1.Phytochemical screening and antioxidant activity of Lebanese Eryngium creticum L.
Hussein FARHAN ; Fatima MALLI ; Hassan RAMMAL ; Akram HIJAZI ; Ali BASSAL ; Nawal AJOUZ ; Bassam BADRAN
Asian Pacific Journal of Tropical Biomedicine 2012;(z3):1217-1220
Objective: To determine the phytochemical screening and quantification of total phenolics contents in fresh Eryngium creticum (E. creticum) leaves and stems extract and to evaluate its total antioxidant activity. Methods: Quantification of total phenolics contents in fresh E. creticum leaves and stems extract and evaluation of its total antioxidant activity, were done using the spectrophotometric analyses. Results: The consumption of 100 g of fresh E. creticum leaves and stems could provide antioxidants equivalent to (78.50±0.80) mg of vitamin C and (50.42±0.50) mg of vitamin C, respectively. Conclusions: From this study, it can be concluded that E. creticum can be interesting to prevent diseases directly linked to oxidative stress.
2.Chemical composition and antioxidant activity of a Lebanese plant Euphorbia macroclada schyzoceras
Hussein FARHAN ; Hassan RAMMAL ; Akram HIJAZI ; Ahmad DAHER ; Mohamad REDA ; Hussein ANNAN ; Ali CHOKR ; Ali BASSAL ; Bassam BADRAN
Asian Pacific Journal of Tropical Biomedicine 2013;(7):542-548
Objective:To determine the chemical composition, total phenolic and total flavonoid contents of the crude extracts from leaves and stems of a Lebanese plant Euphorbia macroclada schyzoceras (E. macroclada), and to evaluate their antioxidant potential using DPPH, H2O2, and chelating of ferrous ions tests. Methods:Quantification of the total phenolic and total flavonoid contents of the crude extracts from leaves and stems and the antioxidant activities were evaluated using spectrophotometric analyses. The chemical composition has been estimated using different techniques such as IR, LC/MS and NMR. Results:Ethanolic extract from leaves of E. macroclada was better than aqueous extract and showed higher content in total phenolic and total flavonoid than found in the stems. On the other hand, using DPPH and H2O2 tests, this extract from leaves showed higher antioxidant capacity than aqueous extract. However, using the chelating of ferrous ions test, the antioxidant activity of the aqueous extract of both stems and leaves was stronger than that of ethanolic once. The chemical composition of the whole plant showed the presence of some aromatic compounds and fatty acids. Conclusions:Both ethanolic and water extracts from both parts of this plant are effective and have good antioxidant power. So, this plant can be used in the prevention of a number of diseases related to oxidative stress.
3. Efficiency of combining pomegranate juice with low-doses of cisplatin and taxotere on A549 human lung adenocarcinoma cells
Nasser MOHAMAD ; Hijazi AKRAM ; Sayed BOUCHRA ; Jamal ZEINAB ; Ibrahim SAJIDA ; Rammal HASSAN ; Sayed BOUCHRA ; Al Rekaby ABD-EL-AMEER ; Nasser MOHAMMED
Asian Pacific Journal of Tropical Biomedicine 2018;7(1):19-24
Objective: To test the coalescence effect of two chemotherapy drugs at low effective dose (cisplatin and taxotere) combined with pomegranate juice on A549 cancer cells. Methods: Infrared spectroscopy method is a qualitative test that was performed to ensure the existence of the phytochemicals providing the antioxidant activity through the presence of the hydroxyl group (-OH). The viability of A549 cell line and normal MCs was tested using the neutral red uptake, Clonogenic survival, XTT and Cell migration assays. Results: Our results showed that this combination firstly led to a greater decrease in the viability of cells comparing to those treated with chemotherapy drugs alone, and secondly led to a significant reduction in cell migration. Conclusions: These data suggest a synergistic effect between the pomegranate and cisplatin which makes probably this combination a powerful option for treating lung adenocarcinoma and in parallel minimizing the systemic side effects.