Objective:To investigate the expression and distribution of aquaporin 5(AQP5) in allergic rhinitis(AR)treated by fluticasone propionate and levocabastine.Method:Forty Wister rats were divided randomly into AR(n=30) and control groups(n=10). After AR models were established, the AR rats were divided evenly into F group, L group and AR control group.Three groups were treated respectively for 28 days, then the expression of AQP5 in nasal mucous membrane were detected by immumohistochemistry assay.Result:The distribution of AQP5 was consistent in all groups. The expression of AQP5 in F group was significantly different from L group and AR group (P < 0.05), while there was no significant difference between that of AR group and L group(P>0.05). The expression of AQP5 in L group was significantly different from that in control group(P<0.05).Conclusion:High expressions of AQP5 in rat with AR indicated the positive correlation between AQP5 and AR. AQP5 might be one of pathological factors of AR concered with gland's excessive secretion and tissue edema. Glucocorticoid can down-regulate the expression of AQP5,but H1-receptor antagonist can not reduce the expression of AQP5.

Objective: To study the influence of different telomere oligonucleotide on rat's thoracic aortic smooth muscle (A7R5 ) cell apoptosis and to clarify weather silent information regulator 1 (SIRT1)/cancer suppressor gene (P53) signaling pathway involved in oligonucleotide induced cell apoptosis. Methods: 3 telomere repeat sequences of TE-12 (TTAGGG)2, TE-24 (TTAGGG)4 and TE-36 (TTAGGG)6 were respectively transfected into rat's A7R5 cells as TE-12 group, TE-24 group and TE-36 group; in addition, blank A7R5 cells were used as Control group. Transfection induced A7R5 cell apoptosis was detected by flow cytometry, mRNA and protein expressions of SIRT1 and P53 in A7R5 cells were examined by RT-PCR and Western blot analysis in different groups. Results: Successful ransfection rates were similar among 3 groups. Compared with Control group and TE-36, TE-24 groups, the highest apoptosis rate of A7R5 cells was found in TE-12 group and the lowest was found in TE-24 group which was still higher than that in Control group, allP<0.05. mRNA and protein expressions of SIRT1 was obviously higher in TE-12 group than the other 3 groups, allP<0.05; mRNA and protein expressions of P53 was obviously lower in TE-24 group than the other 3 groups, allP<0.05. Conclusion: Telomere oligonucleotide sequence may promote rat's A7R5 cell apoptosis; different sequence had various influence and the strongest effect was observed in TE-12 sequence. The above impact might be related to SIRT1/P53 signaling pathway.

Objective To explore the composition situation of the risk factors of patients with stroke in Huainan area. Methods 471 continuous registration and hospitalized patients with stroke in Huainan were selected,of which,362 cases were ischemic stroke,109 cases were hemorrhagic stroke,and the known risk factors were statistically analyzed. Results Of all patients,the incidence of hypertension was first(69. 3% was in ischemic stroke, 77. 9% was in hemorrhagic stroke) ,and hypertension was the most important risk factors. The composition of risk factors in stroke patients of different gender were different,and the smoking,drinking problem was more prominent in male patients (51.1% ,33.2%respectively)than that in female patients(5.1% ,2.0% respectively) (x2 =111-81,67.62, all P＜0.01) ;The incidence of diabetes, heart disease, atrial fibrillation was higher in female patients (37. 6% ,31. 9% ,19.3%respectively) than that in male patients (22.3% ,20.4% ,8.8% respectively) (x2 = 13. 12,8.09,11. 12,all P＜0.01). Hypertension,diabetes accounted for only 40.8% and 47.4% of patients taking regular medication. Atrial fibrillation was more common in patients with hemorrhagic stroke. Conclusion Controlling blood pressure and blood sugar was still important in the primary prevention and secondary prevention of stroke in the region. People of different gender should be targeted for individual health education, and promoting a healthy lifestyle should be paid attention, so as to the occurrence of stroke could be effectively prevented.

Acupuncture Therapy ; Edema ; etiology ; immunology ; therapy ; Female ; Humans ; Lower Extremity ; pathology ; Middle Aged ; Ovarian Neoplasms ; surgery ; Postoperative Complications ; etiology ; therapy

Objective To understand the pollution status of indoor volatile organic compounds(VOCs) and to provide evidence for preventing the adverse effects of them on resident health.Methods According to the different times of the house decoration, 53 residents were randomly selected from a district of Dalian.Air was sampled in the bedrooms, the kitchens and the outdoors for 24 h by sampling tubes which were activated by activated carbon and the concentrations of VOCs were analyzed by GC-MS/ SIM.Results The concentrations of VOCs indoor were higher at the half year after decoration.The concentrations of methyl isobuthyl ketone, butyl acetate, 1, 2-dichloroethane, 1, 1, 1-trichlo-roethane, tetrachlorocarbon, hexane and buthanol were 0.60, 9.15, 3.10, 1.00, 1.67, 1.90 and 14.50 ?g/m3 in the bedrooms;they were 0.60, 8.10, 3.95, 1.74, 0.85, 1.87 and 12.45 ?g/m3 in the kitchens.The concentrations of VOCs indoor were still higher than those of outside atmosphere(P

Objective To investigate the possibility that TGF ? may also influence the basal and FSH stimulated production of estradiol and progesterone by granulosa cells in vitro. Methods Granulosa cells were obtained from infertile patients undergoing in vitro fertilization and embryo transfer treatment and cultured with serum free supplemented HAM′s F 10 medium(control) or increasing concentrations of TGF ? 1,2 in the absence or presence of FSH (96 U/ml). The media were collected in 24, 48, 72 and 96 h intervals and assayed for estradiol and progesterone. Results Increasing amounts of TGF ? had a significant effect on estradiol production, especially in the presence of FSH,The estradiol value was evaluated from (12 077.9?5 835.3) pmol/L and (13 656.1? 8 404 3) pmol/L to (16 606.8?7 993.3) pmol/L and (19 480.4?913.8) pmol/L. TGF ? inhibited FSH stimulated progesterone but not unstimulated progesterone release ( P

[Objective]To appraise the clinical effect of acupoint application on cough-asthma.[Method] Choose clinic 144 cases,measure FEV1,PEF and FEV1% after treatment,make statistical analysis,compare the difference of the appraisal indexes between before and after treatment,study the relation among age,sex and TCM syndromes.[Conclusion] After treatment,the symptoms score and lung function were markedly improved.[Conclusion] After treated with Dog-days Paste,the patients’ clinical symptoms were obviously relieved,and the lung functional indexes improved too.

Objective We aimed to study the optimal method for local compression following removal of needle after intravenous injection and protect patients' vein to the highest degree. Methods 620 patients were divided into the observation group and the control group with 310 cases in each group randomly. Comparison study was carried out by adopting interchange compression (routine method) and noninterchange compression (direct pressing with hypothenar). Results The incidence of pain, bleeding and subcutaneous stasis after removal of needle in the observation group was lower than those in the control group (P<0.01). Conclusions Local compression following removal of needle with hypothenar was superior to routine compression method and it was worth applying in clinics widely.

Dendritic cells are now given more attention th ese years in tumor immuno-therapy. In this review, we summarized the advances o f dendritic cells vaccines and their applications in malignant tumors such as me lanoma and renal carcinoma.

Objective To observe the therapeutic effect of Ningxin Granule Heart-Quieting Granule for pediatric viral myocarditis.Methods Totally 60 cases of pediatric viral myocarditis were randomly divided into treatment group (n=30) and control group (n=30).There was no significant difference between two groups in sexuality,age and pathogenetic condition.Patients in treatment group took Ningxin Granule,while ATP,CoA,Vit C,Inosine and Ribavirin were given to control group by intravenous drip.Results The treatment group was superior to control group in relieving symptoms,reducing the bearing premature,CK-MB,cTnI and LVEDD,and increasing LVEF.Conclusion Ningxin Granule can obviously relieve symptoms and signs,reduce the bearing premature,decrease the level of myocardial enzyme and improve the cardiac function.