Introduction: Melioidosis is endemic in Malaysia but prevalence data is limited. Methodology: In this cross-sectional study, sera suspected for melioidosis were received from local hospitals from year 2013 to 2014. These data were grouped into different age groups with more focus in children aged <15 years old. Results: Children <15 years old were more exposed to Burkholderia pseudomallei [Adjusted Odds ratio (AOR)=4.71, 95% Confidence Interval (CI): 4.04, 5.50) compared to the other age groups. Females, instead of males, had a slightly higher risk for melioidosis. There were more seropositive cases against melioidosis in eastern coast states (Kelantan, Terengganu, Pahang) and Sarawak. Sabah natives and nonMalaysians had higher risk of exposure to B. pseudomallei. Conclusion: Age group and region were independent risk factors for exposure against B. pseudomallei. Paediatric melioidosis is of concern and a marker against intensity of exposure.