Objective:To explore the effects of allergic factors in chronic sinusitis . Method:Skin prick test and specific serum IgE of 194 patients with chronic sinusitis were performed in our department before endoscopic sinus surgery from October,2007 to October,2008, and correla- tion between test results and clinical classification was analyzed retrospectively. Result: ① Result of skin prick test was analyzed. Positive rate of house dust was 20.6% (40/194),of dust mites 16.0%(30/194),of house dust mites 14.9%(29/194). ② Positive rate of allergic specific serum IgE and skin prick test in experimental group was compared with that of control group. Positive rate of house dust mites,dust mites and house dust between groups were significantly different(P<0.05).③ Clinical classification of chronic sinusitis had a negative correlation with the specific serum IgE (house dust mites ,house dust and dust mites)about experimental group(P<0.05). Conclusion: Allergic factors was associated with etiopathoge-nisis and course of disease of chronic sinusitis.

BACKGROUND: Hematic protective has arisen widely attention in China and abroad due to the serious blood contamination and transfusion related diseases.OBJECTIVE: To observe hematic protective effect of hypertonic solution chloride hydroxyethy140 injection (HSH) on acute hypervolemic hemodilution (AHH) in maxilla Lefort Ⅰ osteotomy.METHODS: Totally 40 patients were randomly divided into the experimental and control groups, with 20 patients in each group. All patients were operated under tracheal intubation general anesthesia, in the experimental group, 4 Ml/kg HSH was infused with speed of 10 Ml/min, and the infusion was finished within 30 minutes. There was no treatment in the control group. Blood routine examination (including hematocrit, hemoglobin, blood platelets count) and routine coagulation test (including platelet, partial thromboplastin time, fibrinogen) was performed at the points of before AHH (T_0), at the end of AHH (T_1), 30 min after AHH (T_2),60 min after AHH (T_3), 3 hours after AHH (T_4), 1 day after operation (T_5), and 3 days after operation (T_6), respectively.Simultaneously, the capacities of blood transfusion, liquor transfusion, hemorrhage and urine during operation was recorded. RESULTS AND CONCLUSION: All the patients were included in the final analysis. Compared to the control group, the capacity of blood transfusion in the experimental group was smaller than that of the control group (P<0.05), with greater urine volume (P<0.05). However, the differences of liquor transfusion and hemorrhage had no significance (P>0.05). After controlled hypotension,the heart rates of patients at T_2 and T_3 were obvious increased than that of T_1 (P<0.05), the systolic pressure, diastolic pressure,and mean arterial pressure were significantly decreased (P<0.05). At T_1, T_2 and T_3 points, the heart rates of patients in the experimental group were greater than that of the control group (P<0.05). The systolic pressure, diastolic pressure, and mean arterial pressure had no dramatically differences between two groups (P>0.05). After AHH, the hematocrit and hemoglobin were obviously decreased in the experimental group at T_1, T_2, T_3 and T_4 points (P<0.05), with more blood platelets count and prolonged partial thromboplastin time (P<0.05). Compared to the control group, the Na~+, Cl~-was increased in the experimental group at T_1 and T_2 points (P<0.05), with greater hematocrit and hemoglobin at the T_5 and T_6 points (P<0.05). The results demonstrated that HSH can decrease or avoid transfusing blood in maxilla Lefort Ⅰ osteotomy.

Objective:To investigate the clinical efficacy and safety of Reduning injections in the treatment of infantile rotavirus enteritis. Methods:Totally 102 cases of infantile rotavirus enteritis in our hospital from June 2012 to June 2013 were randomly divided into two groups, the control group had 50 patients, and the treatment group had 52 cases. Both groups were given conventional symp-tomatic treatment, the control group was given 10 mg·kg-1 ribavirin injections in 100ml glucose injections for intravenous infusion, qd, and the treatment group was given 0. 5 ml·kg-1 Reduning injections in 100ml glucose injections for intravenous infusion, qd. Ev-ery 3-day was a course of treatment. The clinical curative effect of the two groups was observed, and the clinical symptoms, hospitaliza-tion time and adverse reactions were also studied. Results:After the treatment, the total effective rate in the control group was 70. 0%, while that in the treatment group was 92. 3%, and there was significant difference between the two groups (P<0. 05). The clinical symptom disappearance time and hospitalization time in the treatment group were significantly lower than those in the control group with statistically significant difference (P<0. 05). During the treatment, the control group had 5 cases of leucopenia and 2 cases of skin rash, and the patients all recovered after withdrawal of the drug, while the treatment group showed no obvious adverse reaction. Con-clusion:The curative effect of Reduning injections in the treatment of infantile rotavirus enteritis is promising, which can effectively improve the clinical symptoms, shorten the course of disease and reduce adverse reactions with the value of clinical application.

Abstract?AlM: To investigate the relationship between carotid artery stenosis and ischemic ocular diseases.?METHODS: The clinical data of 30 cases ( 37 eyes ) of patients with ischemic eye diseases were collected from November 2010 to May 2014, and they were accepted the fundus fluorescein angiography ( FFA ) , transcranial Doppler ( TCD) ultrasonic blood vessels of the eye, neck vascular color Doppler flow imaging ( CDFl) , the neck CT angiography ( CTA ) and carotid artery digital subtraction angiography ( DSA) examination, and then the ischemic eye disease patients with ocular symptoms were analyzed. The peak systolic velocity ( PSV) and resistance index ( Rl) of ophthalmic artery and central retinal artery were compared. Correlation between the internal carotid artery intima- media thickness ( lMT ) and ophthalmic artery, central retinal artery PSV and Rl correlation risk;ipsilateral internal carotid artery plaque and ophthalmic artery PSV and Rl; PSV and Rl associated ipsilateral internal carotid artery plaque and central retinal artery were analyzed.?RESULTS:Eye symptoms:a black dim, reduced vision, the eyes flash, and around the eye pain were 75. 7%, 83. 8%, 51. 4% and 32. 4%;The eye signs:the dilatation of retinal vein, retinal hemorrhage, arterial stenosis and cotton spot and the contralateral side were regarded as main signs. Ophthalmic artery PSV and Rl value of the differences were statistically significant ( P<0. 05 ); The contralateral side of the central retinal artery PSV and Rl value of the differences were statistically significant ( P<0. 05 ); The ipsilateral internal carotid artery lMT and ophthalmic artery, central retinal artery PSV and Rl were not correlated ( P>0. 05 ); The ipsilateral internal carotid artery plaque and ophthalmic artery PSV had no correlation with Rl values (P>0. 05); PSV and Rl and the ipsilateral internal carotid artery plaque and central retinal artery had no correlation (P>0. 05).?CONCLUSlON: The incidence of ischemic eye diseases and internal carotid artery stenosis is associated with very close, the clinical can regard the degree of internal carotid artery stenosis as an important basis for diagnosis and treatment of eye diseases.

Objective To determine multiple inorganic anions in drinking water with ion chromatography,such as F~-,Cl~-, NO_2~-,Br~-,HPO_4~(2-),NO_3~-,SO_4~(2-).Methods The ion chromatography was used.The optimized conditions were explored.Results Under the selected flow rate(1.5 ml/min),the optimal concentration of eluent NaHCO_3 and Na_2CO_3 was 2.0?10~(-3)and 3.0?10~(-3) mmol/L,under the concentration of eluent,the optimum pump flow rate was 1.5 ml/min,the recovery rate was 94.69%-102.20%. Conclusion This method is applicable to the determination of multiple inorganic anions in drinking water.

AIM:ToexplorewhetherMCP-1-JAK2/STAT3signaltransductioninthespinaldorsalhornin-volves the formation and development of rat type 2 diabetic neuropathic pain (DNP).METHODS: The male Sprague-Dawley rats were fed with a high-fat and fructose diet for 8 weeks,and then received a single intraperitoneal streptozocin in-jection to prepare the type 2 DNP model.The type 2 DNP rats were randomly divided into 4 groups (n=16):DNP group, MCP-1 neutralizing (DM) group, DNP+AG490 (DA) group and solvent control (SC) group.A catheter of PE-10 was placed into the subarachnoid space of the rats in groups DM , DA and SC.After 3 d, the rats in DM,DA and SC groups were injected with MCP-1 inhibitor 10μL at 0.1 mg/L, AG490 10μL at 1 mmol/L and DMSO 10μL at 3.5%once a day for 14 days, respectively.Another 16 normal rats were selected as control (C) group and were fed with common forage. The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were measured at 1, 3, 7 and 14 d after subarachnoid injection .The lumbar segments 4-6 of the spinal cord were removed at the same time for determination of the expressions of p-JAK2 and p-STAT3 by Western blot .RESULTS:Compared with C group , MWT was significantly de-creased, TWL was shortened and the expression of p-JAK2 and p-STAT3 in the spinal dorsal horn was up-regulated at 1, 3, 7 and 14 d in DNP and SC groups (P<0.05).Compared with DNP group, MWT was significantly increased, TWL was prolonged and the expression of p-JAK2 and p-STAT3 in spinal dorsal horn was down-regulated at 1, 3, 7 and 14 d in DM and DA groups (P<0.05).No significant difference in the MWT, TWL and expression of p-JAK2 and p-STAT3 between DNP group and SC group was observed (P>0.05).CONCLUSION:The MCP-1-JAK2/STAT3 signal transduction in the spinal dorsal horn involves the formation and development of DNP in rats .

Animals ; Carcinoma, Hepatocellular ; genetics ; virology ; Hepatitis B, Chronic ; complications ; Hepatitis C, Chronic ; complications ; Humans ; Liver Neoplasms ; genetics ; virology ; Neoplasm Recurrence, Local ; genetics ; Point Mutation ; Telomerase ; metabolism

Objective To investigate the effects of rosiglitazone on the endothelial regeneration and neointimal formation after balloon injury in rat aortic.Methods Endothelial denudation of aorta in SD rats were performed with 2 F Fogarty balloon catheter.Male Rats were randomly divided into the sham-operated group,the control group,and the RSG group.Aortic tissues were harvested 7 days and 14 days after the injury.The endothelial regeneration rate,serum NO level,IA/MA ratio and PCNA expression index were detected.Results RSG enhanced the reendothelialization of the injured aorta determined by Evans blue staining.The RA/TA ratio on the 7~(th) day and the 14~(th) day were 38.20% and 75.20%(P

Objective To evaluate the effect of low dose fentanyl on target plasma concentration (CP) of propofol (P) given by target controlled infusion (TCI) required to prevent response to proseal laryngeal mask insertion in 50% of patients (EC50). Methods Forty-six ASA Ⅰor Ⅱ patients aged 20-50 yr with body mass index ≤ 30 kg/m2 undergoing elective gynecological laparoscopy were randomly divided into 2 groups (n=23each):propofol group (P) and propofol-fentanyl group (PF). Anesthesia was induced with TCI of propofol. The initial Cp of propofol TCI was set at 2.5 μg/ml in group PF and 4 μg/ml in group P. Following equilibration between plasma and effect site concentration of propofol, fentanyl 1 μg/kg (in group PF) or normal saline (in group P) was injected iv. Laryngeal mask airway (LMA) was inserted at 3.5 min after fentanyl administration. EC50 was determined by up-and-down sequential trial. The ratio of Cp between the two consecutive patients was 1.2 in group P and 1.1 in group PF. LMA insertion response was defined as nausea, bucking and/or body movement during insertion. "Probit method was used to calculate the Echo and 95 % confidence interval required to prevent LMA insertion response. Results The EC50 and 95 % confidence interval of propofol given by TCI required to prevent LMA insertion response was 4.68 (4.20-5.21)μg/ml in group P and 2.63 (2.45-2.83) μg/ml in group PF. There was significant difference between the 2 groups. Conclusion Fentanyl 1 μg/kg iv can significantly reduce the Cp of propofol TCI required to prevent LMA insertion response.

BACKGROUND: Cartilage stent materials were abundant. With the development of preparation technique, structure, and surface modification technique, performance of materials were improved remarkably; thus, Integrated-repaidng defects of articular cartilage and subchondral bone required high stability of cartilage replacement materials.OBJECTIVE: To evaluate the effect of polyurethane/nano-hydroxyapatite+polyamide66 (PU/n-HA+PA66) biological composite material on repairing articular cartilage and subchondral bone.METHODS: Twenty dog models were randomly divided into 4 groups: porous PU/n-HA+PA66 implant group, compact PU/n-HA+PA66 implant group, n-HA+PA66 implant group and blank control group. The macroscopic and histological evaluations were performed at 4, 12, and 24 weeks postoperatively. The interfaces of biomaterial implants with articular cartilage were observed by scanning electron microscope.RESULTS AND CONCLUSION: After 12 and 24 weeks, bone tissue grew into lower layer materials. Porous PU was well fused with peripheral cartilage. At 24 weeks postoperatively, the porous PU was closely connected with surrounding articular cartilage than the compact PU, and the articular cartilage surrounding the compact PU degenerated obviously. PU/n-HA+PA66 had a similar structure with normal cartilage and subchondral bone and had remarkable effects on repairing defects of articular cartilage and subchondral bone.