Influence of sulphur hot spring water upon wound healing of rat skin was studied. To provide local inflammation, croton oil was injected intradermally into a previously shaved area of rat abdominal skin. Those rats were taken bath with sulphur hot spring water at 37°C for 10min a day, every second day. The increased ratio of hexosamine to hydroxyproline was observed in skin necrotized with croton oil at 4th day, because of increases in hexosamine and decreases in hydroxyproline concentrations with inflammation. After that, hexosamine and hydroxyproline concentrations of necrotic skin returned to control, uninjured levels in 10 days. In bathing with sulphur hot spring water, changes in hexosamine and hydroxyproline concentrations of necrotic area did not alter to bathing with deionized water and to non-bathing of rats.
Although influence of bathing with sulphur hot spring water was examined on glutathione metabolism in rat liver and kidney, it was observed that reduced glutathione concentration, and GSH-reductase, GSH-peroxidase and γ-glutamyl transpeptidase activities unchanged in liver and kidney for the bathing period of 14 days.

The effect of oral administration of sulphur hot spring water on chronic liver damaged rat was studied. The liver damaged rats were produced by injection of carbon tetrachloride (150l of CCl₄ per 100g of body weight) twice a week. Sulphur hot spring water was administrated ad libitum as drinking water throughout experimental period. At 40 and 88 days, rats were sacrificed for histological and biochemical examinations.
When tap water was administrated to CCl₄-poisoning rats for 88 days, extensive vacuolar degeneration of parenchymal cells were observed in liver. While in administration of sulphur hot spring water, there were mild vacuolar degeneration in parenchymal cells of liver.
Although GSH content and GSH-peroxidase activity of liver unchanged in CCl₄-poisoning, and were unaffected with administration of sulphur hot spring water, GSH-reductase activity increased with administration of sulphur hot spring water for 88 days. The increased lipoperoxide and hydroxyproline in liver with CCl₄-poisoning were slightly lowered by administration of hot spring water.
In plasma, furthermore, LDH, GPT and GOT activities which increased markedly by CCl₄-poisoning decreased strikingly by administration of hot spring water at 88 days. Whereas ALP, CE and LAP activities little changed by CCl₄-poisoning, and were scarcely affected with administration of hot spring water. Among other plasma components, though total cholesterol and cholesteryl ester levels decreased by CCl₄-poisoning, those levels were not sustained with sulphur hot spring water administration.