1.Identification of Bacterial Flora other than Bacillus sp. from Bentong Hot Spring in Pahang, Malaysia
Abdul Hamid Abdul Aziz ; Nur Fariza Mat Reffin ; Mohamed Kamel Abd Ghani ; Hing Hiang Lian ; Ahmad Zorin Sahalan
Malaysian Journal of Health Sciences 2012;10(2):53-55
A study on the isolation and identification of bacterial flora from hot spring was carried out using water from Bentong
hot spring in Pahang. In general most of the bacteria identified from the hot springs were from Bacillus sp. This was
due to its ability to adapt to high temperature mainly by forming endospore. The objective of this study was to identify
bacteria other than Bacillus that were able to survive in hot springs. Three hot spring pools were identified (A,B and C)
with temperatures ranging from 36°C to 52°C and pH values between 7.88-8.65. This study has succesfully isolated two
species of Gram positive cocci and three species of Gram negative bacilli. The two Gram positives were Streptococcus sp.
and Staphylococcus sp. The three species of Gram negative rods were unidentified and were denoted as X, XI and XII.
2.Identification of Gram Positive and Negative Bacteria Microflora Isolated from Hot Water Spring in Bentong, Pahang
Ahmad Zorin SAHALAN ; Abdul Hamid Abdul Aziz ; Mohamed Kamel Abd Ghani ; Hing Hiang LIAN
Malaysian Journal of Health Sciences 2018;16(2):1-4
A study on the isolation and identification of bacterial flora from hot spring was carried out from Bentong hot springwater, Pahang. In general, many of the bacteria identified from the hot spring were from Bacillus sp. due to its abilityto adapt to high temperature mainly by forming endospore. The objective of this study was to identify bacteria otherthan Bacillus sp. that were able to survive this hot spring. Three hot spring pools were identified as A, B and C. Thetemperatures were ranging from 36°C to 52°C and their pH values were between 7.88-8.65. The result shows thattwo species of Gram positive cocci and three species of Gram negatives were isolated. The two Gram positives wereStreptococcus sp. and Staphylococcus sp. The three species of Gram negative rods were identified as Pseudomonas sp.,Klebsiella sp. and Proteus sp. In conclusion, non-sporulating species of bacteria other than Bacillus sp. have adaptedto survive in hot spring