The family of HSP60 belongs to heat shock proteins with highly species conservatism and some important biologic functions. It can help other proteins for their assembling, folding and translocating, and plays a role in protecting cells against injuries and other types of stress. In addition, HSP60 is frequently recognized by the immune system as predominant antigens during infections and the progression of certain autoimmune diseases and might provide a novel strategy for the development of immunotherapeutics. This review focuses on distribution, molecular chaperone mechanism, function and gene expression regulation of HSP60. [

Objective To determine the predictive value of central venous oxygen saturation (ScvO2) in volume response by comparing the relationship between variation of ScvO2 and cardiac output (CO) variability before and after volume expansion in elderly patients with septic shock.Methods Forty-five patients who diagnosed septic shock were enrolled in the study,inserted CVC and were carried out continuous cardiac output monitoring(PICCO) before volume expansion.Every patient was treated by rapid intravenous infusion of 250 mL physiological saline,then take blood gas analysis before and after treated which the blood samples were collected from central venous catheter.The value of ScvO2 and the value of cardiac output was recorded.Degree of variation between two sets of data were tested by Student t-test.The correlation was determined by using Pearson's correlation test.Operating characteristics curve analysis was used to test their ability to distinguish R and NR.Results The mean arterial pressure (MAP)、heart rate(HR)、CO、ScvO2 were demonstrated and have statistical significance (P＜ 0.05) compared with before treated.CO and ScvO2 indexes were positively correlated (P＜0.01) before and after volume expansion.ScvO2 variations after VE were significantly correlated with CO changes after VE (r=0.781,P＜0.01).Conclusion ScvO2 dynamic changes can be used as a criterion for determining the fluid responsiveness.

By combining WGA-HRP retrograde tracing and immunohistochemical methods,the characteristics of the vasopressin(VP)-like immunoreactive neurons, which project to the posterior pituitary were studied. They were located subependymally, and predominately along:(1) the dorsal wall of the third ventricle at the preoptic area and posterior magnocellular paraventricular nucleus levels, (2) the ventral wall of the third ventricle at the suprachiasmatic nucleus level, and (3) at the floor of the third ventricle. Four types of the cells could be distinguished. They were found to squeeze in between the ependymal cells, bring themselves very close to the cerebrospinal fluid (CSF),or to be situated in the subependymal layer, sending some of their processes into the ependyma. The double labelled neurons were also detected subpially in the supraoptic nucleus (SON) and the retrochiasmatic nucleus. A large number of dendrites from the neurons in the SON extended toward the CSF in the subarachnoidspace. In summary, the VP-like immunoreactive neurons which projected to the posterior pituitary were very close to the CSF. It is, therefore, proposed that these neurons may monitor changes in the CSF and adjust the secretion of VP in the posterior pituitary. They may also release VP directly into the CSF.

The periventricular oxytocin(OXT)-immunoreactive neurons of the 3 third ventricle in the rat were studied under electron microscope by using pre-embedding PAP immunocytochemistry. Special attention was payed to the relationship between OXT-containing neurons and ependymal cells and the synaptic architecture of these neurons in the periventricular neuropil. The results revealed that the positively immunostained cell bodies and larger, dendrites of neurons were just beneath ependyma and contacted directly with ependymal cells. Typical Gray Ⅰ type or untypical axon-dendrite synapses were found between the immunolabelled neurons and unlabelled axon terminals. A cross section of a immunoreactive process was seen on the luminal surface of ependymal cells in one case. In the subependymal neuropil many synapses formed by OXT-immunoreactive dendrites and non-immunoreactive axon terminals which contained different kinds of synaptic vesicles were encountered. It is suggested that the subependymal neurons may be important in signal integation between cerebrospinal fluid and neuronal inputs. It is also possible that the subependymal neurons may release peptides,i, e. oxytocin into CSF.

Objective To determine CD+4CDnull28 T cells and their relationship with the microinflammatory state and left ventricular hypertrophy in end-stage renal disease (ESRD) patients.Methods One hundred and twenty ESRD patients were selected,including sixty non-dialytic stage 5 chronic kidney disease patients(CKD5 stage) and thirty maintenance dialysis patients(CKD5D stage).In addition,thirty healthy adults from the hospital medical center were selected as the control group.Flow cytometry was used to detect the frequencies of T lymphocyte subsets in peripheral blood.All enrolled subjects underwent Doppler echocardiography investigations.Results(1)Compared with the control group,the high sensitivity C-reactive protein and the frequency of CD+4CDnull28 T cells were significantly expanded in CKD5 and CKD5D paients (F=14.887,9.659,all P<0.05).(2)In ninety ESRD patients,the incidence of LVH increased in CKD5 and CKD5D patients (76.67% vs.86.67%).(3)Linear correlation in CKD5 patients showed that:LVMI was positively correlated to duration of hypertension,SBP,P3+,lg(CRP+1),lgiPTH,lg(CD+4CDnull28+1) (r=0.332,0.291,0.269,0.310,0.304,0.396,all P<0.05),as well as negatively correlated to Hb(r=-0.285,P<0.05).Multiple linear regression analysis showed that LVMI was independently associated with lg(CD+4CDnull28+1) and duration of hypertension(F=6.589,P<0.000).ConclusionThere is microinflammatory state in the ESRD patients regardless of whether or not they have dialysis treatment,and they have the high incidence of LVH and exhibit a substantially increased frequency of circulating CD+4CDnull28 T cells.The frequency of CD+4CDnull28 T cells and duration of hypertension are the independent predictors of LVH in non-dialytic stage 5 CKD patients,indicates that these cells may play a critical role in the development of cardiovascular disease.

The studies on early detection of cerebral palsy(CP) showed that neurological examinations(Vojta Posture Reflex, Infant Movement Milestone, Neonatal Behavioral Neurological Assessment, General Movement Assessment), imaging examinations (cranial B-ultrasonography, CT, MRI) and electrophysiologic examinations (Brainstem auditory evoked potentials, electroencephalogram, somatosensory evoked potentials) are in common use. General Movement Assessment was used in China three years ago, showing high sensitivity and specificity in predicting CP. Imaging examinations and electrophysiologic examinations can find organic diseases and other dysfunctions.

Humans ; Lung Neoplasms ; blood supply ; pathology ; Lymphatic Vessels ; pathology ; Neovascularization, Pathologic ; metabolism ; Vascular Endothelial Growth Factors ; chemistry ; metabolism ; physiology

Adolescent ; Adult ; Child ; Child, Preschool ; China ; epidemiology ; Hepatitis B ; epidemiology ; prevention & control ; Hepatitis B Vaccines ; immunology ; Humans ; Immunization Schedule ; Infant ; Middle Aged ; Prevalence ; Vaccination ; Vaccines, Synthetic ; immunology

Objective To investigate the synergistic effect of calcium channel blocker on cyclosporine-induced gingival overgrowth (GO). Methods 130 renal transplant patients treated with cyclosporine were divided into group A (with calcium channel blocker) and group B (without calcium channel blocker). Demographic, pharmacologic and periodontal data were recorded. The prevalence and severity of GO were compared between the two groups. Three calcium channel blockers, including nifedipine, amlodipine and felodipine, were administered in the patients of group A. The relationships between these three calcium channel blockers and the prevalence of GO were analyzed. Results The patients receiving calcium channel blocker showed significantly higher prevalence of GO (44/73,60 % ) than those without calcium channel blocker (22/57, 39 %) (P＜0. 05). A higher proportion of mild GO (37 %) in group A was also observed than in group B (19 %, P＜0. 05). There were no significant differences in the proportions of moderate and/or severe GO between the two groups (P＞0. 05). Periodontal variables, including plaque index and papilla bleeding index, were significantly higher in GO patients than in those without GO in both two groups (P＜0. 05). In addition, the prevalence of GO in patients receiving nifedipine (77 %) was higher than in those receiving amlodipine (57 %) or felodipine (50 %). Conclusion The combination with calcium channel blocker is a risk factor of cyclosporine-induced GO and the use of nifedipine should be avoided for these at-risk patients.

0.05).However,body mass index and hemoglobin level were significantly higher in the slow coronary flow group compared with the control group(26.9?3.2 kg/m2 vs.24.9?2.9 kg/m2,P