Objective To assess the therapeutic effect and safety of Chinese medicine with the actions of activating blood and removing stasis in the treatment of acute coronary syndrome(ACS)patients.Methods Sixty-six patients with ACS were randomly assigned to the control group,the Danshen Injection(DI) group and the DI combined with Chuanxiongzine Injection(CI) group.All of the three groups received antithrombotic therapy besides the corresponding medicine according to the experimental design.The therapeutic effect and safety of the three groups were observed and compared after treatment.Results The hemorheology of ACS patients in the three groups was improved(P

Objective To investigate the protective effects of quercetin on primary cultured SD neonate rats cardiomyocyte injury induced by daunorubicin.Methods Cultured cardiomyocytes were divided into blank groups,DNR groups,DNR+QUE groups,QUE groups.After preincubation of cardiomyocytes for 24 hours,cytoprotection effect was assessed by cell morphous,and cell apoptotic rates determined by flow cytometry,extracellular lactate dehydrogenase(LDH),superoxide dismutase(SOD) and malondialdehyde(MDA).Results Compared with DNR groups,the shedded cardiomyocytes and the cell debris of DNR+QUE groups decreased;the cardiomyocyte apoptosis rates decreased;the content of LDH and MDA decreased(P

Background In vitro study showed that chemotaxis consist of chemotaxis factor 4(CXCR4)and stromal cells derived factor-1(SDF-1)and may play a role in the orientation and migration of retinal progenitor cells (RPCs)toward lesion.Overexpression of CXCR4 in RPCs can enhance the chemotaxis activity.Objective This work was to explore the feasibility and underlying mechanism of up-regulation of CXCR4 on RPCs induced by hypoxia.Methods RPCs were retained in an incubator with normal O2volume(16％)or hypoxia condition(10％ O2)for 12 hours and 24 hours respectively.Flow cytometer cell analysis screening(FACS)was conduced to measure the proportion of CXCR4-expressing cells,and CXCR4,HIF-1 mRNA were analyzed by reverse transcription-polymerse chain reaction(RT-PCR).The chemotical effect of 30 mg/L SDF-1 to RPCs cultured under the hypoxia condition was assessed using Boyden chamber.Results The expression level of CXCR4(CXCR4 mRNA/β-actin mRNA)inRPCs cultured by 10％ O2 for 12 and 24 hours were 0.28+0.07and 0.48+0.17 and increased by 1.75 and 3.00 fold more than that of 16％ O2 culture group(0.16+0.02)(P＜0.01).The expression level of HIF-1 mRNA(HIF-1 mRNA/β-actin mRNA)in RPCs cultured by 10％ O2 for 12 and 24 hours were 0.18 ±0.07and 0.38 ±0.13 and increased by 3.00 and 6.30 fold more than that of 16％ O2 culture group(0.06±0.01)(P＜0.01).The chemotical effect of 30 μg/L SDF-1 to RPCs increased from 13.00％ in 16％ O2 culture group to 36.00％ and 46.00％ in the cells cultured by 10％ O2for 12 and 24 hours.FACS revealed that the proportion of CXCR4+ cells in hypoxia-exposure for 12 and 24 hours were 26.90％ and 46.10％,respectively,but that in 16％ O2 culture group was 9.10％,showing a statistically significant difference(P ＜ 0.01).Conclusions RPCs induced by hypoxia can enhance the expression of CXCR4 in RPE cells and the chemotaxia to SDF-1.The overexpression of H1F-1 in RPCs may be involved in the up-regulation of CXCR4 expression.

Adrenergic beta-Antagonists ; Arrhythmias, Cardiac ; Humans

Animals ; Electrophoresis, Gel, Two-Dimensional ; methods ; Gene Expression Profiling ; Humans ; Neoplasm Proteins ; analysis ; isolation & purification ; Neoplasms ; chemistry ; Proteome ; analysis ; isolation & purification ; Proteomics ; methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; methods

Administration, Inhalation ; Animals ; Erythrocyte Count ; Erythrocytes ; drug effects ; Female ; Formaldehyde ; toxicity ; Hemoglobins ; analysis ; Male ; Mice

Objective To study the influence and mechanism of seawater immersion on endothelial cell injury sustained by burn firearm combined injury to improve the early therapeutic efficacy. Methods The dogs with burn firearm combined injury were randomly divided into two groups: immersion group and control group. In immersion group, the dogs were immersed in seawater for 4 hours, then taken out from seawater. Blood samples were collected from central vein at 4 h, 7 h, 10 h, 20 h and 28 h following wound for the detection of changes of the circulating endothelial cells (CEC) and von Willebrand Factor (vWF). The same procedures except immersion were performed in the control group. Results The levels of CEC and vWF elevated at 4 h and 7 h following wound in control group( P

Objective:To investigate serum level of vascular endothelial growth factor(VEGF)in patients with rheumatoid arthritis(RA).Methods:VEGF ELISA Quantikine kit.The serum RF level was also d etermined using Beckman Array 360.Results:The serum concen tration of VEGF was significantly higher in patients with RA than in healthy co ntrol(P＜0.01).Conclusion:It suggests that VEGF is involved in the pathog enesis of RA and that measurment of serum concentration of VEGF is noninvasive, usful method for monitoring the disease activity of RA.

Objective To study the influence of seawater immersion on hemostatic function after burn in dogs. Methods Twenty healthy adult mongrel dogs of beth sexes weighing 12-15 kg were randomly divided into two groups: immersion group(n=10) and control group(n=10). The animals were anesthestized with 3% pentobarbital 30 mg?kg~(-1) i.v., intubated and mechanically ventilated. 5F Swan-Ganz catheter was placed via right internal jugular vein. 10% of the skin on the back was burnt(second degree). In immersion group the animals were immersed in seawater containing salt 25.3 g?L~(-1)(pH8.1, T21-23℃) with head and neck kept above water for 4h. In control group the animals suffered second degree burn of same area without being immersed in seawater. Blood samples were taken from Swan-Ganz catheter before burn(baseline)and 4,7,10,20,28 h after burn for determination of(1) circulating endothelial cell(CEC) count, (2)tissue-type plasminogen activator(t-PA), (3) plasminogen activator inhibitor(PAI) and (4) thromboxane B_2/6-keto-prostaglandin F_(1?)(TXB_2/6-k-PGF_(1?)). At the end of the experiment lung tissue was obtained for microscopic examination. Results The blood CEC count, PAI-1 level and TXB_2/6-k-PGF_(1?) ratio significantly increased while t-PA level significantly decreased at 4 h after burn in control group but at 4-28 h after burn in immersion group. The differences between the two groups were significant. Microscopic examination of the lung showed some thrombi in immersion group. Conclusion Burn causes acute damage to the endothelial cells of the whole body and disorder of hemostasis. They are more severe and last longer after seawater immersion.