No abstract available.
Pneumoconiosis* ; Thorax*

In situ hybridization (ISH) is a standard method for localizing DNA or RNA sequences in tissue or cell preperation. The technique was developed at the electron-microscopic level, and enables the precise subcellular localization. A method was developed for detection of specific viral DNA. We have tested various methods and technique to detect specific viral DNA through ISH at the electron microscopic level. Postembedding method of ultrastructural ISH was developed and successfully applied for the detection of human papillomavirus type 16 in squamous cell carcinoma of the uterine cervix and Epstein-Barr virus in EBV-infected leukemia cell line. The following results are made. The best results were obtained using 0.2% glutaraldehyde and 4% paraformaldehyde fixed tissue or cell block. The labelling was best observed on Unicryl resin and Lowicryl K4M resin sections. Epon sections showed no reactivity. Thin sections of Unicryl resin were more easier than Lowicryl K4M resin. Enzymatic predigestion with proteinase K, pepsin and trypsind gave good results. However, high concentration of these produce poor results due to excessive destruction of the cellular components. Alkali treatment with 0.5N sodium hydroxide produced successful results in denaturation of target DNA. The labelling density of gold particles was independent of incubation time or temperature in hybridization step. The viral DNA labelling was localized mainly within the nucleus, both within and at the edge of electron dense regions, and below the nuclear membrane. And the labelling was seen in the form of a dense, roughly spherical shape. In conclusion, the best results are obtained by the conditions that tissue fixed by 0.2% glutaraldehyde and 4% paraformaldehyde solution, embedded with Unicryl resin, protein denaturation by 0.1ul/ml proteinase K, DNA denaturation by 0.5N sodium hydroxide, and reaction with DNA probe.
Humans

A clinical observation and comparision was carried out on 466 cases of meningitis in infancy and childhood : 135 cases of purulent meningitis ; 208 cases of aseptic meningitis, and 123 cases of tuberculous meningitis. They were admitted to Jeonju Presbyterian Medical Center from January, 1973 to December, 1979. The results were as follows; 1) Male to female ratio was 2.1:1 in purulent meningitis, 3.2:1 in aseptic meningitis, and 2.4:1 in tuberculous meningitis. 2) The seasonal peak incidence was Spring and Summer for purulent and tuberculous menigitis and Summer for aseptic meningitis. 3) The most predilectional age which resulted in meningitis was infancy in purulent meningitis, 4 to 12 years o fage in aseptic meningitis and 1 to 4 years of age in tuberculous meningitis. 4) Fever was the most frequent symptom ; and vomiting, convulsion, unconsciousness, and headache followed in that order in the three kinds of meningitis. The most frequent neurologic finding was stiff neck; and Kernig's sign, Brudzinski's sign, and Babinski's sign followed in that order in the three kinds of meningitis. 5) In CSF examination on admission, cell counts were most frequently under 1,000/mm3 in purulent meningitis(49.6%), under 50/mm3 in aseptic menigitis (35.6%) and 100 to 300/mm3 in tuberculous meningitis (52.0%). The sugar level in 74.1% of purulent meningitis and in 84.5% of tuberculous meningitis was under 50mg/dl. In aseptic meningitis the sugar level was over 50mg/dl in 72.6%. The protein level was most frequently 30 to 150mg/dl in all three kinds of meningitis. 6) The mortality rate was 14.1% in purulent meningitis, 0.5% in asepic meningitis and 10.6% in tuberculous meningits. 7) The younger the patient in purulent and tuberculous meningitis, the worse the prognosis. The mortality rate of tuberculous meningitis was 9.1% in stage II and 50% in stage III.
Cell Count ; Female ; Fever ; Headache ; Humans ; Incidence ; Jeollabuk-do ; Male ; Meningitis* ; Meningitis, Aseptic ; Mortality ; Neck ; Neurologic Manifestations ; Prognosis ; Protestantism ; Reflex, Babinski ; Seasons ; Seizures ; Tuberculosis, Meningeal ; Unconsciousness ; Vomiting

The digital dust indicator (Sibata P-5), one of the direct-reading instruments was evaluated for the respirable dust in the underground coal mine environments. As a reference, respirable dust was determined using three cyclones and/or impactors. All the tests were performed on aerosol in twenty underground coal mines. The coefficients of mass-relative concentration were 0.067+/-0.054 (Mean+/-Standard deviation) (range: 0.006-0.172). The relationship between relative concentration and temperature was not significant statistically. Also, the relationship of relative concentration and relative humidity was not significant. Mass concentration and relative concentration were 5.31+/-5.22 mg/m3 and 162+/-163 CPM (Mean+/-Standard deviation) respectively. The range of mass concentration was 1.22-22.69 mg/m3; relative concentration 16-628 CPM. The relationship of mass concentration and relative concentration was not significant in these ranges.
Coal ; Cyclonic Storms ; Dust* ; Humans ; Humidity

No abstract available.
Arthroplasty* ; Knee*

Lead is one of the most widespread environmental toxins and its poisoning in children was considered a rarity, usually resulting from unique circumstances such as inappropriate use of leadbased body cosmetics or direct administration of lead-containing folk medicines. The increasing concern about children with lower levels of lead exposure has developed, but there are no data regarding the mean blood lead levels and the incidence of symptomatic or asymptomatic lead poisoning in Korean children. We analyzed the zinc protoporphyrine (ZPP) values and blood lead concentrations in 163 pediatric inpatients for a prospective study of lead exposure. The blood lead concentrations in all 163 children were 15~54 g/dl, of whom 111 children (68.1%) were 25~54 g/dl which needs decision to chelate based on the EDTA provocation test. Among 111 children whose blood lead concentrations are 25~54 g/dl, 59 children(53.2%) were between 7 months and 3 years of age, which revealed no significant higher incidence of lead exposure than any other age group. The ZPP values in 126 children (77.3%) were above 35 g/dl. The mean blood lead concentration and ZPP values are 27.8 g/dl and 48.8 g/dl, respectively. We conclude that there are many asymptomatic children with increased absorption of lead in the urban area of Korea, and we need further studies regarding lead poisoning. It is important that there must be a national counterplan and that pediatricians continue to pay attention to lead posioning in children.
Absorption ; Child ; Edetic Acid ; Humans ; Incidence ; Inpatients* ; Korea ; Lead Poisoning ; Poisoning ; Prospective Studies ; Zinc

No abstract available.
Nails, Malformed* ; Pachyonychia Congenita*

No abstract available.
Nails, Malformed* ; Pachyonychia Congenita*

No abstract available.
Primary Myelofibrosis*

This study was performed to fine out the possible causes of primary infertility in female and to provide some diagnostic and therapeutic modalities. A total of 104 cases of endometrium in primary infertility was obtained from the pathology files of Chosun University Hospital and Kwang-ju Christian Hospital during the period of 5 years and 10 months from January, 1984 to October, 1989. The endometrial biopsies were taken on the first of menstruation and classified according to Noyes, Hertig and Rock's criteria. Histologic findings which were compatible with their normal menstrual cycle (premenstrual of late secretory phase and bleeding phase) were noted in 52 cases (50%). Abnormal 52 cases (50%) revealed deficient secretory phase in 36 case (34.6%), proliferative phase in 5 cases (4.8%), irregular proliferation and chronic nonspecific endometritis in 4 cases (3.8%), respectively, endometrial hyperplasia in 3 cases (2.9%), tuberculous endometritis in 2 cases (1.9%), and asynchronous cycle in 1 case (1%). Among the case of dissociated delay was 21 cases (20.2%) and coordinated delay was 15 cases (14.4%). Therefore, deficient secretory phase with dissociate delay was most common abnormal endometrial finding in cases of female primary infertility. The peak age distribution of the female primary infertility in this study revealed 73 cases (63.8%) in patients between the age of 25 and 29. This finding indicated that there was no significant relationship between the age distributions and the endometrial histopathologic findings.
Female ; Humans ; Biopsy