Objective To explore the prognosis impact of lymph nodes metastasis in pancreatic cancer.Methods The clinical data of 61 patients with pancreatic cancer who underwent pancreaticoduodenectomy from January 2004 to September 2011 were analyzed retrospectively.Patients were categorized into different groups by lymph nodes metastasis status,count of positive lymph nodes,location of positive lymph nodes,lymph nodes ratio (LNR) and other factors.Kaplan-Meier,log-rank and COX proportional hazard models were used to evaluate the prognostic effect.Results Median survival for the overall 61 patients was 13 months (95％ CI 9.158-16.842),with 6 months,1 year,3 years,5 years survival rates of 84.6％,47.7％,14.3％ and 9.1％ respectively.Univariate analysis showed:the median survival of patients with and without lymph nodes metastasis had statistical significant outcome (P ＜ 0.01).Patients within the first station lymph nodes metastasis had a better outcome than those lymph nodes metastasis beyond the first station (P ＜0.05).Patients with LNR ≤0.2 had better prognosis than those LNR ＞ 0.2 (P ＜ 0.01).The number of lymph nodes examined had no effect on overall survival in either nodes-positive patients or nodes-negative patients (P ＞ 0.05).The count of positive lymph nodes did not affect the prognosis in the pN1 patients (P＞ 0.05).Multivariate analysis showed:location of positive lymph nodes and LNR were independent predictors of the prognosis in the pN1 patients.Conclusions Lymph nodes metastasis status has significant effect on the prognosis of the pancreatic cancer.Location of positive lymph nodes and LNR are independent predictors of the prognosis in the pN1 patients.The number of lymph nodes examined and the number of positive lymph nodes have no effect on the prognosis of the pancreatic cancer.

Anastomotic cicatricial stenosis after resection of esophageal cancer were treated by self- making electric knife through endoscipe.Fifty two cases were located in upper segment while 37 cases in middle-lowere part.Anastomotic diameter of 40 cases were less than 2.0mm,17 cases less than 3.0mm. Sixteen cases more than 3.0mm. Anastomotic stoma were incarcerated by food in 16 cases.The operatic procedure:First,the cicatricial stenosis was resected and became"*"shape by electric knife,then dilated by baloon catheter and by endoscope.sitytwo cases of stenosis were unobstrated(can swallow solid food)after one time treatment ; 11 cases each needed 2 and 3 times;1 case each needed 4 , 5 , 12 , 43and 81 times ,respec- tively. The last 2 cases were treated repeatly every 1- 3 months. The authors think that this procedure is ef- fective and safe. The technique is mastered easily and could be wildely used.

Objective:To investigate the comorbid depressive symptoms,anxiety symptoms and suicide risk in people with alcohol abuse.Methods:Totally 1992 community residents were recruited by probability proportionate to size sampling.They were assessed with the Alcohol Use Disorders Identification Test (AUDIT),Depression Sub-scale(PHQ-9 )and Anxiety Subscale (PHQ GAD-7 )of Patient Health Questionnaire,and Suicide Risk Screening Scale of Mini-International Neuropsychiatric Interview (MINI).Results:The AUDIT screening positive cases was 298 (15.1%).The rates of depression symptom (53.4% vs.42.3%),anxiety symptom (35.9% vs.27.0%)and suicide risk (5.7%vs.3.2%)in alcohol abuse group were all significantly higher than that in non-abuse group (Ps<0.0 1 ).Conclusion:It is important to conduct assessment of related psychological problems in screening of alco-hol abuse,in order to provide effective intervention timely.

Objective:To compare expansion and kill activation of A-NK cells cultured with serum-free medium AIMV and standard serum-containing medium in vitro, and also detest the assist effect of EL-12 on A-NK/IL-2 treatment and the morphology character of killed cells through electroscope.Methods:Proliferation ability of the cells cultured in the two different media was compared by MTT method in vitro. The morphology of the killed tumor target cells by adherent natural killer cells (A-NK) was observed through electroscope. Results:All of A-NK cells cultured in deferent condition could rapidly proliferate(P

OBJECTIVETo investigate the efficacy, advantages and disadvantages of internal and external elbow joint approach and olecranon osteotomy approach for the treatment of intercondylar fracture of humerus.

METHODSFrom October 2012 to May 2016, 18 cases of intercondylar fracture of humerus were treated by operation including 12 males and 6 females with a mean age of 33.5 years old (ranged from 4 to 56 years old); 8 cases were operated by internal and external elbow joint approach, 10 cases were operated by olecranon osteotomy approach. According to AO classification, 3 cases were type C1, 8 cases were type C2, 7 cases were type C3. All patients were excluded from neurologic and vascular injuries.

RESULTSAll patients were followed up from 12 to 26 months with an average of 15 months. The incision healed well and no heterotopic ossification was found. According to the modified Cassebaum elbow function score, the result was excellence in 14 cases, good in 3 cases, fair in 1 case.

CONCLUSIONSAccording to the fracture type, the appropriate surgical approach and fixation were selected in order to get anatomic reduction. Rigid fixation, and early functional exercise is important condition for successful operation and satisfactory functional recovery in intercondylar fracture of the humerus.

BACKGROUND:Osteopontin mRNA and protein expressions are highly correlated with the severity of osteoarthritis. OBJECTIVE:To investigate the effect of osteopontin on the gene expression of aggrecan and type II colagen in the human knee osteoarthritic chondrocytes in vitro. METHODS: Chondrocytes were harvested from human osteoarthritic knees and culturedin vitro. The chondrocytes were cultured with 0 (blank control group), 0.1, 1 mg/L osteopontin, respectively, for 48 hours. Real-time PCR was employed to detect the mRNA expression of aggrecan and type II colagen. RESULTS AND CONCLUSION:After 0.1 and 1 mg/L osteopontin intervention, the mRNA expression of aggrecan and type II colagen in osteoarthritic chondrocytes was increased significantly (P< 0.05), and the mRNA expression of aggrecan and type II colagen was higher in the 1 mg/L osteopontin group than the 0.1 mg/L osteopontin group (P< 0.05). In addition, the mRNA expression of aggrecan and type II colagen was positively correlated with the concentration of osteopontin (r=0.751,P < 0.01;r=0.676,P < 0.01). These findings indicate that osteopontin up-regulates the mRNA expression of aggrecan and type II colagen in osteoarthritic chondrocytes of human kneein vitro in a dose-dependent manner.

ObjectiveTo investigate the health status and incidence of alcohol abuse of Xinjiang community residents.MethodsThrough multi-stage random sampling,1992 community residents ( 15-65years old) were assessed using socio-demographic information questionnaire,alcohol use disorders identification test (AUDIT) and self-rated health measurement scale-prior test(SHRMS).Single factor analysis and rank sum test were used to identify the incidence of alcohol abuse,the distribution of different populations,and the self-rated health status of abusers.ResultsTotally 298 cases of alcohol abuse were identified,and accordingly,the prevalence rate of alcohol abuse for general population was 15.1％ ( male 26.4％,female 4.8％ ).Key factors for alcohol abuse included male,middle-aged,well-educated,at work,administrative work,high income levels and large number of household.The differences among groups were statistical significant.Presumably the workplace population was at high risk of alcohol abuse.Alcohol abusers were significantly worse than general population in self-rated health status(P ＜ 0.01 ),especially in mental health and social health.ConclusionThe incidence of alcohol abuse is relatively high in Xinjiang rcgion,and the problem need more attention.Further survey and early intervention work for highrisk group are advised to achieve the purpose of prevention and control.

Adult ; Aged ; Female ; Gastric Mucosa ; drug effects ; pathology ; Gastrointestinal Agents ; adverse effects ; therapeutic use ; Gastrointestinal Hemorrhage ; drug therapy ; etiology ; Humans ; Hypertension, Portal ; complications ; drug therapy ; Male ; Middle Aged ; Octreotide ; adverse effects ; therapeutic use ; Pituitary Hormones, Posterior ; adverse effects ; therapeutic use ; Treatment Outcome ; Vomiting ; chemically induced

Preparation of maternal strain A/PR/8/34 HA antiserum for influenza virus classical reassortment. A/PR/8/34 virus was digested by bromelain after inactivation and purification. 5%-20% sucrose continuous density gradient centrifugation method was used to purify HA protein. SIRD method was used to select the target protein. SDS-PAGE method was used to identified HA protein. High Immunogenic A/PR/8/34 HA protein was successfully prepared and HI titer reached 10240. High purity HA antiserum was identified by SIRD method. The key reagent in the classical reassortment of influenza virus was prepared, and the complete set of technical methods were explored, which laid the foundation for the independent research and development of seasonal influenza vaccine strains of China.
Animals ; Antibodies, Viral ; immunology ; Electrophoresis, Polyacrylamide Gel ; Female ; Hemagglutination Inhibition Tests ; Hemagglutinin Glycoproteins, Influenza Virus ; analysis ; immunology ; Humans ; Influenza A Virus, H1N1 Subtype ; genetics ; immunology ; Influenza, Human ; immunology ; virology ; Rabbits ; Reassortant Viruses ; genetics ; immunology

A simple and sensitive assay for rapid detection of human coronavirus NL63 (HCoV-NL63) was developed by colorimetic reverse transcription loop-mediated isothermal amplification (RT-LAMP). The method employed six specially designed primers that recognized eight distinct regions of the HCoV-NL63 nucleocapsid protein gene for amplification of target sequences under isothermal conditions at 63 degrees C for 1 h Amplification of RT-LAMP was monitored by addition of calcein before amplification. A positive reaction was confirmed by change from light-brown to yellow-green under visual detection. Specificity of the RT-LAMP assay was validated by cross-reaction with different human coronaviruses, norovirus, influenza A virus, and influenza B virus. Sensitivity was evaluated by serial dilution of HCoV-NL63 RNA from 1.6 x 10(9) to 1.6 x 10(1) per reaction. The RT-LAMP assay could achieve 1,600 RNA copies per reaction with high specificity. Hence, our colorimetric RT-LAMP assay could be used for rapid detection of human coronavirus NL63.
Colorimetry ; methods ; Coronavirus Infections ; diagnosis ; virology ; Coronavirus NL63, Human ; genetics ; isolation & purification ; DNA Primers ; genetics ; Humans ; Nucleic Acid Amplification Techniques ; methods ; Reverse Transcription ; Sensitivity and Specificity