In Trang An polyclinics in Ha Noi, with ELISA technique, 35 healthy people (27 female aged 20-38, 8 male aged 25-37) undergone an examination of serum CA153-3 and 31 other healthy people (17 female aged 21-38, 14 male aged 22-41) an examination of resum CA125. The technique is complicated needing precaution and accuracy; for each examination, it is recommended a standard graphic with a standard sample determined antigen concentration. CA15-3 normal concentration is 2.37-18.17 U/ml, and CA125 is 7.17-38.37 U/ml
Enzyme-Linked Immunosorbent Assay ; methods ; serum

Immunology plays an important role in prevention, discovery, diagnosis and treatment. There are 4 major immunological techniques including ELISA (enzyme linked immuno-sorbent assay), ICA (immuno-chromatography assay), GLORIA (gold labeled optical read rapid immunoassay) and MCIA (membrane capture immunoassay). These techniques with the simple test devices such as test paper, test stick, test cassette provided the rapid results. The immunological techniques have been applying in emergency tests (myocardial infection, embolism, thrombosis, and occlusion), infection viral infection, allergy, cancer and drug abuse
Immunology ; Immunoassay ; Enzyme-Linked Immunosorbent Assay

The markers of cancer were generated from cells of cancer or metabolism or immunoresponse to the canceration. For the last 2 decades of 20th century, many markers of cancer used clinically thanks to the development of new clone antibody. By developing the molecular biology. Many genes of cancer, telomere, telomerase, survivin were considered as markers of cancer have been detected for the recent 20 years.
neoplasms ; diagnosis

This paper introduced some differences of molecular biological techniques from microbiological techniques and some advantages of molecular biological techniques. They comprised fast and early detection, safety, convenient, effective, inexpensive techniques, allowance for diagnosis of causative agents that not able to culture, facilitate for prognosis and monitoring of the treatment. The molecular biological techniques showed its important role more and more and combined with microbiological technique to improve the diagnosis, treatment and prognosis
Communicable Diseases ; Molecular Biology

During transition years of 20th and 21st century, the molecular biology has had major progresses in the finding the nature of cancer's generation, early detection, prognosis and treatment of cancer. The molecular techniques were important breakthrough. The predominant achievement was to newly discover the role of telomere, telomerase and surviving in the cancer.
Molecular Biology ; Medical Oncology

This paper introduced some techniques of molecular biology in the diagnosis of some genetic diseases, pediatric diseases, as well as communicable and cardiovascular disease and cancer
Epidemiology, Molecular ; Pediatrics ; diagnosis

We have a step backwards in comparing with the previous 40 years in the education and the organization of medical laboratory examination. While our equipment and techniques have got new great success and the needs have increased. Typically, in clinical briefing, laboratory doctors and technicians have been always absent, it seems that it is due to the leak of a deep knowledge and a large skill of laboratory. Therefore their roles were reduced. An improvement in the programm of formation of laboratory doctors and experts with various branches: biochemia, hematology, blood perfusion was recommend
Outpatient Clinics, Hospital ; Technology, Medical ; Laboratories ; diagnosis ;

Disease produce cancer is quite complicated. Cancer arise and develope by the disorder of growth control function and disorder of sucidal cells process, the forming artery, metastasis and a sudden mutation of gene. Mainly factors that produce cancer are caused by genetic factor, life style and enviromental factors. These ones influence mutually. Currently, diagnosis of cancer are often late. Some tumor markers are used for prediction of cancer. Some evidences on early detective value of metastasis cancer in some cancer gene tests in tissue and blood by using RT-PCR
Molecular Biology ; Neoplasms ; Tumor Markers, Biological

The great progresses of molecular biology also contribute to find out basic multisided of disease formed cancer. The main marks of molecular tumor are: pregene tumor, gene tumor, gene-inhibition tumor, telemerase, and survivin, acid nucleic. Molecular tumor are based for some new therapy methods to cure cancer: gene therapy, immune therapy, prevent of tumor growing up and penetrate tumor cells
Molecular Biology ; Neoplasms ; Neoplasms, Germ Cell and Embryonal

The development of molecular biology help to clearly understand about pathophysiological mechanisms of diabetes mellitus are due to genetic factors and life environment. The diabetes divided up: type 1 diabetes, type 2 diabetes, and other types. In 1999, American Diabetes Association and WHO advanced a new classification, following: type A (type 1 diabetes), type B (type 2 diabetes), type C (MODY - onset in young adult), type D (diabetes at pregnancy and tolerated glucose disorders in pregnancy)
Diabetes Mellitus ; Diabetes Complications ; Molecular Biology ; Knowledge