Objective To evaluate the performance verification of serum copper detected by Roche Cobas 8000 automatic biochemical analyzer.Methods Referring to CLSI files and other documents,the precision,accuracy,analysis of measuring range and reference interval of serum copper from Roche Cobas 8000 automatic biochemical analyzer were verified.Results The coefficient of variations (CV) of within-run precision with high and low values were 2.96％,2.91％,respectively;CV of the between-run precision with high and low values were 3.07％,2.99％,respectively,which were all less than the CV provided by the manufacturer (10.00％).The bias between detection results and target value were 1.78％ and 1.56％,respectively,which were all within the measurement range of quality assessment.Analysis of measuring range of the detected items was in line with the linear range provided by the specification.The quotative biological reference range conformed to the group of this laboratory services.ConclusionThe main analytic characteristic of serum copper measurement by Roche Cobas 8000 automatic biochemical analyzer is consistent with acceptable quality standards,and the experimental evaluation project of CLSI shows maneuverability and practicability.

Antigens, CD1 ; metabolism ; Child, Preschool ; Cholangitis, Sclerosing ; diagnosis ; etiology ; metabolism ; Diagnosis, Differential ; Female ; Histiocytosis, Langerhans-Cell ; complications ; metabolism ; pathology ; Humans ; Immunohistochemistry ; Liver ; metabolism ; pathology ; S100 Proteins ; metabolism

Animals ; Apoptosis ; Cardiomegaly ; metabolism ; pathology ; Glycogen Synthase Kinase 3 ; metabolism ; Mice ; Mice, Transgenic ; Myocytes, Cardiac ; cytology

Objective To test the effect of hypothermia on Na+/H+ exchange, activated by shrinkage and cytoplasmic acidosis. Method Amiloride-sensitive Na+ influx in guinea pig red cells was traced with isotope 22Na and intracellular Na+ concentration was measured by emission flame photometry. Result Amiloride-sensitive Na+ influx decreased linearly as a function of temperatures (about 37℃) in shrunken cells, but increased in acidified cells. The up-regulation of acid-induced Na+／H+ exchange by elevated temperature was enhanced by hypo-osmolarity. Less sensitivity of intracellular H+ site at 41℃ may be the mechanism for the inhibition of shrinkage-induced Na+/H+ exchange by elevated temperature. Heating-mediated explosive increase in the activity of acid-induced Na+/H+ exchange may be due to enhanced extracellular Na+ sensitivity and lower intracellular pH caused by acidic metabolites. Acid-induced Na+/H+ ewxchange contributes to cytoplasmic Na+ accumulation. Conclusion These two modes of Na+/H+ exchange with different response to elevated temperature may play different roles in the cellular pathogenesis of heatstroke.

Objective To investigate the principles of combined traditional Chinese and western medicine treatment of early postoperation inflammatory small bowel obstruction(EPISBO).Methods 93 cases of EPISBO were randomly divided into two groups including group A,47 cases received combined traditional Chinese and west- ern medicine treatment,and group B 46 received only western medicine treatment.Results 93 patients were all cured through nonsurgical treatment.The combined traditional Chinese and western medicine treatment was better in symtom improvement and reduce of expense.Conclusion The principle treatment of EPISBO is mainly nonsurgical. The traditional Chinese medicine and somatostatin are very important.

Objective To measure the levels of serum transferrin receptors (sTfR) and serum ferritin (SF) in children with hemoglobin H disease for investigation the iron metabolism(sideropenic or iron overload) and to guide the proper intervention.To get a best index of judging the iron overload in HbH children by analysis of the values of sTfR and sTfR/lgSF.Methods Peripheral blood specimens were obtained from 50 cases of HbH children and 30 cases of healthy normal controls,the levels of sTfR and SF were examined by enzyme linked immunosorbent assays(ELISA).The diagnosis efficiency of sTfR and sTfR/lgSF in HbH children′s iron metabolism was judged by receiver operator cha-racteristic (ROC) curve analysis.Results The level of sTfR in HbH group was (6.74?1.02) mg?L-1,which was significantly lower than that in healthy control group[(8.09?0.67) mg?L-1](P

Objective To observe the effect of transforming growth factor β1 (TGF-β1) on thyroid peroxidase activity and the expression of mRNA in primary porcine thyrocytes, and approach its possible mechanism. Methods In vitro cultured porcine thyrocytes were divided into 0 μg/L(control group), 2, 5, and 10 μg TGF-β1/L groups.After cultured for 72 h, the cell survival rate was measured with methyl thiazolyl tetrazolium (MTT) assay and the thyroid peroxidase (TPO) activity was measured by guaiacol method. The expression of TPO mRNA was determined cell survival rates of 2, 5, 10 μg/L groups(85.26%, 75.14% and 63.21%) were significantly lower(all P < 0.05 ).significantly decreased as compared with the control group [(2.143 ± 0.102), all P < 0.05)], and the TPO of TGF-β1, the lower the expression of TPO mRNA [(0.875 ± 0.078), (0.466 ± 0.044), (0.273 ± 0.007)] compared with the control group[(1.419 ± 0.148), all P < 0.05)]. Conclusions The inhibiting effect of TGF-β1 on primary cultured porcine thyrocytes may be related to its mechanism of inhibiting TPO activity and reduced mRNA expression.

Objective To understand the status and distribution of the Xinjiang Uygur and Han middle-aged and elderly population who suffer from cardio-cerebral vascular disease.Methods From July 2004 to June 2007,multi-stage stratified random cluster sampling methods was adopted to collect the random samples older than 50-year-old suffering from cardiovascular and cerebrovascular disease in South Xinjiang.Results In the 3685 Uygur and Han ethnic elderly people,the prevalence of hypertension,diabetes,lipid disorders,cerebrovascular disease and dementia were 28.20%,6.70%,15.39%,12.46% and 4.10% respectively in the southern border regions of Xinjiang.Except hypertension,the prevalence rate of other cardiovascular and cerebrovascular diseases in Uygurs and hans had significant differences.The survey found that the prevalence rate of dyslipidemia and diabetes in Hans was higher than the Uygurs,while the Uygurs prevalence rate of cerebrovascular disease and dementia.Conclusion The Xinjiang Uygur and Han elderly are faced with a grim situation of cardiovascular and cerebrovascular diseases;there are significant differences for the nationality and gender.

To study the effects of exercise and weight control on bone mineral density (BMD) of girl athletes, dual X-ray and single X-ray absorptiometry were adopted to measure BMD; And serum Vitamin D3, estrogen, cholesterol and alkaline phosphatase levels of subjects were determined. Subjects were 63 students of two age groups (before puberty. 8-9 years old; after puberty: 15-16 years old, respectively) of sports and ordinary schools. The results showed that.. Exercise training is beneficial to increase BMD; Long term weight control had no effect on BMDof athletes in this study; Yet girl athletes after puberty with low estrogen levels had less BMD; Girl judo players with rapid weight loss had higher BMD as compared with the same aged nontraining students.

ObjectiveTo probe the short-term efficacy of endovascu lar stent-as sisted angioplasty for basilar artery stenosis.MethodsTwenty p atients with s ymptomatic basilar artery stenosis were treated by angioplasty using a balloon-e xpandable coronary stent.ResultsThe basilar artery caliber r eturned to norma l size in 12 cases and restored by more than 80% in the remaining 8 cases. No tr ansient ischemic attack (TIA), stroke, or other peri-operative complications rec urred. Follow-up angiography in 13 patients revealed no re-stenosis.Co nclusionsShort-term efficacy of endovascular stenting for basilar arte ry stenosis is promising.