To a better understanding for molecular mechanism of oncogenesis in hepatoma, primary hepatocellular carcinoma and hepatoma cell lines (Hep 3B, PLC/PRP/5, Hep G2) were subjected to detailed cytogenetic analysis with G-banding method after cell cultures. No cloned chromosomal abnormalities were found in the primary hepatoma (below 100%). On the other hand, all hepatoma cell lines were cloned, the specific chromosomal abnormalities in Hep 3B were del(1p21), del(6q14) and t(1 ; 11)(pll ; q13). Genes of AMY1A, CGA, SEA and HSTF1 were located on 1p21 and 6q14 respectively. SEA and HSTF1 were located on 11q13. Regions of chromosome abnormalities in PLC/PRF/5 were the same found in Hep 3B. Besides, del(1q32) and del(1p32) were also cloned. Gene of CR1 and MYCL1 were located on 1q32 and 1p32 respectively. The characteristic findings of chromosome abnormalities in Hep G2 were del(1p31) and del(1q22). And GST1 and DAF were located on these regions each other Del(6q11) and del(1p22) were also found in Hep G2. From the above results, it is presumed that HBV may integrate to AMY1A gene or near this gene and leads to loss of functions to this gene. And impaired regulation of CGA occurs in next step. SEA, HSTF1 and MYCL1 oncogenes may act as a progressing factor of tumourgenesis in HBsAg(+) hepatoma. Some factors like chemical agents may cause functional loss of GST1 and DAF at first and functional loss of cell regulation of CGA occurs in next step. SKI oncogene may promote the progression of carcinogenesis in this cell line. Whether any causative agents are involved in carcinogenesis of hepatoma, functional loss of CGA gene is the most important factor in tumour-genesis in hepatoma.
Carcinogenesis ; Carcinoma, Hepatocellular* ; Cell Culture Techniques ; Cell Line ; Chromosome Aberrations* ; Clone Cells ; Cytogenetic Analysis ; Hand ; Humans* ; Methods ; Oncogenes

No abstract available.

Computed tomography(CT) has enabled early recognition and treatment of focal injuries in patients with head trauma. However, CT has been less beneficial in identifying diffuse brain injury(DBI). The authors have analyzed retrospectively, a series of 132 patients with OBI observed for 2 years from Aug. 1986 to Jul. 1988 to evaluate the significance of the factors affecting outcome. Eighty-three patients were selected as being compatible with moderate and severe diffuse axonal injury(DAI) classified by Gennarelli, defined by coma without a CT lesion that is an obvious cause and coma greater than 24 hr with or without decerebration. The results are summarized as follows: 1) The 38(45.7%) out of 83 patients were found below age of 20, but there was no statistical significance between age distribution and outcome. 2) In case of initial Glasgow coma scale(GCS) of 7 or 8, 32(86.5%) out of 37 patients revealed good outcome, but 18(90%) of 20 patients with a score of 3 or 4 revealed poor outcome(p<0.01). 3) With regard to brain swelling in CT, there was significant statistical difference to outcome(p<0.05). 4) Small hemorrhages on corpus callosum, basal ganglia, basal cistern, peritentorial, lateral ventricle that is characteristic CT findings for DAI were showed 58(70%) out of all cases. It might be concluded that initial GCS, brain swelling and small hemorrhages in CT were significant factors affecting outcome in DAI.
Age Distribution ; Axons ; Basal Ganglia ; Brain ; Brain Edema ; Brain Injuries* ; Coma ; Corpus Callosum ; Craniocerebral Trauma ; Diffuse Axonal Injury ; Hemorrhage ; Humans ; Lateral Ventricles ; Retrospective Studies ; Tomography, X-Ray Computed

The purpose of this study was to compare the accuracy of currently used elastomeric impression materials for complete arch impression taking. Five elastomers (Impregum, Permlastic, Express, Extrude, Examix) and one Irreversible hydrocolloid (Aroma-fine) were tested. For each material, 5 impressions were made of stainless steel model to which five tapered posts were attached. Custom trays were used for polyether and polysulfide impression materials, and putty/wash two step technique was used for addition polyvinylsiloxane impression materials. Improved stone models were poured to all impressions. Accuracy of the materials was assessed by measuring ten distances on stone dies poured from impressions of the master model. All measurements for master and improved stone models were with three dimensional measuring machine. The results were as follows 1. The dimensional accuracy of polyether, extrude, and examix were significantly superior to polysulfide, exress, and alginate in reproducing full arch mode (p<0.05). 2. There were no statistical differences in dimensional accuracy for full arch impression between polyether, extrude and examix (p>0.05). 3. there were no statistical differences in dimensional accuracy between polysulfide, express, and alginate (p>0.05). 4. There were no statistical differences between addition polyvinyl siloxane materials (p>0.05). 5. There were no statistical differences between anterior-posterior and lateral dimensional changes of all impression materials (p>0.05).
Colloids ; Elastomers* ; Polyvinyls ; Siloxanes ; Stainless Steel

Xeroderma pigmentosum, a rare, genetically determined dermatosis, is often cornplicated by malignant chagnes of various types-most commonly, basal-cell epithelioma, squamous cell epithelioma, less frequently malignant meIanoma, keratoacanthoma, fibrosarcoma, and endothelioma have been reported. In Korea, no case has ever been reported in literature except four cases at a symposium for dermatology, two of them complicated by basal-cell epithelioma, and the others by squamous cell epithelioma. This case reported was complicated by squamous cell epithelioma. As one of the anticancer treatments, especially for skin cancer, Bleomycin is, now, drawing the attention for its high therapeutic index with only few side effects. For this case, we injected Rleomycin 15mg at once, twice a week, for 8 weeks, So total injection was 16 times and 240mg of Bleomycin was given in total. While this amount given for this case was not enough for the complete treatment of squamous cell epithelioma, we got the following results: 1. Clinically, the ulcerated lesion of the cancer on the right ala nasi had shrunken in size with reepithelialization at the margin showing clear surface, and 2. microscopically, the atypical cancer cell masses and individual cell keratinizations had been decreased in size and in number, and less remarkable comparing to those in untreated stage at his first visit to the hospital.
Bleomycin* ; Carcinoma* ; Dermatology ; Fibrosarcoma ; Keratoacanthoma ; Korea ; Skin Diseases ; Skin Neoplasms ; Ulcer ; Xeroderma Pigmentosum

Immunohistochemical study was performed for CEA staining patterns and PCNA indices. And the relationship between immunohistochemical findings and well-known clinical prognostic factors on the purpose of the clinical usefulness was evaluated. In forty seven cases of surgically removed colorectal carcinomas, the results were as follows; CEA staining patterns were apical (17 cases) and cytoplasmic (30 cases) type. Carcinomas with cyto plasmic pattern for CEA revealed more advanced Dukes' stage and more undifferentiated type and higher incidence of lymph node metastasis and were correlated with increased serum CEA levels. But PCNA indices showed no correlation with the Dukes' stage, histologic grade and CEA staining patterns. The cytoplasmic pattern of CEA immunohistochemistry may be a useful marker suggesting more aggressive biologic behavior of the colorectal carcinomas.
Colorectal Neoplasms* ; Cytoplasm ; Immunohistochemistry ; Incidence ; Lymph Nodes ; Neoplasm Metastasis ; Proliferating Cell Nuclear Antigen*

PURPOSE: Kimura's disease is an inflammatory condition of unknown etiology which usually occurs in salivary glands and cervical lymph nodes. This study was undertaken to evaluate the radiologic characteristics of Kimura's disease using CT and MRI. MATERIALS & METHODS: We evaluated CT & MR of 10 patients with histologically proved Kimura's disease in the head and neck. Six males and four females between 16 and 58 years old (mean age, 37.6 years) were included. Contrast enhanced CT scan was performed in all, and spin-echo MRI was performed in 2 patients. RESULTS: Both CT and MRI demonstrated the distinctive sites of involvement that were major salivary glands (4 cases) and lacrimal gland (1 case), periglandular soft tissue (8 cases), and cervical lymph nodes (8 cases). Majority of soft tissue lesions were ill-defined with infiltration to adjacent fascial planes, but nodal lesions were relatively well-defined and homogenous. The lesions were iso-intense on Tl-weighted image and hyper-intense on T2-weighted image. All but a few nodal lesions showed variable degree of contrast enhancement on CT and MRI. CONCLUSION: In diagnosis of Kimura's disease, CT & MRI of head & neck, especially salivary glands, are useful. In cases of recurrent periglandular soft tissue mass with contrast-enhancing cervical lymphadenopathy in adult patients, Kimura's disease should be included in the differential diagnosis.
Adult ; Diagnosis ; Diagnosis, Differential ; Female ; Head* ; Humans ; Lacrimal Apparatus ; Lymph Nodes ; Lymphatic Diseases ; Magnetic Resonance Imaging ; Male ; Middle Aged ; Neck* ; Salivary Glands ; Tomography, X-Ray Computed

A clinical review was performed on 64 cases of the foot drop treated by transferof the Posterior Tibialis tendon to the dorsum of the foot at Taegu Presbyterian Hospital during the period from January, 1965 to December, 1971. The follow-up result of 42 cases were obtained from 13 weeks to 4 years and 8 months. The result are summarized as follows. 1. Among 63 patients, there were 46 males and 17 females, ranging 12 to 57 years old of age. 2. The cause of foot drop were peroneal nerve palsy due to leprosy in 61 cases, anterior poliomyelitis in 2 cases, and traumatic peroneal palsy in 1 case. 3. Of 42, cases which follow-up study was done, the degree of active dorsiflexion of the ankle were 90 degree or more in 29 cases (69.0%) and range of active movement was 25 degree or more in 31 cases (73.8%). 22 cases (52.4%) had active dorsiflexion of the ankle which was 90 or more in degree and range of active movement of the ankle which was 25 degree or more. 4. Iterosseous route was used in 26 cases and circumtibial route in 38 cases. Their end results revealed slight better in the latter method. 5. The major causes of complication were detachment at insertion site in 7 cases (53.8% of total failure). weak motor power of the Posterior Tibialis in 4 cases, and adhesions in 2 cases.
Ankle ; Daegu ; Female ; Follow-Up Studies ; Foot ; Humans ; Leprosy ; Male ; Methods ; Paralysis ; Peroneal Nerve ; Poliomyelitis ; Protestantism ; Tendons

Hemolytic disease of newborn due to Rhesus incompatibility occurs mostly by anti-Rh(D) antibodies. But recently, because of Rh(D) immune globuin prophylaxis, Rh(D) incompatibility is decreased and importance of minor group incompatibility is increasing. The majority of minor blood group incompatibilities are anti-c, anti-E or anti-Kell, but hemolytic diseases caused by combination of these antibodies are relatively rare. The 21 day-old male neonate was admitted because of pallor and poor feeding for 2 days. Laboratory data showed severe normochromic and normocytic anemia with increased reticulocyte and bilirubin. Patient's and his mothers blood type was B, Rh(D) positive. Minor blood groups of mother's and sons were different and anti-c and anti-E antibody were detected in mother's serum. After transfusions of two times with anti-c and anti-E free B, Rh(D) positive blood, the baby was discharged at 11th hospital day with good general condition.
Anemia ; Antibodies ; Bilirubin ; Blood Group Antigens ; Blood Group Incompatibility ; Erythroblastosis, Fetal ; Humans ; Infant, Newborn* ; Male ; Mothers ; Pallor ; Reticulocytes

No abstract available.