OBJECTIVETo investigate the codon-72 polymorphism of the tumor suppressor gene p53, codon-72 encodes arginine (Arg) or proline (Pro) for a genetic predisposition,to keloid or hypertrophic scar.

METHODSThe distribution of codon 72 polymorphism of p53 gene was analyzed from the 54 keloid and 30 hypertrophic scar(HS)of the Japanese patients with restriction fragment length polymorphism analysis and DNA sequence analysis.

RESULTSThe frequency of the Proline-encoding alleles and Arginine-encoding alleles in the hypertrophic scar patients and the piercing-induced ear-lobe keloid patients, was deviated significantly from that in the normal Japanese controls.

CONCLUSIONSThe Proline-encoding allele and Arginine-encoding allele could have the risks for the hypertrophic scar and the piecing-induced ear-lobe keloid. Also, the pathogenesis of the hypertrophic scar seems to be different from that of keloid at the molecular level.

Cicatrix, Hypertrophic ; genetics ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; Genotype ; Humans ; Keloid ; genetics ; Male ; Polymorphism, Genetic ; Tumor Suppressor Protein p53 ; genetics

OBJECTIVETo identify the expression of protein which is characteristic of stem cell, induce the adipogenic differentiation in vitro by ASCs harvested from GFP transgenic mice, and to assess the possibility of constructing adipose tissue via the attachment of ASCs to type I collagen scaffold.

METHODSInguinal fat pads from GFP transgenic mice were used for the isolation of ASCs. Expression of CD29, CD34, CD45, CD133 and HLA-DR were detected by flow cytometry. After expansion to three passages, the ASCs were incubated in an adipogenic medium for two weeks. Then they were attached to collagen I scaffold and co-cultured for 12 hours, followed by transplantation under the dorsal skin of athymic mice for 2 months. Adipogenic differentiation of ASCs in vitro was assessed by morphological observation, Oil red O staining and newly formed tissue was detected by HE staining.

RESULTSThe cultured stem cells were fibroblast-like cells and showed highly homogeneous appearance with active proliferation capacity. Stem cells' characteristic CD expression was proved. After being incubated in an adipogenic medium, they could differentiate into mature adipocytes. Accumulation of lipid droplets in the cytoplasm was testified by Oil red O staining, morphological and biological observation. 0.5 ml new tissue was formed and was confirmed by fluorescent observation and HE staining to be mature adipose tissue.

CONCLUSIONSAdipose derived stem cells can successfully differentiate into mature adipocytes exhibiting an adipose-like morphology and expression of intracytoplasmic lipid droplet. It was an efficient model for adipose tissue engineering with ASCs and type I collagen scaffold.

Adipocytes ; cytology ; Adipose Tissue ; cytology ; Animals ; Cell Culture Techniques ; Cell Differentiation ; Cells, Cultured ; Female ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Transgenic ; Stem Cells ; cytology ; Tissue Engineering ; methods