BACKGROUND: Peripheral blood stem cell transplantation in children has some difficulties compared with adult cases. Despite the children's total blood volume is small, they have a relatively higher volume of blood per weight than adults. Also, it is hard for the children to maintain 2 central vein during leukapheresis to shorten the time consumed. We compared the artery-harvest and central vein-harvested group for possible risk and stem cell yield in pediatric patients with malignancies. METHODS: From August, 1995 to January, 1997, 21 leukapheresis was performed by 7 patients. The patients who could have 2 central veins for leukapheresis were included in vein-harvested group and the patients who could have only 1 central vein were included in artery-harvested group. The peripheral vein was not used for leukapheresis. COBE Spectra(COBEBCT, Lakewood, CO) was used and priming was done by packed RBCs in patients weighing less than 20kg. Stem cell yield was assessed by MNC, CD34+, CFU-GM, respectively. During leukapheresis, the patients were closely monitored for change in vital sign, evidence for thrombosis, bleeding, hypocalcemia, etc. RESULTS: There was no serious complication in each group of patients. After the leukapheresis, WBC and platelet count decreased but Hb level was increased due to reinfusion of primed packed RBCs. Average flow rate was higher in vein-harvested group but there was no difference in time consumed and results in stem cell assay. CONCLUSION: Peripheral stem cell harvest in children by radial artery can be performed safely and easily without an increased risk or complication. In younger children, it is possible to achieve even more higher stem cell yield. If the patient is unable to maintain 2 central vein for leukapheresis, transient radial arterial catheterization is a safe and convenient method.
Adult ; Blood Volume ; Catheterization* ; Catheters* ; Child ; Colony-Forming Units Assay ; Granulocyte-Macrophage Progenitor Cells ; Hemorrhage ; Humans ; Hypocalcemia ; Leukapheresis ; Peripheral Blood Stem Cell Transplantation ; Platelet Count ; Radial Artery ; Stem Cells* ; Thrombosis ; Veins ; Vital Signs

We report a case of infantile digital fibromatosis in a 34 month-old boy, who presented with a painless subcutaneous tumor on the medial aspect of the left third toe. A histological examination showed scattered small, round eosinophilic inclusion bodies in the cytoplasm of the tumor cells, which was consistent with infantile digital fibromatosis. A'immunohistochemical study revealed that desmin, a-smooth muscle actin, and vimentin were clearly positive in the cytoplasm of the tumor cells, but the inclusions themselves showed negative staining, thus indicating a hollow-like staining pattern. Electron microscopy showed either well-defined or ill-demarcated dense bodies in the cytoplasm of the tumor cells. In some areas, small vesicles and intracellular organells were observed' in the inclusions. In the course of conservative treatment, a new lesion developed on the lateral aspect of the left third toe, seven months after the appearance of the initial lesion.
Actins ; Cytoplasm ; Desmin ; Eosinophils ; Fibroma* ; Humans ; Inclusion Bodies ; Male ; Microscopy, Electron ; Negative Staining ; Toes ; Vimentin

BACKGROUND: Evening primrose oil(EPO) is a rich source of cis-linoleic acid and gammalinolenic acid(GLA) and has been used as a therapeutic agent in various skin diseases such as atopic dermatitis. OBJECTIVE: The purpose of this study was to evaluate the suppressive effect of EPO on murine contact sensitivity. METHODS: BALB/c mice were divided into 3 groups, positive control, experimental and negative control groups: the positive control group represents a group of mice which were sensitized and challenged with DNFB, the experimental group represents EPO-pretreated positive control group and the negative control group represents a group of mice which were challenged only. The changes of ear thickness were measured, and H & E staining and immunohistochemical staining for ICAM-1 expression of ear skin were performed to evaluate the histological changes of each group. RESULTS: The Pretreatment of mice with EPO resulted in suppression of contact sensitivity by more than 82%. On H & E staining, only a mild inflammatory reaction was observed in the dermis. Also ICAM-1 expression of keratinocytes, the intensity of the staining was significantly decreased in the experimental group compared with positive group. CONCLUSIONS: Our study indicates that EPO was able to suppress the induction of contact sensitivity.
Animals ; Dermatitis, Atopic ; Dermatitis, Contact* ; Dermis ; Dinitrofluorobenzene ; Ear ; Intercellular Adhesion Molecule-1 ; Keratinocytes ; Mice ; Oenothera biennis* ; Skin ; Skin Diseases

BACKGROUND: Data on the frequency of Herpes Zoster are sparse, especially in inpatients OBJECTIVE: The purpose of this study was to elucidate the epidemilogy and the clinical characteristics of herpes zoster in inpatients. METHODS: During a 3-year-Period from Jan. 1991 to Dec. 1991, 157patients with herpes zoster were evaluated in regard to annual, monthly and seasonal incidence age incidenee and sex ratio. Patients with herpes zoster were further assessed concerning associratel conditions, dermatomic distributions and complications. RESULTS: 1. The annual rate ranged from 2.6% to 3.3% (mean 2.8%) and the ratio of male to female was 0.85: 1. 2. There were no marked manthly of spasonal differences in herpsoster, ranging from 4.5% to 10.8% and 21.7% to 31.2%, respectively. 3. Herpes Zoster was seen most frequently in the 50-59 year age group(27.4%) 4. The dermatomic involvernent of herpes zoster was most freqer t of thoracic dermatome(51.6%) and others were trigeminal(19.1%), cervical(13.4%), lumbar(6.4%), sacral(5.7%), facial(1.9%) and generalized(1.9%). 5. The frequency of associated conditions in herpes zoster was in d scending order, hypertension, tuberculosis, diabetes mellitus, malignancy, SLE, gastric ulcer, chronic renal failiure, rheumatoid arthritis, idiopathic pulmonary fibrosis. 6. The most common complication of herpes zoster was postheret c neuralgia and others were ophathalmologic complications, secondary bacterial infection, keloicl, formation, generalized varicelliform eruption, recurrent herpes zoster. CONCLUSION: Herpes Zoster is a major problem in the Department of Dermatology, especially in inpatients.
Arthritis, Rheumatoid ; Bacterial Infections ; Dermatology ; Diabetes Mellitus ; Female ; Herpes Zoster* ; Humans ; Hypertension ; Idiopathic Pulmonary Fibrosis ; Incidence ; Inpatients* ; Male ; Neuralgia ; Seasons ; Sex Ratio ; Stomach Ulcer ; Tuberculosis

BACKGROUND: Data on the frequency of Herpes Zoster are sparse, especially in inpatients OBJECTIVE: The purpose of this study was to elucidate the epidemilogy and the clinical characteristics of herpes zoster in inpatients. METHODS: During a 3-year-Period from Jan. 1991 to Dec. 1991, 157patients with herpes zoster were evaluated in regard to annual, monthly and seasonal incidence age incidenee and sex ratio. Patients with herpes zoster were further assessed concerning associratel conditions, dermatomic distributions and complications. RESULTS: 1. The annual rate ranged from 2.6% to 3.3% (mean 2.8%) and the ratio of male to female was 0.85: 1. 2. There were no marked manthly of spasonal differences in herpsoster, ranging from 4.5% to 10.8% and 21.7% to 31.2%, respectively. 3. Herpes Zoster was seen most frequently in the 50-59 year age group(27.4%) 4. The dermatomic involvernent of herpes zoster was most freqer t of thoracic dermatome(51.6%) and others were trigeminal(19.1%), cervical(13.4%), lumbar(6.4%), sacral(5.7%), facial(1.9%) and generalized(1.9%). 5. The frequency of associated conditions in herpes zoster was in d scending order, hypertension, tuberculosis, diabetes mellitus, malignancy, SLE, gastric ulcer, chronic renal failiure, rheumatoid arthritis, idiopathic pulmonary fibrosis. 6. The most common complication of herpes zoster was postheret c neuralgia and others were ophathalmologic complications, secondary bacterial infection, keloicl, formation, generalized varicelliform eruption, recurrent herpes zoster. CONCLUSION: Herpes Zoster is a major problem in the Department of Dermatology, especially in inpatients.
Arthritis, Rheumatoid ; Bacterial Infections ; Dermatology ; Diabetes Mellitus ; Female ; Herpes Zoster* ; Humans ; Hypertension ; Idiopathic Pulmonary Fibrosis ; Incidence ; Inpatients* ; Male ; Neuralgia ; Seasons ; Sex Ratio ; Stomach Ulcer ; Tuberculosis

We have experienced two cases of extramammary Paget's disease. The first case was a 68-year-old male who showed erythematous, crusted, oozing, eczematoid patches on the scroturn, penile root and pubic area, and verrucous plaque on the left pubic area of 5 years' duration. The second case was a 60-year-old male who showed erythematous, oozing, hyperkeratotic plaque and nodular mass on the scrotum of 4 years' duration. In electron microscopic findings of the first case, the Paget's cells was suggested to be formed by pleuripotential germinative cells; well developed microvilli, numerous electron lucent secretory granules, a few electron dense granules, apocrine type secretion and well developed desmosome.
Aged ; Desmosomes ; Humans ; Male ; Microvilli ; Middle Aged ; Paget Disease, Extramammary ; Scrotum ; Secretory Vesicles

No abstract available.
Heel* ; Molluscum Contagiosum* ; Weight-Bearing* ; Zea mays

Authors reviewed 264 cases of meconium aspiration pneumonia of the newborn at Sohwa Children's Hospital from July 1981 to June 1984. The radiologic findings were retrospectively analysed with particular attention to the degree of pulmonic infiltrations. The results were as follow: 1. The male to female ratio was 1.8:1 and clinical conditions which commonly associated were postterm infants (41.3%), placental dysfunction syndrome (33%) and perinatal asphyxia (11.4%). 2. There were improved (76.9%) and expired cases (12.1 %) as clinical course. 3. Radiologic findings were noticed .as pulmonic infiltration (68.2%), hyperinflation (35.2%), pneumomodiastinum (18.6%), pleural effusion (14.4%), pneumothorax (13.3%) and suggestive interstitial emphysema (6.8%). The more the degree of pulmonic infi ltration was been severe, the more the incidence of other pulmonic lesions was increased. 4. Pulmonic infiltrations were commonly occured in both lungs but pneumothorax, pleural effusion and hyperinflation in right. 5. Radiologic findings of 32 expired cases were noticed as pulmonic infiltrations (93.8%), hyperinflation (53.1 %), pneumomediastinum (37.5%), pneumothorax (18.8%), suggestive interstitial emphysema (18.8%) and pleural effusion (18.8%).
Asphyxia ; Emphysema ; Female ; Humans ; Incidence ; Infant ; Infant, Newborn ; Infant, Newborn ; Lung ; Male ; Meconium Aspiration Syndrome ; Meconium ; Mediastinal Emphysema ; Pleural Effusion ; Pneumonia ; Pneumothorax ; Retrospective Studies

No abstract available.
Diagnosis* ; Middle Lobe Syndrome* ; Thorax*

Leukemia cutis are clinically divided into the specific and nonspecific types. The former demon strates a malignant cell infiltrate on histopathologic examination. We report a case of leukemia cutis in a 29-year old female which developed specific cutanous manifestations previous to peripheral blood abnormalities, She had suffered from several discrete, bean to walnut sized, tender, erythematous patches and nodules on the trunk for 3 months and these gradually spread to the right cheek and upper arm. Atypical lymphocytes and incesassed lymphoblasts were notifced on a peripheral blood smear and bone marrow aspiration. A bone marrow biopsy revealed compact marrow finding, and increased celluarity. The histopathlogic findings showed diffuse dermal and subcutaneous infiltration of atypical lymphocytes. An immunohistochemical study with kappa, lamda, and leukocyte common antigen(LCA) revealed lymphoid malignancy, T-cell type. We treated her with various combined chemotherapy for 2 year, but her skin lesions were not inproved.
Adult ; Arm ; Biopsy ; Bone Marrow ; Cheek ; Drug Therapy ; Female ; Humans ; Juglans ; Leukemia* ; Leukocytes ; Lymphocytes ; Skin ; T-Lymphocytes