OBJECTIVE: Increasingly it is being recognized that genetic factors play a significant role in causing malformation. There are many available prenatal diagnostic methods including cytogenetic karyotyping using amniocentesis and cordocentesis, fluorescence in situ hybridization(FISH), and primed in situ labelling(PRINS). Our purpose was to attempt to discuss the clinical use of cytogenetic karyotyping, FISH, and PRINS. METHODS: We conducted 222 cases of cytogenetic karyotyping using amniocentesis and cordocentesis, l0 cases of FISH, and 10 cases of PRINS from January 1996 to July 1998 at Ewha Womans University Mokdong Hospital. Age distribution, chromosomal abnormalities by age group, indication, karyotype, and baby outcomes were performed. RESULTS: Overall incidence of chromosomal abnormalities was 7.7%(17cases) and chromosomal abnormalities were most frequently noted in 30-34 year old women and 35-39 year old women(2.3%, respectively). Among 222 cases, 25-29 year old women were highest(30.2%). Chromosomal abnormalities among cytogenetic karyotyping cases were Down syndrome, Edward syndrome, Patau syndrome, Deletion(8), Inversion(9), etc. The 5 cases of healthy baby among chromosomal abnormalities were delevered. Among 213 cases of karyotyping using amniocentesis, abnormal karyotyping cases were 15 cases. Among 15 cases, 8 cases were terminated and 5 cases of healthy baby were delivered. Among 9 cases of karyotyping using cordocentesis, 2 cases of chromosomal abnormalities(Edward, Down syndrome) were found and 3 cases healthy baby were delivered. Among 10 cases of FISH results, 6 case of FISH results were the same with G-banding and were different from G-banding. Among 10 cases of PRINS results, we got the PRINS results from 7 cases. CONCLUSION: It is concluded that cytogenetic karyotyping, FISH, and PRINS are very useful to detect chromosomal abnormalities.
Age Distribution ; Amniocentesis ; Chromosome Aberrations ; Cordocentesis ; Cytogenetics* ; Down Syndrome ; Female ; Fluorescence* ; Humans ; In Situ Hybridization* ; Incidence ; Karyotype ; Karyotyping* ; Prenatal Diagnosis* ; Primed In Situ Labeling

No abstract available.
Cervix Uteri* ; Chlamydia trachomatis* ; Chlamydia* ; Female

A clinical observation was made on the 236 patients who had undergone transurethral resection of bladder tumor(TUR) between Jan. 1990. and Feb. 1996. in the Department of Urology, Korea Cancer Center focusing on complications. The mean operating time was 59.0 minutes(n=400). The most common immediate complication was bleeding(1.8%), and the remainder was nonurologic fever(0.8%). The delayed complication was urethral stricture(1.0%) requiting visual urethrotomy. The immediate postoperative morbidity was 2.5% and risk factors for immediate morbidity after TUR were operating time longer than 60 minutes and tumor, invading the muscle or more deeply(p<0.05, by Chi-square test). The delayed morbidity was 1. 0% and the only risk factor for delayed morbidity after TUR was the number of ~IJR more than 2(p<0.05, by Chi-square test). Careful attention to surgical details and indications is needed to reduce the amount and significance of the postoperative morbidity.
Humans ; Korea ; Risk Factors ; Urinary Bladder Neoplasms* ; Urinary Bladder* ; Urology

A clinical observation was made on the 236 patients who had undergone transurethral resection of bladder tumor(TUR) between Jan. 1990. and Feb. 1996. in the Department of Urology, Korea Cancer Center focusing on complications. The mean operating time was 59.0 minutes(n=400). The most common immediate complication was bleeding(1.8%), and the remainder was nonurologic fever(0.8%). The delayed complication was urethral stricture(1.0%) requiting visual urethrotomy. The immediate postoperative morbidity was 2.5% and risk factors for immediate morbidity after TUR were operating time longer than 60 minutes and tumor, invading the muscle or more deeply(p<0.05, by Chi-square test). The delayed morbidity was 1. 0% and the only risk factor for delayed morbidity after TUR was the number of ~IJR more than 2(p<0.05, by Chi-square test). Careful attention to surgical details and indications is needed to reduce the amount and significance of the postoperative morbidity.
Humans ; Korea ; Risk Factors ; Urinary Bladder Neoplasms* ; Urinary Bladder* ; Urology

A case of recurrent fibrosarcoma arising from the distal portion of the left spermatic cord in a twenty-two-year-old male is presented, and is discussed on its incidence, differential diagnosis, treatment and prognosis with brief literature review.
Diagnosis, Differential ; Fibrosarcoma* ; Humans ; Incidence ; Male ; Prognosis ; Spermatic Cord*

An autopsy case of spontaneous rupture of urinary bladder followed by longstanding chronic and acute hemorrhagic cystitis associated with anomalous course of the right ureter and pyo-hydronephrosis, and acutely disseminated tuberculous pneumonia in a twenty-two-year-old Korean Army soldier is presented. Pathogenesis of spontaneous rupture of urinary bladder is briefly discussed.
Autopsy ; Cystitis* ; Humans ; Military Personnel ; Pneumonia ; Rupture, Spontaneous* ; Ureter* ; Urinary Bladder*

This study was purosed to investigate maternal and fetal clinical parameters effecting on maternal serum alpha-fetoprotein(MSAFP) levels at late normal singleton pregnancies. The subjects of this study were 171 pregnant women with gestational age of 36 to 42 weeks, and didn`t have any medical or gynecologic diseases. They delivered fetuses within three days after blood test of MSAFP. MSAFP levels were measured by enzyme-immunoassay. The analysed clinical parameters included fetal sex, fetal weight, gestatioal age, maternal age, gravidity, parity, maternal weight and maternal total weight gain during pregnancy. The results were as follows: At uncomplicated late pregnancies, 1. Male fetus bearers had higher MSAFP(mean: 191.8 ng/ml, SD: 80.8 ng/ml, n=79) than female-fetus bearers(mean 153.6 ng/ml, SD 73.0 ng/ml, n=92)(p=0.0014). 2. Multiparas had higher MSAFP(mean: 192.7 ng/ml, SD: 77.9 ng/ml, n=80) than nulliparas(mean: 152.3 ng/ml, SD 75.1 ng/ml, n=91)(p=0.0007). 3. MSAFP did not have correlation with maternal age, maternal weight, maternal total wight gain during pregnancy, gestational age, fetal weight. According to the above results, fetal sex and parity are the factors that influences MSAFP levels at uncomplicated late pregnancies. So MSAFP values should be interpreted with cautions.
Female ; Fetal Weight ; Fetus ; Genital Diseases, Female ; Gestational Age ; Gravidity ; Hematologic Tests ; Humans ; Male ; Maternal Age ; Parity ; Pregnancy* ; Pregnant Women ; Weight Gain

OBJECT: This study was carried out to investigate the effect of pentoxifylline on in vitro fertillization and developmen of preimplantation stage of mouse embryos. MATERIAL AND METHODS:F1 hybrid mice was superovulated with PMSG/hCG and mouse oocytes were recruited. After the normal sperms were incubated with PTX before in vitro fertilization, it was observed whether the fertilization and embryo development was affected or not by the sperm preparation(washing, dilution and no washing or no dilution). And after 1-cell and 2-cell stage of mouse embryos were incubated with PTX, the development to hatching blastocyst was also observed. RESULTS: When in vitro fertilization was revealed by using the washed normal sperms after 0, 3.6 and 7.2 mM PTX incubation, the fertilization rates were 92.5%, 48.8%, 36.8%, respectively. So 3.6 and 7.2 mM groups presented significantly low fertilizatin rate, but the development rates were 93.9%, 85.0%, 95.2%, respectively. Therefore, there were no significant difference between each group. When in vitro fertilization was revealed by using the diluted normal sperms after 0, 3.6, and 7.2 mM PTX incubation, the fertilization rates were 58.6%, 5.4%, 9.4%, respectively. So 3.6 and 7.2 mM groups presented significantly low fertilization rate. The developmental rates were 88.2%, 100%, 100%. And there were no significant difference between each group. When in vitro fertilization was revealed by using the not washed and not diluted normal sperms after 0, 3.6 and 7.2 mM PTX incubation, the fertilizatin rates were 61.2%, 5.7%, 3.8%, respectively. 3.6 and 7.2 mM group presented significantly low fertilization rate. The development rates were 73.3%, 0%, 0%, respectively. So 3.6, 7.2 mM group presented significantly low developmental rate. After 1-cell stage of mouse embryos were incubated in 0, 5, 10, 50 nM of PTX, the development rates were not significantly different among them. After 2-cell stage of mouse embryos were incubated in 0, 5, 10, 50 nM of PTX, the development rates were not significantly different among them. CONCLUSION: In conclusion, when PTX is used in in vitro fertilization program with normal sperms, it may affect the fertilization and embryo development in high concentration. And if PTX concentration is very low, the developmental rate would not be affected. So PTX must not be used to normal sperms and where use of PTX is indicated, it is recommended that remainder PTX must be removed as completely as possible.
Animals ; Blastocyst ; Embryonic Development ; Embryonic Structures ; Female ; Fertilization ; Fertilization in Vitro* ; Mice* ; Oocytes ; Pentoxifylline* ; Pregnancy ; Spermatozoa*

No abstract available.
Humans ; Biomarkers, Tumor*

OBJECTIVES: Alcohol use disorder (AUD) is often comorbid with adult attention-deficit hyperactivity disorder (ADHD) and other psychiatric illnesses. When associated with other mental problems, the prognosis of the AUD can be more serious. This study shows research on the clinical and neuropsychological characteristics according to whether or not ADHD symptoms and AUD were comorbid. METHODS: MethodsA total of 64 adult inpatients who completed AUD scales about adult ADHD, alcohol dependence, depression, anxiety, and impulsiveness. They also completed neuropsychological tests about attention and executive function. According to the Adult ADHD Self-Report Scale score, patients were categorized into two groups (ADHD symptom positive/negative group). RESULTS: Fourteen among the 64 subjects were part of the ADHD symptom positive group (21.9%). They had statistically significant shorter abstinence periods and a higher rate of history of 'rule violation during school' and 'physical damage in childhood' compared to the ADHD symptom negative group. CONCLUSION: The comorbidity rate with ADHD symptom in adult AUD is higher than the general population. The ADHD symptom positive group suffered from more severe and refractory AUD. Considering the more frequent history of rule violation during school in the ADHD symptom positive group, the association between ADHD and AUD may be mediated by conduct disorder. Therefore we suggest the necessity of careful evaluation and intervention in children and adolescents with ADHD and conduct disorder.
Adolescent ; Adult* ; Alcoholism ; Anxiety ; Child ; Comorbidity ; Conduct Disorder ; Depression ; Executive Function ; Humans ; Impulsive Behavior ; Inpatients ; Neuropsychological Tests ; Prognosis ; Weights and Measures