Animals ; Mice ; Colon ; Histological Techniques

retrospective study include 22 patients with Meckel diverticulum were found incidentally or because of its complications, between 1992-2002 in Binh Dan hospital, HCM city. Use the Fisher exact test with two tails to determine the correlation between the histological property and its clinical presentations. The results showed that the features of Meckel diverticulum were noted, no difference in sex. The histological type of the mucosa (gastric) had significant positive correlation with the clinical presentation. Resection of the unexpected Meckel diverticulum could be performed safely with a low complication rate
Meckel Diverticulum ; Models, Anatomic ; Histological Techniques ; surgery

Rectal suction biopsy with acetylcholinesterase (AChE) histochemistry has gained increased acceptance as the means of definitely diagnosing Hirschsprung's disease (HD) as well as of excluding this diagnosis when evaluating children with low intestinal obstruction or chronic constipation since the report of Meier-Ruge et al. in 1972. But this AChE histochemical study has not been reported yet in Korea. During the 14-month period from April, 1991 through June, 1992, 37 children, aged 3 days to 17 years had rectal suction biopsies for the diagnosis or exclusion of HD. In this study, AChE histochemistry (N = 37) was compared with hematoxylin and eosin (H and E) staining of same suction biopsy specimens (N = 35) for diagnostic accuracy. The histochemical criterion used for the diagnosis of Hirschsprung's disease was that of Chow et al. (1977), i.e., the presence of many coarse discrete cholinergic fibers in the muscularis mucosae and in the immediately subjacent submucosa regardless of an infiltration of cholinergic fibers in the lamina propria. Of 13 biopsies from the patients with Hirschsprung's disease (N = 13), there were 12 positive reactions, and one false negative reaction in a neonate with total colonic aganglionosis. All biopsies from 24 unaffected children demonstrated negative reactions with no false positive reaction. In comparison, of the 35 specimens examined by H and E staining, ganglion cells were present in the submucosal Meissner's plexus only in 15 of these 24 unaffected children. In conclusion, a 97% diagnostic accuracy was achieved with AChE histochemistry compared with a 74% accuracy with H and E staining (P < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
Acetylcholinesterase/*analysis ; Adolescent ; Biopsy/methods ; Child, Preschool ; Female ; Hirschsprung Disease/enzymology/*pathology ; Histocytochemistry ; Histological Techniques ; Humans ; Infant ; Infant, Newborn ; Male ; Predictive Value of Tests ; Rectum/enzymology/*pathology ; Suction

44 patients of chronic B hepatitis are divided into two groups of 22 patients. The one is treated with lamivudine (Jefflix) 100 mg daily in 12 months continuously and the other with usual vitamin and liver tonics. Results showed in 72.7% patients of lamivudine group, the indices of histological activities were improved (through Knodell score), while in controlled group – 31.8% and over the half of this group these indices worsened. Thus, lamivudine has got good effect
Histological Techniques ; Hepatitis B ; Patients ; Lamivudine ; drugs ; Therapeutics

Among 156 patients with ages of 15-80, suffering the major salivary cancer in which parotid salivary cancer (73.5%), mandibullar salivary (26.5%), 15% of patients had symptoms of paralysis of peripheral facial nerve. 9.6% of patients had persistent pain. The patients received an operation for selective ganglion curettage. If the ganglion was positive, postoperative radiation indicated for both tumor and ganglion at dose of 70 Gy. If the ganglion was negative, the postoperative radiation indicated for primary tumor at dose of 70 Gy. Results: the total survival rate after 3 years and 5 years of the treatment was 74.1 and 60.6%, respectively. The local relapse rate after the single operation, radiation at dose of 50 Gy and dose of 70 Gy was 48.1%, 71.4% and 3% respectively
Salivary Gland Diseases ; Histological Techniques ; diagnosis ; therapeutics ; Salivary Gland Neoplasms

This study was conducted to underscore the spatial distribution of some biologically active proteins within the epididymal duct in the dromedary camel. Paraffin-embedded sections from different regions of epididymis were stained by conventional histological techniques and by immunohistochemistry. A battery of primary antibodies against six proteins (S100, alpha smooth muscle actin [alpha-SMA], connexin-43 [Cx43], galactosyltransferase [GalTase], angiotensin converting enzyme [ACE], and vascular endothelial growth factor [VEGF]) were used. The epididymal epithelium consisted of five cell populations: principal, basal, apical, dark, and halo cells. The histochemical findings indicated the absence of binding sites for VEGF and Cx43. The principal cells (PCs) showed variable immunoreactivity (IR) for ACE, S100, and GalTase throughout the whole length of the duct. The apical surfaces of most PCs (at the caput) and some PCs (at the corpus) exhibited intense ACE-IR, whereas those at the cauda displayed alternating negative and strong immunostaining. Similarly, moderate S100-IR was found in cytoplasm and nuclei of all PCs at the caput, few PCs at the corpus, and several PCs alternating with negative PCs at the cauda. In contrast, only some PCs showed weak to strong GalTase-IR in different regions. Apart from negative to weak positive S100-IR, basal cells failed to show IR for all other proteins. Apical cells displayed strong IR for ACE, S100, and GalTase with some regional differences. The peritubular and vascular smooth muscle cells revealed strong alpha-SMA-IR in all regions. In conclusion, the spatial distribution of different proteins in camel epididymis showed similarities and differences to other mammalian species. The region-specific topographic distribution of different proteins and cell types might indicate that the caput and cauda are metabolically more active than that of the corpus.
Actins ; Antibodies ; Binding Sites ; Camels ; Connexin 43 ; Cytoplasm ; Epididymis ; Epithelium ; Histological Techniques ; Immunohistochemistry ; Male ; Muscle, Smooth ; Muscle, Smooth, Vascular ; Peptidyl-Dipeptidase A ; Proteins ; Vascular Endothelial Growth Factor A

The Golgi complex is the central organelle of the secretory pathway and has many complicate functions. The endeavours to isolate and purify the Golgi apparatus from cultured cells will benefit further investigation of Golgi. A large number of gastric cancer cells SGC7901 were cultivated in vitro, then Golgi apparatus were isolated from the cells by differential centrifugation combined with sucrose density gradient ultra-centrifugation. Its purity was characterized biochemically by enzymatic assays, morphologically by electron microscopy (EM) and neutral red supravital staining. Finally the Golgi complex was successfully fractionated from gastric cancer cells SGC7901. The first successful isolation of Golgi apparatus from gastric cancer cells SGC7901 by using ultra-centrifugation will lead to research into the function of Golgi apparatus.
Cell Line, Tumor ; Golgi Apparatus ; ultrastructure ; Histological Techniques ; Humans ; Stomach Neoplasms ; pathology ; ultrastructure

PURPOSE: Metastasis of a colorectal carcinoma to regional lymph nodes indicates poor prognosis. The detection of lymph node metastasis is routinely performed by his topathological analysis of hematoxylin-eosin (H&E) stained sections. However the routine histological technique may fail to detect isolated tumor cells in lymph nodes. The aims of this study are to elucidate the prognostic significance of the presence of isolated tumor cells in the regional lymph nodes in colorectal cancer, and to elucidate the correlation between the presence of isolated tumor cells and p53 protein expression in the primary colorectal cancer tissue. METHODS: We used immunohistochemical staining with anti-cytokeratin antibody to examine 452 lymph nodes in 24 patients (11 recurrent and 13 nonrecurrent) who were histologically determined Astler-Coller B. And we used immunohistochemical staining with p53 protein to examine primary colorectal cancer tissues of the patients. RESULTS: Immunohistochemical staining of cytokeratin revealed the presence of isolated tumor cells in 5/13 patients (38.5%), 5/214 lymph nodes (2.34%) in the nonrecurrent group and 6/11 patients (54.6%), 11/244 lymph nodes (4.51%) in the recurrent group, respectively. The detection rate of isolated tumor cells in the recurrent group was slightly higher than nonrecurrent group, but the difference was not significant statistically. The expression rate of p53 protein was 23.1% (3/13) in the nonrecurrent group and 36.4% (4/11) in the recurrent group, respectively. The expression rate of the p53 protein was not significantly correlated with the presence of isolated tumor cells in regional lymph nodes and the rate of tumor recurrence. CONCLUSIONS: The presence of isolated tumor cells in regional lymph nodes was not a prognostic indicator in predicting recurrence in histologically determined Astler- Collar B colorectal cancer patients.
Colorectal Neoplasms* ; Histological Techniques ; Humans ; Keratins ; Lymph Nodes* ; Neoplasm Metastasis ; Prognosis ; Recurrence

Biopsy ; Histological Techniques ; instrumentation ; methods ; Humans ; Indicators and Reagents ; Paraffin Embedding ; Ultrasonics

The calcium-sensitive neutral protease, calpain, is activated in the basilar artery after subarachnoid hemorrhage. Pathological activation of this proteolytic enzyme has been suggested to contribute to cerebral vasospasm after subarachnoid hemorrhage. The present study was undertaken to evaluate the effects of a newly developed calpain inhibitor, CX-287 on vasospasm. A blind, randomized trial was utilized in which CX-287 was injected intravenously into subarachnoid hemorrhage rabbits. Two days after subarachnoid hemorrhage, animals were sacrificed by perfusion-fixation and cross-sectional areas of the basilar arteries were measured using histological techniques. Expressing the cross-sectional area in the untreated SAH animals as a percentage of control value, it was 38.4+/-5.7%. Basilar artery area of the treatment groups with 1.5mg/kg CX-287(b.i.d. or t.i.d) showed no statistical differences from subarachnoid only group(b.i.d.: 34.7%, t.i.d.: 49.0%). However, the treatment group with 3mg/kg CX-287 showed significant reversal of the subarachnoid hemorrhage-induced constriction(b.i.d.: 73.4%, p<0.0003, t.i.d.: 58.7%, p<0.05). These findings support the important role of calcium activated proteolysis by calpain in the pathophysiology of vasospasm after subarachnoid hemorrhage. Furthermore, these results provide the first demonstration that a calpain inhibitor can inhibit cerebral vasospasm.
Animals ; Basilar Artery ; Calcium ; Calpain* ; Histological Techniques ; Injections, Intravenous ; Proteolysis ; Rabbits ; Subarachnoid Hemorrhage* ; Vasospasm, Intracranial