RE:The mean RE was -6. 82± 0. 71D at baseline before Ortho-K and -6. 86 ± 0. 77D after Ortho-K wear for 1a (P>0. 05 compared to baseline). The mean RE was-7. 11±0. 81D after Ortho-K wear for 2a, and the amount of myopia increased -0. 29 ± 0. 37D compared to baseline (P<0. 05). (3) Changes in AL: The mean AL was 26. 18 ± 0. 57mm at baseline before Ortho-K, and it was not significantly different (P>0. 05) from the AL after Ortho-K wear for 6mo (26. 19±0. 54mm) and for 1a (26. 21± 0. 47mm). The AL was 26. 37±0. 59mm after Ortho-K wear for 2a, and the mean increase was 0. 19 ± 0. 28mm compared to baseline (P<0. 05). (4) Grade 1 corneal staining was observed in some subjects at each visit. However, the staining was improved after lens cleaning, discontinuing lens wear, or applying artificial tears. No other adverse events were reported in all subjects during the 2a study.?CONCLUSlON:Combining partial reduction Ortho-K and spectacles completely slowed myopic progression in high myopic adolescent after receiving the treatment for 1a, and partially reduced myopia progression after 2a of treatment. No severe ocular complications were found throughout the treatment. The combination treatment appeared to be effective and safe, but its long-term effect needs to be further assessed.