We report a case of dedifferentiated liposarcoma of retroperitoneum as a recurrent form in a 41 year old male. The patient received a extirpation for retroperitoneal mass and diagnosed as myxoid liosarcoma 4 years ago. The patient experienced 3 recurrences over a period of 4 years and diagnosed as myxoid liposarcoma in the second, third recurrence also. Histologically, the mass was composed of several clearly distinct elements : well differentiated liposarcoma, myxoid liposarcoma, myxoid malignant fibrous histiocytoma, poorly differntiated sarcoma, and fibrosarcoma. Immunohistochemically, S-100 protein was expressed in the area of spindle cell sarcoma, well differentiated liposarcoma, and malignant fibrous histiocytoma but alpha-1-antichymotrypsin was only expressed in the area of myxoid malignant fibrous histiocytoma.

It has been known that activation of tyrosine kinases is involved in signal transduction. Role of the tyrosine kinase in vascular smooth muscle contraction was examined in deoxycorticosterone acetate (DOCA)-salt hypertensive rats. Male Sprague-Dawley rats underwent uninephrectomy, one week after which they were subcutaneously implanted with DOCA (200 mg/kg) and supplied with 1% NaCl and 0.2% KCl drinking water for 4-6 weeks. Control rats were treated the same except for that no DOCA was implanted. Helical strips of carotid arteries were mounted in organ baths for measurement of isometric force development. Genistein was used as a tyrosine kinase inhibitor. Concentration-response curves to 5-hydroxytryptamine (5-HT) shifted to the right by genistein in both DOCA-salt hypertensive and control rats. Although the sensitivity to genistein was similar between the two groups, the maximum force generation by 5-HT was less inhibited by genistein in arteries from DOCA-salt hypertensive rats than in those from controls. Genistein-induced relaxations were attenuated in arteries from DOCA-salt rats. Genistein affected the contraction to phorbol 12, 13-dibutyrate (PDBu) neither in DOCA-salt nor in control arteries. These observations suggest that tyrosine kinase is involved in 5-HT-induced vascular contraction, of which role is reduced in DOCA-salt hypertension.
Animals ; Arteries ; Baths ; Carotid Arteries ; Desoxycorticosterone Acetate ; Desoxycorticosterone* ; Drinking Water ; Genistein ; Humans ; Hypertension ; Male ; Muscle, Smooth, Vascular ; Phosphotransferases* ; Protein-Tyrosine Kinases ; Rats* ; Rats, Sprague-Dawley ; Relaxation ; Serotonin ; Signal Transduction ; Tyrosine*

Although many studies show that thromboxane A2 (TXA2) has the action of gastrointestinal (GI) motility using GI muscle cells and tissue, there are no reports on the effects of TXA2 on interstitial cells of Cajal (ICC) that function as pacemaker cells in GI tract. So, we studied the modulation of pacemaker activities by TXA2 in ICC with whole cell patch-clamp technique. Externally applied TXA2 (5 micrometer) produced membrane depolarization in current-clamp mode and increased tonic inward pacemaker currents in voltage-clamp mode. The tonic inward currents by TXA2 were inhibited by intracellular application of GDP-beta-S. The pretreatment of ICC with Ca2+ free solution and thapsigargin, a Ca2+-ATPase inhibitor in endoplasmic reticulum, abolished the generation of pacemaker currents and suppressed the TXA2-induced tonic inward currents. However, chelerythrine or calphostin C, protein kinase C inhibitors, did not block the TXA2-induced effects on pacemaker currents. These results suggest that TXA2 can regulate intestinal motility through the modulation of ICC pacemaker activities. This modulation of pacemaker activities by TXA2 may occur by the activation of G protein and PKC independent pathway via extra and intracellular Ca2+ modulation.
Animals ; Benzophenanthridines ; Endoplasmic Reticulum ; Gastrointestinal Motility ; Gastrointestinal Tract ; GTP-Binding Proteins ; Guanosine Diphosphate ; Interstitial Cells of Cajal ; Intestines ; Membranes ; Mice ; Muscle Cells ; Naphthalenes ; Patch-Clamp Techniques ; Protein Kinase C ; Thapsigargin ; Thionucleotides ; Thromboxane A2

BACKGROUND: An increase of the extracellular K+concentrations up to about 8 mM in the isolated vessels causes relaxation in pre-contracted state. In order to elucidate the mechanisms of K+induced relaxation and compare with that of 2-kidney, 1 clip (2K1C) renal hypertensive rats, we recorded aortic vascular tension using an organ bath study. METHOD: 2K1C hypertension was made by clipping the left renal artery and age-matched control rats received a sham treatment. Thoracic aortic rings were mounted in tissue baths for measurement of isometric contractile force. RESULTS: Exposure to K+(from 2 to 8 mM) relaxed a phenylephrine (2 x 10-6 M)-induced contraction in K+free Krebs-Ringer solution, dose-dependently. Ouabain (10-5 M) enhanced the K+induced relaxation in above 2 mM K+ The K+induced relaxation was still induced in endothelium-denuded condition. Incubation with the K+channel blockers such as tetraethylammonium (TEA, 1 mM), glibenclamide (10-5 M), 4-aminopyridine (3 mM), barium (5 mM) and cesium (2 mM) did not affect on the K+induced relaxation. In renal hypertensive rats, the K+induced relaxation was markedly suppressed and ouabain enhanced it. CONCLUSIONS: These results suggest that the K+induced relaxation in aorta be mediated by Na-pump independent mechanisms, and the decrease of the K+induced relaxation in the renal hypertensive rats may be a possible mechanism of hypertension.
4-Aminopyridine ; Animals ; Aorta ; Barium ; Baths ; Cesium ; Glyburide ; Hypertension ; Muscle, Smooth* ; Ouabain ; Phenylephrine ; Placebos ; Rats* ; Relaxation ; Renal Artery ; Tetraethylammonium ; Vasodilation*

BACKGROUND: An increase of the extracellular K+concentrations up to about 8 mM in the isolated vessels causes relaxation in pre-contracted state. In order to elucidate the mechanisms of K+induced relaxation and compare with that of 2-kidney, 1 clip (2K1C) renal hypertensive rats, we recorded aortic vascular tension using an organ bath study. METHOD: 2K1C hypertension was made by clipping the left renal artery and age-matched control rats received a sham treatment. Thoracic aortic rings were mounted in tissue baths for measurement of isometric contractile force. RESULTS: Exposure to K+(from 2 to 8 mM) relaxed a phenylephrine (2 x 10-6 M)-induced contraction in K+free Krebs-Ringer solution, dose-dependently. Ouabain (10-5 M) enhanced the K+induced relaxation in above 2 mM K+ The K+induced relaxation was still induced in endothelium-denuded condition. Incubation with the K+channel blockers such as tetraethylammonium (TEA, 1 mM), glibenclamide (10-5 M), 4-aminopyridine (3 mM), barium (5 mM) and cesium (2 mM) did not affect on the K+induced relaxation. In renal hypertensive rats, the K+induced relaxation was markedly suppressed and ouabain enhanced it. CONCLUSIONS: These results suggest that the K+induced relaxation in aorta be mediated by Na-pump independent mechanisms, and the decrease of the K+induced relaxation in the renal hypertensive rats may be a possible mechanism of hypertension.
4-Aminopyridine ; Animals ; Aorta ; Barium ; Baths ; Cesium ; Glyburide ; Hypertension ; Muscle, Smooth* ; Ouabain ; Phenylephrine ; Placebos ; Rats* ; Relaxation ; Renal Artery ; Tetraethylammonium ; Vasodilation*

BACKGROUND: Calcium plays a key role in vascular contraction and regulates receptor sensitivity to certain neurotransmitters. Calcium channel blockers are useful in the treatment of both clinical and experimental hypertension. The present study was designed to examine whether there is an alteration of the activity of calcium channels in association with the development of hypertension. METHODS: Deoxycorticosterone acetate(DOCA)-salt hypertension was made by subcutaneous implantation of DOCA(200mg/kg)strip plus saline drinking(1%) and 2-kidney, 1 clip(2KIC)hypertension by clipping the left renal artery with a silver clip(internal gap of 0.2mm). They were used 4 weeks later. Age-matched normal rats served as a control. Mean arterial pressure(MAP) and heart rate(HR) were continuously recorded from the right femoral artery. The drugs were administered intravenously. RESULTS: Vehicle alone was without effect on MAP or HR. In normotensive rats, nifedipine infusion(5 and 10ug/kg/min)caused a dose-dependent decrease in MAP without significant changes in HR, while Bay k 8644(Bay K, 5 and 10 ug/kg/min) increased MAP transiently. Both the depressor response to nifedipine and the pressor response to Bay k were more marked in DOCA-salt hypetensive rats than in normotensive rats. The maximal changes in MAP indced by nifedipine(5 and 50 ug/kg) or Bay K(5 and 50 ug/kg) were also enhanced in 2KIC hypertensive rats as compared with control rats. CONCLUSION: These results indicate that calcium channel inhibitors and activators can affect on the regulation of blood pressure in an opposite fashion. It is also suggested that the activity of calcium channels might be altered in the developement of experimental hypertension.
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester* ; Animals ; Bays* ; Blood Pressure ; Calcium ; Calcium Channel Blockers ; Calcium Channels ; Desoxycorticosterone ; Femoral Artery ; Heart ; Hypertension ; Neurotransmitter Agents ; Nifedipine* ; Rats* ; Renal Artery ; Silver

The study was undertaken for the clinical evaluation and statistical analysis on the 222 patients with ectopic pregnancy who had been adbitted and treated at the Mokpo St.Columban's Hospital from Jan. 1, 1993 to Dec. 31, 1995. The results were obtained as follows. 1. Hospital incidence of ectopic pregnancy wasd 1 in 45 deliveries(222/10,078). 2. Ectopic pregnancy was found to occur most frequently in the age group, ranging from 31 to 35 years(36.9%). 3. Nullipara was 26.5% and woman who had experienced artificial abortion was 69.8%. 4. Hemoglobin value over 10.0 gm/dL was in 78.8% and below 8.9 gm/dL in 4.1%. 5. Initial systolic blood pressure rise above 100 mmHg was in 75.0%, the mean value was 104.5mmHg. 6. The clinical manifestations were appeared in 64.4% from the last menstrual peroid to the next 5~8 weeks. 7. Total amount of intraperitoneal hemorrhage between 100~1,000 ml was in 62.1%, above the 1,000 ml was in 29.3% and less than 100 ml was in 8.6%. 8. Ectopic pregnancy was implanted follopain tuve in 95.0% ovary in 4.5% and cervix in 0.5%. Among tubal pregnancies, ampulla portion was involved in 76.6%, interstitial portion in 3.1%, isthmic portion in 12.2% and fimbrial portion in 3.1%. 9. In the past history, the laparoscopic tubal ligation was in 18%, peritonitis was in 1.8%, appendectomy was in 7.2% and cesarean section was in 8.6%. 10. Culdocentesis was positive in 70.0% and urinary HCG test was positive in 90%. 11. 77.8% of total cases was treated by salpingectomy, 14.9% by salpingoophorectomy and 1.4% by hysterectomy. 12. Of 222 total cases, no death occurred.
Appendectomy ; Blood Pressure ; Cervix Uteri ; Cesarean Section ; Female ; Hemorrhage ; Humans ; Hysterectomy ; Incidence ; Jeollanam-do ; Ovary ; Peritonitis ; Pregnancy ; Pregnancy, Ectopic* ; Pregnancy, Tubal ; Salpingectomy ; Sterilization, Tubal

The study was undertaken for the clinical evaluation and statistical analysis on the 222 patients with ectopic pregnancy who had been adbitted and treated at the Mokpo St.Columban's Hospital from Jan. 1, 1993 to Dec. 31, 1995. The results were obtained as follows. 1. Hospital incidence of ectopic pregnancy wasd 1 in 45 deliveries(222/10,078). 2. Ectopic pregnancy was found to occur most frequently in the age group, ranging from 31 to 35 years(36.9%). 3. Nullipara was 26.5% and woman who had experienced artificial abortion was 69.8%. 4. Hemoglobin value over 10.0 gm/dL was in 78.8% and below 8.9 gm/dL in 4.1%. 5. Initial systolic blood pressure rise above 100 mmHg was in 75.0%, the mean value was 104.5mmHg. 6. The clinical manifestations were appeared in 64.4% from the last menstrual peroid to the next 5~8 weeks. 7. Total amount of intraperitoneal hemorrhage between 100~1,000 ml was in 62.1%, above the 1,000 ml was in 29.3% and less than 100 ml was in 8.6%. 8. Ectopic pregnancy was implanted follopain tuve in 95.0% ovary in 4.5% and cervix in 0.5%. Among tubal pregnancies, ampulla portion was involved in 76.6%, interstitial portion in 3.1%, isthmic portion in 12.2% and fimbrial portion in 3.1%. 9. In the past history, the laparoscopic tubal ligation was in 18%, peritonitis was in 1.8%, appendectomy was in 7.2% and cesarean section was in 8.6%. 10. Culdocentesis was positive in 70.0% and urinary HCG test was positive in 90%. 11. 77.8% of total cases was treated by salpingectomy, 14.9% by salpingoophorectomy and 1.4% by hysterectomy. 12. Of 222 total cases, no death occurred.
Appendectomy ; Blood Pressure ; Cervix Uteri ; Cesarean Section ; Female ; Hemorrhage ; Humans ; Hysterectomy ; Incidence ; Jeollanam-do ; Ovary ; Peritonitis ; Pregnancy ; Pregnancy, Ectopic* ; Pregnancy, Tubal ; Salpingectomy ; Sterilization, Tubal

Endogenous nitric oxide(NO) plays an important role in the regulation of blood pressure. It has been known that the evoked NO-dependent dilator system may be impaired in various hypertensive models. The effects of NG-nitro-L-arginine(L-NNA), lipopolysaccharide(LPS) and tempol on mean arterial pressure(MAP) and the effects of L-NNA on isolated aorta tone were studied in order to elucidate potential alterations in resting vasodilator tone of NO in two-kidney, one clip(2K1C) hypertension. Plasma nitrite/nitrate levels were measured by colorimetric assay, and the expression of endothelial and inducible NO synthases(eNOS, iNOS) was determined by Western blot analysis. L-NNA caused an increase of MAP, while LPS produced a hypotensive effect in both 2K1C and control rats. The magnitude of the pressor or depressor response to L-NNA and LPS was comparable in the two groups. Tempol induced a sustained decrease in MAP in 2K1C rats, while it had no effects on MAP in control rats. Plasma concentrations of NO metabolites were significantly increased following the LPS-treatment in both 2K1C and control rats, while they were not affected by tempol-treatment. In endothelium-intact aortic rings precontracted with 25 mM KCl, L-NNA caused a dose-dependent contraction. The magnitude of the maximal contraction was attenuated in 2K1C rats as compared with control. An inhibition of contractile responses to L-NNA in the hypertensive group was also shown in rubbed rings, although the magnitude of contractions was markedly reduced. The vascular expression of both eNOS and iNOS was significantly decreased in 2K1C rats as compared with control. These results indicate that 2K1C hypertension is associated with a reduced basal vasodilator tone of NO and a decrease in the vascular expression of NOS isozymes.
Animals ; Aorta ; Blood Pressure ; Blotting, Western ; Hypertension ; Isoenzymes ; Nitric Oxide* ; Plasma ; Rats*

BACKGROUND: Chronic treatment with the dietary flavonoid quercetin is known to lower blood pressure and restore endothelial dysfunction in animal models of hypertension. This study investigated the direct effects of quercetin on vascular response in chronic 2-kidney, 1-clip (2K1C) renal hypertensive rats. The effects of antioxidant vitamin ascorbic acid on the vasoreactivity were also examined. METHODS: 2K1C renal hypertension was induced by clipping the left renal artery; age-matched rats that received sham treatment served as controls. Thoracic aortae were mounted in tissue baths for the measurement of isometric tension. RESULTS: Relaxant responses to acetylcholine were significantly attenuated in 2K1C rats in comparison with sham rats. Quercetin or ascorbic acid augmented acetylcholine-induced relaxation in 2K1C rats, whereas no significant differences were noted in sham rats. The relaxation response to sodium nitroprusside was comparable between 2K1C and sham rats, and sodium nitroprusside-induced relaxation was not altered by quercetin or ascorbic acid in either group. The contractile response to phenylephrine was significantly enhanced in 2K1C rats compared with sham rats. Phenylephrine-induced contraction was inhibited by pretreatment with quercetin or ascorbic acid in 2K1C rats, whereas neither chemical affected responses in sham rats. N(w)-nitro-L-arginine methyl ester markedly augmented the contractile response to phenylephrine in sham rats, whereas no significant differences were observed in 2K1C rats. Quercetin or ascorbic acid did not affect phenylephrine-induced contraction in the presence of N(w)-nitro-L-arginine methyl ester in either 2K1C or sham rats. CONCLUSION: Acute exposure to quercetin appears to improve endothelium-dependent relaxation and inhibit the contractile response, similar to the effect of ascorbic acid in 2K1C hypertension. These results partially explain the vascular beneficial effects of quercetin in renal hypertension.
Acetylcholine ; Animals ; Aorta* ; Aorta, Thoracic ; Ascorbic Acid ; Baths ; Blood Pressure ; Hypertension ; Hypertension, Renal ; Models, Animal ; Nitroprusside ; Phenylephrine ; Placebos ; Quercetin* ; Rats* ; Relaxation ; Renal Artery ; Sodium ; Vitamins