In order to provide a desirable direction for the health education on preventing smoking of elementary school students, by understanding the views held by elementary school teachers on smoking prevention education, 529 teachers at elementary schools located in Seoul were responded by constructed questionnaires during the period of Nov. 15 to Dec. 18 1999. The results of the survey analysis were as follows: 1. 91.9% of the respondents said that schools should offer education programs on preventing smoking, and this view was more strongly shared by teachers with religions than non-religious ones. 2.37.6% of teachers responded that the education should start with 4th-5th graders, while 36.0% opted for 6th grades. With regard to the appropriate class in providing such education, 41.1% selected physical education (PE), followed by extracurricular activity class with 28.8%, 45.1% of the respondents said discussion would be the most effective education method, while 39.9% recommended using experiments. fall in diseases and growth and developmental defect were pointed out as the most interesting topic for students by 33.2% and 28.1% of the surveyed, respectively. As for the most effective media for education, 57.2% recommended video, followed by computer games(19.2%) and cartoons (20.0%). 64.1% of the teachers picked school nurses as the most appropriate person in carrying out the education. The results of the finding show that it would be most desirable to provide health education on preventing smoking to senior elementary schoolers during the PE or extracurricular activity class by school nurses. The program would be structured to first show video clips on growth and developmental defect or fall in diseases resulting from smoking and to have discussions or experiments on related matters.
Child* ; Surveys and Questionnaires ; Education ; Growth and Development ; Health Education* ; Humans ; Physical Education and Training ; Seoul ; Smoke* ; Smoking* ; Child Health

In order to provide a desirable direction for the health education on preventing smoking of elementary school students, by understanding the views held by elementary school teachers on smoking prevention education, 529 teachers at elementary schools located in Seoul were responded by constructed questionnaires during the period of Nov. 15 to Dec. 18 1999. The results of the survey analysis were as follows: 1. 91.9% of the respondents said that schools should offer education programs on preventing smoking, and this view was more strongly shared by teachers with religions than non-religious ones. 2.37.6% of teachers responded that the education should start with 4th-5th graders, while 36.0% opted for 6th grades. With regard to the appropriate class in providing such education, 41.1% selected physical education (PE), followed by extracurricular activity class with 28.8%, 45.1% of the respondents said discussion would be the most effective education method, while 39.9% recommended using experiments. fall in diseases and growth and developmental defect were pointed out as the most interesting topic for students by 33.2% and 28.1% of the surveyed, respectively. As for the most effective media for education, 57.2% recommended video, followed by computer games(19.2%) and cartoons (20.0%). 64.1% of the teachers picked school nurses as the most appropriate person in carrying out the education. The results of the finding show that it would be most desirable to provide health education on preventing smoking to senior elementary schoolers during the PE or extracurricular activity class by school nurses. The program would be structured to first show video clips on growth and developmental defect or fall in diseases resulting from smoking and to have discussions or experiments on related matters.
Child* ; Surveys and Questionnaires ; Education ; Growth and Development ; Health Education* ; Humans ; Physical Education and Training ; Seoul ; Smoke* ; Smoking* ; Child Health

Water transport is mediated by two distinct pathways, diffusional and channel-mediated water transport. The first molecular water channel was identified from human erythrocytes in 1992. Genetically-related proteins from other mammalian tissues have subsequently been identified to transport water, and the group is referred to as the "Aquaporins". Aquaporin-4 (AQP4) is most abundant in the brain, which may be involved in CSF reabsorption and osmoregulation. However, ontogeny and regulatory mechanisms of AQP4 channels have not been reported. Northern blot analysis showed that AQP4 mRNA began to be expressed in the brain just before birth and that its expression gradually increased by PN7 and then decreased at adult level. AQP4 was expressed predominantly in the ependymal cells of ventricles in newborn rats. And then its expression decreased in ependymal cells and increased gradually in other regions including supraoptic and paraventricular nuclei. AQP4 is also expressed in the subfornical organ, in which the expression level is not changed after birth. Cryogenic brain injury did not affect expression of AQP4 mRNA, while ischemic brain injury decreased it. Osmotic water permeability of AQP4 channel expressed in Xenopus oocytes was inhibited by the pretreatment of BAPTA/AM and calmidazolium, a Ca2+/ Calmodulin kinase inhibitor, in a dose-dependent manner. These results indicate that the expression and the function of AQP4 channel are regulated by developmental processes and various pathophysiological conditions. These results will contribute to the understanding of fluid balance in the central nervous system and the osrmoregulatory mechanisms of the body.
Adult ; Animals ; Blotting, Northern ; Brain Injuries ; Brain* ; Calcium-Calmodulin-Dependent Protein Kinases ; Central Nervous System ; Diffusion ; Erythrocytes ; Humans ; Infant, Newborn ; Ischemia* ; Oocytes ; Osmoregulation ; Parturition ; Permeability ; Rats ; RNA, Messenger ; Subfornical Organ ; Water* ; Water-Electrolyte Balance ; Xenopus

To determine molecular mechanisms of Aquaporin-CD (AQP2) gene regulation, the promoter region of the AQP2 gene was examined by transiently transfecting a promoter-luciferase reporter fusion gene into mouse renal collecting duct cell lines such as mIMCD-3, mIMCD-K2, and M-1 cells, and NIH3T3 mouse embryo fibroblast cells. PCR-Southern analysis reveals that mIMCD-3 and mIMCD-K2 cells express AQP2, but M-1 and NIH3T3 cells do not, and that the treatment with cpt-cAMP (400 muM) or forskolin/isobutylmethylxanthine (IBMX) increased the AQP2 expression in IMCD cells. In both IMCD and NIH3T3 cells, the constructs containing the promoter of AQP2 gene showed promoter activities, indicating lack of tissue-specific element in the 1.4 kb 5'-flanking region of the mouse AQP2 gene. Luciferase activity in the IMCD cells transfected with the construct containing 5-flanking region showed responsiveness to cpt-cAMP, indicating that the 1.4 kb 5'-flanking region contains the element necessary for the regulatory mechanism by cAMP. The promoter-luciferase constructs which do not have a cAMP-responsible element (CRE) still showed the cAMP responsiveness in IMCD cells, but not in NIH3T3 cells. Increase in medium osmolarity did not affect AQP2 promoter activity in mIMCD-K2 cells. These results demonstrate that AQP2 gene transcription is increased with cAMP treatment through multiple motifs including CRE in the 5'-flanking region of the gene in vitro, and the regulatory mechanism may be important for in vivo regulation of AQP2 expression.
Animals ; Aquaporin 2 ; Cell Line ; Embryonic Structures ; Fibroblasts ; Luciferases ; Mice* ; Osmolar Concentration ; Promoter Regions, Genetic