Background: Acute Renal Failure (ARF) is a common complication in severe traumatic patients. It can cause higher mortality in these patients. Clear knowledge about the risk factors for acute renal failure that can limit this complication. Objective: To estimate some risk factors for acute renal failure in severe traumatic patients. Subjects: The study carried out on 125 severe traumatic patients who were treated in ICO at Viet Duc hospital. Method: It is a prospective cohort study. Diagnosis of ARF was based on blood creatinine level. Blood creatinine >130 micromol/l was considered ARF. Results: There were 49 severe traumatic patients with ARF (39.2%). The risk factors of univariable for ARF were included: ISS > 32 (OR = 3.80), retroperitoneal hematoma (OR = 3.51), high dose of catecholamine, severe infection after trauma (OR = 10.08), respiratory failure (OR = 6.38), intra-abdominal bleeding (OR = 2.80), hypotension over one hour (OR = 6.46) and rhadomyolysis (OR = 4.62). With multivariable analysis, it was discovered that rhadomyolysis (OR = 4.11), hypotension over one hour (OR = 3.74) and severe infection after trauma (OR = 2.38) were risk factors for ARF. Conclusion: Rhadomyolysis, hypotension over one hour and severe infection after trauma was a risk factors for ARF in severe traumatic patients.
Severe traumatic patient ; risk factors ; acute renal failure

Background: Deletion and duplication mutations of dystrophin gene make up from 70 to 75% of patients with Duchenne Muscular Dystrophy (DMD). Two thirds of children with DMD inherited from the heterozygous mothers the mutated gene which is located on one of the sex chromosomes. Objective: To detect the asymptomatic carriers of dystrophin gene mutation using molecular techniques. Subject and methods: 3 DMD patients and their 9 relatives. Using techniques: DNA extraction and quantitative Polymerase Chain Reaction (PCR). Results: Successfully detected 4 heterozygous individuals from 9 female members of three different families that have already confirmed DMD patients. Conclusion: This method could lead to a new way of prenatal diagnosis of DMD as well as other genetic disorders that are caused by deletion or duplication mutation.
Duchenne muscular dystrophy ; carrier ; quantitative PCR

Background: Noise pollution has seriously negative effects on worker\u2019s health, especially their hearing ability. Occupational hearing loss is one of 21 insured occupational diseases. Occupational hearing loss is common in workers in transpostation, construction, energy heavy and light industry areas. Objectives: 1. To determine the degree of noise pollution in Thua Thien Hue Unity Auto Mechanical Joint Stock Company. 2. To evaluate occupational failing hearing and occupational hearing loss among workers in the company. 3. To promote preventive measures which imnimize occupational failing hearing and occupational hearing loss. Subjects and method:A cross-sectional, descriptive study was carried out in 254 employees in Thua Thien Hue Unity Auto Mechanical \r\n', u'Joint Stock Company. Results and Conclusion: 72.7% the employees of Thua Thien Hue Unity Auto Mechanical \r\n', u'Joint Stock Company have exposed to environmental noise at the level higher than the permitted one. The rate of occupational failing hearing among these people is rather high (33.1%), and occupational hearing loss rate is 11.8%. There is a increased rate of occupational hearing loss according to the employee\u2019s age and seniority. Proposed preventive measures should be immediately conducted (P < 0.05).\r\n', u'
Hearing Loss ; Noise-Induced/ epidemiology ; etiology ; pathology ;

Background: In Viet Nam, there were many researches on determining lead level in blood, food and environment. But until now, there were not researches on determining lead level in fishermen community. Objectives: Determine trace amount of lead by von-ampe dissolve anot using electrode BiFE. Analysis on trace amount of lead in blood and urine of fishermen in Canh Duong village. Propose solutions for preventing lead poisoning in studied community. Subjects and method: Fishermen in Canh Duong village, Phu Loc, Thua Thien Hue province. The method differential pulse-anodic stripping voltametry (DP-ASV) using Bismuth Film Electrodic in situ was successfully applied to measure lead levels in blood and 24h urine of fishermen in Canh Duong village. Results: The mean level of lead in blood of the suspected group (fishermen) in Canh Duong village (34,7 \xb5g/dL) was higher than that of the control group (16,3 \xb5g/dL). For both groups, 37 out of 40 blood samples (92,5%) had lead level higher than WHO\u2019s recommended level (10 \xb5g/dL). Especially, 6 of 40 blood samples (15%) had lead level higher than 50 \xb5g/dL. The lead level in 24h urine of suspected group had slightly higher (about 1,2 times) than that in the control group. The lead level of the two groups was much higher than the normal level. Conclusion: The method DP-ASV/BiFE in situ was applied successfully to determine lead level in blood and 24h urine. The investigative results of related information showed that: the risk of chronic lead poisoning in studied community very high.
Lead/ adverse effects ; chemistry ; antagonists & ; inhibitors ;

Background: Fragile X Syndrome (FXS) is the most common cause of inherited mental retardation. The absence of Fragile X Mental Retardation (FMRP) in Fragile X syndrome changes other proteins. Objective: To detect changes of glycoprotein in human serum of Fragile X syndrome. Subject and methods: Affinity chromatography with lectin concanavalin A (ConA) used to receive glycoprotein. The collected glycoprotein was then separated using 2-D electrophoresis. The protein spots were further excised, trypsin digested, and analyzed by nano LC couple with ESI-MS/MS and identified by MASCOT v1.8 software. Results and conclusion: 5 glycoproteins showed the different expression levels in the serum of Fragile X syndrome. Haptoglobin, Ig-J were increased and ceruloplasmin, transferring, Ig kappa were decreased. Using affinity chromatography with lectin concanavalin A (ConA), glycoprotein was received and divided on 2 ways electrokinetic chromatography. The mixture protein was identified with a reliability of 99.5% by 2 ways liquid chromatography combined with continuous spectrum mass.
Fragile X syndrome ; Fragile X Mental Retardation ; proteomics

Objectives: The prevalence of posttraumatic stress disorder (PTSD) has increased, particularly among individuals who have recovered from coronavirus disease 2019 (COVID-19) infection. Health literacy is considered a “social vaccine” that helps people respond effectively to the pandemic. We aimed to investigate the association between long COVID-19 and PTSD, and to examine the modifying role of health literacy in this association. Methods: A cross-sectional study was conducted at 18 hospitals and health centers in Vietnamfrom December 2021 to October 2022. We recruited 4,463 individuals who had recovered from COVID-19 infection for at least 4 weeks. Participants provided information about their sociodemographics, clinical parameters, health-related behaviors, health literacy (usingthe 12-item short-form health literacy scale), long COVID-19 symptoms and PTSD (Impact Event Scale-Revised score of 33 or higher). Logistic regression models were used to examine associations and interactions. Results: Out of the study sample, 55.9% had long COVID-19 symptoms, and 49.6% had PTSD.Individuals with long COVID-19 symptoms had a higher likelihood of PTSD (odds ratio [OR], 1.86; 95% confidence interval [CI], 1.63–2.12; p < 0.001). Higher health literacy was associated with a lower likelihood of PTSD (OR, 0.98; 95% CI, 0.97–0.99; p = 0.001). Compared to those without long COVID-19 symptoms and the lowest health literacy score, those with long COVID-19 symptoms and a 1-point health literacy increment had a 3% lower likelihood of PTSD (OR, 0.97; 95% CI, 0.96–0.99; p = 0.001). Conclusion Health literacy was found to be a protective factor against PTSD and modified the negative impact of long COVID-19 symptoms on PTSD.