The live and cryoprecipitate BCG had been produced successfully at Nha Trang - Da Lat Institute of vaccine and biological substances (Im. BCG) of 37.5 mg/vial met standard of Ha Noi National Center for Vaccine and Biological substance control and WHO. Im.BCG met the standards of the sterillization and the safety. The live unit attained 2.6 +/- 0.54 x 108 per vial, the rate of thermal stability was 58.2 +/- 0.023, the dispension density was 1.22 +/- 0.119 and the additive huminity was 2.46 +/- 0.27%.
Mycobacterium bovis ; vaccines

The live and cryoprecipitate BCG had been produced successfully at Nha Trang - Da Lat Institute of vaccine and biological substances (Im. BCG) of 37.5 mg/vial met standard of Ha Noi National Center for Vaccine and Biological substance control and WHO. Im.BCG met the standards of the sterillization and the safety. The live unit attained 2.6 +/- 0.54 x 108 per vial, the rate of thermal stability was 58.2 +/- 0.023, the dispension density was 1.22 +/- 0.119 and the additive huminity was 2.46 +/- 0.27%.
Mycobacterium bovis ; vaccines

Nha Trang National Institute of Vaccine had produced successfully BCG vaccine of local norm standard (5mg/ampulla) met the criterion issued by Hanoi National Centre of biopreparation quality control. Living units: 3.33  0.51 x 106 units; rate of thermo-stability 78.8  4.7%; photo density 0.23  0.018; dispersive density 1.11  0.075 and residual humidity 215  0.21%
BCG Vaccine ; Vaccines ; immunization

The aim of this study is to delineate ‘admixed hybrid’ and ‘introgressive’ Fasciola genotypes present in the Fasciola population in Vietnam. Adult liver flukes collected from ruminants in 18 Provinces were morphologically sorted out by naked eyes for small (S), medium (M) and large (L) body shapes; and human samples (n=14) from patients. Nuclear ribosomal (rDNA) ITS1 and ITS2, and mitochondrial (mtDNA) nad1 markers were used for determination of their genetic status. Total 4,725 worm samples of ruminants were tentatively classified by their size: 6% (n=284) small (S)-, 13% (n=614) medium (M)-, and 81% (n=3,827) large (L)-forms. All the representative (n=120, as 40 each group) and 14 human specimens, possessed maternal mtDNA of only F. gigantica and none of F. hepatica. Paternally, all (100%) of the L-(n=40) and 77.5% (n=31) of the M-flukes had single F. gigantica rDNA indicating ‘pure’ F. gigantica. A majority (90%, n=36) of the S- and 15% (n=6) of the M-worms had single F. hepatica rDNA, indicating their introgressive; the rest (10%, n=4) of the S- and 7.5% (n=3) of the M-flukes had mixture of both F. gigantica and F. hepatica rDNAs, confirming their admixed hybrid genetic status. Fourteen human samples revealed 9 (64%) of pure F. gigantica, 3 (22%) of introgressive and 2 (14%) of admixed hybrid Fasciola spp. By the present study, it was confirmed that the small worms, which are morphologically identical with F. hepatica, are admixed and/or introgressive hybrids of Fasciola spp., and able to be the pathogens of human fascioliasis.
Adult ; DNA, Mitochondrial ; DNA, Ribosomal ; Fasciola hepatica ; Fasciola ; Fascioliasis ; Genotype ; Humans ; Liver ; Ranunculaceae ; Ruminants ; Vietnam