Objective To investigate the effect of nicardipine on the time-response relationships of vecuronium and atracuriumMethods Forty ASA grade Ⅰ-Ⅱ patients undergoing elective surgical operations were randomly allocated to four groups A single dose of vecuronium was intravenously administered at twice ED 95(01mg?kg -1) in groupⅠand combined with nicardipine 20?g?kg -1 in group Ⅱ Atracurium was given at the dose of twice ED 95 (05mg?kg -1) in groupⅢ and combined with nicardipine in group Ⅳ Anaesthesia was maintained with 50% nitrous oxide in oxygen and fentanyl in all groups Neuromuscular function was monitored with Biometer accelographResults The onset time of vecuronium was shortened from 26 min (groupⅠ) to 20 min (groupⅡ) (P

Objective To explore the clinical value of depression and event related potentials P300 in children with narcolepsy(NRL).Methods Event related potentials P300 test and self-rating depressive scale(SDS)test were performed in 39 children with NRL and 38 normal healthy children were selected as controls.Denmark Keypoint 4 path myoelectricity induced potential equipment and short sound stimulus were applied to determine event related potentials P300 test.Participants were required to hit the key for target stimulus,and reaction time and percentage of hits were recorded automatically.Long P300 and(or) low P3 amplitude of wave exceeded control group 2.5 s were abnormal.Zung SDS was adopted to evaluate somatization disorder,psychomotor disturbance,depression obstruction factor value.SPSS 12.0 software and t test were done to analyze the data.Results The abnormal rate of P300 in children with NRL of children group was 82.1%(32/39 cases),the latency of N2(260.7?22.1)ms and P3(353.1?25.9) ms of event related potentials of children with NRL group were longer than those in control group(241.0?23.2)ms,(341.3?26.1)ms,and there was significant difference between two groups(t=2.77,4.82 Pa

Objective To investigate the relationship between the expressions of VEGF,ENS, MVD and arteriosclerosis (AS) plaque in atherosclerosis obliterans (ASO) patients. Methods Immunohistochemical staining was used to determine the expression of VEGF, ENS and MVD value in the atherosclerosis plaque of ASO patients hospitalized from October 2007 to August 2010 in our hospital (n =30),and the femoral arterials of control were collected from 22 amputation after traumatic injury. Results The expressions of VEGF (2. 24 ±0.31 vs. 1.87 ±0.27,t =3.58),ENS (1.84 ±0.41 vs. 1. 56 ±0. 38, t =2.09) and MVD value (8.79 ±2.46 slip/HP vs. 6. 05 ±1.68 slip/HP, t =3.64) were significantly higher in the AS plaque of ASO patient than control (P <0.01 or P < 0. 05). We found positive correlations between the expression of VEGF, VEGF/ENS with MVD (r = 0. 391, P < 0. 01; r =0. 583, P < 0. 05) , while negative correlation between the expression of ENS with MVD (r=-0. 328,P <0. 05). Conclusion VEGF promotes the angiogenesis in atherosclerosis plaque,while ENS shows opposite effect The comprehensive performance of them is promoting angiogenesis.

AIM: To evaluate the diagnostic accuracy of macular ganglion cell - inner plexiform layer ( GCIPL ) measurements using high- definition optical coherence tomography ( Cirrus HD - OCT ) ganglion cell analysis algorithm for detecting early and moderate to severe glaucoma.
METHODS:Twenty normal control persons, 26 patients with early glaucoma and 29 patients with moderate to severe glaucoma were enrolled in this study. Macular GCIPL, optic nerve head ( ONH ) parameters and peripapillary retinal nerve fiber layer ( RNFL ) thickness were measured in each subject. Then all measured results of each parameter were calculated using SPSS17. 0. Areas under the receiver operating characteristic curves ( AUC) of each parameter were calculated to compare the diagnostic accuracy for detecting early and moderate to severe glaucoma.
RESULTS: For detecting early glaucoma, AUC of average RNFL and seven clock value of RNFL were the biggest ( 0. 871 and 0. 896 respectively ), the AUC of parameters in GCIPL were also significant, among them,
the average GCIPL showed bigger AUC(0. 847) than the minimum GCIPL (0. 812). For diagnosing moderate to severe glaucoma, the AUC of rim area was 0. 992, which was bigger than that of average RNFL ( 0. 991 ). The minimum GCIPL showed bigger AUC ( 0. 983 ) than the average GCIPL (0. 967). For early glaucoma diagnosis, the sensitivity of average RNFL was the highest (76. 9%), while the average GCIPL has the highest specificity (93. 5%).
CONCLUSION: AS a new diagnostic parameter for detecting glaucoma, GCIPL shows similar diagnostic potential compared with RNFL. For early glaucoma diagnosis, average RNFL is the most important parameter, while screening early glaucoma, average GCIPL should be paid more attention.

Objective To observe the effect of magnesium valproate sustained release tablets on children with epilepsy and its effects on cognitive function.Methods Magnesium valproate sustained release tablets were conducted on 38 cases.Close attention was paid to both the degree of paroxysm control and side effects during treatment while periodic examinations on liver function and blood routine were also conducted.The intelligence and P300 of children with epileptics were respectively measured before and after 6-month treatment.Forty children of control group was set up.Results Eighteen cases were totally under controlled(47.4%),11 obviously effect(28.9%),6 effect(15.8%).The total effective rate in total was 92.1%.Obvious differencees in intelligence between children with epileptics and control group before and after 6-month treatment were observed(all P0.05).Conclusions Magnesium valproate sustained release tablets is a new type of broad-spectrum anti-epileptic drug,which has an obvious effect on treatment of children with epileptic without any obvious adverse reaction.It imposes little influence on children′s cognitive function.

In this paper, the popular domestic varieties and quality standard of Scrophulariaceae plants used in Tibetan medicine were analyzed. The results showed that there were 11 genera and 99 species (including varieties), as well as 28 medicinal materials varieties of Scrophulariaceae plants were recorded in the relevant literatures. In relevant Tibetan standards arid literatures, there are great differences in varieties, sources, parts, and efficacies of medicinal plant. Among them, about 41.4% (including 41 species) of endemic plants, about 15.2% (including 15 species) of the original plants have medicinal standard legal records, except the medicinal materials of Scrophalaria ningpoensis, Lagotis brevituba, Picrorhiza scrophulariiflora, Veronica eriogyne general, most varieties have not completed quality standard. Consequently it is necessary to reinforce the herbal textual, resources and the use present situation investigation, the effects of the species resources material foundation and biological activity, quality standard, specification the medical terms of the plants, and promote Tibetan medicinal vareties-terminologies-sources such as the criterion and quality standard system for enriching the varieties of Tibetan medicinal materials and Chinese medicinal resources.
Drugs, Chinese Herbal ; chemistry ; pharmacology ; Humans ; Medicine, Tibetan Traditional ; standards ; Plants, Medicinal ; chemistry ; classification ; Scrophulariaceae ; chemistry ; classification

In this paper, the domestic varieties and quality standard of Leguminosae medicinal plants used in Tibetan medicine were analyzed. The results showed that there were 36 genera and 142 species (including varieties), as well as 64 medicinal materials varieties of Leguminosae plants were recorded in relevant literatures. In relevant Tibetan standards and literatures, there are great differences in varieties, sources, used parts, and efficacy of medicinal plants. Among them, about 38.0% (including 54 species) of the endemic plants, about 25.4% (including 36 species) of the original plants have medicinal standard legal records, except 9 kinds of traditional Chinese medicine general quality standard more fairly completed, the most varieties have only description about characters, identification, etc. Therefore it is necessary to reinforce study for the herbal textual, resources and the use present situation, chemical components and biological activity, quality standard, medicinal terms specification, to promote establishment of quality standard system for variety-terminologies-sources of Tibetan medicinal plants.
Fabaceae ; Medicine, Tibetan Traditional ; standards

ObjectiveTo evaluation the intervention effects of hand hold hands method in brick tea borne fluorosis.MethodFour to six grade pupils were selected in brick tea borne fluorosis areas,and health education knowledge questionnaire investigation was conducted.Drinking tea related health behaviors were also surveyed by the method of questionnaire.Then health education lecture on brick tea borne fluorosis was carried out,and health education material and low fluoride brick tea were provided to the pupils,the students were also requested to tell their parents brick tea related knowledge and supervise them to get rid of harmful drinking tea behavior,and cultivate healthy lifestyle.One year later pupils' health knowledge questionnaire and parents health related behavior questionnaire were carried out once again.The knowledge awareness rates of students before and after the intervention were compared.Health related behavior change rates of the parent between different ages and education levels were compared,and the implementation rates of different brick tea related behaviors before and after the intervention were compared.ResultsAfter 1 year of health education,the student's knowledge awareness rate on brick tea fluorosis was [94.3％ (33/35)],compared with the rate before intervertion [14.3％ (5/35)] increased significantly(X2 =46.111,P ＜ 0.01 ).The overall behavior change rate of health related behavior was 68.6％.After the intervention,the implementation rate of whether drinking tea,drinking overnight tea,drinking boiled for a long time tea decreased(P ＜ 0.01).But the behavior implementation rates of drinking tea with milk,drinking and buying low fluoride brick tea significantly increased(P ＜ 0.05).Implementation rate of whether drinking brick tea did not change significantly(P ＞ 0.05).ConclusionsThrough hand hold hands health education intervention,the knowledge awareness of students on brick tea fluorosis increases,and their parents can actively change bad tea drinking habits,and cultivate healthy lifestyle.

Objective To investigate the method and value of adjustable interatrial fistulization in the operation of congenital heart disease(CHD) accompany with severe pulmonary arterial hypertension(PH).Methods Twenty-seven patients(19 male,8 females) accompany with severe PH were entered the study,age ranged from 4 to 14 years old,weight from 13.7 to 42.0 kilogram.The enrolled diseases included 11 cases of atrial septal defect(ASD),10 cases of ventricular septal defect(VSD),4 cases of patent ductus arteriosus(PDA),and 2 cases of Ebstein syndrome accompany with severe tricuspid insufficiency.All patients were diagnosed as CHD accompany with severe PH(bidirectional shunt)which was the contraindications for routine operation before operation through chest X-ray,electrocardiography,ultrasonic cardiography,cardiac catheteri-zation and cardiac angiography.Results With adjustable interatrial fistulization and treatment to the abnormalities,14 fistulaes were closed immediately after operation,7 fistulaes were closed 2 days after operation,3 fistulaes were closed 3 days and 1 fistulae was closed 4 days after operation and accompanied with empyema discharged initiatively.One fistula was never closed,1 case died from low cardiac output symptom.The effective rate was 92.6%,closed to that of routine operations.Conclusion Adjustable interatrial fistulization is an easy procedure,and it can decrease the danger of PH post-operation effectively and provide operation opportunity for those patients with CHD approaching terminal stage.

OBJECTIVE: To study the expression of substance P (SP) in human sudden erethistic death, and to seek objective morphological supports to diagnose sudden erethistic death for forensic medicine. METHODS: The expression of SP was detected with immunohistochemical technique on 15 human laryngopharynx and gastrointestine of sudden erethistic death, and 20 sudden death of heart attack as control. The images of SP were analyzed by image analyzer, and the positive indexes (PI) were calculated. RESULTS: SP expression in the experimental groups was significantly stronger than that in the control one (P < 0.001). CONCLUSION SP expression can offer an objective morphological reference support for forensic diagnosing sudden erethistic death.
Adolescent ; Adult ; Anaphylaxis/pathology* ; Autopsy ; Child ; Death, Sudden ; Death, Sudden, Cardiac/pathology* ; Female ; Gastric Mucosa/metabolism* ; Humans ; Immunohistochemistry ; Intestinal Mucosa/metabolism* ; Male ; Middle Aged ; Mucous Membrane/metabolism* ; Pharynx/metabolism* ; Substance P/metabolism* ; Young Adult