1.Comparison of anapathological and clinical features in 3 cases of benign parathyroid adenoma
Journal of Vietnamese Medicine 2002;277(10):50-55
The authors report the clinical and pathological study of three cases of parathyroid adenoma diagnosed and surgically treated at ViÖt §øc hospital during 3 years (2000-2002). The clinical and paraclinical symptoms were typical. One case showed symptoms of bone disease with the features of classic osteitis fibrosa cystica or Von Reckling Hausen disease. One patient had clinical features of peptic ulcer and one case had compressive symptoms of neck mass. Two patients had a features of too high and continuous hypercacimia (>3.2 mmol/l). The third case had normally laboratory findings. All tumors had typical pathological features of parathyroid adenoma. Two cases had been diagnosed and treated late and one patient was diagnosed as thyroid cyst. The clinical and paraclinical symptoms were discussed
Parathyroid Neoplasms
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Pathological Conditions, Signs and Symptoms
2.A study of pathological characteristics and BRAF V600E status in Langerhans cell histiocytosis of Vietnamese children
Thu Dang Anh PHAN ; Bao Gia PHUNG ; Tu Thanh DUONG ; Vu Anh HOANG ; Dat Quoc NGO ; Nguyen Dinh The TRINH ; Tung Thanh TRAN
Journal of Pathology and Translational Medicine 2021;55(2):112-117
Background:
Langerhans cell histiocytosis (LCH) is more common in children than adults and involves many organs. In children, the BRAF V600E mutation is associated with recurrent and high-risk LCH.
Methods:
We collected paraffin blocks of 94 pediatric LCH patients to detect BRAF V600E mutation by sequencing. The relationship between BRAF V600E status and clinicopathological parameters were also critically analyzed.
Results:
BRAF V600E mutation exon 15 was detected in 45 cases (47.9%). Multiple systems LCH showed a significantly higher BRAF V600E mutation rate than a single system (p=.001). No statistical significance was evident for other clinical characteristics such as age, sex, location, risk organs involvement, and CD1a expression.
Conclusions
In Vietnamese LCH children, the proportion of BRAF V600E mutational status was relatively high and related to multiple systems.
3. 20-Hydroxyecdysone from Dacrycarpus imbricatus bark inhibits the proliferation of acute myeloid leukemia cells
Trinh Thi THUY ; Nguyen Thanh TAM ; Nguyen Thi Hoang ANH ; Dang Viet HAU ; Tran VAN SUNG ; Dinh Thi PHONG ; Le Quoc THANG ; Sabrina ADORISIO ; Domenico V. DELFINO
Asian Pacific Journal of Tropical Medicine 2017;10(2):157-159
Objective To investigate the anti-proliferative effects of 20-hydroxyecdysone isolated from the bark of Dacrycarpus imbricatus (Blume) de Laub. Methods Column chromatography was used for isolation of compounds from plant material. The structure of the isolated compound was identified by mass spectrometry and nuclear magnetic resonance techniques, including HSQC, HMBC, NOE-difference experiments. The isolated compound was tested for its anti-proliferative activity in acute myeloid leukemia (AML) and OCI-AML cells. Results Compound 1 was isolated from the ethyl acetate fraction of Dacrycarpus imbricatus barks by column chromatography. Its chemical structure was identified as 20-hydroxyecdysone (20HE), a cholestane-type ecdysteroid, by a combination of mass spectrometry and nuclear magnetic resonance spectrometric analyses. Our goal was to test the anti-proliferative activity of 20HE using the OCI-AML cell line. 20HE significantly decreased OCI cell number at a concentration of 1 mg/mL, whereas lower concentrations were ineffective. Moreover, this decrease was due to partial blockage of the G