The awareness on tuberculosis was determined by an interview conducted on 559 subjects with a symptomatic of cough persisting for > 3 weeks (259 male and 300 female subjects of > 15 year old age). Results showed an insufficiency of knowledge, especially in female subjects. Mass media play an effective role in health education but only > 50% of population could access them frequently
Tuberculosis ; Cough ; Disease ; Awareness ; epidemiology

Background: Tuberculosis (TB) is still one of the diseases with highest incidence and mortality world-wide. Vietnam ranked 13 of 22 countries with highest number of tuberculosis patients in the world. Methods and channels is one factor among some important factors which affect effectiveness of health communication activities in general and communication activities for prevention of tuberculosis in particular. Objectives: To describe probable for access tools of health communication among general inhabitants and to determine which methods and tools should be used. Subjects and method: This was a cross-sectional described study. A population based survey was carried out within a demographic surveillance site in Bavi, Ha Tay province. Subjects of the study were adults (> 15 years old) randomly identified at households level. Results and Conclusion: A total of 12143 persons were interviewed. People received TB information from many sources. Commonly, reported sources of information included television (64.6%) and friends/relatives (42.7%). There were different sources of information between men and women. The use of more than one method/channel should be considered in TB health education activities. Commonly, television and loudspeaker were suggested (70.4% and 55.1%).
Tuberculosis/ prevention & ; control ; Residence Characteristics ; Health Communication ;

Background: In Vietnam, although there were interventions in perinatal and neonatal care services, the neonatal mortality reduced slowly, accounting for >50% of under-5-year-old mortality rate and >70% of under-1-year-old mortality rate. \r\n', u'Objectives: Discover the status of prenatal, perinatal, and postnatal care services, and analyze the relationships between healthcare services and neonatal mortality in Quang Ninh province. Subjects and method: Information on births and neonatal deaths between January and December 2005 in Quang Ninh province was collected. The perinatal care services including antenatal, delivery and post partum cares were described. The relationship between neonatal deaths and places of delivery was analyzed in order to uncover the risk of death in newborns delivered at home. Results: 76% of pregnant women received 3 or more antenatal care visits. More than 90% of deliveries took place at health facilities, of them, 16% occurred in community health stations. Only 49% of mothers received post partum care visits, with 82% of them received the visits in the first week after delivery. There were 17,519 births and 284 neonatal deaths in 2005. The neonatal mortality rate was lowest in cases born in community health stations (7.5/1.000) and highest in home birth cases (39/1.000). Conclusion: Further improvement in safe motherhood and neonatal survival can be achieved by increasing the accessibility of women to perinatal cares during pregnancy, delivery. \r\n', u'
perinatal care services ; neonatal mortality

Background: Pediatric emergency at the inter-hospital transfer lines is a serious problem in the care and treatment of children. 24 hours mortality after admission rose higher than previously.\r\n', u'Objectives: The aim of the study was to determine the 24 hours mortality and to analyze the causes of 24 hours mortality.\r\n', u'Subjects and methods: The study was carried out on 189 patients, who died during 24 hours after admission at Nghe An Pediatric hospital from July 2002 to June 2004.\r\n', u'Results: The results showed that the 24 hour mortality consisted of 77% of all deaths in the hospital. The neonatal mortality rate among the admitted patients was the highest. The most common causes of deaths were: respiratory and neurological diseases with 48% of the total (24% each) followed by injury (15%). Asphyxia/premature ranked third (9%). Surgical diseases consisted of 8.5% and infectious diseases contributed 6% of all deaths. \r\n', u'Conclusion: The interventions at hospital should prioritize the improvement of knowledge and skills on neurological, respiratory, trauma and surgical emergency care. The urgent need is also to train staff and to supply equipment for neonatal care. \r\n', u'
Mortality ; children

Background: In recent years, Vietnam has applied four methods (morphology, cell chemistry, immune marker classification, cyto genetic) in diagnosis and used multi-chemotherapy in treatment for acute myelogenous leukemia (AML)\r\n', u'Objectives: To initially determine some fusion gene transcripts in the acute myelogenous leukemia patients by applying PCR technique. Subject and method: The study included 19 patients with acute myelogenous leukemia treated in National Institute of Hematology and Blood Transfusion and Bachmai Hospital from April 2007 to August 2007. RNA were extracted from leukemic cells and PCR for AML1/ETO, CBFP/MYH11, PMR/RARa fusion transcript was done. Results: Number of male patients was 6 (32%), female patients was 13 (68%). The average age of these patients was 32.67 \xb113.62. There were three M4, M4eo patients with AML1/ETO gene (accounting for 16%), two M2, M4 patients with CBF/MYH1 gene and type F of genetic modification accounting for 11%), two M3 patients with PMR/RAR\u03b1 and Bcr3 of genetic modification (accounting for 11%). Conclusion: Results of the study did not differ significantly from other researches in the world. This study showed the need of applying the PCR technique in determining fusion gene transcript together with traditional cyto-genetic method.\r\n', u'\r\n', u'
Leukemia ; Myeloid ; Acute/ blood ; pathology ; complications ; Polymerase Chain Reaction

Background: Patients who received multiple transfusions of blood and blood products may produce antibodies against antigens of erythrocytes, leukocytes, platelets etc, resulting in many clinical implications. Objectives: To detect frequencies of antineutrophil antibodies in multitransfused patients at National Institute of Hematology and Blood Transfusion (NIHBT). Subjects and methods: The study was conducted on 30 multitransfused patients. Among them there were 12 with thrombocytopenia and 18 with aplastic anemia. Results: 6 cases had anti - neutrophil antibodies, of which 5 had more than 5 times of transfusion, 4 with aplastic anemia and 2 with thrombocytopenia. The sera were further tested with neutrophil panel, revealing 4 samples with anti - NA 1 (13.3%) and 1 sample with anti - NA2 (3.3%). The frequency of anti - neutrophil antibodies in multitransfused patients at IHBT in the study is 20%. Conclusion: Frequency of anti-NA1 was higher than anti-NA2 in multitransfused patients at NIHBT and directly proportional by frequency of NA1 and NA2 antigens in this group. The technical process to identify and classify antineutrophil antibodies in this study can be applied for patients who received multiple transfusions of blood and blood products in Viet Nam
Anemia ; Aplastic/ blood ; complications ; pathology ; Neutrophils

Background: In Vietnam, there are a number of studies on the application of ATRA in treating acute promyelocytic leukemia (AML \u2013 M3) but they have still faced with certain difficulties. Objectives: (1). Study PML/RAR alpha fusion gene on the patients diagnosed with AML \u2013 M3. (2). Study the index of hematology of the PML/RAR alpha positive group. Subject and Method: 21 patients with acute promyelocytic leukemia (M3) were studied. All patients were examined with morphology, coagulation and cytogenetic tests and RNA were extracted from leukemic cells and PCR for PML/RAR alpha fusion transcript. Result and conclusion: PML/RAR alpha positive in 67% including 4 patients which were not discovered t(15; 17) by cytogenetic technique. Rates of three subtype (bcr1, bcr2 and bcr3) of PML/RAR alpha were 7 patients (50%), 3 patients (21,5%) and 4 patients (28,5%), respectively. WBC and bone marrow cells of PML/RAR alpha positive group were 5.08+/-3.87 and 155.82+/-106.21. D \u2013 Dimer level was 1954.89+/-1575.28; 93% of patients in the PML/RAR alpha positive group had DIC.
Acute promyelocytic leukemia ; M3 ; PML/RAR alpha

Background: Chromosome mutation type t(8;21) has quite a high frequency in acute myelogenous leukemia, which accounted for about 15% among adult patients. From 2001, the WHO has a new classification for acute myelogenous leukemia based on genetic mutations. Form had AML1/ETO were arranged into genetic mutation group with better prognosis and ability to fully recover after chemotherapy with a high dose of cytarabin. Objective: Study AML1/ETO fusion gene on the patients diagnosed with Acute Myelogenous Leukemia (AML), as well as the clinical features and some haematologic parameters of the AML1/ETO positive group. Subject and methods: 76 patients with AML were treating in the National Institute of Hematology & Blood Transfusion and the Department of Hematology & Blood Transfusion of Bach Mai Hospital from April 2007 to July 2008. These patients were studied for clinical examination, morphology and RNA were extracted from leukemic cells and PCR for AML1/ETO fusion transcript was performed. Results and conclusions: The incidence of AML1/ETO positive in the AML patients was 24%. The incidence of AML1/ETO positive in AML-M2 was 28%. In the AML1/ETO positive group: median age was 26.94+/-9.22; rate of severe anemia, hemorrhage, fever, infection, hepatomegaly, splenomegaly, lymphadenopathy and gum hypertrophy was 44%, 33%, 28%, 11%, 44%, 28%, 17% and 6%, respectively. Median hemoglobin, WBC, platelet, bone marrow cell count, % blast in peripheral blood and in bone marrow was 84.41+/-28.97 g/l, 29.42+/-31.36 g/l, 42.12+/-33.83 g/l, 215.93+/-134.42 g/l, 56.21+/-26.58% and 65.14+/-16.12%, respectively.
acute myelogenous leukemia ; AML1/ETO fusion gene

Background: In Vietnam, the HIV/AIDS pandemic is quickly spreading, almost totally in young people and among injecting drug users.The transition phase from smoking to injecting is very important for the life of injecting drug users because it is a risk factor of disease transmission. Objectives: (1) To analyze the transition phase from smoking/sniffing to injecting of the young male heroin users in Ha Noi. (2) To describe some factors related to the transition phase in these people. Subjects and method: A cross-sectional study carried out from September 2004 to September 2005 at 9 districts in Ha Noi city. The subjects were 1115 young men aged 16 to 29 years, who has been living in Ha Noi for over 6 months and smoked/sniffed heroin within the last 30 days. Results and conclusions: The average time of drug user in the 1115 subjects was 4.7 years. The majority started using drug at the age of 18.4 +/- 3.1 years. In which, 71 % consume the drugs by injection. The average time from smoking to injecting is 2.49 +/- 2.2 years. The main reasons for injecting drugs were for being faster \u2018on the height\u2019 (42%) and cheaper (40%). Some factors related to the transition phase included: age of the first heroin use, year of the first heroin use, situation for migrating into Ha Noi, knowledge level, the kind of drug used for the first time. Young people began using heroin in the year 2000 had a quicker transition phase than those using heroin before 1997 (p< 0.01).
injecting drug user ; drug user ; heroin

Background: Recently, microplate almar blue assay has been used commonly in detecting gen related to tuberculosis drug resistance, which provides results after 5-7 days with lower cost compared to traditional methods. Objective: To evaluate the application of microplate almar blue assay for rapid detection of drug resistance of M. tuberculosis strains. Subject and Method: A microplate-based assay which uses Alamar blue reagent - an oxidation reduction dye (MABA), was used for the determination of the anti-tuberculosis drug (isoniazid-INH, rifampicine-RIF, streptomycine-STR and ethabuton=EMB) resistance of 96 M. tuberculosis strains isolated from Vietnamese patients in comparison to those obtained by conversional method. Result: MABA showed to have high sensitivity and specificity in testing the sensitivity to individual anti-tuberculosis drugs (from 82.4% for STR to 93.3% for - INH and from 82.5% for EMB to 98.4% for STR; respectively), as well as for the multi-drug resistant M.tuberculosis (86.4% of sensitivity), highly correlated with the result determined by proportion method. Conclusion: MABA reveals the advantage in shortening test time, in simple performance and lower cost compared with the conversional culture based methods.
Microplate almar blue assay ; M. tuberculosis ; drug resistance