Objective To evaluate the performance verification of serum copper detected by Roche Cobas 8000 automatic biochemical analyzer.Methods Referring to CLSI files and other documents,the precision,accuracy,analysis of measuring range and reference interval of serum copper from Roche Cobas 8000 automatic biochemical analyzer were verified.Results The coefficient of variations (CV) of within-run precision with high and low values were 2.96％,2.91％,respectively;CV of the between-run precision with high and low values were 3.07％,2.99％,respectively,which were all less than the CV provided by the manufacturer (10.00％).The bias between detection results and target value were 1.78％ and 1.56％,respectively,which were all within the measurement range of quality assessment.Analysis of measuring range of the detected items was in line with the linear range provided by the specification.The quotative biological reference range conformed to the group of this laboratory services.ConclusionThe main analytic characteristic of serum copper measurement by Roche Cobas 8000 automatic biochemical analyzer is consistent with acceptable quality standards,and the experimental evaluation project of CLSI shows maneuverability and practicability.

Animals ; Apoptosis ; Cardiomegaly ; metabolism ; pathology ; Glycogen Synthase Kinase 3 ; metabolism ; Mice ; Mice, Transgenic ; Myocytes, Cardiac ; cytology

Antigens, CD1 ; metabolism ; Child, Preschool ; Cholangitis, Sclerosing ; diagnosis ; etiology ; metabolism ; Diagnosis, Differential ; Female ; Histiocytosis, Langerhans-Cell ; complications ; metabolism ; pathology ; Humans ; Immunohistochemistry ; Liver ; metabolism ; pathology ; S100 Proteins ; metabolism

Objective To test the effect of hypothermia on Na+/H+ exchange, activated by shrinkage and cytoplasmic acidosis. Method Amiloride-sensitive Na+ influx in guinea pig red cells was traced with isotope 22Na and intracellular Na+ concentration was measured by emission flame photometry. Result Amiloride-sensitive Na+ influx decreased linearly as a function of temperatures (about 37℃) in shrunken cells, but increased in acidified cells. The up-regulation of acid-induced Na+／H+ exchange by elevated temperature was enhanced by hypo-osmolarity. Less sensitivity of intracellular H+ site at 41℃ may be the mechanism for the inhibition of shrinkage-induced Na+/H+ exchange by elevated temperature. Heating-mediated explosive increase in the activity of acid-induced Na+/H+ exchange may be due to enhanced extracellular Na+ sensitivity and lower intracellular pH caused by acidic metabolites. Acid-induced Na+/H+ ewxchange contributes to cytoplasmic Na+ accumulation. Conclusion These two modes of Na+/H+ exchange with different response to elevated temperature may play different roles in the cellular pathogenesis of heatstroke.

Objective:To observe the clinical effect of endodontic therapy and full crown restoration in the treatment of cracked teeth. Methods:The trace and depth of 50 cracked teeth were carefully examined under root canal microscope.According to the depth and scope of the cracks,the cracked teeth were classficated into the degree of Ⅰ,Ⅱ and Ⅲ,treated endodontically and restored with full crown.Results:During 1 year follow-up,34 cases showed successful effect,5 progressive,1 1 failure.The general effectiveness rate was 78.0%.The effectiveness rate of group Ⅰ,Ⅱ and Ⅲ was 93.3%,82.1 % and 28.6% respectively.Group Ⅰand Ⅱ vs Ⅲ,P <0.05,Ⅰvs Ⅱ,P >0.05.Conclusion:The treatment effect of cracked teeth is closely related to the depth and scope of cracks.

A miniaturized atomic emission spectrometer composed of a corona discharge source and a handheld charge-coupled device detector was constructed for the sensitive determination of trace mercury, with photochemical vapor generation for sample introduction. By using formic acid, mercury vapor was generated under the irradiation of UV light, and then the vapor was introduced into the corona discharge area with the carrier gas flow of argon. The optical signals were captured and analyzed by the charge-coupled device detector at 253. 7 nm. Under the optimized conditions including 4% formic acid, discharge voltage of 90 V, and carrier gas flow rate of 200 mL/min, the stable detection signals were obtained ( RSD=2. 5%). The calibration curves were plotted with the concentration of mercury ranging from 0. 5 to 1000 ng/mL, with a correlation coefficient >0. 99 and the limit of detection of 0. 03 ng/mL. Two certified reference materials were analyzed, and a t-test demonstrated that the determined results by the proposed method had no significant difference with the certified values at the confidence level of 95%. Water samples collected from both Funan River in Chengdu and the Lotus Pond on campus of Sichuan University were analyzed as well, with the recoveries of standard addition between 93 . 8% and 103%. The miniaturized instrumentation demonstrated several advantages including easy construction, low power consumption, high sensitivity, and portability for field analysis.

Objective To study the influence of laparoscopic gastric surgery on the gut barrier function (GBF).Methods There were 64 gastric cancer patients undergoing respectively laparoscopic radical procedures (32 cases) and open gastric surgery (32 cases).Blood was drawn on day one before surgery,day 1,day 3 and day 7 after the surgery for the measurement of plasma D-lacate and plasma diamine oxidase activity by using ultraviolet spectrophotometry.Results There was no statistical difference among demographic,clinicopathological characterastics between the two groups (P ＞ 0.05).The difference on the operative time,blood loss and the time starting to take food after the surgery was significant between the two groups,P ＜ 0.05.The differences of plasma D-lacate level and the diamine oxidase (DAO) on perioperatively respective all time points were not significant between the two groups,P ＞ 0.05.Conclusion Laparoscopic radical gastrectomy is comparable to open procedures in causing damage to patient's gut barrier functions.

Objective To study the effect and safety on the retention respiratory inhalation general anesthesia in craniocerebral operation.Methods Eighty-six patients undergoing craniocerebral operation were divided into conventional anesthesia group (group A) and retention respiratory anesthesia group (group B) by random digits table method with 43 cases each.Group A was given conventional induction and intubation,administration order:midazolam 0.05 mg/kg,fentanyl 2-4 μ g/kg,cisatracurium 0.2-0.3 mg/kg,propofol 1.0-1.5 mg/kg;group B was given rapid induction and intubation,administration order:midazolam 0.05 mg/kg,fentanyl 2-4 μg/kg,propofol 1.0-1.5 mg/kg,succinylcholine 1.5 mg/kg.The 2 groups after intubation done by Ohmeda 7100 anesthesia machine control respiration,trace pump continuous infusion of propofol and remifentanil,and maintained inhaling isoflurane anesthesia.Group A was discontinuity intravenous injection atracurium.Group B was changed autonomous breathing after the succinylcholine effect disappeared,respiratory frequency according to the regulation of remifentanil infusion rate,the respiration rate remained at 8-12 times/min.The consumption of propofol and remifentanil,additional times of fentanyl,intraoperative airway pressure,partial pressure of carbon dioxide in end expiratory gas (PE TCO2),intraoperative body movement,airway pressure,extubation time,OAA/S score after extubation 15 min and untoward reaction were recorded.Results There were no statistical differences in operation time,intraoperative body movement,intraoperative airway pressure and PETCO2 between the 2 groups (P＞ 0.05).The consumption of propofol and remifentanil,additional times of fentanyl in group B were significantly higher than those in group A [(69.2 ± 13.7) μg/(kg·min) vs.(61.0 ± 8.2)μg/(kg·min),(0.19 ± 0.06) μ g/ (kg· min) vs.(0.15 ± 0.05) μ g/ (kg· min),1.5 times vs.0 time],there were statistical differences (P＜ 0.05).The extubation time in group B was significantly shorter than that in group A [(6.4 ±3.2) min vs.(11.3 ± 4.5) min],the OAA/S score after extubation 15 min in group B was significantly lower than that in group A [1 (1-3) scores vs.2(1-3) scores],there were statistical differences between the 2 groups (P ＜ 0.05).Conclusion Retention respiration intubation general anesthesia patients of craniocerebral operation can early recover,extubation time is significantly shorter,it is a safe and effective anesthesia method.

Objective To understand the status and distribution of the Xinjiang Uygur and Han middle-aged and elderly population who suffer from cardio-cerebral vascular disease.Methods From July 2004 to June 2007,multi-stage stratified random cluster sampling methods was adopted to collect the random samples older than 50-year-old suffering from cardiovascular and cerebrovascular disease in South Xinjiang.Results In the 3685 Uygur and Han ethnic elderly people,the prevalence of hypertension,diabetes,lipid disorders,cerebrovascular disease and dementia were 28.20%,6.70%,15.39%,12.46% and 4.10% respectively in the southern border regions of Xinjiang.Except hypertension,the prevalence rate of other cardiovascular and cerebrovascular diseases in Uygurs and hans had significant differences.The survey found that the prevalence rate of dyslipidemia and diabetes in Hans was higher than the Uygurs,while the Uygurs prevalence rate of cerebrovascular disease and dementia.Conclusion The Xinjiang Uygur and Han elderly are faced with a grim situation of cardiovascular and cerebrovascular diseases;there are significant differences for the nationality and gender.

To study the effects of exercise and weight control on bone mineral density (BMD) of girl athletes, dual X-ray and single X-ray absorptiometry were adopted to measure BMD; And serum Vitamin D3, estrogen, cholesterol and alkaline phosphatase levels of subjects were determined. Subjects were 63 students of two age groups (before puberty. 8-9 years old; after puberty: 15-16 years old, respectively) of sports and ordinary schools. The results showed that.. Exercise training is beneficial to increase BMD; Long term weight control had no effect on BMDof athletes in this study; Yet girl athletes after puberty with low estrogen levels had less BMD; Girl judo players with rapid weight loss had higher BMD as compared with the same aged nontraining students.