1.Successful Endovascular Treatment of an Infected Aortic Aneurysm Induced by Klebsiella pneumoniae
Hong Gwon BYUN ; Yook KIM ; Jung Hwan LEE ; Jisun LEE ; Kil Sun PARK
Journal of the Korean Radiological Society 2020;81(3):733-738
Aortic aneurysms infected by Klebsiella pneumoniae are rarely seen. We describe a 50-year-old man with infected aortic aneurysm that was successfully treated with endovascular aneurysm repair (EVAR). Diagnosis was confirmed using blood culture and computed tomography (CT). Intravenous antibiotics were immediately administered, with improvements in clinical findings and negative blood cultures before the procedure. Twenty-four months after the procedure, the patient was stable and serial CT revealed regression of the infected aortic aneurysm. Therefore, after controlling bacteremia and fever with targeted antibiotic therapy, EVAR can be considered as an alternative for patients who have serious comorbidities and are ineligible for conventional surgery.
2.The Effects of Aldosterone and Cytokines IL-1beta, TNF-alpha on the Expression of Angiotensin Converting Enzyme Gene in Vascular Smooth Muscle Cells.
Duk Kyung KIM ; Jeong Eun HUH ; Yoon Hyuk CHOE ; Sun Jin PARK ; Eun A JUNG ; Jonghoe BYUN ; Hyeon Cheol GWON ; Seung Woo PARK ; June Soo KIM ; Sang Hoon LEE ; Kyung Pyo HONG ; Jeong Euy PARK ; Jung Don SEO ; Won Ro LEE
Korean Circulation Journal 1999;29(1):84-95
BACKGROUND: It has been suggested that all components of the renin-angiotensin-aldosterone system (RAAS) are present in the vascular wall and that the vascular RAAS modulates vascular tone and vascular hypertrophy. One of the catalytic step in the RAAS cascade is the local conversion of angiotensin I to angiotensin II (Ang II) by angiotensin converting enzyme (ACE). One of the major sources of ACE in the vasculature is vascular smooth muscle cells (VSMC). Here, we provide insight into the intrinsic mechanisms by which the components of RAAS regulate gene expression of ACE in cultured smooth muscle cells of the rat and we also investigated the effects of cytokines on ACE mRNA. METHODS: RNA was extracted from the primary cultured VSMCs. We analyzed the expression levels of ACE by competitive reverse transcription-PCR using recombinant RNA as an internal standard. RESULTS: 1) ACE mRNA level was increased markedly by aldosterone in a dose- and time-dependent manner, indicating that there exists positive feedback mechanism within RAAS. 2) The induction of ACE mRNA by aldosterone was inhibited by spironolactone. 3) Aldosterone-stimulated expression of ACE was also inhibited by Ang II, which shows that Ang II acts as a negative regulator of the expression of ACE in RAAS cascade. 4) Interleukin-1beta or TNF-alpha did not induce ACE mRNA expression. 5) However, mixture of interleukin-1betaand TNF-alpha(CytoMix) significantly increased the expression of ACE. It was also shown that CytoMix increased aldosterone-stimulated ACE mRNA expression in an additative manner. CONCLUSION: These results indicate that the expression of ACE in smooth muscle cells is modulated by the components of RAAS and cytokines. The intrinsic positive and negative feedback controls of RAAS would play an important role in the pathogenesis of vascular diseases.
Aldosterone*
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Angiotensin I
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Angiotensin II
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Angiotensins*
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Animals
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Cytokines*
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Gene Expression
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Hypertrophy
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Interleukin-1beta
;
Muscle, Smooth, Vascular*
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Myocytes, Smooth Muscle
;
Peptidyl-Dipeptidase A*
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Rats
;
Renin-Angiotensin System
;
RNA
;
RNA, Messenger
;
Spironolactone
;
Tumor Necrosis Factor-alpha*
;
Vascular Diseases
3.Radiofrequency Ablation of the Gastrointestinal Tract with a Stent-Like Electrode: Experimental Study.
Joo Nam BYUN ; Sung Gwon KANG ; Jeong Yeol CHOI ; Dong Hyun KIM ; Jung Min LEE ; Young Chul KIM ; Keun Hong KI ; Chul Gap LEE
Korean Journal of Radiology 2003;4(1):19-26
OBJECTIVE: To assess the technical feasibility of a newly designed stent-like electrode in rabbits. MATERIALS AND METHODS: A stent-like electrode was knitted from a single thread of nitinol wire and interconnected to a generator using similar wire. In order to gauge the extent of radiofrequency ablation (RFA), we measured the depth of the ablated area in cow liver using a combination of 180-sec time intervals and 20-watt power increments. For data processing, Cox regression analysis was used. RFA was also applied to the small intestine of rabbits using this stent-like electrode under six different sets of conditions: 10 watts for 1 min, 10 watts for 2 mins, 20 watts for 1 min, 20 watts for 2 mins, 30 watts for 1 min, and 30 watts for 2 mins. To determine the gross and microscopic findings, six animals were sacrificed immediately after the procedure and the results obtained under the different sets of conditions were correlated. Eight rabbits were monitored for 4 weeks prior to sacrifice. RESULTS: For both ex-vivo and in-vivo ablations, the depth of the thermal lesion showed linear correlation with both the duration of RFA and the power applied. RFA of the duodenum was technically successful in all 14 rabbits. The acute changes occurring in the rabbits' small intestine included color change, cytoplasmic denaturation, fibrin deposition and hemorrhage, among which hemorrhage of the mucosal layer was the earliest finding. RF ablation for 2 mins at 30 watts caused serosal hemorrhage. The gross and histologic changes occurring showed close correlation under all six sets of conditions. CONCLUSION: Use of the stent-like electrode proves technically feasible but to determine the nature of the chronic change occurring in the gastrointestinal tract after RF ablation, further investigation and long-term follow-up in animals are required.
4.Gene Transfer into Cultured Cardiac Myocytes Mediated by Retrovirus.
Jeong Eun HUH ; Duk Kyung KIM ; Jong Hoe BYUN ; Sun Jin PARK ; Eun Suk JEON ; Yoon Hyuk CHOE ; Eun A JUNG ; Hyeon Cheol GWON ; Seung Woo PARK ; June Soo KIM ; Sang Hoon LEE ; Kyung Pyo HONG ; Jeong Euy PARK ; Francois Loic COSSET ; Jung Don SEO ; Won Ro LEE
Korean Circulation Journal 1999;29(2):182-191
BACKGROUND: Transplantation of cardiac myocytes (CMs) into the injured heart emerges as a potential alternative for the treatment of heart failure. Genetic modification of CMs could enhance and/or modify its therapeutic effects. The characteristics of retroviral gene delivery, which is most commonly used in human trial, has been minimally studied in CMs due to its low efficiency in non-dividing cells. In this study, using newly developed high-titer retrovirus, we evaluated 1) the efficiency of gene transfer into CMs, 2) whether S phase during infection is necessary for the transduction, and 3) characteristics of gene delivery to mononucleated vs binucleated CMs. METHODS: Enriched CMs were cultured from the ventricles of 1 day-old rat hearts. The cells were transduced by MFG-nls-LacZ retroviruses (5x107 IU/ml) in the presence or absence of polybrene. 3H-thymidine was added to label cells in S phase. The cells were stained for
Animals
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Autoradiography
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Cells, Cultured
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Dichlorodiphenyldichloroethane
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Genetic Therapy
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Heart
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Heart Failure
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Hexadimethrine Bromide
;
Humans
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Myocytes, Cardiac*
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Rats
;
Retroviridae*
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S Phase
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Thymidine
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Zidovudine