Objective: To use three dimensional model in vitro to mimic solid tumor growth in vivo and explore the relationship between the multicellular resistance of colon carcinoma cell and the over-expression of cell adhesion molecules (integrin α V and integrin β3). Methods: The colon carcinoma HT-29 multicellular spheroids (MCS) model were constructed with three dimensional cell culture methods. The mRNA and protein expressions of integrin α V and integrin β3 was detected by RT-PCR and immunofluorescence analysis and compared between monolayer cells (MC) and MCS. The viability and apoptotic rate of MC and MCS were detected by flow cytometry (FCM) after treatment with 5-fluorouracil (5-FU). Results: The expression levels of integrin α V and integrin β3 in three dimensional cell culture model of HT29 MCS were much higher than those in MC (P < 0.01). Compared with MC, the sensitivity of MCS to the anticancer drug 5-FU significantly decreased ( P < 0.05), and the rate of 5-FU-induced apoptosis significantly decreased (0.346 ± 0.035 vs 0.235 ± 0.024, P < 0.05). Conclusion: Over-expression of cell adhesion molecules integrin α V and integrin β3 may increase the multi-cellular resistance of colon carcinoma cells.

Objective To study how to enhance the effectiveness of international disaster relief in order to explore the features and applications of two different patterns of medical rescue.Methods A retrospective analysis of the experiences of the China International Relief Team,including the constitution of the team,working hours on the disaster area,and the numbers of patients rescued per doctor per day,during the 12 occasions of international mission over the past 10 years since the team founded.Results Since the first time for international rescue operation acted in Algeria in 2003,the China International Search and Rescue (CISAR) team has taken part in 12 humanitarian relief operations which can be categorized into two patterns of medical rescue:the first pattern is integrated by search,rescue and initial medical service on site,the second one is exclusively for medical relief (the first pattern consisting of 15.4％ medical staff and 61.4％ medical staff in the second pattern).According to the former pattern,CISAR team should arrive at the stricken area of earthquake less than 3 days and withdraw after 7 - 10 days of work with about 10.8average working days.Four survivors trapped in collapsed buildings were rescued during the six international rescue operations,and 662 sick and casualties on average were treated each time.The latter pattern often carried out by CISAR team after the work of the former pattern (over 10 days after the disaster) had been done with average working time of 17.8 days.There were 6812 patients on average treated in each time.Comparison between the two patterns showed that the latter one needed more medical staff and longer working days.The data of these two patterns varies significantly. Conclusions The search and rescue pattern is different from the pattern of exclusively medical rescue,and the urban search and rescue team is integrated the search and rescue with medical service,which was proposed by the United States,because of its versatility in the stricken areas of earthquake.It gives lot of useful information to the developing countries for setting up modern relief teams.The time and mode of sending a search and rescue team or a medical team to the stricken area of disaster depend on the assessment of disaster and the requirements of the local government.The capability of the search and rescue team can be extended beyond the ruins,but the capability of the medical team can hardly extend deep enough beneath the ruins,so that the first pattern is much superior to the second one.To set up a local relief team should meet the requirement of the local government to deal with different kinds of disaster.

Aged ; Bronchogenic Cyst ; complications ; Female ; Humans ; Infection ; complications ; Mediastinal Cyst ; complications

Recent studies have demonstrated that hyperhomocysteinemia is an independent risk factor for stroke,which may cause vascular endothelial injury by multiple mechanisms,destroy blood coagulation and fibrinolytic systems,and influence on lipid metabolism.Methylenetetra hydrofolate reductase(MTHFR)is a key enzyme of homocysteine metabolic pathway in the human body.If it is insufficient or its activity decreases,it will directly result in the accumula tion of homocysteine in the human body,and callse hyperhomocysteinemia.Although a number of studies have found that MTHFR gene mutation is the main cause of MTHFR insufficient and its activity decrease,the correlation between MTHFR gene mutation and ischemic cerebral vascular disease is quite controversial now.This article reviews the relationship between MTHFR gene polymorphisms and ischemic stroke.

Objective To investigate the effect of interferon-alpha (IFN-α) on preventing pancreatic fibrosis and expression of pancreatic α-smooth muscle actin (α-SMA) and collagen type m in experimental rat model induced by diethyldithiocarbamate (DDC). Methods Forty male Wistar rats were randomly divided into three groups: normal control group, fibrotic group(PF group) and IFN-α prevention group(IFN-α group). Pancreatic fibrosis were induced by repeated intraperitoneal DDC injection. IFN-α group were treated with subcutaneous injection of IFN-α in saline, administered daily at the dosage of 105 U. Six weeks later, all the rats were sacrificed, the samples of the pancreatic tissue obtained by biopsy were submitted to histological studies. The expression of α-SMA and collagen type Ⅲ in pancreatic tissues were detected by immunohistochemistry. Results From the 4th week on, the weight of rats in PF group grew slowly, some rats 'weight even decreased, while the weight of rats in IFN-α group kept growing up slowiy, and the difference became significant at the 5th week (306.17±20.89 g vs 279.17±23.58 g, P＜0.05 ). The fibrosis score, Masson stain score, α-SMA and pancreatic collagen type Ⅲ in PF group were 2.679±0.899, 218. 713± 36. 102, 148.971±30. 686 and 88. 142±42.581, respectively, and the corresponding values in PF group were 1.952±0.219, 114.732±24.912, 77.237±9.275 and 59.952±25.498, respectively. The extent of fibrosis in IFN-α group was significantly better than that in PF group (P＜0.05). Conclusions IFN-α may inhibit the extent of fibrosis, decrease the expression of α-SMA and pancreatic collagen type Ⅲ, therefore, it may partially prevent pancreatic fibrosis induced by DDC.

Objective To evaluate an improved modification of TTC staining method for measuring myocardial in-farct size after ischemia-reperfusion in rats.Methods Twenty healthy SPF male 8-week-old SD rats were randomly divided into two groups:Group A with conventional TTC staining, and group B with the modified TTC staining method for measuring myocardial ischemia-reperfusion injury.The infarct size was caculated and the serum cTnI levels were determined.Results The infarcted myocardium was well detected in both groups A and B.There were no significant differences in the myocardial infarct sizes measured in the groups A and B (48.69 ±5.37 %vs.47.41 ±3.28%, P>0.05).There were no significant differences in the serum cTnI levels assayed in the groups A and B (4.51 ±0.88 ng/mL vs.4.70 ±0.71 ng/mL, P>0.05). But compared with the group A, the color contrast of stained myocardial slice and the distinguishing infarction area and non-infarction area were much clearer in the group B.Conclusions Our modified TTC staining technique using in vivo staining is an economic, convenient, fast and efficient method, being easy to control, time-saving and inexpensive, and enhances the staining effect in evaluating the size of myocardial ischemia/reperfusion injury more accurately.

Aim DNA vaccinating BALB/c mice with the constructed recombinant plasmid, pcDNA3, containing ROP1 gene from Toxoplasma gondii to observe the effect on the production of the cytokines, IFN- γ、 IL - 2 , and NO in the immunized mice. Methods Large-scale preparation of plasmid DNA by alkaline lysis,the DNA were injected through muscles of left leg in each mouse at the dosage of 100μg. A booster vaccine was given at the same dosage after two weeks. Control groups were injected with pcDNA3 blank plasmid and normal saline respectively. After 30,50 and 70 days of the booster injection, following tests were carried out 3 times separately :the serum IFN-γand IL-2 were detected by sandwich ELISA;the NO was detected by enzyme assay. Results The 3 times detected results of IFN-γγ、IL-2 and NO were significant higher in the vaccinated group than that of in control groups and the contents were increased with the vaccinated time prolonged. Conclusion The IFN - γγ、 IL - 2 and NO were produced by vaccinating BALB/c mice with the recombinant plasmid, pcROP1.

Objective To systematically review the myocardial protective effect of ischemic preconditioning in patients undergoing open heart surgery.Methods PubMed,EMBASE,Highwire,CENTREN and its affiliated clinical trial registration data center,CBM and CNKI were searched to identify all randomized controlled trials involving the myocardial protective effect of ischemic preconditioning in patients undergoing open heart surgery under general anesthesia.The quality of the studies was evaluated by the method recommended by Cochrane Collaboration.Evaluation indexes included the incidences of postoperative cardiac death,myocardial infarction and ventricular arrhythmias and postoperative inotropic drug requirement.Meta-analysis was conducted using the Cochrane Collaboration's RevMan 5.0 software.Results Twelve randomized controlled trials (8 high-quality trials and 4 medium-quality trials) involving 626 patients were included in our Meta-analysis.Meta-analysis indicated that ischemic proconditioning significantly reduced the incidence of postoperative ventricular arrhythmias and postoperative inotropic drug requirement (P ＜ 0.05) and had no effect on the incidences of postoperative cardiac death and myocardial infarction (P ＞ 0.05).Conclusion Ischemic preconditioning can reduce the postoperative ventricular arrhythmias and have no effect on postoperative cardiac death and myocardial infarction in patients undergoing open heart surgery.

Objective To analyze the causes of infection after cesarean section.Methods 80 patients having infection after cesarean section from Jan.2008 to Jan.2013 in our hospital were studied.The clinical data were retrospectively analyzed.The nosocomial infection-related factors were observed and related factors were ana-lyzed by Logistic regression analysis.Results 80 cases of maternal infection occurs after cesarean section.The highest rate of infection was urinary tract infection and respiratory infection, accounting for 43.75%and 31.25%respectively.We found the possible factors related to infection after cesarean section induding operative time, the number of invasive procedures, premature rupture of membranes, and the use of antibiotics.Conclusions Knowing the factors related to infection and improving the operative skills of gynecologists can help to reduce the rate of infection after cesarean section.