The technique of transcervical chorionic villus sampling (TCVS) was studied in 155 cases for selected abortion in the first trimester of pregnancy, and 3 kinds of suction tube were compared. The ultrasonic mothod for the aspiration of chorionie villus sampling is more effective than the blind aspiration, giving a success rate of 99%. The modified suction tube with a stainless steel wire along-side as a supporter is simpler than the Portex's tube, giving a oneshot success rate of 85%. This shows that the TCVS is more satisfactory if the embryo is implanted into the posterior wall of the uterus. With the B-mode scanner, it gives the highest one-shot success rate and the amount of the obtained tissue is the greatest in the 7th and 8th weeks of gestation. Twenty cases were followed up for 3 to 7 days after the procedure before selective abortion. No complications occurred during and after the TCVS, indicating that this technique ia simple, safe and reliable.

Mother-Fetus ABO blood group incompatibility is one of the most common causes of hemolytic disease of newborns in China. To identify the blood group of the fetus in case of suspected mother-fetus blood group incompatibility in the first trimester of pregnancy, the author determined blood group substance (BGS) in 40 chorionic villi samples taken by the absorption-elution procedure during 6 to 12 weeks of gestation, with a simultaneous parental blood group determination as control. The BGS detected in those specimens were all in accordance with those of the blood groups of their coriesponding parents. Among the 40 samples of chorionic villi, 20 were redetermined, and the results were the same. This demonstrates the feasibility of the procedure to identify the fetal blood groups by the determination of BGS in the chorionic villi by transcervical aspiration. The study presents, apparently, a new and simple diagnostic method for detecting mother-fetus blood incompatibility in early pregnancy, thas giviing a prospectively preventive study on those types of blood group incompatibility.

Objective To investigate the effect of extracapsular cataract extration by small incision after glaucomatous filtering surgery.Methods Non-phacoemulsificatinon extracapsular cataract extraction with posterior chamber intraocular lens implantation through temperal small tunnel incision was performed on 56 eyes of 48 glaucoma patients with preexisting filtering bleb.Results Visual acuity was improved in 56 eyes of 48 cases postoperatively.No severe complication was found intraoperatively and postoperatively.The postoperative IOP was (14.18?3.72)mm Hg.The functiond filtering bled had no apparently cicatrisation.Conclusion The operation is an effective method to treat cataract after glauconcatous filtering surgery.

Objective]Observe the clinical effect of early herpes zoster which were treated by venesection ventouse combined with helium-neon laser and drugs. [Methods]The control group 32 cases used Famciclovir Tablet,transfer factor capsules,mecobalamin and acyclovir cream-drug for external use. While the experimental group 32 cases used Famciclovir Tablet,transfer factor capsules,mecobalamin and acyclovir cream-drug for external use. While the experimental group besides taking above-medicine, were treated with venesection ventouse and helium-neon laser at the pathological change. [Results]The control group used to cure for an average of 13 ±2.5 days. The experimental group used to cure for an average of 9.6 ±2.4 days. There were significant differences(P<0.05). [Conclusion] Early herpes zoster treated by venesection ventouse combined with helium-neon laser and drugs, can significantly shorten the duration of symptoms, alleviate suffering, and has good clinical efficacy.

AIM: To investigate the effect of prostanoid DP receptors (DPR) on sleep-wake regulation in mice. METHODS: Under pentobarbital anesthesia, mice were chronically implanted with electroencephalogram (EEG) and electromyogram (EMG) electrodes for polysomnographic recordings. The spontaneous sleep-wake cycles were monitored continuously by EEG/EMG recording system for 24 h beginning at 800 p.m. and analyzed by SLEEPSIGN software in DPR knock out (KO) and wild type (WT) mice. RESULTS: DPR-KO mice exhibited a similar circadian rhythm of sleep-wake cycles to WT mice. The amounts of rapid eye movement (REM) sleep or non-REM (NREM) sleep during both the light and dark periods were identical between the DPR-KO and WT mice. Whereas, an increase in the episode number of wakefulness and a shortage in the duration of NREM sleep were found in DPR-KO mice during the light period compared with WT mice. Moreover, DPR-KO mice showed lower activity in delta-wave component in NREM sleep and higher activity in theta-wave component in REM sleep than WT mice. CONCLUSION: DPR plays a crucial role in mediating the prostaglandin D2-induced sleep. Deficiency of DPR results in the low intensity and fragmented diurnal NREM sleep and the high vigilance REM sleep, with the normal circadian rhythm of sleep in mice.

Silk fiber,as a kind of natural polymer,has been used as surgical sutures in clinic for years.Silk fiber is composed of a filament core protein,termed fibroin and a glue-like coating called sericin proteins.Due to its unique mechanical properties,good biocompatibility and slow degradability,silk fiber has been put in variety of applications as a new kind of biomedical scaffolds in recent years.With the development of the processing technique,silk fiber can be processed through the new versatile processing method to form various forms of scaffolds and its surface could be modified for different purposes.Further more,it could be tailored through genetic recombination to form the silk fibroin-like polymers which has a promising potential in medical applications.

The ras-mitogen activeted protein kinase pathway is responsible for regulation of many cel-lular processes, including differentiation, cytoskeletal organization, and protein trafficking. Much attention has been paid to them and their role in cancer therapy recently, and its inhibitor pathways are applied to clinical tes-ting, such as, Raf kinse inhibitors, MEK inhibitor, Src kinase inhibitor.

Objective:To explore the depressive symptom of nurses and its relationship with trait coping style,role perceive and personal control.Methods:425 nurses in the 2nd Hangzhou general hospital completed a battery of questionnaires consisted of part items of SCL-90,Trait Coping Style Questionnaire(TCSQ) and Role Perceive and Personal Control Scale.Results:There are significant correlation among the SCL-90 score,the coping styles,and the control feeling and role conflict.Based on depression factor ,factors of negative coping style,control feeling,role conflict and positive coping style were included in stepwise regression equation.Conclusion:Depressive symptom of nurses was related to trait coping style,role perceive and personal control.

Objective:To investigate the relationship among college student’s depression symptoms, problem solving appraisal and perceived stress.Methods:275 college students completed questionnaires, including CES-D, Chinese Perceived Stress Scale (CPSS) and Chinese Problem Solving Inventory (CPSI). Results: Among these students, 52% reported on the level of health risk stress (HRS), 55.3% had depression symptoms. There were significant correlation among depression symptoms, perceived stress and problem solving appraisal. The causal relationship between them were identified with the path analysis method, problem solving in special, seems to have both direct and indirect effect on the prediction of depression and on perceived stress. Conclusion:There is a high level of college student perceived stress and depression symptoms. Problem solving appraisal is an important factor for both of them.

Angiotensin converting enzyme 2 (ACE2) is a new member of renin-angiotensin system found recently. It counterbalances the effects of angiotensin converting enzyme (ACE), hydrolyzes angiotensin Ⅱ to generate angiotensin-(1-7) and hydrolyzes angiotensin Ⅰ to produce angiotensin-(1-9). Angiotensin-(1-9) can be hydrolyzed by ACE to form angiotensin-(1-7), and angiotensin-(1-7) counteracts the effects of angiotensin Ⅱ in many aspects. Bradykinin or atrial natriuretic factor or substance P are not the substrates of ACE2, and ACE2 hydrolyzes the metabolites of bradykinin such as des-Arg~9-BK and des-Arg~(10)-kallidin, and also the dynorphin A, one of the opium peptides. ACE2 is not inhibited by angiotensin-converting enzyme inhibitor (ACEI). Recent studies demonstrate that ACE2 is a functional receptor of SARS coronavirus. To elucidate the mechanisms of cardiovascular diseases and other disease such as SARS, further investigations in ACE2 are needed.